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| These Kitl mutant mice carry a floxed exon which, when excised through a cross with a cre strain, enables tissue-specific disruption of gene expression. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Former Names BKa.B6(Cg)-Kitltm2.1Sjm/J (Changed: 24-FEB-12 ) BKa.B6-Kitltm2.1Sjm/J (Changed: 17-FEB-12 ) Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Heterozygote x Heterozygote (Female x Male) 14-DEC-12 Species laboratory mouse Generation ?pN1+ (01-MAR-13)
Generation DefinitionsDonating Investigator Sean J Morrison, UT Southwestern Description
These mice possess loxP sites on either side of exon 1 of the Kitl (kit ligand; also called Scf, stem cell factor) gene. Mice that are homozygous for this allele are viable and fertile with no observed abnormalities. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will have exon 1 deleted in cre-expressing tissues.Mice generated from a cross of these mice with Ubc-creER animals (e.g. Stock No. 008085) can be treated with tamoxifen to induce widespread deletion of the floxed segment. These mice become anemic and show significantly lower red blood cell counts, trend towards lower white blood cell and pletelet counts, and exhibit approximately twofold reductions in the overall cellularity of bone marrow and spleen.
Development
A loxP site was introduced 5' of exon 1 and an Frt-Neo-Frt-loxP cassette was placed 3' of exon 1 in sites that do not disrupt regulatory elements of the targeted gene. C57BL/6-derived Bruce 4 G9 embryonic stem (ES) cells were used to create the mutation. Chimeric mice were bred with C57BL/6-Tyrc-2J mice to obtain germline transmission. Animals were then crossed with C57BL/Ka background Flp-expressing mice to excise the neomycin cassette, leaving exon 1 flanked by loxP sites. This strain was backcrossed to C57BL/Ka for six generations by the donating laboratory. Upon arrival at The Jackson Laboratory, C57BL/6J was used for rederivation.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Kitl
000090 129S1/Sv-Oca2+ Tyr+ KitlSl-J/J 002993 B6.Cg-KitlSl-18H/EiJ 008656 B6.Cg-KitlSl-gb/MbeJ 000124 B6.Cg-KitlSl Krt71Ca/J 009687 B6.Cg-Tg(KRT14-Kitl*)4XTG2Bjl/J 000160 B6.D2-KitlSl-d/J 014608 B6;129S1-a Kitlsl-24J/GrsrJ 000291 C3FeLe.Cg-a/a Hm KitlSl Krt71Ca-J/J 001380 C3Sn.Cg-KitlSl-con/J 003252 C57BL/6J-KitlSl-20J/J 000979 STOCK KitlSl-16J/J 017860 STOCK Kitltm1.1Sjm/J 000161 WB.D2-KitlSl-d/J 000693 WC/ReJ KitlSl/J 100401 WCB6F1/J-KitlSl-d/J)F1-KitlSl/KitlSl-d/J 001145 WSB/EiJ View Strains carrying other alleles of Kitl (16 strains)
Phenotype
Phenotype Information
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Hyperpigmentation, Familial Progressive, 2; FPH2 (KITLG)
Skin/Hair/Eye Pigmentation, Variation In, 7; SHEP7 (KITLG)
assigned by genotype
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.Kitltm2.1Sjm/Kitltm2.1Sjm Tg(UBC-cre/ERT2)1Ejb/0
involves: 129S/SvEv * C57BL/Ka * C57BL/6 * SJL (conditional)
- hematopoietic system phenotype
- abnormal bone marrow cell physiology
- bone marrow cells from tamoxifen-treated mice used to repopulate irradiated mice exhibit reduced bone marrow-derived cell production (total, myeloid, T cell, and B cell) (MGI Ref ID J:180431)
- anemia
- in tamoxifen-treated mice (MGI Ref ID J:180431)
- decreased bone marrow cell number
- bone marrow cells from tamoxifen-treated mice used to repopulate irradiated mice exhibit reduced bone marrow-derived cell production (total, myeloid, T cell, and B cell) (MGI Ref ID J:180431)
- decreased hematopoietic cell number
- in tamoxifen-treated mice (MGI Ref ID J:180431)
- decreased erythrocyte cell number
- in tamoxifen-treated mice (MGI Ref ID J:180431)
- liver/biliary system phenotype
- liver hypoplasia
- in tamoxifen-treated mice (MGI Ref ID J:180431)
This mouse can be used to support research in many areas including:
Research Tools
Cre-lox System
loxP-flanked Sequences
Hematological Research
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Kitltm2.1Sjm | ||
|---|---|---|---|
| Allele Name | targeted mutation 2.1, Sean J Morrison | ||
| Allele Type | Targeted (Floxed/Frt) | ||
| Common Name(s) | Scffl; | ||
| Mutation Made By | Sean Morrison, UT Southwestern | ||
| Strain of Origin | B6.Cg-Thy1 | ||
| Gene Symbol and Name | Kitl, kit ligand | ||
| Chromosome | 10 | ||
| Gene Common Name(s) | Clo; Con; FPH2; Gb; KL-1; MGF; Mgf; SCF; SF; SHEP7; SLF; Sl; Steel; Steel factor; blaze; blz; cloud gray; contrasted; grizzle-belly; mast cell growth factor; steel; stem cell factor; | ||
| Molecular Note | An FRT-flanked neo cassette with loxP site was inserted downstream of exon 1 and an additional loxP site was inserted upstream of exon 1. The neo cassette was removed by flp-mediated recombination. [MGI Ref ID J:180431] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Kitltm2.1Sjm, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Ding L; Saunders TL; Enikolopov G; Morrison SJ. 2012. Endothelial and perivascular cells maintain haematopoietic stem cells. Nature 481(7382):457-62. [PubMed: 22281595] [MGI Ref ID J:180431]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number MGL375
Colony Maintenance
Breeding & Husbandry Both heterozygotes and homozygotes are viable and fertile. Mating System Heterozygote x Heterozygote (Female x Male) 14-DEC-12
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Heterozygous for Kitltm2.1Sjm $232.00 Female or Male Homozygous for Kitltm2.1Sjm
Price per Pair (US dollars $) Pair Genotype $464.00 Heterozygous for Kitltm2.1Sjm x Heterozygous for Kitltm2.1Sjm Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Heterozygous for Kitltm2.1Sjm $301.60 Female or Male Homozygous for Kitltm2.1Sjm
Price per Pair (US dollars $) Pair Genotype $603.20 Heterozygous for Kitltm2.1Sjm x Heterozygous for Kitltm2.1Sjm Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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