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| These mutant mice possess loxP sites flanking exons 4 to 8 of the leukocyte-associated Ig-like receptor 1 (Lair1) gene, an inhibitory receptor protein, and may be useful for studying immune cell regulation. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names C57BL/6-Lair1tm1Jco/J (Changed: 07-MAR-12 ) Type Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation +pN1
Generation DefinitionsDonating Investigator John E Coligan, NIAID/NIH Description
LAIR1, an inhibitory receptor protein with a high affinity for collagen, is expressed on most cells of the immune system and is associated with inhibition of the cytotoxic activity of NK and CD8+ T cells, BCR-induced B cell activation and proliferation, and CD3 signaling and cytokine production by T cells. These mutant mice possess loxP sites flanking exons 4 to 8 of the (Lair1) gene. Mice that are homozygous for this allele are viable, fertile, and normal in size. When bred to mice that express Cre recombinase, resulting offspring will have exons 4-8 deleted in the cre-expressing tissues. This strain may be useful for studying immune cell regulation.Development
A targeting vector was designed to insert a loxP site upstream of exon 4 followed by a frt-flanked neomycin resistance (neo) cassette and a second loxP site downstream of exon 8 of the Lair1 gene. Exons 4-8 contain two immunoreceptor tyrosine-based inhibition motifs (ITIM)that interact with the tyrosine phosphatases SHP1, SHP2 and SRC kinase. This construct was electroporated into C57BL/6-derived Bruce4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric mice were bred to C57BL/6 mice.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Phenotype
Phenotype Information
View Mammalian Phenotype Terms
Mammalian Phenotype Terms provided by MGI
assigned by genotype
Lair1tm1Jco/Lair1tm1Jco
B6.Cg-Lair1tm1Jco
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:180203)
This mouse can be used to support research in many areas including:
Research Tools
Cre-lox System
loxP-flanked Sequences
Immunology and Inflammation Research
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Lair1tm1Jco | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, John E. Coligan | ||
| Allele Type | Targeted (Floxed/Frt) | ||
| Common Name(s) | LAIR-1fl/fl; | ||
| Mutation Made By | John Coligan, NIAID/NIH | ||
| Strain of Origin | B6.Cg-Thy1 | ||
| Gene Symbol and Name | Lair1, leukocyte-associated Ig-like receptor 1 | ||
| Chromosome | 7 | ||
| Gene Common Name(s) | 5133400O11Rik; BB115266; CD305; D7Bwg0421e; DNA segment, Chr 7, Brigham & Women's Genetics 0421 expressed; LAIR-1; RIKEN cDNA 5133400O11 gene; expressed sequence BB115266; mLair-1; | ||
| Molecular Note | A targeting vector was designed to insert a loxP site upstream of exon 4 followed by a frt-flanked neomycin resistance (neo) cassette and a second loxP site downstream of exon 8 via homologous recombination. Exons 4-8 contain two immunoreceptor tyrosine-based inhibition motifs (ITIM)that interact with the tyrosine phosphatases SHP1, SHP2 and SRC kinase. [MGI Ref ID J:180203] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Lair1tm1Jco, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Tang X; Tian L; Esteso G; Choi SC; Barrow AD; Colonna M; Borrego F; Coligan JE. 2012. Leukocyte-associated Ig-like receptor-1-deficient mice have an altered immune cell phenotype. J Immunol 188(2):548-58. [PubMed: 22156345] [MGI Ref ID J:180203]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry While maintaining a live colony, these mice are bred as homozygotes
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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| phone: | 207-288-6470 |
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