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| The R26TT allele has a CMV enhancer/chicken beta-actin core promoter-driven "MADM TT" cassette inserted into the Gt(ROSA)26Sor locus on chromosome 6. These R26TT mice exhibit widespread expression of tdTomato-3Myc (tdT3Myc) and represent the tdTomato-expressing control strain for "new MADM-6" (new mosaic analysis with double markers on chromosome 6) experiments. | |||||||||||||||||||
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Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Dr. Liqun Luo, Stanford University, HHMI Description
Mice homozygous for the R26TT allele are viable and fertile with no gross behavioral or observable abnormalities. The R26TT allele has a CMV enhancer/chicken beta-actin core promoter-driven "MADM TT" cassette inserted into the Gt(ROSA)26Sor locus on chromosome 6. The "MADM TT" cassette has a complete tdTomato sequence (tagged with three copies of the Myc epitope at its C-terminus). As such, heterozygous and homozygous mice exhibit widespread expression of tdTomato-3Myc (direct fluorescent visualization) throughout the whole animal and all tissues. These R26TT mice are the tdTomato-expressing control for the "new MADM-6" (new mosaic analysis with double markers on chromosome 6) strains, including R26GT (Stock No. 017912) and R26TG (Stock No. 017921). These R26TT mice do not need to be bred with any mice harboring a reciprocal mutation at the same locus to obtain tdTomato expression. A detailed description and figure showing how R26TT mice were generated is available here.Development
These R26TT mice have the CMV enhancer/chicken beta-actin core promoter (pCA) upstream of the recombined "MADM TT" cassette inserted into the Gt(ROSA)26Sor locus. The recombined "MADM TT" cassette has an ATG start codon, a beta-globin intronic sequence (containing a frt-flanked region of two lox site variants [lox2272 and lox5171] followed by a single loxP site), an ATG-less tdTomato sequence (nucleotides 3-1548 including stop codon) tagged with three copies of the Myc epitope at its C-terminus, and an SV40 T-antigen poly(A) signal.To generate these R26TT mice, the donating investigator bred R26GT mice (Stock No. 017912) with HprtCre mice. The double mutant progeny were then bred with R26TG mice (Stock No. 017921). The resulting triple mutant offspring (R26GT/TG;HprtCre) were next bred with wildtype mice. Cre-mediated interchromosomal recombination in meiosis resulted in mice with the R26TT;HprtCre/+ or R26TT genotype. The resulting R26TT animals were then bred together (and the HprtCre was selectively bred out of the line) for several generations. The R26TT mice on a mixed genetic background (mixed CD1, 129, C57BL/6 and/or FVB/N) were sent to The Jackson Laboratory Repository. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish The Jackson Laboratory Repository colony.
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Phenotype
Phenotype Information
assigned by genotype
Gt(ROSA)26Sortm10(ACTB-tdTomato)Luo/Gt(ROSA)26Sor+
involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CD-1
- no phenotypic analysis
- *normal* no phenotypic analysis (MGI Ref ID J:181830)
This mouse can be used to support research in many areas including:
Cell Biology Research
Cell Cycle Regulation
Genes Regulating Growth and Proliferation
Neurobiology Research
Cre-lox System
loxP-flanked Sequences
loxP-flanked Sequences: Test/Reporter
Fluorescent protein expression in neural tissue
Research Tools
Cardiovascular Research
Cre-lox System
Cell Biology Research
Cre-lox System
loxP-flanked Sequences
loxP-flanked Sequences: Test/Reporter
Developmental Biology Research
Cre-lox System
Diabetes and Obesity Research
loxP
FLP-FRT System
FRT-flanked Sequences
FRT-flanked Sequences: Test/Reporter
Fluorescent Proteins
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
Neurobiology Research
cell marker
Reproductive Biology Research
Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Gt(ROSA)26Sortm10(ACTB-tdTomato)Luo | ||
|---|---|---|---|
| Allele Name | targeted mutation 10, Liqun Luo | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | R26TT; | ||
| Mutation Made By | Dr. Liqun Luo, Stanford University, HHMI | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | These mice have widespread tdTomato expression when using the "new MADM-6" system. | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
| Molecular Note |
Cre-mediated recombination between | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(ROSA)26Sortm6,7,8,10(ACTB-EFGP*,-tTA2*,-tdTomato*LuoMCA,Separated MCA
Gt(ROSA)26Sortm6,7,8,10(ACTB-EFGP*,-tTA2*,-tdTomato*Luo, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Additional References
Gt(ROSA)26Sortm10(ACTB-tdTomato)Luo relatedTasic B; Miyamichi K; Hippenmeyer S; Dani VS; Zeng H; Joo W; Zong H; Chen-Tsai Y; Luo L. 2012. Extensions of MADM (Mosaic Analysis with Double Markers) in Mice PLoS ONE 7(3):e33332. [PubMed: 22479386] [MGI Ref ID J:181830]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice may be bred together.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2250.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2925.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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