Strain Name:

STOCK Tg(Krt8-cre/ERT2)17Blpn/J

Stock Number:

017947

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Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
These K8-CreER transgenic mice express the Cre-ERT2 fusion gene under control of the mouse Krt8 promoter. They may have applications related to lineage tracing. When bred with mice containing a loxP-flanked sequence, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in mammary gland luminal epithelial cells.

Description

Strain Information

Type Mutant Stock; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
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Mating SystemNoncarrier x Hemizygote         (Female x Male)   20-AUG-12
Mating SystemHemizygote x Noncarrier         (Female x Male)   20-AUG-12
Specieslaboratory mouse
GenerationF?pN1+N2F2 (11-DEC-13)
Generation Definitions
 
Donating Investigator Cedric Blanpain,   Universit┐ Libre de Bruxelles

Description
Keratin 8 is an intermediate filament cytoskeleton protein and is protective in TNF- and Fas-mediated apoptosis. Merkel cells are neuroendocrine cells found in touch domes (mechanoreceptor zones in the skin) of the epithelial basal layer and some mucosa. Merkel cells express both intermediate filaments and neuropeptides. These transgenic mice express CreERT2 under the direction of the mouse Krt8, keratin 8, promoter. When these K8-CreER mice, founder line 17, are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in Merkel cells and simple epithelia. When bred to ROSA-YFP reporter mice, pulse/chase adminstration of tamoxifen results in the fluorescent labeling of mammary gland luminal epithelial cells. Mice that are homozygous for the targeted mutation are viable, fertile and normal in size. The Donating Investigator reports that there is no difference in phenotype between hemizygotes and homozygotes.

Development
A transgenic construct containing Cre-ERT2 under the control of the mouse Krt8, keratin 8, promoter, beta-globin intron and SV40 polyadenylation site sequence was injected into fertilized C57BL/6 mouse eggs. Founder line 17 was subsequently established. The transgenic animals were then bred to CD1 mice. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Krt8
007031   FVB.Cg-Krt8tm1Rgo/J
View Strains carrying other alleles of Krt8     (1 strain)

View Strains carrying other alleles of cre/ER     (10 strains)

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Research Tools
Cre-lox System
      Cre Recombinase Expression: Inducible
Genetics Research
      Mutagenesis and Transgenesis: Cre-lox System

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(Krt8-cre/ERT2)17Blpn
Allele Name transgene insertion 17, Cedric Blanpain
Allele Type Transgenic (Inducible, Recombinase (cre or Flp) expressing)
Common Name(s) K8-CreER;
Site of ExpressionWhen mice carrying this transgene are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in Merkel cells and simple epithelia.
Expressed Gene cre/ER, cre inducible estrogen receptor, bacteriophage P1/Human
Promoter Krt8, keratin 8, mouse, laboratory
Driver Note Krt8
Inducible Note induced by tamoxifen
Molecular Note A transgene construct containing 3.5 kb of sequence upstream of the ATG start site of the mouse Krt8 gene (purified from BAC clone RP23-254K21), the beta globin intron, SV40 polyadenylation site and a cre/ERT2 segment was injected into C57Bl/6 fertilizedeggs. Four of seven founder mice displayed the expected pattern of expression and Founder line 17 was subsequently established. The transgenic animals were then bred to CD1 mice. [MGI Ref ID J:180048]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Van Keymeulen A; Rocha AS; Ousset M; Beck B; Bouvencourt G; Rock J; Sharma N; Dekoninck S; Blanpain C. 2011. Distinct stem cells contribute to mammary gland development and maintenance. Nature 479(7372):189-93. [PubMed: 21983963]  [MGI Ref ID J:180048]

Additional References

Tg(Krt8-cre/ERT2)17Blpn related

Ousset M; Van Keymeulen A; Bouvencourt G; Sharma N; Achouri Y; Simons BD; Blanpain C. 2012. Multipotent and unipotent progenitors contribute to prostate postnatal development. Nat Cell Biol 14(11):1131-8. [PubMed: 23064263]  [MGI Ref ID J:193931]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX18

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as homozygotes.
Mating SystemNoncarrier x Hemizygote         (Female x Male)   20-AUG-12
Hemizygote x Noncarrier         (Female x Male)   20-AUG-12
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHemizygous for Tg(Krt8-cre/ERT2)17Blpn  
Price per Pair (US dollars $)Pair Genotype
$304.00Hemizygous for Tg(Krt8-cre/ERT2)17Blpn x Noncarrier  
$304.00Noncarrier x Hemizygous for Tg(Krt8-cre/ERT2)17Blpn  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHemizygous for Tg(Krt8-cre/ERT2)17Blpn  
Price per Pair (US dollars $)Pair Genotype
$395.20Hemizygous for Tg(Krt8-cre/ERT2)17Blpn x Noncarrier  
$395.20Noncarrier x Hemizygous for Tg(Krt8-cre/ERT2)17Blpn  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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