Strain Name:

STOCK Tg(KRT18-cre/ERT2)23Blpn/J

Stock Number:

017948

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Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
These transgenic mice express a tamoxifen-inducible cre recombinase from the human KRT18, keratin 18, promoter. When induced, cre activity is observed in Merkel cells of the skin and simple epithelia. They may be useful in lineage tracing.

Description

Strain Information

Type Mutant Stock; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
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Mating SystemNoncarrier x Hemizygote         (Female x Male)   20-AUG-12
Mating SystemHemizygote x Noncarrier         (Female x Male)   20-AUG-12
Specieslaboratory mouse
GenerationF?pN1+F3 (10-DEC-13)
Generation Definitions
 
Donating Investigator Cedric Blanpain,   Université Libre de Bruxelles

Description
Keratin 18 is an intermediate filament cytoskeleton protein found in adult simple epithelial tissues. Merkel cells are neuroendocrine cells found in touch domes (mechanoreceptor zones in the skin) of the epithelial basal layer and some mucosa. Merkel cells express both intermediate filaments and neuropeptides. These transgenic mice express CreERT2 under the direction of the human KRT18, keratin 18, promoter. When these K18-CREER#23 mice are bred with mice containing a loxP-flanked sequence, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in Merkel cells and simple epithelia. When bred to ROSA-YFP reporter mice, pulse/chase adminstration of tamoxifen results in the fluorescent labeling of 17% of vibrissa Merkel cells 1 week after pulse, and 9% after 3 weeks. Mice that are hemizygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make this strain homozygous.

Development
A transgenic construct containing Cre-ERT2 under the control of the human KRT18, keratin 18, promoter, SV40 intron and SV40 polyadenylation site sequence was injected into fertilized C57BL/6 mouse eggs. Founder line 23 was subsequently established. The transgenic animals were then bred to CD1 mice. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of cre/ER     (10 strains)

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Cre-lox System
      Cre Recombinase expression in neural tissue

Research Tools
Cre-lox System
      Cre Recombinase Expression: Inducible
Genetics Research
      Mutagenesis and Transgenesis: Cre-lox System
      Tissue/Cell Markers: Cre-lox System

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(KRT18-cre/ERT2)23Blpn
Allele Name transgene insertion 23, Cedric Blanpain
Allele Type Transgenic (Cre/Flp)
Common Name(s) K18-CREER;
Mutation Made By Cedric Blanpain,   Université Libre de Bruxelles
Strain of OriginC57BL/6
Site of ExpressionWhen mice carrying this transgene are bred with mice containing a loxP-flanked sequence, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in Merkel cells and simple epithelia.
Expressed Gene cre/ER, cre inducible estrogen receptor, bacteriophage P1/Human
Promoter KRT18, keratin 18, human
Driver Note KRT18
Inducible Note induced by tamoxifen
Molecular Note A fragment encoding cre recombinase fused to a modified estrogen receptor ligand-binding domain (ERT2) was cloned into a vector containing the human KRT18 promoter. The construct was inected into fertilized oocytes to generated transgenic mice. Two lineswere obtained. Line 23 was used in the original reference. [MGI Ref ID J:163216]
 
Gene Symbol and Name Tg(KRT18-cre/ERT2)23Blpn, transgene insertion 23, Cedric Blanpain
Chromosome UN
Gene Common Name(s) K18-CREER;
 

Genotyping

Genotyping Information

Genotyping Protocols

Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Van Keymeulen A; Mascre G; Youseff KK; Harel I; Michaux C; De Geest N; Szpalski C; Achouri Y; Bloch W; Hassan BA; Blanpain C. 2009. Epidermal progenitors give rise to Merkel cells during embryonic development and adult homeostasis. J Cell Biol 187(1):91-100. [PubMed: 19786578]  [MGI Ref ID J:163216]

Additional References

Tg(KRT18-cre/ERT2)23Blpn related

Lu CP; Polak L; Rocha AS; Pasolli HA; Chen SC; Sharma N; Blanpain C; Fuchs E. 2012. Identification of stem cell populations in sweat glands and ducts reveals roles in homeostasis and wound repair. Cell 150(1):136-50. [PubMed: 22770217]  [MGI Ref ID J:186240]

Ousset M; Van Keymeulen A; Bouvencourt G; Sharma N; Achouri Y; Simons BD; Blanpain C. 2012. Multipotent and unipotent progenitors contribute to prostate postnatal development. Nat Cell Biol 14(11):1131-8. [PubMed: 23064263]  [MGI Ref ID J:193931]

Van Keymeulen A; Rocha AS; Ousset M; Beck B; Bouvencourt G; Rock J; Sharma N; Dekoninck S; Blanpain C. 2011. Distinct stem cells contribute to mammary gland development and maintenance. Nature 479(7372):189-93. [PubMed: 21983963]  [MGI Ref ID J:180048]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX18

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, hemizygous mice may be bred together, to wildtype siblings, or to C57BL/6J inbred mice (Stock No. 000664). The Donating Investigator has not attempted to make this strain homozygous.
Mating SystemNoncarrier x Hemizygote         (Female x Male)   20-AUG-12
Hemizygote x Noncarrier         (Female x Male)   20-AUG-12
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHemizygous for Tg(KRT18-cre/ERT2)23Blpn  
Price per Pair (US dollars $)Pair Genotype
$302.00Hemizygous for Tg(KRT18-cre/ERT2)23Blpn x Noncarrier  
$302.00Noncarrier x Hemizygous for Tg(KRT18-cre/ERT2)23Blpn  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHemizygous for Tg(KRT18-cre/ERT2)23Blpn  
Price per Pair (US dollars $)Pair Genotype
$392.60Hemizygous for Tg(KRT18-cre/ERT2)23Blpn x Noncarrier  
$392.60Noncarrier x Hemizygous for Tg(KRT18-cre/ERT2)23Blpn  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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