Strain Name:

B6(Cg)-Dpp6tm1.1Dahn/J

Stock Number:

017972

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Availability:

Cryopreserved - Ready for recovery

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These dipeptidylpeptidase 6 (Dpp6) knockout mice exhibit a loss of A-type K+ current in distal dendrites and may be useful in studies of dendritic function and potassium currents.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
 
Donating Investigator Bernardo Rudy,   NYU School of Medicine

Description
The transmembrane auxillary subunit, dipeptidylpeptidase 6 (Dpp6), is expressed in dendrites of CA1 pyramidal neurons and other areas of the brain. It is an auxiliary subunit of potassium channels containing Kv4 (Kcnd) pore forming subunits. DPP6 regulates the expression of Kv4.2 (Kcnd2) as well as the subcellular pattern of expression and properties of Kv4-containing A-type K+ channels. CA1 pyramidal neuron apical dendrites in mice homozygous for this null allele exhibit a loss of A-type K+ current in distal dendrites, an increased depolarizing shift in the inactivation curve of proximal dendrites, and a slower recovery from inactivation. These effects result in hyperexcitable dendrites with enhanced backpropagation of action potentials and long term potentiation. Mice that are homozygous for the targeted mutation are viable, fertile, normal in size. This mutant mouse strain may be useful in studies of dendritic function, potassium currents and their relation to behavior.

Development
A targeting vector was designed to insert a loxP site upstream of exon 2 followed by a frt-flanked neomycin resistance (neo) cassette, and a second loxP site downstream of exon 2 of the dipeptidylpeptidase 6 (Dpp6) gene. The construct was electroporated into C57BL/6-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric mice were bred to C57BL/6 mice. The donating investigator reported that mice were crossed to a Cre deleter strain on a C57BL/6 background (see SNP note below) to remove exon 2 and the neo cassette. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 4 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Ventricular Fibrillation, Paroxysmal Familial, 2; VF2   (DPP6)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Dpp6tm1.1Dahn/Dpp6tm1.1Dahn

        involves: C57BL/6
  • nervous system phenotype
  • abnormal afterhyperpolarization
    • enhanced due to slower A-current inactivation during repolarization   (MGI Ref ID J:178547)
  • abnormal synaptic transmission
    • CA1 pyramidal neuron apical dendrites are hyperexcitable compared with wild-type neurons   (MGI Ref ID J:178547)
    • however, somatic membrane excitability and GABAergic currents are normal   (MGI Ref ID J:178547)
    • abnormal channel response
      • mice lack the distal dendritic A-type potassium current gradient compared with wild-type mice   (MGI Ref ID J:178547)
      • calcium electrogenesis is enhanced in CA1 dendrites compared to in wild-type cells   (MGI Ref ID J:178547)
      • remaining A-type potassium channels in dendrites are Kcna4 mediated   (MGI Ref ID J:178547)
    • abnormal long term potentiation
      • neurons exhibit extended spike-timing window for long term potentiation compared with wild-type neurons   (MGI Ref ID J:178547)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Channel and Transporter Defects
      potassium

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Dpp6tm1.1Dahn
Allele Name targeted mutation 1.1, Dax A Hoffman
Allele Type Targeted (Null/Knockout)
Mutation Made By Bernardo Rudy,   NYU School of Medicine
Strain of OriginB6.Cg-Thy1
Gene Symbol and Name Dpp6, dipeptidylpeptidase 6
Chromosome 5
Gene Common Name(s) B930011P16Rik; DPP VI; DPPX; Dpp-6; Gm1377; In(5)6H-p; LOC384168; Peplb; RIKEN cDNA B930011P16 gene; Rw; VF2; gene model 1377, (NCBI); inversion, Chr 5, Harwell 6, proximal; peptidase-like brain protein;
Molecular Note A loxP site was inserted upstream of exon 2. An FRT-flanked neo cassette with a 3' loxP site was inserted downstream of exon 2. Cre-mediated recombination removed exon 2 and the neo cassette. Immunohistochemistry confirmed the absence of protein expression in the brain. [MGI Ref ID J:178547]

Genotyping

Genotyping Information

Genotyping Protocols

Dpp6tm1.1Dahnalternate2, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Sun W; Maffie JK; Lin L; Petralia RS; Rudy B; Hoffman DA. 2011. DPP6 establishes the A-type K(+) current gradient critical for the regulation of dendritic excitability in CA1 hippocampal neurons. Neuron 71(6):1102-15. [PubMed: 21943606]  [MGI Ref ID J:178547]

Additional References

Dpp6tm1.1Dahn related

Maffie JK; Dvoretskova E; Bougis PE; Martin-Eauclaire MF; Rudy B. 2013. Dipeptidyl-peptidase-like-proteins confer high sensitivity to the scorpion toxin AmmTX3 to Kv4-mediated A-type K+ channels. J Physiol 591(Pt 10):2419-27. [PubMed: 23440961]  [MGI Ref ID J:210579]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhile maintaining a live colony, these mice are bred as homozygotes.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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