Strain Name:

B6.129-Ulk2tm1Thsn/J

Stock Number:

017977

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Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
These floxed Ulk2FL mice may be useful for studying the mechanisms of autophagy.

Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Additional information on Congenic nomenclature.
Mating SystemHomozygote x Homozygote         (Female x Male)   25-JUL-13
Specieslaboratory mouse
GenerationN10p+N2F (17-JAN-13)
Generation Definitions
 
Donating Investigator Craig B. Thompson,   Memorial Sloan Kettering Cancer Center

Description
ULK2 is an evolutionarily conserved serine threonine kinase which plays a role in the regulation of neurotransmitter trafficking and axonal growth. ULK2 is a homolog of yeast autophagy-related gene (Atg1), which has been implicated in organelle autophagy during cell growth and survival.These Ulk2FL mutant mice possess a loxP site upstream of exon 1, and a loxP-flanked neomycin resistance (neo) cassette downstream of exon 3. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice that express Cre recombinase, resulting offspring will have exons 1-3, and the neo cassette, deleted in cre-expressing tissues. ULK2-deficient mice have no overt autophagy phenotype. When these floxed mice are bred to B6;129-Ulk1tm1Kund/J mice (Stock No. 017976), and subsequently to mice that express Cre recombinase, double KO mice die within 24 hours of birth. When mouse embryonic fibroblasts (MEFs) are cultured from these double KO mice, amino-acid deprivation does not induce autophagy, while normal autophagy is evident during glucose deprivation and ammonia induction.

Development
A targeting vector was designed to insert a loxP site upstream of exon 1, and a loxP-flanked neomycin resistance (neo) cassette downstream of exon 3 of the Unc-51 like kinase 2 (Ulk2) gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric males were bred with C57BL/6J females. These mice were backcrossed to C57BL/6J mice for at least 10 generations. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Ulk2tm1Thsn/Ulk2tm1Thsn

        B6.129-Ulk2tm1Thsn
  • normal phenotype
  • no abnormal phenotype detected
    • mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities   (MGI Ref ID J:181236)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cell Biology Research
Protein Processing
      degradation

Neurobiology Research
Cre-lox System
      loxP-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Ulk2tm1Thsn
Allele Name targeted mutation 1, Craig B Thompson
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) Ulk2FL;
Mutation Made By Craig Thompson,   Memorial Sloan Kettering Cancer Center
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name Ulk2, unc-51 like kinase 2
Chromosome 11
Gene Common Name(s) A830085I22Rik; ATG1B; AU015340; RIKEN cDNA A830085I22 gene; Unc51.2; expressed sequence AU015340; mKIAA0623;
Molecular Note A targeting vector was designed to insert a loxP site upstream of exon 1, and a loxP-flanked neomycin resistance (neo) cassette downstream of exon 3. [MGI Ref ID J:174297]

Genotyping

Genotyping Information

Genotyping Protocols

Generic Cre Melt Curve Analysis,

MELT


Generic Cre Melt Curve Analysis,

Probe


Ulk2tm1Thsn,

Separated MCA


Ulk2tm1Thsn, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Cheong H; Lindsten T; Wu J; Lu C; Thompson CB. 2011. Ammonia-induced autophagy is independent of ULK1/ULK2 kinases. Proc Natl Acad Sci U S A 108(27):11121-6. [PubMed: 21690395]  [MGI Ref ID J:174297]

Additional References

Ulk2tm1Thsn related

Thompson CB. 2012. Direct Data Submission 2012/03/13 MGI Direct Data Submission :.  [MGI Ref ID J:181236]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX18

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice may be bred together.
Mating SystemHomozygote x Homozygote         (Female x Male)   25-JUL-13
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHomozygous for Ulk2tm1Thsn  
Price per Pair (US dollars $)Pair Genotype
$464.00Homozygous for Ulk2tm1Thsn x Homozygous for Ulk2tm1Thsn  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHomozygous for Ulk2tm1Thsn  
Price per Pair (US dollars $)Pair Genotype
$603.20Homozygous for Ulk2tm1Thsn x Homozygous for Ulk2tm1Thsn  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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