Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Heterozygote x Heterozygote         (Female x Male)   21-JUN-12 Species laboratory mouse Generation N10p+N2F (17-JAN-13) Generation Definitions   Donating Investigator Craig B. Thompson,   Memorial Sloan Kettering Cancer Center

Description
ULK2 is an evolutionarily conserved serine threonine kinase which plays a role in the regulation of neurotransmitter trafficking and axonal growth. ULK2 is a homolog of yeast autophagy-related gene (Atg1), which has been implicated in organelle autophagy during cell growth and survival.These Ulk2^FL mutant mice possess a loxP site upstream of exon 1, and a loxP-flanked neomycin resistance (neo) cassette downstream of exon 3. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice that express Cre recombinase, resulting offspring will have exons 1-3, and the neo cassette, deleted in cre-expressing tissues. ULK2-deficient mice have no overt autophagy phenotype. When these floxed mice are bred to B6;129-Ulk1^tm1Kund/J mice (Stock No. 017976), and subsequently to mice that express Cre recombinase, double KO mice die within 24 hours of birth. When mouse embryonic fibroblasts (MEFs) are cultured from these double KO mice, amino-acid deprivation does not induce autophagy, while normal autophagy is evident during glucose deprivation and ammonia induction.

Development
A targeting vector was designed to insert a loxP site upstream of exon 1, and a loxP-flanked neomycin resistance (neo) cassette downstream of exon 3 of the Unc-51 like kinase 2 (Ulk2) gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl⁺-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric males were bred with C57BL/6J females. These mice were backcrossed to C57BL/6J mice for at least 10 generations. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Ulk2^tm1Thsn/Ulk2^tm1Thsn

        B6.129-Ulk2^tm1Thsn

• normal phenotype
• no abnormal phenotype detected
  • mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities   (MGI Ref ID J:181236)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cell Biology Research
Protein Processing
      degradation

Neurobiology Research
Cre-lox System
      loxP-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Ulk2tm1Thsn
Allele Name		targeted mutation 1, Craig B Thompson
Allele Type		Targeted (Floxed/Frt)
Common Name(s)		Ulk2FL;
Mutation Made By		Craig Thompson,   Memorial Sloan Kettering Cancer Center
Strain of Origin		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name		Ulk2, unc-51 like kinase 2
Chromosome		11
Gene Common Name(s)		A830085I22Rik; ATG1B; AU015340; RIKEN cDNA A830085I22 gene; Unc51.2; expressed sequence AU015340; mKIAA0623;
Molecular Note		A targeting vector was designed to insert a loxP site upstream of exon 1, and a loxP-flanked neomycin resistance (neo) cassette downstream of exon 3. [MGI Ref ID J:174297]

Genotyping

Genotyping Information

Genotyping Protocols

Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Neo Melt Curve Analysis,

Robotic MCA

Ulk2^tm1Thsn,

Separated MCA

Ulk2^tm1Thsn, Separated PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Cheong H; Lindsten T; Wu J; Lu C; Thompson CB. 2011. Ammonia-induced autophagy is independent of ULK1/ULK2 kinases. Proc Natl Acad Sci U S A 108(27):11121-6. [PubMed: 21690395]  [MGI Ref ID J:174297]

Additional References

Ulk2^tm1Thsn related

Thompson CB. 2012. Direct Data Submission 2012/03/13 MGI Direct Data Submission :.  [MGI Ref ID J:181236]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX18

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, homozygous mice may be bred together.
Mating System	Heterozygote x Heterozygote         (Female x Male)   21-JUN-12
Diet Information	LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering Information
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Terms of Use

Terms of Use

General Terms and Conditions

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Contact information

General inquiries regarding Terms of Use

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"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

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