Strain Name:

B6;129S4-Srsf1tm1Xdfu/J

Stock Number:

018020

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Availability:

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These floxed mutant mice possess loxP sites flanking exons 2-4 of the Srsf1 gene. This strain may be useful for generating conditional mutations in applications related to studies of embryonic retinal development.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
Generation?+F1pN1
Generation Definitions
 
Donating Investigator Xiang-Dong Fu,   University of California, San Diego

Description
Srsf1 (serine/arginine-rich splicing factor 1) is a serine/arginine (SR)-rich protein associated with the regulation of consititive splicing.

These targeted mutation mice carry a floxed allele of the gene. When crossed with a cre strain, tissue specific excision of exons 2-4 can be accomplished.

Ubiquitous excision of the floxed segment is early embryonic lethal. Conditional knockout in cardiomyocytes with a Myl2-cre strain causes postnatal contraction defects and dilated cardiomyopathy.

When bred to mice carrying Tg(Chx10-EGFP/cre,-ALPP)2Clc (see Stock No. 005105 for example) Cre recombinase expression in retina and Muller glial cells, this mutant mouse strain may be useful in studies of embryonic retinal development.

Development
A loxP site was placed in intron 1 and a loxP-flanked neomycin resistance cassette was introduced to intron 4 using 129S4/SvJae-derived J1 embryonic stem (ES)cells. Positive ES cell clones were transfected with a cre-expression plasmid to excise the floxed neomycin cassette. The strain was backcrossed to C57BL/6J one time by the donating laboratory.

Control Information

  Control
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
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Srsf1tm1Xdfu/Srsf1tm1Xdfu Tg(Chx10-EGFP/cre,-ALPP)2Clc/0

        involves: 129S4/SvJae * C57BL/6 * SJL   (conditional)
  • vision/eye phenotype
  • abnormal optic nerve morphology
    • thin optic nerve   (MGI Ref ID J:143205)
  • abnormal retina morphology
    • size of retina is decreased compared to wild-type   (MGI Ref ID J:143205)
    • retina is detached from retinal pigment epithelium (RPE), while abutting the lens   (MGI Ref ID J:143205)
    • eyes analyzed at P7 following BrdU injection at E16 show reduced cell numbers in outer nuclear layer (ONL), inner nuclear layer (INL), and ganglion cell layer (GCL)   (MGI Ref ID J:143205)
    • abnormal horizontal cell morphology
      • by P7, cell number is significantly reduced   (MGI Ref ID J:143205)
    • abnormal retina inner limiting membrane morphology
      • inner limiting membrane (ILM) structure is perturbed; retina has dysmorphic fibrous layer instead of well-organized ILM   (MGI Ref ID J:143205)
    • abnormal retinal ganglion layer morphology
      • ganglion cell layer (GCL) exhibits decreased cellularity in postnatal mice   (MGI Ref ID J:143205)
      • decreased retinal ganglion cell number
        • by P7, few ganglion cells are detected in retina   (MGI Ref ID J:143205)
    • decreased amacrine cell number
      • by P7, cell number is significantly reduced   (MGI Ref ID J:143205)
      • 2-fold decrease in BrdU-labeled cells is observed at P7 (BrdU injection at E16)   (MGI Ref ID J:143205)
    • retinal degeneration
      • by P14, rosettes are observed in retina   (MGI Ref ID J:143205)
      • ciliary body is fused to retina, so that it could not be separated from neural retina   (MGI Ref ID J:143205)
      • almost complete retinal degeneration has occurred by P30; entire eye is absent in mutants   (MGI Ref ID J:143205)
  • abnormal retinal apoptosis
    • at E12.5, 13.5, and 18.0, cell death is significantly elevated; increased apoptosis is observed primarily in central retina at E12.5 and 13.5 while periphery is affected at E18.0   (MGI Ref ID J:143205)
  • microphthalmia
    • newborn mice have small eyes   (MGI Ref ID J:143205)
    • total weight of eyes is 50% of wild-type   (MGI Ref ID J:143205)
  • nervous system phenotype
  • abnormal horizontal cell morphology
    • by P7, cell number is significantly reduced   (MGI Ref ID J:143205)
  • abnormal optic nerve morphology
    • thin optic nerve   (MGI Ref ID J:143205)
  • decreased amacrine cell number
    • by P7, cell number is significantly reduced   (MGI Ref ID J:143205)
    • 2-fold decrease in BrdU-labeled cells is observed at P7 (BrdU injection at E16)   (MGI Ref ID J:143205)
  • decreased retinal ganglion cell number
    • by P7, few ganglion cells are detected in retina   (MGI Ref ID J:143205)
  • cellular phenotype
  • abnormal retinal apoptosis
    • at E12.5, 13.5, and 18.0, cell death is significantly elevated; increased apoptosis is observed primarily in central retina at E12.5 and 13.5 while periphery is affected at E18.0   (MGI Ref ID J:143205)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Research Tools
Cardiovascular Research
      Cre-lox System
Cre-lox System
      loxP-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Srsf1tm1Xdfu
Allele Name targeted mutation 1, Xiang-Dong Fu
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) ASF/SF2II; Sfrs1fl;
Strain of Origin129S4/SvJae
Gene Symbol and Name Srsf1, serine/arginine-rich splicing factor 1
Chromosome 11
Gene Common Name(s) 1110054N12Rik; 5730507C05Rik; 6330415C05Rik; AI482334; ASF; AW491331; RIKEN cDNA 1110054N12 gene; RIKEN cDNA 5730507C05 gene; RIKEN cDNA 6330415C05 gene; SF2; SF2p33; SFRS1; SRp30a; Sfrs1; expressed sequence AI482334; expressed sequence AW491331; splicing factor, arginine/serine-rich 1 (ASF/SF2);
Molecular Note A loxP site was inserted upstream of exon 2 and a floxed neo-tk cassette was inserted downstream of exon 4. The neo-tk cassette was removed by transient expression of cre recombinase in correctly targeted ES cells. Resulting mutant animals carry loxP-flanked exons 2-4. [MGI Ref ID J:96039]

Genotyping

Genotyping Information

Genotyping Protocols

Srsf1tm1Xdfu, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Srsf1tm1Xdfu related

Kanadia RN; Clark VE; Punzo C; Trimarchi JM; Cepko CL. 2008. Temporal requirement of the alternative-splicing factor Sfrs1 for the survival of retinal neurons. Development 135(23):3923-33. [PubMed: 18987029]  [MGI Ref ID J:143205]

Xu X; Yang D; Ding JH; Wang W; Chu PH; Dalton ND; Wang HY; Bermingham JR Jr; Ye Z; Liu F; Rosenfeld MG; Manley JL; Ross J Jr; Chen J; Xiao RP; Cheng H; Fu XD. 2005. ASF/SF2-regulated CaMKIIdelta alternative splicing temporally reprograms excitation-contraction coupling in cardiac muscle. Cell 120(1):59-72. [PubMed: 15652482]  [MGI Ref ID J:96039]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryHomozygous or heterozygous mice may be bred to maintain a colony.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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