Description

Strain Information

Former Names C57BL/6-Cd101tm1.1Dil/J    (Changed: 20-SEP-12 ) Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Heterozygote x Heterozygote         (Female x Male)   18-OCT-12 Species laboratory mouse Generation +pN1 (26-NOV-12) Generation Definitions   Donating Investigator Linda Wicker,   University of Cambridge, UK

Description
The transmembrane CD101, also known as IGSF2 (Immunoglobulin superfamily, member 2) is expressed by regulatory T cells (Tregs), effector T cells, granulocytes, dendritic cells, and monocytes and is implicated in T cell proliferation inhibition. These mice carry a targeted mutation of the Cd101, CD101 antigen, gene. Homozygotes are viable, fertile and exhibit a lower granulocyte (Gr1+ cells in the bone marrow) number when compared to wildtype controls. No gene product (protein) is detected by FACS analysis of bone marrow and spleen cells. When challenged with Novosphingobium aromaticivorans exposure, homozygotes develop more severe liver disease than wildtype controls.

Development
A FRT site flanked Neo selection cassette followed by a loxP site and 3 stop codons was inserted downstream of exon 1 of the targeted gene, and another loxP site was inserted upstream of exon 1. This construct was electroporated into C57BL/6 derived Bruce4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to mice (on the C57BL/6 genetic background) expressing Cre recombinase(Oz-Cre deleter) to remove the exon 1 and the selection cassette. The mice were backcrossed to C57BL/6NTac for 7 generations using a marker assisted protocol. Heterozygotes were crossed to generate homozygotes. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6NJ (Stock No. 005304) at least once to establish the colony.

Control Information

	Control
	005304 C57BL/6NJ
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Cd101^tm1.1Dil/Cd101^tm1.1Dil

        involves: BALB/cJ * C57BL/6 * C57BL/6NTac

• hematopoietic system phenotype
• decreased granulocyte number
  • Gr-1+ cells in the bone marrow   (MGI Ref ID J:175943)
• immune system phenotype
• decreased granulocyte number
  • Gr-1+ cells in the bone marrow   (MGI Ref ID J:175943)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
Autoimmunity
Immunodeficiency

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Cd101tm1.1Dil
Allele Name		targeted mutation 1.1, Linda Wicker
Allele Type		Targeted (knock-out)
Mutation Made By		Linda Wicker,   University of Cambridge, UK
Strain of Origin		B6.Cg-Thy1
Gene Symbol and Name		Cd101, CD101 antigen
Chromosome		3
Gene Common Name(s)		EWI-101; Gm1016; Gm734; IGSF2; Igsf2; LOC381460; V7; gene model 1016, (NCBI); gene model 734, (NCBI); immunoglobulin superfamily, member 2;
Molecular Note		A loxP site was inserted upstream of exon 1. An FRT-flanked neo cassette with a 3' loxP site was inserted downstream of exon 1. Cre-mediated recombination removed exon 1 and the neo cassette. The absence of protein expression was confirmed by cell sorting on bone marrow and spleen cells. [MGI Ref ID J:175943]

Genotyping

Genotyping Information

Genotyping Protocols

Cd101^tm1.1Dil,

Separated MCA

Cd101^tm1.1Dil, Separated PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Rainbow DB; Moule C; Fraser HI; Clark J; Howlett SK; Burren O; Christensen M; Moody V; Steward CA; Mohammed JP; Fusakio ME; Masteller EL; Finger EB; Houchins JP; Naf D; Koentgen F; Ridgway WM; Todd JA; Bluestone JA; Peterson LB; Mattner J; Wicker LS. 2011. Evidence that Cd101 is an autoimmune diabetes gene in nonobese diabetic mice. J Immunol 187(1):325-36. [PubMed: 21613616]  [MGI Ref ID J:175943]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           FGB29

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice are bred as homozygotes.
Mating System	Heterozygote x Heterozygote         (Female x Male)   18-OCT-12
Diet Information	LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Control Information

	Control
	005304 C57BL/6NJ
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering Information
JAX^® Mice
Surgical and Preconditioning Services
JAX^® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.