Strain Name:

B6.129S1-Plcb2tm1Dwu/J

Stock Number:

018064

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Availability:

Repository- Live

Plcb2 (phospholipase C, beta 2) knockout mice show enhanced resistance to viral and bacterial infection, and lack sweet and bitter taste reception.

Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Additional information on Congenic nomenclature.
Mating SystemHomozygote x Homozygote         (Female x Male)   25-FEB-14
Specieslaboratory mouse
GenerationN4pN1+N1F4 (18-NOV-14)
Generation Definitions
 
Donating Investigator Melvin Simon,   University of California, San Diego

Description
Plcb2 (phospholipase C, beta 2) hydryolyzes phosphatidylinositol 4,5-bisphosphate to produce two second messengers, inositol triphosphates and diacylglycerol. Expressed in hematopoietic cells and taste tissues, it is involved with chemoattractant-mediated signal transduction.

Knockout mice do not show any defects in hematopoiesis, but chemoattractant-induced Ca2+ release and superoxide production in neutrophils is blocked. Chemotaxis of different leukocyte populations is enhanced. Immune responses to bacteria and viruses are not impaired and may even be enhanced.

Homozygotes lack sweet, amino acid, and bitter taste reception, but sour and salty taste perception are not affected.

These mice may still be segregating for the alleles present in the embryonic stem cells, including coat color alleles.

Development
A portion of two exons encoding residues 378-464 (located in the C terminus of the Xbox) were replaced with a neomycin resistance gene. The mutation was created in 129S1/Sv-p+ Tyr+ Kitl+-derived CJ7 embryonic stem (ES) cells. The donating laboratory reports mice were backcrossed four times to C57BL/6 (see SNP results below) prior to sending agouti males to The Jackson Laboratory Repository in 2012. Upon arrival, mutant males were bred to C57BL/6J females (Stock No. 000664), resulting in four mutant offspring (two females and two males). The two males (one agouti and one black) were used to cryopreserve sperm. The two females (both agouti) were bred to C57BL/6J males to establish the living mouse colony. Thereafter, mutant females were bred with mutant males to maintain the living colony. These mice may still be segregating for the alleles present in the embryonic stem cells, including coat color alleles.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the first generation female mice at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be heterozygous. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.

Control Information

  Control
   000664 C57BL/6J (approximate)
   005304 C57BL/6NJ (approximate)
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Plcb2tm1Dwu/Plcb2tm1Dwu

        involves: 129/Sv
  • immune system phenotype
  • abnormal leukocyte physiology
    • chemotaxic responses of leukocytes are enhanced   (MGI Ref ID J:41948)
  • decreased susceptibility to infection
    • enhanced resistance to both bacterial and viral infection   (MGI Ref ID J:41948)
    • decreased susceptibility to bacterial infection   (MGI Ref ID J:41948)
    • decreased susceptibility to viral infection   (MGI Ref ID J:41948)
  • hematopoietic system phenotype
  • abnormal leukocyte physiology
    • chemotaxic responses of leukocytes are enhanced   (MGI Ref ID J:41948)

Plcb2tm1Dwu/Plcb2tm1Dwu

        involves: 129/Sv * SWR
  • taste/olfaction phenotype
  • abnormal gustatory system physiology
    • nerve responses to sweet, amino acid, and bitter stimuli were abolished   (MGI Ref ID J:82314)
    • normal nerve responses to salty or sour stimuli   (MGI Ref ID J:82314)
    • abnormal taste sensitivity
      • preference for sweet, amino acid or bitter vs. water abolished   (MGI Ref ID J:82314)
      • preference for salty or sour vs. water similar to controls   (MGI Ref ID J:82314)
      • abnormal bitter taste sensitivity   (MGI Ref ID J:82314)
      • abnormal sweet taste sensitivity   (MGI Ref ID J:82314)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cell Biology Research
Signal Transduction

Immunology, Inflammation and Autoimmunity Research
Intracellular Signaling Molecules

Sensorineural Research
Taste Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Plcb2tm1Dwu
Allele Name targeted mutation 1, Dianqing Wu
Allele Type Targeted (Null/Knockout)
Mutation Made By Dianqing Wu,   Yale University School of Medicine
Strain of Origin129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
Gene Symbol and Name Plcb2, phospholipase C, beta 2
Chromosome 2
Gene Common Name(s) AI550384; B230205M18Rik; PLC-beta-2; RIKEN cDNA B230205M18 gene; expressed sequence AI550384;
Molecular Note Sequence encoding the X box was disrupted by the insertion of a neomycin selection cassette. Western blot analysis of spleen and thymus extracts showed an absence of protein. [MGI Ref ID J:41948]

Genotyping

Genotyping Information

Genotyping Protocols

Plcb2tm1Dwu, High Resolution Melting
Plcb2tm1Dwu, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Jiang H; Kuang Y; Wu Y; Xie W; Simon MI; Wu D. 1997. Roles of phospholipase C beta2 in chemoattractant-elicited responses. Proc Natl Acad Sci U S A 94(15):7971-5. [PubMed: 9223297]  [MGI Ref ID J:41948]

Additional References

Plcb2tm1Dwu related

Grinberg S; Hasko G; Wu D; Leibovich SJ. 2009. Suppression of PLCbeta2 by endotoxin plays a role in the adenosine A(2A) receptor-mediated switch of macrophages from an inflammatory to an angiogenic phenotype. Am J Pathol 175(6):2439-53. [PubMed: 19850892]  [MGI Ref ID J:155351]

Li Z; Jiang H; Xie W; Zhang Z; Smrcka AV; Wu D. 2000. Roles of PLC-beta2 and -beta3 and PI3Kgamma in chemoattractant-mediated signal transduction [see comments] Science 287(5455):1046-9. [PubMed: 10669417]  [MGI Ref ID J:60348]

Lian L; Wang Y; Draznin J; Eslin D; Bennett JS; Poncz M; Wu D; Abrams CS. 2005. The relative role of PLCbeta and PI3Kgamma in platelet activation. Blood 106(1):110-7. [PubMed: 15705797]  [MGI Ref ID J:107460]

Mueller KL; Hoon MA; Erlenbach I; Chandrashekar J; Zuker CS; Ryba NJ. 2005. The receptors and coding logic for bitter taste. Nature 434(7030):225-9. [PubMed: 15759003]  [MGI Ref ID J:97593]

Suire S; Lecureuil C; Anderson KE; Damoulakis G; Niewczas I; Davidson K; Guillou H; Pan D; Clark J; Hawkins PT; Stephens L. 2012. GPCR activation of Ras and PI3Kgamma in neutrophils depends on PLCbeta2/beta3 and the RasGEF RasGRP4. EMBO J :. [PubMed: 22728827]  [MGI Ref ID J:186412]

Wang Z; Liu B; Wang P; Dong X; Fernandez-Hernando C; Li Z; Hla T; Li Z; Claffey K; Smith JD; Wu D. 2008. Phospholipase C beta3 deficiency leads to macrophage hypersensitivity to apoptotic induction and reduction of atherosclerosis in mice. J Clin Invest 118(1):195-204. [PubMed: 18079968]  [MGI Ref ID J:130826]

Xiao W; Hong H; Kawakami Y; Kato Y; Wu D; Yasudo H; Kimura A; Kubagawa H; Bertoli LF; Davis RS; Chau LA; Madrenas J; Hsia CC; Xenocostas A; Kipps TJ; Hennighausen L; Iwama A; Nakauchi H; Kawakami T. 2009. Tumor suppression by phospholipase C-beta3 via SHP-1-mediated dephosphorylation of Stat5. Cancer Cell 16(2):161-71. [PubMed: 19647226]  [MGI Ref ID J:151972]

Zhang Y; Hoon MA; Chandrashekar J; Mueller KL; Cook B; Wu D; Zuker CS; Ryba NJ. 2003. Coding of sweet, bitter, and umami tastes: different receptor cells sharing similar signaling pathways. Cell 112(3):293-301. [PubMed: 12581520]  [MGI Ref ID J:82314]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           FGB27

Colony Maintenance

Breeding & HusbandryHeterozygotes and homozygotes are viable and fertile.
Mating SystemHomozygote x Homozygote         (Female x Male)   25-FEB-14
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $199.90Female or MaleHomozygous for Plcb2tm1Dwu  
Price per Pair (US dollars $)Pair Genotype
$399.80Homozygous for Plcb2tm1Dwu x Homozygous for Plcb2tm1Dwu  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $259.90Female or MaleHomozygous for Plcb2tm1Dwu  
Price per Pair (US dollars $)Pair Genotype
$519.80Homozygous for Plcb2tm1Dwu x Homozygous for Plcb2tm1Dwu  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   000664 C57BL/6J (approximate)
   005304 C57BL/6NJ (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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