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| This tri-allelic Prism JD1849 mouse model allows distinct fluorescent visualization of neurons, astrocytes, and oligodendrocytes and may be useful for noninvasive and/or in vivo studies of the central nervous system and neural circuits, as well as a source for cell culture experiments. | |||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Former Names FVB-Tg(Snap25-mcherry,Mobp-YFP/HA,Aldh1l1-Cerulean/Myc)1849Htz/J (Changed: 23-MAY-12 ) Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Hemizygote x Inbred (Female x Male) 30-JUL-12 Species laboratory mouse Generation ?pN1+N1 (29-OCT-12)
Generation DefinitionsDonating Investigator Nathaniel Heintz, The Rockefeller University/HHMI Description
These transgenic mice express three distinct fluorophores in subsets of cells in the brain: HA tagged YFP under the control of Mobp for yellow fluorescing oligodendrocytes, a Myc tagged Cerulean (CFP) under the control of Aldh1l1 for bluegreen fluorescing astrocytes, and mCherry under the control of Snap25 for red fluorescing neurons. Transgenic mice exhibit a head rearing behavior as early as postnatal day 21, and have impaired locomotor abilities and other behavioral abnormalities. Male hemizygotes do not breed due to the behavioral phenotype. Female hemizygotes make poor mothers. Transgene expression, for each transgenic construct, mimics endogenous gene expression patterns. Cerulean fluorescence is dimmer than YFP and mCherry. mCherry expression increases with age of the mouse. FISH analysis of cultured astrocytes shows all three transgenes integrated into a single locus on chromosome 11. The Donating Investigator has not attempted to make the strain homozygous.Development
Three mouse bacterial artificial chromosome (BAC) transgenic constructs were co-injected to generate this Prism 1849 strain. The BAC RP23-290A18, containing the entire Snap25 gene, was modified by inserting mCherry into the translation start site (ATG) of Snap25. The BAC RP23-172H7, containing the entire Mobp gene, was modified by inserting a HA tagged YFP into the translation start site (ATG) of Mobp. The BAC RP23-7M9, containing the entire Aldh1l1 gene, was modified by inserting a Myc tagged Cerulean (CFP) into the translation start site (ATG) of Aldh1l1. The 3 transgenic constructs were then co-injected into FVB fertilized mouse eggs. Founder line 1849 was subsequently established. FISH of cultured astrocytes shows all three transgenes integrated into a single locus on chromosome 11. Upon arrival at The Jackson Laboratory, the mice were crossed to FVB/NJ (Stock No. 001800) at least once to establish the colony.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Aldh1l1
018068 FVB-Tg(Prism)1989Htz/J View Strains carrying other alleles of Aldh1l1 (1 strain)
004218 B6.129(ICR)-Tg(CAG-ECFP)CK6Nagy/J 008513 B6.129P2-Gt(ROSA)26Sortm1(Trpv1,ECFP)Mde/J 007901 B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J 007911 B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J 007921 B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J 003710 B6.Cg-Tg(Thy1-CFP)23Jrs/J 014131 B6.Cg-Tg(Thy1-CFP)IJrs/GfngJ 007940 B6.Cg-Tg(Thy1-CFP/COX8A)C1Lich/J 007967 B6.Cg-Tg(Thy1-CFP/COX8A)S2Lich/J 013161 B6.Cg-Tg(Thy1-Clomeleon)1Gjau/J 007910 B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J 008004 B6;SJL-Tg(Thy1-ECFP/VAMP2)1Sud/J 007856 B6SJL-Tg(Thy1-Syt1/ECFP)1Sud/J 018671 D2.Cg-Tg(Thy1-CFP)23Jrs/SjJ 018068 FVB-Tg(Prism)1989Htz/J 007879 STOCK Stx1atm2Sud/J 003773 STOCK Tg(CAG-ECFP)CK6Nagy/J 006784 STOCK Tg(Ins1-Cerulean)24Hara/J 016922 STOCK Tg(Myh7-CFP)1Jrs/J 013162 STOCK Tg(Thy1-Clomeleon)12Gjau/J 013163 STOCK Tg(Thy1-Clomeleon)13Gjau/J
018071 FVB-Tg(Prism)1861Htz/J 018068 FVB-Tg(Prism)1989Htz/J
004863 B6.129X1-Snap25tm1Mcw/J 021879 B6.Cg-Snap25tm1.1Hze/J 018071 FVB-Tg(Prism)1861Htz/J 018068 FVB-Tg(Prism)1989Htz/J
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
YFP relatedNeurobiology Research
Fluorescent protein expression in neural tissue
Research Tools
Fluorescent Proteins
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Prism)1849Htz | ||
|---|---|---|---|
| Allele Name | transgene insertion 1849, Nathaniel Heintz | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | Prism JD1849; Snap25::mcherry, Mobp::Yfp-HA, Aldh1L1::Cerulean-Myc; Tg(Snap25-mcherry,Mobp-YFP,Aldh1l1-Cerulean)1849Htz; | ||
| Strain of Origin | FVB | ||
| Site of Expression | HA tagged YFP is expressed in oligodendrocytes, Myc tagged CFP is expressed in astrocytes, and mCherry is expressed in neurons. | ||
| Expressed Gene | CFP, Cyan Fluorescent Protein, jellyfish | ||
| Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish | ||
| Yellow Fluorescent Protein (YFP) is a derivative of Green Fluorescent Protein (GFP), a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance fluorescence intensity and shift the wavelength emitted when excited. When YFP is utilized in a transgenic construct, tissue expressing sufficient amounts of YFP will fluoresce yellowish-green when exposed to a 513 nm light source. | |||
| Expressed Gene | RFP, Red Fluorescent Protein, coral | ||
| Red Fluorescent Protein (RFP), derived from marine invertebrate organisms such as the soft coral Discosoma spp and reef coral, Heteractis crispa, is a versatile reporter molecule which has found use in many biological applications. The wild type protein, which is an obligate tetramer, is not well tolerated in mammalian systems. The original molecule has been modified in order to optimize expression to mammalian physiology (examples include monomeric RFP, mRFP1, DsRed, etc). | |||
| Promoter | Aldh1l1, aldehyde dehydrogenase 1 family, member L1, mouse, laboratory | ||
| Promoter | Mobp, myelin-associated oligodendrocytic basic protein, mouse, laboratory | ||
| Promoter | Snap25, synaptosomal-associated protein 25, mouse, laboratory | ||
| Molecular Note | Three mouse bacterial artificial chromosome (BAC) transgenic constructs were co-injected to generate this Prism 1849 strain. The BAC RP23-290A18, containing the entire Snap25 gene, was modified by inserting mCherry into the translation start site (ATG) of Snap25. The BAC RP23-172H7, containing the entire Mobp gene, was modified by inserting a HA tagged YFP into the translation start site (ATG) of Mobp. The BAC RP23-7M9, containing the entire Aldh1l1 gene, was modified by inserting a Myc tagged Cerulean (CFP) into the translation start site (ATG) of Aldh1l1. Founder line 1849 was subsequently established. FISH of cultured astrocytes shows all three transgenes integrated into asingle locus on chromosome 11. [MGI Ref ID J:189629] | ||
| Gene Symbol and Name | Tg(Prism)1849Htz, transgene insertion 1849, Nathaniel Heintz | ||
| Chromosome | UN | ||
Genotyping
Genotyping Information
Genotyping Protocols
Cerulean,Separated MCA
Generic mCherry, Separated PCR
Generic mCherry, Standard PCR
Cerulean, Separated PCR
Fluorescent Proteins (Generic GFP), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Dougherty JD; Zhang J; Feng H; Gong S; Heintz N. 2012. Mouse transgenesis in a single locus with independent regulation for multiple fluorophores. PLoS One 7(7):e40511. [PubMed: 22808177] [MGI Ref ID J:189629]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number FGB29
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, female hemizygotes may be bred with wildtype (noncarrier) mice from the colony or FVB/NJ inbred mice (Stock No. 001800). Foster mothers are recommended as female hemizygotes do not make good mothers. Male hemizygotes do not breed due to a behavioral phenotype. Mating System Hemizygote x Inbred (Female x Male) 30-JUL-12
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Hemizygous for Tg(Prism)1849Htz
Price per Pair (US dollars $) Pair Genotype $296.00 Hemizygous for Tg(Prism)1849Htz x Noncarrier Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
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Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Hemizygous for Tg(Prism)1849Htz
Price per Pair (US dollars $) Pair Genotype $384.80 Hemizygous for Tg(Prism)1849Htz x Noncarrier Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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