Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Gene Trap; Mutant Strain; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N?pN1 Generation Definitions   Donating Investigator Huda Zoghbi,   Baylor College of Medicine

Description
Cic (capicua homolog (Drosophila)) is a transcriptional repressor gene regulated by Egf (epidermal growth factor).

Most homozygous Cic gene-trap mice die around postnatal day 21 (P21). Those that survive are smaller than their littermates and show a lung alveolarization defect. They lack expression of the Cic-L (long) isoform, but express reduced levels (~15%) of Cic-S (short) isoform in the lung and cerebellum. As such, this allele is considered to be a hypomorph.

Heterozygotes express both the Cic-L and Cic-S isoforms at about 50% normal levels. Disruption of the Cic gene is accompanied by a proportionate reduction in the expression of Atxn1 (ataxin 1) and Atxn1l (ataxin 1-like) in the cerebellum, confirming the interdependency of the genes. Mutations in Atxn1 are associated with spinocerebellar ataxia type I (SCA1).

Development
Bay Genomics gene trap ES cell line XE565, generated in 129P2/Ola-derived E14Tg2a.4 embryonic stem cells, was used to produce these mice. The gene trap vector contained a splice-acceptor sequence upstream of beta-geo (a fusion of beta-galactosidase and neomycin phosphotransferase II), and was inserted into an intronic or coding region of genomic DNA just 3' of the Cic-L isoform. This created a fusion transcript containing sequences from exons upstream of the insertion joined to the beta-geo marker, disrupting the gene. No Cic-L isoform is produced and levels of Cic-S protein are 90% reduced. The donating laboratory backcrossed this line to C57BL/6 for 10 generations.

Control Information

	Control
	Wild-type from the colony
	000664 C57BL/6J
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Cic^Gt(XE565)Byg/Cic⁺

        B6.129P2-Cic^Gt(XE565)Byg

• mortality/aging
• *normal* mortality/aging
  • mice exhibit normal survival   (MGI Ref ID J:177841)
• behavior/neurological phenotype
• *normal* behavior/neurological phenotype
  • mice exhibit normal activity, motor coordination, and fear conditioning   (MGI Ref ID J:177841)

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Cic^Gt(XE565)Byg/Cic^Gt(XE565)Byg

        involves: 129P2/OlaHsd * C57BL/6J

• mortality/aging
• partial postnatal lethality
  • most die before weaning   (MGI Ref ID J:178303)
• growth/size phenotype
• decreased body size
  • some survivors are smaller than wild type littermates   (MGI Ref ID J:178303)
• respiratory system phenotype
• abnormal pulmonary alveolus morphology
  • severe alveolarization defects   (MGI Ref ID J:178303)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cell Biology Research
Transcriptional Regulation

Developmental Biology Research
Growth Defects
      Growth Defects (homozygous)
Internal/Organ Defects
      lung
Postnatal Lethality
      Homozygous

Internal/Organ Research
Lung Defects

Neurobiology Research
Ataxia (Movement) Defects
      Spinocerebellar ataxia

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		CicGt(XE565)Byg
Allele Name		gene trap XE565, BayGenomics
Allele Type		Gene trapped
Common Name(s)		Cic-L-;
Mutation Made By		Huda Zoghbi,   Baylor College of Medicine
Strain of Origin		129P2/OlaHsd
Gene Symbol and Name		Cic, capicua homolog (Drosophila)
Chromosome		7
Gene Common Name(s)		1200010B10Rik; RIKEN cDNA 1200010B10 gene; mKIAA0306;
Molecular Note		The gene trap vector inserted downstream of exon 1. The absence of protein expression of the long isoform was confirmed by western blot anaylsis. The expression of the short isoform is reduced to 10% of wild-type. [MGI Ref ID J:177841]

Genotyping

Genotyping Information

Genotyping Protocols

Cic^Gt(XE565)Bygalternate1 MCA,

Separated MCA

Cic^Gt(XE565)Bygalternate1, Separated PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Fryer JD; Yu P; Kang H; Mandel-Brehm C; Carter AN; Crespo-Barreto J; Gao Y; Flora A; Shaw C; Orr HT; Zoghbi HY. 2011. Exercise and genetic rescue of SCA1 via the transcriptional repressor Capicua. Science 334(6056):690-3. [PubMed: 22053053]  [MGI Ref ID J:177841]

Additional References

Cic^Gt(XE565)Byg related

Lee Y; Fryer JD; Kang H; Crespo-Barreto J; Bowman AB; Gao Y; Kahle JJ; Hong JS; Kheradmand F; Orr HT; Finegold MJ; Zoghbi HY. 2011. ATXN1 protein family and CIC regulate extracellular matrix remodeling and lung alveolarization. Dev Cell 21(4):746-57. [PubMed: 22014525]  [MGI Ref ID J:178303]

Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI). 2008. Mouse Gene Trap Data Load from dbGSS Database Download :.  [MGI Ref ID J:141210]

Stryke D; Kawamoto M; Huang CC; Johns SJ; King LA; Harper CA; Meng EC; Lee RE; Yee A; L'Italien L; Chuang PT; Young SG; Skarnes WC; Babbitt PC; Ferrin TE. 2003. BayGenomics: a resource of insertional mutations in mouse embryonic stem cells. Nucleic Acids Res 31(1):278-81. [PubMed: 12520002]  [MGI Ref ID J:91812]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & Husbandry	Heterozygotes are viable and fertile. Most homozygotes die around postnatal day 21 (P21)

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Control Information

	Control
	Wild-type from the colony
	000664 C57BL/6J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering Information
JAX^® Mice
Surgical and Preconditioning Services
JAX^® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.