Description

Strain Information

Former Names B6.Cg-Mir34atm1Lhe/J    (Changed: 08-MAY-12 ) Type Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote         (Female x Male)   31-MAY-13 Species laboratory mouse   Donating Investigator Lin He,   UC-Berkeley

Description
The miR-34 family of miRNAs (miR-34a, miR-34b, and miR-34c) are expressed mainly in the testis, brain, and lung. miR-34 proteins are induced upon p53 activation and are associated with tumor suppression, aging, neurodegeneration, and spermatogenesis. In this strain, a targeting vector including Kozak sequence, lacZ and a neomycin cassette was used to disrupt Mir34a. No gene product (mRNA) is detected in MEFs (mouse embryo fibroblasts) from homozygotes by RT-PCR analysis. Homozygous Mir34a^-/- mice are viable and fertile. MEFs from Mir34a^-/- mice exhibit increased reprogramming efficiency and induction of iPSCs (induced pluripotent stem cell) when compared to controls. Mutant mice may have applications in studies related to somatic cell reprogramming and regulatory control of p53 pathways.

Development
A targeting vector containing Kozak sequence, the lacZ gene and a FRT & loxP site flanked NEO cassette, was used to disrupt Mir34a and 20 bp flanking sequence. The construct was electroporated into C57BL/6-derived Bruce 4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Mir34a

018545

STOCK Mir34atm1.2Aven/J

View Strains carrying other alleles of Mir34a     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Mir34a^tm1Lhe/Mir34a⁺

        involves: C57BL/6

• no phenotypic analysis
• *normal* no phenotypic analysis   (MGI Ref ID J:180692)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cell Biology Research
Cell Cycle Regulation
Genes Regulating Growth and Proliferation

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Mir34atm1Lhe
Allele Name		targeted mutation 1, Lin He
Allele Type		Targeted (Floxed/Frt)
Common Name(s)		Mir34adelta;
Strain of Origin		B6.Cg-Thy1
Gene Symbol and Name		Mir34a, microRNA 34a
Chromosome		4
Gene Common Name(s)		Mirn34a; mir 34a; mmu-mir-34a;
Molecular Note		A targeting vector containing Kozak sequence, the lacZ gene and a FRT and loxP site flanked NEO cassette, was used to disrupt the pre-microRNA and 20 bp flanking sequence. [MGI Ref ID J:180692]

Genotyping

Genotyping Information

Genotyping Protocols

Mir34a^tm1Lhealternate 1,

Separated MCA

Mir34a^tm1Lhealternate1, Separated PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Choi YJ; Lin CP; Ho JJ; He X; Okada N; Bu P; Zhong Y; Kim SY; Bennett MJ; Chen C; Ozturk A; Hicks GG; Hannon GJ; He L. 2011. miR-34 miRNAs provide a barrier for somatic cell reprogramming. Nat Cell Biol 13(11):1353-60. [PubMed: 22020437]  [MGI Ref ID J:180692]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as homozygotes.
Mating System	Homozygote x Homozygote         (Female x Male)   31-MAY-13

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

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See Terms of Use tab for General Terms and Conditions

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Terms of Use

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