Strain Name:

B6;SJL-Agttm1.1Itl/J

Stock Number:

018388

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Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
This Agtfl strain is useful when studying the control of blood pressure by the rennin-angiotensin system.

Description

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Mating SystemHomozygote x Homozygote         (Female x Male)   04-APR-14
Specieslaboratory mouse
GenerationN3pN1+ (10-AUG-13)
Generation Definitions
 
Donating Investigator Lisa A Cassis,   University of Kentucky

Description
These Agtfl mutant mice possess loxP sites flanking exon 2 of the angiotensinogen (Agt) gene. AGT is a circulating α-2-globulin produced mainly by the liver. It is the only known precursor to the peptide angiotensin II (ANG II), which is a hormone linked to hypertension. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues.

For example when bred to B6.Cg-Tg(Fabp4-cre)1Rev/J mice (Stock No. 005069), AGT depletion in adipocytes results in decreased systolic blood pressure and reduced circulating AGT concentrations.

Development
A targeting vector was designed to insert a loxP site upstream of exon 2 and a floxed and frt-flanked neomycin resistance (neo) cassette downstream of exon 2 of the angiotensinogen (Agt) gene. The construct was electroporated into C57BL/6NTac-derived iTL C57/Bl6 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric mice were bred together. The resulting offspring were bred with B6;SJL-Tg(ACTFLPe)9205Dym/J transgenic mice (Stock No. 003800) to delete the neo cassette, and progeny were crossed to remove the Flp-expressing transgene. These Agtfl mice were bred to C57BL/6 mice for at least 3 generations. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Control Information

  Control
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Agt
002681   B6.129P2-Agttm1Unc/J
002690   B6.129P2-Agttm2Unc/J
View Strains carrying other alleles of Agt     (2 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Angiotensinogen; AGT   (AGT)
Hypertension, Essential   (AGT)
Renal Tubular Dysgenesis; RTD   (AGT)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Agttm1.1Itl/Agttm1.1Itl Tg(Fabp4-cre)1Rev/0

        involves: C57BL/6 * C57BL/6J * C57BL/6NTac * SJL   (conditional)
  • homeostasis/metabolism phenotype
  • *normal* homeostasis/metabolism phenotype
    • no significant difference in glucose homeostasis compared to controls   (MGI Ref ID J:178836)
    • decreased circulating angiotensinogen level
      • in males and females at 2 and 12 months of age compared to control littermates   (MGI Ref ID J:178836)
  • cardiovascular system phenotype
  • decreased systemic arterial systolic blood pressure
    • in males and females at 2 and 12 months of age compared to control littermates   (MGI Ref ID J:178836)
  • adipose tissue phenotype
  • *normal* adipose tissue phenotype
    • no significant difference in fat mass or adipocyte size compared to controls   (MGI Ref ID J:178836)
  • growth/size/body phenotype
  • *normal* growth/size/body phenotype
    • no significant difference in body weight compared to controls   (MGI Ref ID J:178836)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cardiovascular Research
Hypertension

Research Tools
Cre-lox System
      loxP-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Agttm1.1Itl
Allele Name targeted mutation 1.1, inGenious Targeting Laboratory
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) Agtfl;
Strain of OriginC57BL/6NTac
Gene Symbol and Name Agt, angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
Chromosome 8
Gene Common Name(s) AI265500; ANHU; ANRT; Ang; AngII; Aogen; PAT; SERPINA8; angiotensin precursor; expressed sequence AI265500;
Molecular Note A loxP site was inserted upstream of exon 2 and an FRT and loxP flanked neo cassette were inserted downstream of exon 2 via homologous recombination. Flp mediated recombination deleted the neo cassette leaving exon 2 floxed. [MGI Ref ID J:178836]

Genotyping

Genotyping Information

Genotyping Protocols

Agttm1.1Itl, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Batifoulier-Yiannikouris F; Karounos M; Charnigo R; English VL; Rateri DL; Daugherty A; Cassis LA. 2011. Adipocyte-specific deficiency of angiotensinogen decreases plasma angiotensinogen concentration and systolic blood pressure in mice. Am J Physiol Regul Integr Comp Physiol :. [PubMed: 22071160]  [MGI Ref ID J:178836]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX18

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice may be bred together.
Mating SystemHomozygote x Homozygote         (Female x Male)   04-APR-14

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHomozygous for Agttm1.1Itl  
Price per Pair (US dollars $)Pair Genotype
$464.00Homozygous for Agttm1.1Itl x Homozygous for Agttm1.1Itl  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHomozygous for Agttm1.1Itl  
Price per Pair (US dollars $)Pair Genotype
$603.20Homozygous for Agttm1.1Itl x Homozygous for Agttm1.1Itl  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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