Strain Name:

B6.129X1(Cg)-Bcl2tm1.1Sjk/J

Stock Number:

018430

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Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
B cell leukemia/lymphoma 2 (BCL2) is an anti-apoptotic, anti-autophagy protein that inhibits autophagy by interacting with Beclin 1 (BECN1). Alanine substitutions in these mice prevent stimulus-induced disruption of the BCL2-BECN1 complex and autophagy activation. These mice may be useful in studies of induced autophagy, metabolism, and exercise.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
GenerationN10pN1
Generation Definitions
 
Donating Investigator Beth Levine,   University of Texas Southwestern Medical

Description
B cell leukemia/lymphoma 2 (BCL2) is an anti-apoptotic, anti-autophagy protein that inhibits autophagy by interacting with Beclin 1 (BECN1). Disruption of the BCL2-BECN1 complex is necessary for stimulus (exercise or starvation)- induced autophagy. Bcl2AAA mice carry alanine substitutions in three conserved phosphorylation sites (T69A, S70A, S84A) that prevent stimulus-induced disruption of the BCL2-BECN1 complex and autophagy activation. Homozygous mice are viable and fertile. During acute exercise, mice exhibit decreased endurance, impaired insulin sensitivity, altered glucose metabolism and impaired protection against glucose intolerance from high fat diet. Bcl2AAA mice may be useful in studies of induced autophagy, metabolism, and exercise.

Development
A targeting vector containing an frt-flanked puromycin resistance cassette was used to insert alanine substitutions into amino acid residues 69 (threonine), 70 (serine), and 84 (serine) of the Bcl2 gene (Bcl2AAA). The alanine substitutions were directed to three conserved phosphorylation sites of the exon 2 loop region. The construct was electroporated into 129X1/SvJ-derived RW4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric mice were bred with B6;SJL-Tg(ACTFLPe)9205Dym/J to remove the puro cassette. Mice were backcrossed to C57BL/6J for more than 10 generations.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Bcl2
003082   129S1/SvImJ-Bcl2tm1Mpin/J
003069   B6.NOD-(D1Mit3-Bcl2) (D17Mit21-D17Mit10)/LtJ
003062   B6.NOD-(D1Mit3-Bcl2)/J
002265   B6;129S2-Bcl2tm1Sjk/J
008882   STOCK Bcl2tm1Irt/J
View Strains carrying other alleles of Bcl2     (5 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
B-Cell Cll/Lymphoma 2; BCL2   (BCL2)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Diabetes and Obesity Research
Insulin Resistance

Metabolism Research

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Bcl2tm1.1Sjk
Allele Name targeted mutation 1.1, Stanley Korsmeyer
Allele Type Targeted
Common Name(s) Bcl2AAA; Bcl2tm1.1Blev;
Mutation Made By Beth Levine,   University of Texas Southwestern Medical
Strain of Origin129X1/SvJ
Promoter Bcl2, B cell leukemia/lymphoma 2, mouse, laboratory
Molecular Note Nucleotide substitutions in exon 2 were used to replace the threonine at 69 and serines at positions 70 and 84 with alanines (T69A, S70A and S84A). Flp-mediated recombination removes the puro cassette inserted downstream of the modified exon 2. [MGI Ref ID J:181308]

Genotyping

Genotyping Information

Genotyping Protocols

Bcl2tm1.1SjkPyro, Pyrosequencing


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Bcl2tm1.1Sjk related

He C; Bassik MC; Moresi V; Sun K; Wei Y; Zou Z; An Z; Loh J; Fisher J; Sun Q; Korsmeyer S; Packer M; May HI; Hill JA; Virgin HW; Gilpin C; Xiao G; Bassel-Duby R; Scherer PE; Levine B. 2012. Exercise-induced BCL2-regulated autophagy is required for muscle glucose homeostasis. Nature 481(7382):511-5. [PubMed: 22258505]  [MGI Ref ID J:181308]

He C; Sumpter R Jr; Levine B. 2012. Exercise induces autophagy in peripheral tissues and in the brain. Autophagy 8(10):1548-51. [PubMed: 22892563]  [MGI Ref ID J:192952]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhile maintaining a live colony, these mice are bred as homozygotes.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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