Strain Name:


Stock Number:



Awaiting Transfer from the Donor

On 16-APR-13 this strain was accepted for import and is now awaiting transfer to our campus.
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In these p53LSL-53,54 mice, which carry a mutation in the 2nd transcriptional activation domain, expression of the mutant TRP53 protein is silenced until Cre recombinase mediates excision of the floxed STOP cassette. These mice may be useful in studies of p53 function and the role of p53 as a transcriptional activator in tumor suppression.


Strain Information

Type Coisogenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
Donating Investigator Laura Attardi,   Stanford University Medical Center

The Trp53, transformation related protein 53, gene encodes a tumor suppressor protein, which functions as a transcription factor regulating expression of more than 100 target genes. Mutations resulting in inactivation of p53 protein facilitates tumor progression. These p53LSL-53,54 mice carry F53Q;F54S point mutations in exon 4, in the 2nd transcriptional activation domain, and a floxed STOP cassette upstream of exon 2. Expression of the mutant TRP53 protein is silenced until Cre recombinase mediates excision of the floxed STOP cassette. After Cre recombinase mediated removal of the floxed STOP cassette cells from the resulting mice exhibit no compromise in transactivation of p53 targets relative to wildtype p53. When bred to Cre recombinase expressing mice, these mice could be used to generate tumor cells for engraftment experiments. Mice that are homozygous for the targeted mutation are viable, but have decreased fertility.

A targeting vector designed by Dr. Laura D. Attardi (Stanford University School of Medicine) containing a floxed STOP element with 4 poly adenylation sites, and a puromycin resistance cassette, was utilized to insert the floxed STOP cassette upstream of exon 2 and the F53Q;F54S point mutation into exon 4. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to 129 Sv/J mice. Upon arrival at The Jackson Laboratory, the mice were crossed to 129S1/SvImJ (Stock No. 002448) at least once to establish the colony.

Control Information

   Heterozygote from the colony
   Wild-type from the colony
   000664 C57BL/6J (approximate)
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of Trp53     (27 strains)


Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Adrenocortical Carcinoma, Hereditary; ADCC   (TP53)
Basal Cell Carcinoma, Susceptibility to, 7; BCC7   (TP53)
Breast Cancer   (TP53)
Colorectal Cancer; CRC   (TP53)
Glioma Susceptibility 1; GLM1   (TP53)
Hepatocellular Carcinoma   (TP53)
Li-Fraumeni Syndrome 1; LFS1   (TP53)
Nasopharyngeal Carcinoma   (TP53)
Osteogenic Sarcoma   (TP53)
Pancreatic Cancer   (TP53)
Papilloma of Choroid Plexus; CPP   (TP53)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype


        involves: 129S4/SvJae   (conditional)
  • cellular phenotype
  • *normal* cellular phenotype
    • ell cycle is arrested by gamma irradiation after treatment with an adenovirus-cre as it is in controls   (MGI Ref ID J:173395)

The following phenotype relates to a compound genotype created using this strain.
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Trp53tm3Att/Trp53tm3Att Gt(ROSA)26Sortm1(cre/ERT2)Tyj/?

        involves: 129S4/SvJae   (conditional)
  • cellular phenotype
  • *normal* cellular phenotype
    • apoptosis observed after tamoxifen treatment and measured 6 hours after gamma irradiation as is observed in controls   (MGI Ref ID J:173395)

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol Trp53tm3Att
Allele Name targeted mutation 3, Laura D Attardi
Allele Type Targeted (Floxed/Frt)
Common Name(s) p53LSL-53,54;
Strain of Origin129S4/SvJae
Gene Symbol and Name Trp53, transformation related protein 53
Chromosome 11
Gene Common Name(s) BCC7; LFS1; P53; p44;
Molecular Note A floxed puromycin/transcription stop cassette was inserted upstream of exon 2. In addition,mutations were inserted in exon 4 altering codon 53 from phenylalanine to glutamine and codon 54 from phenylalanine to serine. The second transactivation domainis incapacitated once the stop sequences are removed by cre expression. [MGI Ref ID J:173395]


Genotyping Information

Genotyping Protocols

Trp53tm3Att, End Point Analysis

Helpful Links

Genotyping resources and troubleshooting


References provided by MGI

Selected Reference(s)

Brady CA; Jiang D; Mello SS; Johnson TM; Jarvis LA; Kozak MM; Kenzelmann Broz D; Basak S; Park EJ; McLaughlin ME; Karnezis AN; Attardi LD. 2011. Distinct p53 transcriptional programs dictate acute DNA-damage responses and tumor suppression. Cell 145(4):571-83. [PubMed: 21565614]  [MGI Ref ID J:173395]

Additional References

Trp53tm3Att related

Jiang D; Brady CA; Johnson TM; Lee EY; Park EJ; Scott MP; Attardi LD. 2011. Full p53 transcriptional activation potential is dispensable for tumor suppression in diverse lineages. Proc Natl Acad Sci U S A 108(41):17123-8. [PubMed: 21969549]  [MGI Ref ID J:177455]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as heterozygotes. Homozygotes exhibit decreased fertility.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


This strain is currently Awaiting Transfer from the Donor.

On 16-APR-13 this strain was accepted for import and is now awaiting transfer to our campus.

Register Interest

View All Strains Awaiting Transfer from the Donor, In Progress and On Hold

Control Information

   Heterozygote from the colony
   Wild-type from the colony
   000664 C57BL/6J (approximate)
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.

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