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| This CAG-CAT-Twist1 transgenic line expresses mouse Twist1, twist homolog 1 (Drosophila), gene in a Cre recombinase inducible fashion, and has applications in studies of craniofacial development, cartilage formation, endochondral ossification and heart valve development. | |||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Former Names FVB-Tg(CAG-cat,Twist1)1Dbsp/J (Changed: 21-JUN-12 ) Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation ?+pN1
Generation DefinitionsDonating Investigator Douglas B Spicer, Maine Medical Center Research Institute Description
The TWIST1 basic helix loop helix transcription factor, plays a role in heart and bone development, as well as lineage specification, differentiation and tumorigenesis. Mutations of TWIST1 in humans are associated with Saethre-Chotzen syndrome and Robinow-Sorauf syndrome. When these CAG-CAT-Twist1 transgenic mice are crossed with a Cre recombinase-expressing strain, the floxed (cat) chloramphenicol acetyltransferase gene is deleted and the CAG promoter drives expression of the mouse Twist1, twist homolog 1 (Drosophila), gene in the cre-expressing cells. The floxed (cat) chloramphenicol acetyltransferase gene serves as a reporter/silencer, preventing expression of Twist1 in the absence of Cre recombinase activity, and as a reporter for transgenic expression. Mice homozygous for the transgenic insert are viable, fertile and exhibit no overt phenotype. The Donating Investigator reports that there are no phenotypic differences between hemizygotes and homozygotes.When bred to a strain with Cre recombinase expression in neural crest cells (see Stock No. 003829 for example), this mutant mouse strain may be useful in studies of craniofacial development.
When bred to a strain with Cre recombinase expression in endothelial cells (see Stock No. 004128 for example), this mutant mouse strain may be useful in studies of heart valve development.
When bred to a strain with Cre recombinase expression in chondrocytes (see Stock No. 003554 for example), this mutant mouse strain may be useful in studies of ossification, growth plate development, and dwarfism.
Development
A transgenic construct containing a chloramphenicol acetyltransferase (cat) gene flanked by loxP sites, upstream of the Twist1, twist homolog 1 (Drosophila), ORF, under the control of the CAG, CMV enhancer/chicken beta-actin promoter, was injected into fertilized FVB mouse eggs. Founder line 57 was subsequently established. The strain was maintained on the FVB background by the Donating Investigator. Upon arrival at The Jackson Laboratory, the mice were crossed to FVB/NJ (Stock No. 001800) at least once to establish the colony.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Twist1
003821 B6.129S1-Twist1Pde/J 002222 B6.129S7-Twist1tm1Bhr/J 002221 B6;129S-Twist1tm1Bhr/J View Strains carrying other alleles of Twist1 (3 strains)
Phenotype
Phenotype Information
- Potential model based on transgenic expression of a human gene that is associated with this disease. Phenotypic similarity to the human disease has not been tested. Craniosynostosis, Type 1; CRS1 (TWIST1)
Robinow-Sorauf Syndrome (TWIST1)
Saethre-Chotzen Syndrome; SCS (TWIST1)
This mouse can be used to support research in many areas including:
Developmental Biology Research
Craniofacial and Palate Defects
Neurobiology Research
Cre-lox System
loxP-flanked Sequences: Test/Reporter
Research Tools
Cre-lox System
loxP-flanked Sequences
loxP-flanked Sequences: Test/Reporter
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(CAG-cat,-Twist1)1Dbsp | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Douglas B Spicer | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | CAG-CAT-Twist; CAGCAT-Twist1; CC-Twist; | ||
| Strain of Origin | FVB | ||
| Expressed Gene | Twist1, twist basic helix-loop-helix transcription factor 1, mouse, laboratory | ||
| Promoter | CMV, cytomegalovirus, human | ||
| Molecular Note | The CAG promoter drives expression of a floxed chloramphenicol acetyltransferase gene and the mouse Twist1 gene. Chloramphenicol acetyltransferase serves as a reporter and a silencer. Twist1 expression occurs only after cre excision of chloramphenicol acetyltransferase. [MGI Ref ID J:108218] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(CAG-cat,Twist1)1Dbsp, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Chakraborty S; Wirrig EE; Hinton RB; Merrill WH; Spicer DB; Yutzey KE. 2010. Twist1 promotes heart valve cell proliferation and extracellular matrix gene expression during development in vivo and is expressed in human diseased aortic valves. Dev Biol 347(1):167-79. [PubMed: 20804746] [MGI Ref ID J:165714]
Connerney J; Andreeva V; Leshem Y; Muentener C; Mercado MA; Spicer DB. 2006. Twist1 dimer selection regulates cranial suture patterning and fusion. Dev Dyn 235(5):1334-46. [PubMed: 16502419] [MGI Ref ID J:108218]
Additional References
Guzzo RM; Andreeva V; Spicer DB; Drissi MH. 2011. Persistent expression of Twist1 in chondrocytes causes growth plate abnormalities and dwarfism in mice. Int J Dev Biol 55(6):641-7. [PubMed: 21769775] [MGI Ref ID J:178268]
Tg(CAG-cat,-Twist1)1Dbsp relatedGuzzo RM; Andreeva V; Spicer DB; Drissi MH. 2011. Persistent expression of Twist1 in chondrocytes causes growth plate abnormalities and dwarfism in mice. Int J Dev Biol 55(6):641-7. [PubMed: 21769775] [MGI Ref ID J:178268]
Vincentz JW; Firulli BA; Lin A; Spicer DB; Howard MJ; Firulli AB. 2013. Twist1 Controls a Cell-Specification Switch Governing Cell Fate Decisions within the Cardiac Neural Crest. PLoS Genet 9(3):e1003405. [PubMed: 23555309] [MGI Ref ID J:195264]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number FGB29
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as hemizygotes or homozygotes. Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Hemizygous for Tg(CAG-cat,-Twist1)1Dbsp
Price per Pair (US dollars $) Pair Genotype $296.00 Hemizygous for Tg(CAG-cat,-Twist1)1Dbsp x Noncarrier $296.00 Noncarrier x Hemizygous for Tg(CAG-cat,-Twist1)1Dbsp Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Hemizygous for Tg(CAG-cat,-Twist1)1Dbsp
Price per Pair (US dollars $) Pair Genotype $384.80 Hemizygous for Tg(CAG-cat,-Twist1)1Dbsp x Noncarrier $384.80 Noncarrier x Hemizygous for Tg(CAG-cat,-Twist1)1Dbsp Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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