Strain Name:

FVB-Tg(CAG-cat,-Twist1)1Dbsp/J

Stock Number:

018543

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Availability:

Cryopreserved - Ready for recovery

This CAG-CAT-Twist1 transgenic line expresses mouse Twist1, twist homolog 1 (Drosophila), gene in a Cre recombinase inducible fashion, and has applications in studies of craniofacial development, cartilage formation, endochondral ossification and heart valve development.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names FVB-Tg(CAG-cat,Twist1)1Dbsp/J    (Changed: 21-JUN-12 )
Type Coisogenic; Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
Generation+pN1
Generation Definitions
 
Donating Investigator Douglas B Spicer,   University of New England

Description
The TWIST1 basic helix loop helix transcription factor, plays a role in heart and bone development, as well as lineage specification, differentiation and tumorigenesis. Mutations of TWIST1 in humans are associated with Saethre-Chotzen syndrome and Robinow-Sorauf syndrome. When these CAG-CAT-Twist1 transgenic mice are crossed with a Cre recombinase-expressing strain, the floxed (cat) chloramphenicol acetyltransferase gene is deleted and the CAG promoter drives expression of the mouse Twist1, twist homolog 1 (Drosophila), gene in the cre-expressing cells. The floxed (cat) chloramphenicol acetyltransferase gene serves as a reporter/silencer, preventing expression of Twist1 in the absence of Cre recombinase activity, and as a reporter for transgenic expression. Mice homozygous for the transgenic insert are viable, fertile and exhibit no overt phenotype. The Donating Investigator reports that there are no phenotypic differences between hemizygotes and homozygotes.

When bred to a strain with Cre recombinase expression in neural crest cells (see Stock No. 003829 for example), this mutant mouse strain may be useful in studies of craniofacial development.

When bred to a strain with Cre recombinase expression in endothelial cells (see Stock No. 004128 for example), this mutant mouse strain may be useful in studies of heart valve development.

When bred to a strain with Cre recombinase expression in chondrocytes (see Stock No. 003554 for example), this mutant mouse strain may be useful in studies of ossification, growth plate development, and dwarfism.

Development
A transgenic construct containing a chloramphenicol acetyltransferase (cat) gene flanked by loxP sites, upstream of the Twist1, twist homolog 1 (Drosophila), ORF, under the control of the CAG, CMV enhancer/chicken beta-actin promoter, was injected into fertilized FVB mouse eggs. Founder line 57 was subsequently established. The strain was maintained on the FVB background by the Donating Investigator. Upon arrival at The Jackson Laboratory, the mice were crossed to FVB/NJ (Stock No. 001800) at least once to establish the colony.

Control Information

  Control
   Noncarrier
   001800 FVB/NJ
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Twist1
003821   B6.129S1-Twist1Pde/J
002222   B6.129S7-Twist1tm1Bhr/J
002221   B6;129S-Twist1tm1Bhr/J
View Strains carrying other alleles of Twist1     (3 strains)

Strains carrying other alleles of CMV
017456   B6(C)-Tg(CAG-mCherry,-GAL4)769Gsn/J
017457   B6(C)-Tg(CAG-mCherry,-GAL4)774Gsn/J
021469   B6(D2)-Tg(CAG-GFP,-Uprt)985Cdoe/J
018439   B6.129S6-Tg(CAG-Bgeo,-SMN2)E9Dscd/J
006054   B6.C-Tg(CMV-cre)1Cgn/J
025855   B6.Cg-Ptprca Lag3tm1Doi Tg(CAG-luc,-GFP)L2G85Chco Thy1a/J
016197   B6.Cg-Tg(CAG-OTC/CAT)4033Prab/J
018021   B6.Cg-Tg(CAG-Tfb1m)AGsha/J
016882   B6.Cg-Tg(CMV-CASP3)14Edge/J
016908   B6.Cg-Tg(CMV-CASP3)17Edge/J
008300   B6.Cg-Tg(CMV-Klc1)73Gsn/J
008301   B6.Cg-Tg(CMV-Klc1)90Gsn/J
007237   B6.Cg-Tg(CMV-Serpine1)1Dgi/J
025854   B6.FVB-Ptprca Tg(CAG-luc,-GFP)L2G85Chco Thy1a/J
018056   B6.FVB-Tg(CAG-boNT/B,-EGFP)U75-56Fwp/J
017524   B6;129-Tg(CMV-Bgeo,-WGA,-ALPP)1Mgmj/J
014605   B6;129S-Tg(CMV-BBS1)6Vcs/J
017954   B6;C-Tg(CAG-Epha4/Efna5,-mCherry)1Slp/J
016532   B6N.FVB(Cg)-Tg(CAG-rtTA3)4288Slowe/J
003465   BALB/c-Tg(CMV-cre)1Cgn/J
010545   C.FVB-Tg(CAG-luc,-GFP)L2G85Chco/FathJ
014564   C57BL/6-Tg(CMV-Tsc2*)1Arbi/KlanJ
006362   C57BL/6J-Tg(CMV-Cox8a/EYFP)17J/J
010548   D1.FVB(Cg)-Tg(CAG-luc,-GFP)L2G85Chco/FathJ
008450   FVB-Tg(CAG-luc,-GFP)L2G85Chco/J
006439   FVB-Tg(tetO/CMV-KRAS*G12C)9.1Msmi/J
018393   FVB/N-Tg(CAG-EGFP,TGFB1*)C8Kul/J
004375   FVB/N-Tg(tetO-Kras2)12Hev/J
004644   NOD.Cg Prkdcscid-Tg(CSF2)2Ygy Tg(IL3)1Ygy Tg(KITLG)3Ygy/YgyJ
013062   NOD.Cg-Prkdcscid Il2rgtm1Wjl Tg(CMV-IL3,CSF2,KITLG)1Eav/MloySzJ
011066   NOD.Cg-Prkdcscid Il2rgtm1Wjl Tg(CSF2)2Ygy Tg(IL3)1Ygy Tg(KITLG)3Ygy/YgyJGckRolyJ
024099   NOD.Cg-Rag1tm1Mom Il2rgtm1Wjl Tg(CMV-IL3,CSF2,KITLG)1Eav/J
010542   NOD.FVB-Tg(CAG-luc,-GFP)L2G85Chco/FathJ
014092   STOCK Tg(ACTB-tTA2,-MAPT/lacZ)1Luo/J
013753   STOCK Tg(CAG-KikGR)33Hadj/J
013754   STOCK Tg(CAG-KikGR)75Hadj/J
019082   STOCK Tg(CMV-GFP,-BBS4)4T25Vcs/J
002471   STOCK Tg(hCMV-cre)140Sau/J
014093   STOCK Tg(tetO-CHRM3*)1Blr/J
View Strains carrying other alleles of CMV     (39 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on transgenic expression of a human gene that is associated with this disease. Phenotypic similarity to the human disease has not been tested.
Craniosynostosis 1; CRS1   (TWIST1)
Robinow-Sorauf Syndrome   (TWIST1)
Saethre-Chotzen Syndrome; SCS   (TWIST1)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.

Tg(CAG-cat,-Twist1)1Dbsp/0 Tg(Col2a1-cre)1Bhr/0

        involves: C57BL/6 * FVB * SJL   (conditional)
  • growth/size/body phenotype
  • decreased body size
    • runted phenotype observed at two weeks of age   (MGI Ref ID J:178268)
    • decreased body weight
      • 36-41% reduction in body mass as compared to control   (MGI Ref ID J:178268)
  • limbs/digits/tail phenotype
  • short limbs
    • first observed at two weeks of age   (MGI Ref ID J:178268)
    • at 8 weeks of age femur and tibia are approximate 14% and 13%, respectively, shorter than controls   (MGI Ref ID J:178268)
  • skeleton phenotype
  • abnormal long bone epiphyseal plate morphology   (MGI Ref ID J:178268)
    • abnormal long bone epiphyseal plate proliferative zone
      • tibial growth plates exhibit disorderly columnar arrays of proliferating chondrocytes and acellular regions in the proliferating zone   (MGI Ref ID J:178268)
      • atypical vascular invasion and focal regions of bony deposition disrupt contiguous columns of chrondocytes   (MGI Ref ID J:178268)
    • increased width of hypertrophic chondrocyte zone
      • width of the tibial growth plate hypertrophic zone is enlarged in four and eight week old mice   (MGI Ref ID J:178268)
  • abnormal ossification involved in bone maturation
    • proportion of cartilage to bone is higher in 2 and 4 week old transgenic mice as compared to controls   (MGI Ref ID J:178268)
  • decreased bone trabecula number
    • reduced trabecular number and increased trabecular spacing   (MGI Ref ID J:178268)
  • decreased trabecular bone thickness   (MGI Ref ID J:178268)
  • decreased trabecular bone volume
    • decrease in trabecular bone volume fraction is observed in transgenic mice at 4 and 8 weeks   (MGI Ref ID J:178268)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Developmental Biology Research
Craniofacial and Palate Defects

Neurobiology Research
Cre-lox System
      loxP-flanked Sequences: Test/Reporter

Research Tools
Cre-lox System
      loxP-flanked Sequences
      loxP-flanked Sequences: Test/Reporter

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(CAG-cat,-Twist1)1Dbsp
Allele Name transgene insertion 1, Douglas B Spicer
Allele Type Transgenic (Inserted expressed sequence)
Common Name(s) CAG-CAT-Twist; CAGCAT-Twist1; CC-Twist;
Strain of OriginFVB
Expressed Gene Twist1, twist basic helix-loop-helix transcription factor 1, mouse, laboratory
Promoter CMV, cytomegalovirus, human
Molecular Note The CAG promoter drives expression of a floxed chloramphenicol acetyltransferase gene and the mouse Twist1 gene. Chloramphenicol acetyltransferase serves as a reporter and a silencer. Twist1 expression occurs only after cre excision of chloramphenicol acetyltransferase. [MGI Ref ID J:108218]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(CAG-cat,Twist1)1Dbsp, High Resolution Melting
Tg(CAG-cat,Twist1)1Dbsp, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Chakraborty S; Wirrig EE; Hinton RB; Merrill WH; Spicer DB; Yutzey KE. 2010. Twist1 promotes heart valve cell proliferation and extracellular matrix gene expression during development in vivo and is expressed in human diseased aortic valves. Dev Biol 347(1):167-79. [PubMed: 20804746]  [MGI Ref ID J:165714]

Connerney J; Andreeva V; Leshem Y; Muentener C; Mercado MA; Spicer DB. 2006. Twist1 dimer selection regulates cranial suture patterning and fusion. Dev Dyn 235(5):1334-46. [PubMed: 16502419]  [MGI Ref ID J:108218]

Additional References

Guzzo RM; Andreeva V; Spicer DB; Drissi MH. 2011. Persistent expression of Twist1 in chondrocytes causes growth plate abnormalities and dwarfism in mice. Int J Dev Biol 55(6):641-7. [PubMed: 21769775]  [MGI Ref ID J:178268]

Tg(CAG-cat,-Twist1)1Dbsp related

Guzzo RM; Andreeva V; Spicer DB; Drissi MH. 2011. Persistent expression of Twist1 in chondrocytes causes growth plate abnormalities and dwarfism in mice. Int J Dev Biol 55(6):641-7. [PubMed: 21769775]  [MGI Ref ID J:178268]

Vincentz JW; Firulli BA; Lin A; Spicer DB; Howard MJ; Firulli AB. 2013. Twist1 Controls a Cell-Specification Switch Governing Cell Fate Decisions within the Cardiac Neural Crest. PLoS Genet 9(3):e1003405. [PubMed: 23555309]  [MGI Ref ID J:195264]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as hemizygotes or homozygotes.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Noncarrier
   001800 FVB/NJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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