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| The Tgfb3 gene is replaced with a Tgfb1 cDNA in these mice, abolishing Tgfb3 expression. TGFB1 expression partially rescues the clefting phenotype associated with Tgfb3 disruption. These mice may be suitable for use in studies examining the isoform-specific roles of transforming growth factors. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Dr. Vesa Kaartinen, University of Michigan Description
In this knock in/out mutant, the transforming growth factor, beta 3 (Tgfb3) coding region was replaced with a transforming growth factor, beta 1 (Tgfb1) cDNA, abolishing Tgfb3 expression. TGFB isoforms are important regulators of cell growth, cell differentiation, apoptosis, and cellular homeostasis. TGFB3 is expressed in the prefusion palatal epithelium and KO models display a cleft of the secondary palate, lung hypoplasia, respiratory distress, hemothorax, and neonatal lethality. When TGFB1 is knocked into the TGFB3 coding region, the clefting phenotype is partially rescued. These mutant mice show partial fusion of the secondary palate. They exhibit a reduction in apoptosis in the midline epithelium during fusion of the palatal shelves, causing anterior and posterior regions of the palate to not fuse. Homozygous mice are viable but do not survive past 2 weeks of age due to failure to feed.Development
A targeting vector was designed to insert transforming growth factor, beta 1 (Tgfb1) cDNA, followed by a polyadenylation sequence and a loxP-flanked neomycin resistance cassette, into the transforming growth factor, beta 3 (Tgfb3) coding region. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric males were bred to B6xCBA F1 females to generate a colony of Tgfb1-KI mice. These mice were maintained on a mixed genetic background. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
Related Strains
Facebase: models
View Facebase: models (58 strains)
002220 B6.129S2-Tgfb1tm1Doe/J 002098 STOCK Tgfb1tm1Doe/J 010721 STOCK Tgfb1tm2.1Doe/J
Phenotype
Phenotype Information
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Camurati-Engelmann Disease; CAEND (TGFB1)
Cystic Fibrosis; CF (TGFB1)
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tgfb3tm2(Tgfb1)Vk/Tgfb3tm2(Tgfb1)Vk
involves: 129S1/Sv * 129X1/SvJ
- mortality/aging
- complete postnatal lethality
- pups do not survive past two weeks of age (MGI Ref ID J:128938)
- craniofacial phenotype
- abnormal nasal septum morphology
- the nasal septum fails to fuse with the anterior palate in E17 embryos (MGI Ref ID J:128938)
- cleft palate
- clefts occur at the junction of the primary and secondary palates and in the posterior region of E17 embryos (MGI Ref ID J:128938)
- behavior/neurological phenotype
- abnormal suckling behavior
- pups fail to suckle (MGI Ref ID J:128938)
- digestive/alimentary phenotype
- cleft palate
- clefts occur at the junction of the primary and secondary palates and in the posterior region of E17 embryos (MGI Ref ID J:128938)
- respiratory system phenotype
- abnormal nasal septum morphology
- the nasal septum fails to fuse with the anterior palate in E17 embryos (MGI Ref ID J:128938)
This mouse can be used to support research in many areas including:
Developmental Biology Research
Craniofacial and Palate Defects
cleft palate and cleft lip
Postnatal Lethality
Homozygous
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tgfb3tm2(Tgfb1)Vk | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Vesa Kaartinen | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | Tgfb1-KI; | ||
| Mutation Made By | Dr. Vesa Kaartinen, University of Michigan | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Expressed Gene | Tgfb1, transforming growth factor, beta 1, mouse, laboratory | ||
| Molecular Note | A vector containing Tgfb1 cDNA followed by a pgk polyA sequence and a loxP flanked neomycin resistance cassette was inserted into the ATG start site of the targeted gene. Quantitative RT-PCR of RNA isolated from the tips of the palatal shelves demonstrates high Tgfb1 expression but no Tgfb3 expression in homozygotes. [MGI Ref ID J:128938] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tgfb3tm2(Tgfb1)Vk,Separated MCA
Tgfb3tm2(Tgfb1)Vk, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Yang LT; Kaartinen V. 2007. Tgfb1 expressed in the Tgfb3 locus partially rescues the cleft palate phenotype of Tgfb3 null mutants. Dev Biol 312(1):384-95. [PubMed: 17967447] [MGI Ref ID J:128938]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, heterozygous mice may be bred together or to wildtype mice from the colony. The donating investigator reports that homozygous mice are viable but do not survive past 2 weeks of age due to failure to feed.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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Contact information
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JAX® Mice, Products & Services Conditions of Use
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