Description

Strain Information

Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Heterozygote x +/+ sibling         (Female x Male)   26-JAN-13 Mating System +/+ sibling x Heterozygote         (Female x Male)   26-JAN-13 Species laboratory mouse Generation ?+pN1 (29-MAR-13) Generation Definitions   Donating Investigator Zipora Yablonka-Reuveni,   University of Washington Donating Investigator Pascal Stuelsatz,   University of Washington

Description
Myf5, myogenic factor 5, is a basic Helix-Loop-Helix transcription factor involved in muscle cell differentiation. These mice carry a targeted mutation in which a beta-galactosidase gene (lacZ) with a nuclear localization signal disrupts the bHLH domain of the protein. Beta galactosidase activity mimics the endogenous expression pattern of the Myf5 gene, and is detected as early as embryonic day 9 in the caudal myotomes and embryonic day 8 in newly differentiated neurons in the mesencephalon. Mice that are heterozygous for the targeted mutation are viable and fertile. Homozygotes die soon after birth due to respiratory failure. Homozygotes also exhibit delayed myotome formation and truncated ribs. No gene product (mRNA) is detected by in situ hybridization analysis of homozygous embryos. Expression of the adjacent Myf6, myogenic factor 6, is not detected by RT-PCR of skeletal muscle from homozygotes, or by whole mount in situ hybridization of homozygous embryos. This strain serves as a reporter for marking cells in the myogenic lineage and for adult muscle stem (satellite) cells.

Development
A targeting vector was used to fuse nlacZ gene (beta-galactosidase gene (lacZ) with a nuclear localization signal) and a tk-NEO cassette in frame into the first exon, 13 amino acids following the ATG codon, disrupting the gene by deleting the bHLH domain (amino acids 14-122). The construct was electroporated into 129S2/SvPas derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into (C57B16 x SJL)F1 2.5 day precompaction morula. The mice were crossed to C57BL/6 and DBA2 mice. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Control Information

	Control
	Wild-type from the colony
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Myf5

007893	B6.129S4-Myf5tm3(cre)Sor/J
010529	B6;129-Myf5tm1(cre)Mrc/J
002522	C.129S4-Myf5tm1Jae/J

View Strains carrying other alleles of Myf5     (3 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Myf5^tm1Pas/Myf5^tm1Pas

        involves: 129S2/SvPas

• muscle phenotype
• abnormal extraocular muscle morphology
  • at birth, mice completely lack extraocular muscles or only have residual myofibers unlike in wild-type mice   (MGI Ref ID J:150130)
• abnormal eye muscle development
  • by E11.5, the extraocular muscle anlage fails to pattern and begins to atrophy unlike in wild-type mice   (MGI Ref ID J:150130)
  • extraocular muscle anlagen exhibits a 5- to 6-fold increase in apoptotic cells compared to in wild-type mice   (MGI Ref ID J:150130)
• embryogenesis phenotype
• delayed somite formation
  • at E11.5, somatic muscle progenitor cells exhibit a temporary pause in development but do not apoptose   (MGI Ref ID J:150130)
  • however, somite fate is rescued thereafter by Myod1 expression   (MGI Ref ID J:150130)
• vision/eye phenotype
• abnormal extraocular muscle morphology
  • at birth, mice completely lack extraocular muscles or only have residual myofibers unlike in wild-type mice   (MGI Ref ID J:150130)
• abnormal eye muscle development
  • by E11.5, the extraocular muscle anlage fails to pattern and begins to atrophy unlike in wild-type mice   (MGI Ref ID J:150130)
  • extraocular muscle anlagen exhibits a 5- to 6-fold increase in apoptotic cells compared to in wild-type mice   (MGI Ref ID J:150130)

Myf5^tm1Pas/Myf5^tm1Pas

        involves: 129/Sv * C57BL/6 * DBA/2

• muscle phenotype
• abnormal muscle progenitor cell migration
  • muscle progenitor cells are abnormally located along the epaxial-most and hypaxial-most dermomyotome lips   (MGI Ref ID J:92799)
• abnormal myotome development
  • at E10 early myotome is missing   (MGI Ref ID J:92799)
• abnormal skeletal muscle morphology
  • dorsal muscles are absent   (MGI Ref ID J:92799)
• cellular phenotype
• abnormal muscle progenitor cell migration
  • muscle progenitor cells are abnormally located along the epaxial-most and hypaxial-most dermomyotome lips   (MGI Ref ID J:92799)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Developmental Biology Research
Mesodermal Defects
      Myogenesis Defects
Perinatal Lethality
      Homozygous

Neurobiology Research
lacZ expression in neural tissue

Research Tools
lacZ Expression
Developmental Biology Research

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Myf5tm1Pas
Allele Name		targeted mutation 1, Institut Pasteur
Allele Type		Targeted (Reporter)
Common Name(s)		Myf5-nlacZ; Myf5lacZ; Myf5nlacZ; myf-5/nlacZ;
Strain of Origin		129S2/SvPas
Site of Expression		Myf5 is a basic Helix-Loop-Helix transcription factor involved in muscle cell differentiation.
Gene Symbol and Name		Myf5, myogenic factor 5
Chromosome		10
Gene Common Name(s)		B130010J22Rik; Myf-5; RIKEN cDNA B130010J22 gene; bHLHc2;
Molecular Note		An nLacZ gene is fused in-frame to the DNA sequences encoding the first 13 amino acids, resulting in a disruption of the gene and expression of nLacZ under the transcriptional regulation of the endogenous promoter. In-situ hybridization analysis indicates that Myf5 and Myf6 expression is absent (J:92799). [MGI Ref ID J:33706] [MGI Ref ID J:92799]

Genotyping

Genotyping Information

Genotyping Protocols

Myf5^tm1Pas,

SEPARATED MELT

Myf5^tm1Pas, Separated PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Tajbakhsh S; Bober E; Babinet C; Pournin S; Arnold H; Buckingham M. 1996. Gene targeting the myf-5 locus with nlacZ reveals expression of this myogenic factor in mature skeletal muscle fibres as well as early embryonic muscle. Dev Dyn 206(3):291-300. [PubMed: 8896984]  [MGI Ref ID J:33706]

Additional References

Day K; Shefer G; Shearer A; Yablonka-Reuveni Z. 2010. The depletion of skeletal muscle satellite cells with age is concomitant with reduced capacity of single progenitors to produce reserve progeny. Dev Biol 340(2):330-43. [PubMed: 20079729]  [MGI Ref ID J:193885]

Kirillova I; Gussoni E; Goldhamer DJ; Yablonka-Reuveni Z. 2007. Myogenic reprogramming of retina-derived cells following their spontaneous fusion with myotubes. Dev Biol 311(2):449-63. [PubMed: 17919536]  [MGI Ref ID J:193808]

Stuelsatz P; Keire P; Almuly R; Yablonka-Reuveni Z. 2012. A contemporary atlas of the mouse diaphragm: myogenicity, vascularity, and the Pax3 connection. J Histochem Cytochem 60(9):638-57. [PubMed: 22723526]  [MGI Ref ID J:193734]

Myf5^tm1Pas related

Anderson C; Williams VC; Moyon B; Daubas P; Tajbakhsh S; Buckingham ME; Shiroishi T; Hughes SM; Borycki AG. 2012. Sonic hedgehog acts cell-autonomously on muscle precursor cells to generate limb muscle diversity. Genes Dev 26(18):2103-17. [PubMed: 22987640]  [MGI Ref ID J:187740]

Autexier C. 2008. POT of gold: modeling dyskeratosis congenita in the mouse. Genes Dev 22(13):1731-6. [PubMed: 18593874]  [MGI Ref ID J:137424]

Bajanca F; Luz M; Raymond K; Martins GG; Sonnenberg A; Tajbakhsh S; Buckingham M; Thorsteinsdottir S. 2006. Integrin {alpha}6{beta}1-laminin interactions regulate early myotome formation in the mouse embryo. Development 133(9):1635-44. [PubMed: 16554364]  [MGI Ref ID J:108443]

Beauchamp JR; Heslop L; Yu DS; Tajbakhsh S; Kelly RG; Wernig A; Buckingham ME; Partridge TA; Zammit PS. 2000. Expression of CD34 and myf5 defines the majority of quiescent adult skeletal muscle satellite cells J Cell Biol 151(6):1221-34. [PubMed: 11121437]  [MGI Ref ID J:66277]

Biressi S; Messina G; Collombat P; Tagliafico E; Monteverde S; Benedetti L; Cusella De Angelis MG; Mansouri A; Ferrari S; Tajbakhsh S; Broccoli V; Cossu G. 2008. The homeobox gene Arx is a novel positive regulator of embryonic myogenesis. Cell Death Differ 15(1):94-104. [PubMed: 17932502]  [MGI Ref ID J:141473]

Borello U; Berarducci B; Murphy P; Bajard L; Buffa V; Piccolo S; Buckingham M; Cossu G. 2006. The Wnt/{beta}-catenin pathway regulates Gli-mediated Myf5 expression during somitogenesis. Development 133(18):3723-32. [PubMed: 16936075]  [MGI Ref ID J:112463]

Borycki AG; Brunk B; Tajbakhsh S; Buckingham M; Chiang C; Emerson CP Jr. 1999. Sonic hedgehog controls epaxial muscle determination through Myf5 activation. Development 126(18):4053-63. [PubMed: 10457014]  [MGI Ref ID J:55780]

Brunelli S; Relaix F; Baesso S; Buckingham M; Cossu G. 2007. Beta catenin-independent activation of MyoD in presomitic mesoderm requires PKC and depends on Pax3 transcriptional activity. Dev Biol 304(2):604-14. [PubMed: 17275805]  [MGI Ref ID J:122535]

Christov C; Chretien F; Abou-Khalil R; Bassez G; Vallet G; Authier FJ; Bassaglia Y; Shinin V; Tajbakhsh S; Chazaud B; Gherardi RK. 2007. Muscle satellite cells and endothelial cells: close neighbors and privileged partners. Mol Biol Cell 18(4):1397-409. [PubMed: 17287398]  [MGI Ref ID J:124055]

Daubas P; Tajbakhsh S; Hadchouel J; Primig M; Buckingham M. 2000. Myf5 is a novel early axonal marker in the mouse brain and is subjected to post-transcriptional regulation in neurons. Development 127(2):319-31. [PubMed: 10603349]  [MGI Ref ID J:56581]

Day K; Shefer G; Shearer A; Yablonka-Reuveni Z. 2010. The depletion of skeletal muscle satellite cells with age is concomitant with reduced capacity of single progenitors to produce reserve progeny. Dev Biol 340(2):330-43. [PubMed: 20079729]  [MGI Ref ID J:193885]

Dellavalle A; Maroli G; Covarello D; Azzoni E; Innocenzi A; Perani L; Antonini S; Sambasivan R; Brunelli S; Tajbakhsh S; Cossu G. 2011. Pericytes resident in postnatal skeletal muscle differentiate into muscle fibres and generate satellite cells. Nat Commun 2:499. [PubMed: 21988915]  [MGI Ref ID J:188175]

Gayraud-Morel B; Chretien F; Flamant P; Gomes D; Zammit PS; Tajbakhsh S. 2007. A role for the myogenic determination gene Myf5 in adult regenerative myogenesis. Dev Biol 312(1):13-28. [PubMed: 17961534]  [MGI Ref ID J:128921]

Georgiadis V; Stewart HJ; Pollard HJ; Tavsanoglu Y; Prasad R; Horwood J; Deltour L; Goldring K; Poirier F; Lawrence-Watt DJ. 2007. Lack of galectin-1 results in defects in myoblast fusion and muscle regeneration. Dev Dyn 236(4):1014-1024. [PubMed: 17366633]  [MGI Ref ID J:119480]

Guardiola O; Lafuste P; Brunelli S; Iaconis S; Touvier T; Mourikis P; De Bock K; Lonardo E; Andolfi G; Bouche A; Liguori GL; Shen MM; Tajbakhsh S; Cossu G; Carmeliet P; Minchiotti G. 2012. Cripto regulates skeletal muscle regeneration and modulates satellite cell determination by antagonizing myostatin. Proc Natl Acad Sci U S A 109(47):E3231-40. [PubMed: 23129614]  [MGI Ref ID J:192264]

Hara M; Yuasa S; Shimoji K; Onizuka T; Hayashiji N; Ohno Y; Arai T; Hattori F; Kaneda R; Kimura K; Makino S; Sano M; Fukuda K. 2011. G-CSF influences mouse skeletal muscle development and regeneration by stimulating myoblast proliferation. J Exp Med 208(4):715-27. [PubMed: 21422169]  [MGI Ref ID J:177323]

Harel I; Maezawa Y; Avraham R; Rinon A; Ma HY; Cross JW; Leviatan N; Hegesh J; Roy A; Jacob-Hirsch J; Rechavi G; Carvajal J; Tole S; Kioussi C; Quaggin S; Tzahor E. 2012. Pharyngeal mesoderm regulatory network controls cardiac and head muscle morphogenesis. Proc Natl Acad Sci U S A 109(46):18839-44. [PubMed: 23112163]  [MGI Ref ID J:191738]

Havis E; Coumailleau P; Bonnet A; Bismuth K; Bonnin MA; Johnson R; Fan CM; Relaix F; Shi DL; Duprez D. 2012. Sim2 prevents entry into the myogenic program by repressing MyoD transcription during limb embryonic myogenesis. Development 139(11):1910-20. [PubMed: 22513369]  [MGI Ref ID J:184003]

Heude E; Bouhali K; Kurihara Y; Kurihara H; Couly G; Janvier P; Levi G. 2010. Jaw muscularization requires Dlx expression by cranial neural crest cells. Proc Natl Acad Sci U S A 107(25):11441-6. [PubMed: 20534536]  [MGI Ref ID J:161382]

Inanlou MR; Kablar B. 2005. Abnormal development of the intercostal muscles and the rib cage in Myf5-/- embryos leads to pulmonary hypoplasia. Dev Dyn 232(1):43-54. [PubMed: 15580568]  [MGI Ref ID J:95138]

Kablar B. 2004. MyoD-lacZ transgenes are early markers in the neural retina, but MyoD function appears to be inhibited in the developing retinal cells. Int J Dev Neurosci 22(4):215-24. [PubMed: 15245757]  [MGI Ref ID J:101960]

Kablar B; Krastel K; Tajbakhsh S; Rudnicki MA. 2003. Myf5 and MyoD activation define independent myogenic compartments during embryonic development. Dev Biol 258(2):307-18. [PubMed: 12798290]  [MGI Ref ID J:83932]

Kablar B; Tajbakhsh S; Rudnicki MA. 2000. Transdifferentiation of esophageal smooth to skeletal muscle is myogenic bHLH factor-dependent. Development 127(8):1627-39. [PubMed: 10725239]  [MGI Ref ID J:60987]

Kafadar KA; Yi L; Ahmad Y; So L; Rossi F; Pavlath GK. 2009. Sca-1 expression is required for efficient remodeling of the extracellular matrix during skeletal muscle regeneration. Dev Biol 326(1):47-59. [PubMed: 19059231]  [MGI Ref ID J:145180]

Kassar-Duchossoy L; Gayraud-Morel B; Gomes D; Rocancourt D; Buckingham M; Shinin V; Tajbakhsh S. 2004. Mrf4 determines skeletal muscle identity in Myf5:Myod double-mutant mice. Nature 431(7007):466-71. [PubMed: 15386014]  [MGI Ref ID J:92799]

Kassar-Duchossoy L; Giacone E; Gayraud-Morel B; Jory A; Gomes D; Tajbakhsh S. 2005. Pax3/Pax7 mark a novel population of primitive myogenic cells during development. Genes Dev 19(12):1426-31. [PubMed: 15964993]  [MGI Ref ID J:98918]

Kirillova I; Gussoni E; Goldhamer DJ; Yablonka-Reuveni Z. 2007. Myogenic reprogramming of retina-derived cells following their spontaneous fusion with myotubes. Dev Biol 311(2):449-63. [PubMed: 17919536]  [MGI Ref ID J:193808]

L'honore A; Ouimette JF; Lavertu-Jolin M; Drouin J. 2010. Pitx2 defines alternate pathways acting through MyoD during limb and somitic myogenesis. Development 137(22):3847-56. [PubMed: 20978076]  [MGI Ref ID J:167064]

Lagha M; Kormish JD; Rocancourt D; Manceau M; Epstein JA; Zaret KS; Relaix F; Buckingham ME. 2008. Pax3 regulation of FGF signaling affects the progression of embryonic progenitor cells into the myogenic program. Genes Dev 22(13):1828-37. [PubMed: 18593883]  [MGI Ref ID J:137423]

Perez-Ruiz A; Gnocchi VF; Zammit PS. 2007. Control of Myf5 activation in adult skeletal myonuclei requires ERK signalling. Cell Signal 19(8):1671-80. [PubMed: 17481856]  [MGI Ref ID J:128629]

Reddy T; Kablar B. 2004. Evidence for the involvement of neurotrophins in muscle transdifferentiation and acetylcholine receptor transformation in the esophagus of Myf5(-/-):MyoD(-/-) and NT-3(-/-) embryos. Dev Dyn 231(4):683-92. [PubMed: 15497153]  [MGI Ref ID J:93814]

Rinon A; Molchadsky A; Nathan E; Yovel G; Rotter V; Sarig R; Tzahor E. 2011. p53 coordinates cranial neural crest cell growth and epithelial-mesenchymal transition/delamination processes. Development 138(9):1827-38. [PubMed: 21447558]  [MGI Ref ID J:171272]

Sambasivan R; Gayraud-Morel B; Dumas G; Cimper C; Paisant S; Kelly R; Tajbakhsh S. 2009. Distinct regulatory cascades govern extraocular and pharyngeal arch muscle progenitor cell fates. Dev Cell 16(6):810-21. [PubMed: 19531352]  [MGI Ref ID J:150130]

Sato T; Rocancourt D; Marques L; Thorsteinsdottir S; Buckingham M. 2010. A Pax3/Dmrt2/Myf5 regulatory cascade functions at the onset of myogenesis. PLoS Genet 6(4):e1000897. [PubMed: 20368965]  [MGI Ref ID J:159213]

Shoji H; Deltour L; Nakamura T; Tajbakhsh S; Poirier F. 2009. Expression pattern and role of Galectin1 during early mouse myogenesis. Dev Growth Differ 51(7):607-15. [PubMed: 19712265]  [MGI Ref ID J:153078]

Stuelsatz P; Keire P; Almuly R; Yablonka-Reuveni Z. 2012. A contemporary atlas of the mouse diaphragm: myogenicity, vascularity, and the Pax3 connection. J Histochem Cytochem 60(9):638-57. [PubMed: 22723526]  [MGI Ref ID J:193734]

Tajbakhsh S; Buckingham ME. 1995. Lineage restriction of the myogenic conversion factor myf-5 in the brain. Development 121(12):4077-83. [PubMed: 8575308]  [MGI Ref ID J:30348]

Tajbakhsh S; Buckingham ME. 1994. Mouse limb muscle is determined in the absence of the earliest myogenic factor myf-5. Proc Natl Acad Sci U S A 91(2):747-51. [PubMed: 8290594]  [MGI Ref ID J:16491]

Tajbakhsh S; Rocancourt D; Buckingham M. 1996. Muscle progenitor cells failing to respond to positional cues adopt non-myogenic fates in myf-5 null mice. Nature 384(6606):266-70. [PubMed: 8918877]  [MGI Ref ID J:36716]

Tajbakhsh S; Rocancourt D; Cossu G; Buckingham M. 1997. Redefining the genetic hierarchies controlling skeletal myogenesis: Pax-3 and Myf-5 act upstream of MyoD. Cell 89(1):127-38. [PubMed: 9094721]  [MGI Ref ID J:39308]

Ugarte G; Cappellari O; Perani L; Pistocchi A; Cossu G. 2012. Noggin recruits mesoderm progenitors from the dorsal aorta to a skeletal myogenic fate. Dev Biol 365(1):91-100. [PubMed: 22370001]  [MGI Ref ID J:184923]

Verma M; Asakura A. 2011. Efficient single muscle fiber isolation from alcohol-fixed adult muscle following beta-galactosidase staining for satellite cell detection. J Histochem Cytochem 59(1):60-7. [PubMed: 20876523]  [MGI Ref ID J:170193]

Verma M; Asakura Y; Hirai H; Watanabe S; Tastad C; Fong GH; Ema M; Call JA; Lowe DA; Asakura A. 2010. Flt-1 haploinsufficiency ameliorates muscular dystrophy phenotype by developmentally increased vasculature in mdx mice. Hum Mol Genet 19(21):4145-59. [PubMed: 20705734]  [MGI Ref ID J:164891]

Vincent SD; Mayeuf A; Niro C; Saitou M; Buckingham M. 2012. Non Conservation of Function for the Evolutionarily Conserved Prdm1 Protein in the Control of the Slow Twitch Myogenic Program in the Mouse Embryo. Mol Biol Evol :. [PubMed: 22522309]  [MGI Ref ID J:186268]

Yoon JK; Olson EN; Arnold HH; Wold BJ. 1997. Different MRF4 knockout alleles differentially disrupt Myf-5 expression: cis-regulatory interactions at the MRF4/Myf-5 locus. Dev Biol 188(2):349-62. [PubMed: 9268580]  [MGI Ref ID J:42453]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX11

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as heterozygotes. Homozygotes die soon after birth due to respiratory failure.
Mating System	Heterozygote x +/+ sibling         (Female x Male)   26-JAN-13
	+/+ sibling x Heterozygote         (Female x Male)   26-JAN-13

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

 

This strain is currently Under Development - Now Accepting Orders.
Estimated Available for Distribution Date: 15-JUL-13

Please note: You may now place orders for this strain although it is not yet ready for distribution. Estimated available for distribution dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for distribution depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain.

Control Information

	Control
	Wild-type from the colony
	000664 C57BL/6J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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