Strain Name:

STOCK Myf5tm1Pas/J

Stock Number:

018626

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Availability:

Cryopreserved - Ready for recovery

This Myf5-nlacZ knock in strain expresses lacZ under the direction of the endogenous Myf5, myogenic factor 5, promoter with beta-galactosidase activity detected in muscle progenitor cell lineages. Applications exist in studies related to myogenesis and neurogenesis.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
Generation+pN1
Generation Definitions
 
Donating Investigator Zipora Yablonka-Reuveni,   University of Washington
Donating Investigator Pascal Stuelsatz,   University of Washington

Description
Myf5, myogenic factor 5, is a basic Helix-Loop-Helix transcription factor involved in muscle cell differentiation. These mice carry a targeted mutation in which a beta-galactosidase gene (lacZ) with a nuclear localization signal disrupts the bHLH domain of the protein. Beta galactosidase activity mimics the endogenous expression pattern of the Myf5 gene, and is detected as early as embryonic day 9 in the caudal myotomes and embryonic day 8 in newly differentiated neurons in the mesencephalon. Mice that are heterozygous for the targeted mutation are viable and fertile. Homozygotes die soon after birth due to respiratory failure. Homozygotes also exhibit delayed myotome formation and truncated ribs. No gene product (mRNA) is detected by in situ hybridization analysis of homozygous embryos. Expression of the adjacent Myf6, myogenic factor 6, is not detected by RT-PCR of skeletal muscle from homozygotes, or by whole mount in situ hybridization of homozygous embryos. This strain serves as a reporter for marking cells in the myogenic lineage and for adult muscle stem (satellite) cells.

Development
A targeting vector was used to fuse nlacZ gene (beta-galactosidase gene (lacZ) with a nuclear localization signal) and a tk-NEO cassette in frame into the first exon, 13 amino acids following the ATG codon, disrupting the gene by deleting the bHLH domain (amino acids 14-122). The construct was electroporated into 129S2/SvPas derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into (C57B16 x SJL)F1 2.5 day precompaction morula. The mice were crossed to C57BL/6 and DBA2 mice. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Myf5
007893   B6.129S4-Myf5tm3(cre)Sor/J
010529   B6;129-Myf5tm1(cre)Mrc/J
002522   C.129S4-Myf5tm1Jae/J
023342   STOCK Myf5tm1(cre/Esr1*)Trdo/J
View Strains carrying other alleles of Myf5     (4 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Myf5tm1Pas/Myf5tm1Pas

        involves: 129S2/SvPas
  • muscle phenotype
  • abnormal extraocular muscle morphology
    • at birth, mice completely lack extraocular muscles or only have residual myofibers unlike in wild-type mice   (MGI Ref ID J:150130)
  • abnormal eye muscle development
    • by E11.5, the extraocular muscle anlage fails to pattern and begins to atrophy unlike in wild-type mice   (MGI Ref ID J:150130)
    • extraocular muscle anlagen exhibits a 5- to 6-fold increase in apoptotic cells compared to in wild-type mice   (MGI Ref ID J:150130)
  • embryogenesis phenotype
  • delayed somite formation
    • at E11.5, somatic muscle progenitor cells exhibit a temporary pause in development but do not apoptose   (MGI Ref ID J:150130)
    • however, somite fate is rescued thereafter by Myod1 expression   (MGI Ref ID J:150130)
  • vision/eye phenotype
  • abnormal extraocular muscle morphology
    • at birth, mice completely lack extraocular muscles or only have residual myofibers unlike in wild-type mice   (MGI Ref ID J:150130)
  • abnormal eye muscle development
    • by E11.5, the extraocular muscle anlage fails to pattern and begins to atrophy unlike in wild-type mice   (MGI Ref ID J:150130)
    • extraocular muscle anlagen exhibits a 5- to 6-fold increase in apoptotic cells compared to in wild-type mice   (MGI Ref ID J:150130)

Myf5tm1Pas/Myf5tm1Pas

        involves: 129/Sv * C57BL/6 * DBA/2
  • muscle phenotype
  • abnormal muscle precursor cell migration
    • muscle progenitor cells are abnormally located along the epaxial-most and hypaxial-most dermomyotome lips   (MGI Ref ID J:92799)
  • abnormal skeletal muscle morphology
    • dorsal muscles are absent   (MGI Ref ID J:92799)
  • absent myotome
    • at E10 early myotome is missing   (MGI Ref ID J:92799)
  • cellular phenotype
  • abnormal muscle precursor cell migration
    • muscle progenitor cells are abnormally located along the epaxial-most and hypaxial-most dermomyotome lips   (MGI Ref ID J:92799)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Developmental Biology Research
Mesodermal Defects
      Myogenesis Defects
Perinatal Lethality
      Homozygous

Neurobiology Research
lacZ expression in neural tissue

Research Tools
lacZ Expression
Developmental Biology Research

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Myf5tm1Pas
Allele Name targeted mutation 1, Institut Pasteur
Allele Type Targeted (Null/Knockout, Reporter)
Common Name(s) Myf5-nlacZ; Myf5lacZ; Myf5nlacZ; myf-5/nlacZ;
Strain of Origin129S2/SvPas
Site of ExpressionMyf5 is a basic Helix-Loop-Helix transcription factor involved in muscle cell differentiation.
Gene Symbol and Name Myf5, myogenic factor 5
Chromosome 10
Gene Common Name(s) B130010J22Rik; Myf-5; RIKEN cDNA B130010J22 gene; bHLHc2;
Molecular Note An nLacZ gene is fused in-frame to the DNA sequences encoding the first 13 amino acids, resulting in a disruption of the gene and expression of nLacZ under the transcriptional regulation of the endogenous promoter. In-situ hybridization analysis indicates that Myf5 and Myf6 expression is absent (J:92799). [MGI Ref ID J:33706] [MGI Ref ID J:92799]

Genotyping

Genotyping Information

Genotyping Protocols

Myf5tm1Pas,

SEPARATED MELT


Myf5tm1Pas, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Tajbakhsh S; Bober E; Babinet C; Pournin S; Arnold H; Buckingham M. 1996. Gene targeting the myf-5 locus with nlacZ reveals expression of this myogenic factor in mature skeletal muscle fibres as well as early embryonic muscle. Dev Dyn 206(3):291-300. [PubMed: 8896984]  [MGI Ref ID J:33706]

Additional References

Day K; Shefer G; Shearer A; Yablonka-Reuveni Z. 2010. The depletion of skeletal muscle satellite cells with age is concomitant with reduced capacity of single progenitors to produce reserve progeny. Dev Biol 340(2):330-43. [PubMed: 20079729]  [MGI Ref ID J:193885]

Kirillova I; Gussoni E; Goldhamer DJ; Yablonka-Reuveni Z. 2007. Myogenic reprogramming of retina-derived cells following their spontaneous fusion with myotubes. Dev Biol 311(2):449-63. [PubMed: 17919536]  [MGI Ref ID J:193808]

Stuelsatz P; Keire P; Almuly R; Yablonka-Reuveni Z. 2012. A contemporary atlas of the mouse diaphragm: myogenicity, vascularity, and the Pax3 connection. J Histochem Cytochem 60(9):638-57. [PubMed: 22723526]  [MGI Ref ID J:193734]

Myf5tm1Pas related

Anderson C; Williams VC; Moyon B; Daubas P; Tajbakhsh S; Buckingham ME; Shiroishi T; Hughes SM; Borycki AG. 2012. Sonic hedgehog acts cell-autonomously on muscle precursor cells to generate limb muscle diversity. Genes Dev 26(18):2103-17. [PubMed: 22987640]  [MGI Ref ID J:187740]

Autexier C. 2008. POT of gold: modeling dyskeratosis congenita in the mouse. Genes Dev 22(13):1731-6. [PubMed: 18593874]  [MGI Ref ID J:137424]

Bajanca F; Luz M; Raymond K; Martins GG; Sonnenberg A; Tajbakhsh S; Buckingham M; Thorsteinsdottir S. 2006. Integrin {alpha}6{beta}1-laminin interactions regulate early myotome formation in the mouse embryo. Development 133(9):1635-44. [PubMed: 16554364]  [MGI Ref ID J:108443]

Beauchamp JR; Heslop L; Yu DS; Tajbakhsh S; Kelly RG; Wernig A; Buckingham ME; Partridge TA; Zammit PS. 2000. Expression of CD34 and myf5 defines the majority of quiescent adult skeletal muscle satellite cells J Cell Biol 151(6):1221-34. [PubMed: 11121437]  [MGI Ref ID J:66277]

Biressi S; Messina G; Collombat P; Tagliafico E; Monteverde S; Benedetti L; Cusella De Angelis MG; Mansouri A; Ferrari S; Tajbakhsh S; Broccoli V; Cossu G. 2008. The homeobox gene Arx is a novel positive regulator of embryonic myogenesis. Cell Death Differ 15(1):94-104. [PubMed: 17932502]  [MGI Ref ID J:141473]

Borello U; Berarducci B; Murphy P; Bajard L; Buffa V; Piccolo S; Buckingham M; Cossu G. 2006. The Wnt/{beta}-catenin pathway regulates Gli-mediated Myf5 expression during somitogenesis. Development 133(18):3723-32. [PubMed: 16936075]  [MGI Ref ID J:112463]

Borycki AG; Brunk B; Tajbakhsh S; Buckingham M; Chiang C; Emerson CP Jr. 1999. Sonic hedgehog controls epaxial muscle determination through Myf5 activation. Development 126(18):4053-63. [PubMed: 10457014]  [MGI Ref ID J:55780]

Brunelli S; Relaix F; Baesso S; Buckingham M; Cossu G. 2007. Beta catenin-independent activation of MyoD in presomitic mesoderm requires PKC and depends on Pax3 transcriptional activity. Dev Biol 304(2):604-14. [PubMed: 17275805]  [MGI Ref ID J:122535]

Christov C; Chretien F; Abou-Khalil R; Bassez G; Vallet G; Authier FJ; Bassaglia Y; Shinin V; Tajbakhsh S; Chazaud B; Gherardi RK. 2007. Muscle satellite cells and endothelial cells: close neighbors and privileged partners. Mol Biol Cell 18(4):1397-409. [PubMed: 17287398]  [MGI Ref ID J:124055]

Daubas P; Tajbakhsh S; Hadchouel J; Primig M; Buckingham M. 2000. Myf5 is a novel early axonal marker in the mouse brain and is subjected to post-transcriptional regulation in neurons. Development 127(2):319-31. [PubMed: 10603349]  [MGI Ref ID J:56581]

Day K; Shefer G; Shearer A; Yablonka-Reuveni Z. 2010. The depletion of skeletal muscle satellite cells with age is concomitant with reduced capacity of single progenitors to produce reserve progeny. Dev Biol 340(2):330-43. [PubMed: 20079729]  [MGI Ref ID J:193885]

Dellavalle A; Maroli G; Covarello D; Azzoni E; Innocenzi A; Perani L; Antonini S; Sambasivan R; Brunelli S; Tajbakhsh S; Cossu G. 2011. Pericytes resident in postnatal skeletal muscle differentiate into muscle fibres and generate satellite cells. Nat Commun 2:499. [PubMed: 21988915]  [MGI Ref ID J:188175]

Gayraud-Morel B; Chretien F; Flamant P; Gomes D; Zammit PS; Tajbakhsh S. 2007. A role for the myogenic determination gene Myf5 in adult regenerative myogenesis. Dev Biol 312(1):13-28. [PubMed: 17961534]  [MGI Ref ID J:128921]

Gayraud-Morel B; Chretien F; Jory A; Sambasivan R; Negroni E; Flamant P; Soubigou G; Coppee JY; Di Santo J; Cumano A; Mouly V; Tajbakhsh S. 2012. Myf5 haploinsufficiency reveals distinct cell fate potentials for adult skeletal muscle stem cells. J Cell Sci 125(Pt 7):1738-49. [PubMed: 22366456]  [MGI Ref ID J:197703]

Georgiadis V; Stewart HJ; Pollard HJ; Tavsanoglu Y; Prasad R; Horwood J; Deltour L; Goldring K; Poirier F; Lawrence-Watt DJ. 2007. Lack of galectin-1 results in defects in myoblast fusion and muscle regeneration. Dev Dyn 236(4):1014-1024. [PubMed: 17366633]  [MGI Ref ID J:119480]

Guardiola O; Lafuste P; Brunelli S; Iaconis S; Touvier T; Mourikis P; De Bock K; Lonardo E; Andolfi G; Bouche A; Liguori GL; Shen MM; Tajbakhsh S; Cossu G; Carmeliet P; Minchiotti G. 2012. Cripto regulates skeletal muscle regeneration and modulates satellite cell determination by antagonizing myostatin. Proc Natl Acad Sci U S A 109(47):E3231-40. [PubMed: 23129614]  [MGI Ref ID J:192264]

Hara M; Yuasa S; Shimoji K; Onizuka T; Hayashiji N; Ohno Y; Arai T; Hattori F; Kaneda R; Kimura K; Makino S; Sano M; Fukuda K. 2011. G-CSF influences mouse skeletal muscle development and regeneration by stimulating myoblast proliferation. J Exp Med 208(4):715-27. [PubMed: 21422169]  [MGI Ref ID J:177323]

Harel I; Maezawa Y; Avraham R; Rinon A; Ma HY; Cross JW; Leviatan N; Hegesh J; Roy A; Jacob-Hirsch J; Rechavi G; Carvajal J; Tole S; Kioussi C; Quaggin S; Tzahor E. 2012. Pharyngeal mesoderm regulatory network controls cardiac and head muscle morphogenesis. Proc Natl Acad Sci U S A 109(46):18839-44. [PubMed: 23112163]  [MGI Ref ID J:191738]

Havis E; Coumailleau P; Bonnet A; Bismuth K; Bonnin MA; Johnson R; Fan CM; Relaix F; Shi DL; Duprez D. 2012. Sim2 prevents entry into the myogenic program by repressing MyoD transcription during limb embryonic myogenesis. Development 139(11):1910-20. [PubMed: 22513369]  [MGI Ref ID J:184003]

Heude E; Bouhali K; Kurihara Y; Kurihara H; Couly G; Janvier P; Levi G. 2010. Jaw muscularization requires Dlx expression by cranial neural crest cells. Proc Natl Acad Sci U S A 107(25):11441-6. [PubMed: 20534536]  [MGI Ref ID J:161382]

Inanlou MR; Kablar B. 2005. Abnormal development of the intercostal muscles and the rib cage in Myf5-/- embryos leads to pulmonary hypoplasia. Dev Dyn 232(1):43-54. [PubMed: 15580568]  [MGI Ref ID J:95138]

Kablar B. 2004. MyoD-lacZ transgenes are early markers in the neural retina, but MyoD function appears to be inhibited in the developing retinal cells. Int J Dev Neurosci 22(4):215-24. [PubMed: 15245757]  [MGI Ref ID J:101960]

Kablar B; Krastel K; Tajbakhsh S; Rudnicki MA. 2003. Myf5 and MyoD activation define independent myogenic compartments during embryonic development. Dev Biol 258(2):307-18. [PubMed: 12798290]  [MGI Ref ID J:83932]

Kablar B; Tajbakhsh S; Rudnicki MA. 2000. Transdifferentiation of esophageal smooth to skeletal muscle is myogenic bHLH factor-dependent. Development 127(8):1627-39. [PubMed: 10725239]  [MGI Ref ID J:60987]

Kafadar KA; Yi L; Ahmad Y; So L; Rossi F; Pavlath GK. 2009. Sca-1 expression is required for efficient remodeling of the extracellular matrix during skeletal muscle regeneration. Dev Biol 326(1):47-59. [PubMed: 19059231]  [MGI Ref ID J:145180]

Kassar-Duchossoy L; Gayraud-Morel B; Gomes D; Rocancourt D; Buckingham M; Shinin V; Tajbakhsh S. 2004. Mrf4 determines skeletal muscle identity in Myf5:Myod double-mutant mice. Nature 431(7007):466-71. [PubMed: 15386014]  [MGI Ref ID J:92799]

Kassar-Duchossoy L; Giacone E; Gayraud-Morel B; Jory A; Gomes D; Tajbakhsh S. 2005. Pax3/Pax7 mark a novel population of primitive myogenic cells during development. Genes Dev 19(12):1426-31. [PubMed: 15964993]  [MGI Ref ID J:98918]

Kirillova I; Gussoni E; Goldhamer DJ; Yablonka-Reuveni Z. 2007. Myogenic reprogramming of retina-derived cells following their spontaneous fusion with myotubes. Dev Biol 311(2):449-63. [PubMed: 17919536]  [MGI Ref ID J:193808]

L'honore A; Ouimette JF; Lavertu-Jolin M; Drouin J. 2010. Pitx2 defines alternate pathways acting through MyoD during limb and somitic myogenesis. Development 137(22):3847-56. [PubMed: 20978076]  [MGI Ref ID J:167064]

Lagha M; Kormish JD; Rocancourt D; Manceau M; Epstein JA; Zaret KS; Relaix F; Buckingham ME. 2008. Pax3 regulation of FGF signaling affects the progression of embryonic progenitor cells into the myogenic program. Genes Dev 22(13):1828-37. [PubMed: 18593883]  [MGI Ref ID J:137423]

Lagha M; Mayeuf-Louchart A; Chang T; Montarras D; Rocancourt D; Zalc A; Kormish J; Zaret KS; Buckingham ME; Relaix F. 2013. Itm2a is a Pax3 target gene, expressed at sites of skeletal muscle formation in vivo. PLoS One 8(5):e63143. [PubMed: 23650549]  [MGI Ref ID J:200546]

Perez-Ruiz A; Gnocchi VF; Zammit PS. 2007. Control of Myf5 activation in adult skeletal myonuclei requires ERK signalling. Cell Signal 19(8):1671-80. [PubMed: 17481856]  [MGI Ref ID J:128629]

Reddy T; Kablar B. 2004. Evidence for the involvement of neurotrophins in muscle transdifferentiation and acetylcholine receptor transformation in the esophagus of Myf5(-/-):MyoD(-/-) and NT-3(-/-) embryos. Dev Dyn 231(4):683-92. [PubMed: 15497153]  [MGI Ref ID J:93814]

Rinon A; Molchadsky A; Nathan E; Yovel G; Rotter V; Sarig R; Tzahor E. 2011. p53 coordinates cranial neural crest cell growth and epithelial-mesenchymal transition/delamination processes. Development 138(9):1827-38. [PubMed: 21447558]  [MGI Ref ID J:171272]

Sambasivan R; Comai G; Le Roux I; Gomes D; Konge J; Dumas G; Cimper C; Tajbakhsh S. 2013. Embryonic founders of adult muscle stem cells are primed by the determination gene Mrf4. Dev Biol 381(1):241-55. [PubMed: 23623977]  [MGI Ref ID J:200727]

Sambasivan R; Gayraud-Morel B; Dumas G; Cimper C; Paisant S; Kelly R; Tajbakhsh S. 2009. Distinct regulatory cascades govern extraocular and pharyngeal arch muscle progenitor cell fates. Dev Cell 16(6):810-21. [PubMed: 19531352]  [MGI Ref ID J:150130]

Sato T; Rocancourt D; Marques L; Thorsteinsdottir S; Buckingham M. 2010. A Pax3/Dmrt2/Myf5 regulatory cascade functions at the onset of myogenesis. PLoS Genet 6(4):e1000897. [PubMed: 20368965]  [MGI Ref ID J:159213]

Shoji H; Deltour L; Nakamura T; Tajbakhsh S; Poirier F. 2009. Expression pattern and role of Galectin1 during early mouse myogenesis. Dev Growth Differ 51(7):607-15. [PubMed: 19712265]  [MGI Ref ID J:153078]

Stuelsatz P; Keire P; Almuly R; Yablonka-Reuveni Z. 2012. A contemporary atlas of the mouse diaphragm: myogenicity, vascularity, and the Pax3 connection. J Histochem Cytochem 60(9):638-57. [PubMed: 22723526]  [MGI Ref ID J:193734]

Tajbakhsh S; Buckingham ME. 1995. Lineage restriction of the myogenic conversion factor myf-5 in the brain. Development 121(12):4077-83. [PubMed: 8575308]  [MGI Ref ID J:30348]

Tajbakhsh S; Buckingham ME. 1994. Mouse limb muscle is determined in the absence of the earliest myogenic factor myf-5. Proc Natl Acad Sci U S A 91(2):747-51. [PubMed: 8290594]  [MGI Ref ID J:16491]

Tajbakhsh S; Rocancourt D; Buckingham M. 1996. Muscle progenitor cells failing to respond to positional cues adopt non-myogenic fates in myf-5 null mice. Nature 384(6606):266-70. [PubMed: 8918877]  [MGI Ref ID J:36716]

Tajbakhsh S; Rocancourt D; Cossu G; Buckingham M. 1997. Redefining the genetic hierarchies controlling skeletal myogenesis: Pax-3 and Myf-5 act upstream of MyoD. Cell 89(1):127-38. [PubMed: 9094721]  [MGI Ref ID J:39308]

Ugarte G; Cappellari O; Perani L; Pistocchi A; Cossu G. 2012. Noggin recruits mesoderm progenitors from the dorsal aorta to a skeletal myogenic fate. Dev Biol 365(1):91-100. [PubMed: 22370001]  [MGI Ref ID J:184923]

Verma M; Asakura A. 2011. Efficient single muscle fiber isolation from alcohol-fixed adult muscle following beta-galactosidase staining for satellite cell detection. J Histochem Cytochem 59(1):60-7. [PubMed: 20876523]  [MGI Ref ID J:170193]

Verma M; Asakura Y; Hirai H; Watanabe S; Tastad C; Fong GH; Ema M; Call JA; Lowe DA; Asakura A. 2010. Flt-1 haploinsufficiency ameliorates muscular dystrophy phenotype by developmentally increased vasculature in mdx mice. Hum Mol Genet 19(21):4145-59. [PubMed: 20705734]  [MGI Ref ID J:164891]

Vincent SD; Mayeuf A; Niro C; Saitou M; Buckingham M. 2012. Non Conservation of Function for the Evolutionarily Conserved Prdm1 Protein in the Control of the Slow Twitch Myogenic Program in the Mouse Embryo. Mol Biol Evol :. [PubMed: 22522309]  [MGI Ref ID J:186268]

Yoon JK; Olson EN; Arnold HH; Wold BJ. 1997. Different MRF4 knockout alleles differentially disrupt Myf-5 expression: cis-regulatory interactions at the MRF4/Myf-5 locus. Dev Biol 188(2):349-62. [PubMed: 9268580]  [MGI Ref ID J:42453]

Yoshida T; Galvez S; Tiwari S; Rezk BM; Semprun-Prieto L; Higashi Y; Sukhanov S; Yablonka-Reuveni Z; Delafontaine P. 2013. Angiotensin II inhibits satellite cell proliferation and prevents skeletal muscle regeneration. J Biol Chem 288(33):23823-32. [PubMed: 23831688]  [MGI Ref ID J:203455]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as heterozygotes. Homozygotes die soon after birth due to respiratory failure.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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Terms of Use


General Terms and Conditions


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JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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