Description

Strain Information

Former Names B6.129S6-Itgb2tm1(CFP)Mskim/J    (Changed: 27-JUN-12 ) Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote         (Female x Male)   09-MAY-13 Species laboratory mouse Generation N7+pN1+F3 (09-MAY-13) Generation Definitions   Donating Investigator Minsoo Kim,   University of Rochester Medical Center

Description
Integrins like Itgb2 (integrin beta 2) are involved with processes of cell adhesion and cell surface-mediated signaling. Mutations in this gene have been associated with leukocyte adhesion deficiency type I (LAD1).

These mice express monomeric cyan fluorescent protein (mCFP) that has been knocked into/fused with the mouse Itgb2 gene. Homozygotes are viable, fertile, show no overt abnormal phenotype, and express normal levels of ITGB2. ITGB2 expression can be visualized directly by live animal imaging without antibody injection or fluorescent cell transfer. All leukocytes expressing ITGB2 express mCFP for purposes of in vivo and ex vivo imaging. These mice are a versatile tool for studying leukocyte behavior as it relates to Itbg2.

Development
mCFP was fused to the last exon (exon 16) of Itgb2 (CD18) gene along with a 6 amino acid linker (GGPVAT) and a loxP-flanked neomycin resistance cassette. The mutation was created in 129S6/SvEvTac-derived W4 embryonic stem (ES) cells. Resultant mice were crossed with a CMV-Cre mouse on a C57BL/6 background to excise the neomycin cassette. This strain was backcrossed to C57BL/6 for seven generations by the donating laboratory.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Itgb2

002128	B6.129S7-Itgb2tm1Bay/J
003329	B6.129S7-Itgb2tm2Bay/J
016897	NOD.129S7(B6)-Itgb2tm2Bay/CgkJ

View Strains carrying other alleles of Itgb2     (3 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI

- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Leukocyte Adhesion Deficiency, Type I; LAD   (ITGB2)

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Itgb2^tm1.1Mskim/Itgb2^tm1.1Mskim

        B6.129S6-Itgb2^tm1.1Mskim

• normal phenotype
• no abnormal phenotype detected
  • mice are fertile and exhibit no obvious phenotypical or functional abnormalities   (MGI Ref ID J:184820)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Research Tools
Fluorescent Proteins
Immunology and Inflammation Research

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Itgb2tm1.1Mskim
Allele Name		targeted mutation 1.1, Minsoo Kim
Allele Type		Targeted (Reporter)
Common Name(s)		CD18-mCFP;
Strain of Origin		129S6/SvEvTac
Site of Expression		Mice express CFP in leukocytes.
Gene Symbol and Name		Itgb2, integrin beta 2
Chromosome		10
Gene Common Name(s)		2E6; AI528527; CD18; CD18 antigen; Cd18; LAD; LCAMB; LFA-1; MAC-1; MF17; MFI7; Mac-1 beta; expressed sequence AI528527;
Molecular Note		mCFP was fused to the last exon (exon 16) along with a 6 amino acid linker (GGPVAT) and a loxP-flanked neomycin resistance cassette. Cre-mediated recombination removed the neo cassette. [MGI Ref ID J:184820]

Genotyping

Genotyping Information

Genotyping Protocols

Generic Neo Melt Curve Analysis, Melt Curve Analysis
Itgb2^{tm1(CFP)Mskim},

Separated MCA

Itgb2^{tm1(CFP)Mskim}, Separated PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Itgb2^tm1.1Mskim related

Hyun YM; Sumagin R; Sarangi PP; Lomakina E; Overstreet MG; Baker CM; Fowell DJ; Waugh RE; Sarelius IH; Kim M. 2012. Uropod elongation is a common final step in leukocyte extravasation through inflamed vessels. J Exp Med :. [PubMed: 22711877]  [MGI Ref ID J:184820]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           FGB29

Colony Maintenance

Breeding & Husbandry	Homozygotes and heterozygotes are viable and fertile.
Mating System	Homozygote x Homozygote         (Female x Male)   09-MAY-13
Diet Information	LabDiet® 5K20

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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