Strain Name:

B6;129S6-Actl6btm1Grc/J

Stock Number:

018783

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Embryonic hippocampal neurons of homozygous Actl6b (actin-like 6B; also known as BAF53b) knockout mice show significantly shorter and less complex dendritic trees than controls. Axonal myelination is impaired in postnatal brains. Surviving adults are hyperactive.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
Generation+pN1
Generation Definitions
 
Donating Investigator Gerald R Crabtree,   Stanford University Medical Center

Description
Actl6b (actin-like 6B; also known as BAF53b) is an actin-related protein that is embryonically expressed in neurons. The protein is part of a brain-specific complex that influences chromatin remodeling, gene expression, and dendritic outgrowth.

These targeted mutation mice carry a knockout allele that eliminates ACTL6B expression. Homozygous mice lacking the protein develop normally in utero, are born at predicted Mendelian frequencies, and appear indistinguishable from their littermates at birth. It is reported, however, that on a mixed 129 and C57BL/6 genetic background, roughly 25% of homozygous pups survive beyond postnatal day 2 and about 12% survive to adulthood. Removal of heterozygous and wildtype sibling pups from litters born to heterozygous or wildtype females is recommended to encourage the survival of homozygous pups. Although fertile, homozygous females fail to care for their young.

Morphologically, the brains of newborn homozygous pups appear grossly normal. Cultured embryonic hippocampal neurons show significantly shorter and less complex dendritic trees than controls. There is also a corresponding reduction in synapse number. Axonal myelination is impaired in postnatal brains. Behaviorally, homozygous adult mice are hyperactive.

Development
Exons 2 and 3 were replaced by a neomycin resistance cassette inserted in reverse transcriptional orientation using TC1 129S6/SvEvTac-derived embryonic stem (ES) cells. This strain was minimally backcrossed to C57BL/6 by the donating laboratory.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Actl6btm1Grc/Actl6btm1Grc

        involves: 129S6/SvEvTac * C57BL/6
  • mortality/aging
  • partial postnatal lethality
    • mice are born at the expected Mendelian ratio, but most die within 2 days   (MGI Ref ID J:132101)
    • about 25% of the homozygous mutant mice survive beyond postnatal day 2 and about 12% survive to adulthood   (MGI Ref ID J:132101)
  • behavior/neurological phenotype
  • hyperactivity
    • mice that escape the perinatal lethality are extremely hyperactive   (MGI Ref ID J:132101)
  • nervous system phenotype
  • abnormal dendrite morphology
    • a significant reduction in activity-dependent dendritic outgrowth and significantly shorter and less complex dendritic trees than controls in long-term low-density hippocampal neuron cultures derived from embryonic brain and in brain slices   (MGI Ref ID J:132101)
  • abnormal myelination
    • reduction in size of the myelinated axon bundles in P18 hippocampal cross section   (MGI Ref ID J:132101)
  • decreased CNS synapse formation
    • synapse formation was severely impaired in long-term low-density hippocampal neuron cultures derived from E18.5 brain   (MGI Ref ID J:132101)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cell Biology Research
Transcriptional Regulation

Developmental Biology Research
Neurodevelopmental Defects
Postnatal Lethality
      Homozygous

Neurobiology Research
Behavioral and Learning Defects
Myelination Defects
Neurodevelopmental Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Actl6btm1Grc
Allele Name targeted mutation 1, Gerald R Crabtree
Allele Type Targeted (Null/Knockout)
Common Name(s) BAF53b-;
Mutation Made By Gerald Crabtree,   Stanford University Medical Center
Strain of Origin129S6/SvEvTac
Gene Symbol and Name Actl6b, actin-like 6B
Chromosome 5
Gene Common Name(s) ACTL6; Actl6; ArpNa; BAF53B; actin-like 6;
Molecular Note Exons 2 and 3 were replaced with a neo cassette. The absence of protein product was confirmed by silver stain analysis on adult brain nuclear extracts. [MGI Ref ID J:132101]

Genotyping

Genotyping Information

Genotyping Protocols

Actl6btm1Grc,

Separated MCA


Actl6btm1Grc, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Wu JI; Lessard J; Olave IA; Qiu Z; Ghosh A; Graef IA; Crabtree GR. 2007. Regulation of dendritic development by neuron-specific chromatin remodeling complexes. Neuron 56(1):94-108. [PubMed: 17920018]  [MGI Ref ID J:132101]

Additional References

Actl6btm1Grc related

Vogel-Ciernia A; Matheos DP; Barrett RM; Kramar EA; Azzawi S; Chen Y; Magnan CN; Zeller M; Sylvain A; Haettig J; Jia Y; Tran A; Dang R; Post RJ; Chabrier M; Babayan AH; Wu JI; Crabtree GR; Baldi P; Baram TZ; Lynch G; Wood MA. 2013. The neuron-specific chromatin regulatory subunit BAF53b is necessary for synaptic plasticity and memory. Nat Neurosci 16(5):552-61. [PubMed: 23525042]  [MGI Ref ID J:197620]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryHeterozygotes are viable and fertile, but homozygous embryos die around the time of implantation.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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