Strain Name:

B6;129S7-Crtaptm1Brle/J

Stock Number:

018831

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Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
Homozygous Crtap (cartilage associated protein) knockout mice develop osteochondrodysplasia characterized by severe osteoporosis and decreased osteoid production. This strain may be useful as a model of osteogenesis imperfecta.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Mating SystemWild-type x Heterozygote         (Female x Male)   25-OCT-12
Mating SystemHeterozygote x Wild-type         (Female x Male)   25-OCT-12
Specieslaboratory mouse
 
Donating Investigator Brendan Lee,   Baylor College of Medicine

Development
A fragment containing the coding sequence of exon 1, intron 1, exon 2, intron 2 and part of exon 3 was replaced with a neomycin cassette using AB2.2 129S7/SvEvBrd-Hprt1+-derived embryonic stem (ES) cells. This strain was maintained on a mixed C57BL/6-129 genetic background by the donating laboratory.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Model with phenotypic similarity to human disease where etiologies involve orthologs. Human genes are associated with this disease. Orthologs of those genes appear in the mouse genotype(s).
Osteogenesis Imperfecta, Type VII; OI7
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Crtaptm1Brle/Crtaptm1Brle

        involves: 129S7/SvEvBrd
  • growth/size/body phenotype
  • fetal growth retardation
    • homozygotes display prenatal growth delay   (MGI Ref ID J:116096)
  • postnatal growth retardation
    • homozygotes display postnatal growth delay   (MGI Ref ID J:116096)
  • skeleton phenotype
  • abnormal bone ossification
    • bone formation rate (BFR) is reduced due to reduction in mineral apposition rate (MAR)   (MGI Ref ID J:116096)
    • abnormal bone mineralization
      • mineralization lag time (Mlt) is reduced; osteoid is mineralizing at faster rate than wild-type   (MGI Ref ID J:116096)
  • abnormal bone structure
    • mutant bones have decreased bone volume/tissue volume ratio   (MGI Ref ID J:116096)
    • abnormal trabecular bone morphology
      • mice show decreased trabecular thickness and trabecular number, while trabecular separation is increased   (MGI Ref ID J:116096)
    • decreased bone mass
      • mice exhibit low bone mass compared to wild-type   (MGI Ref ID J:116096)
      • osteoporosis
        • skeleton shows evidence of marked osteoporosis   (MGI Ref ID J:116096)
  • abnormal osteoblast physiology
    • osteoid is markedly reduced in mutants; cells are producing osteoid at slower rate, as indicated by reduced MAR   (MGI Ref ID J:116096)
  • decreased length of long bones
    • shortening of long bone segments (particularly proximal limb segments - rhizomelia) is manifestation of pre- and postnatal growth delays   (MGI Ref ID J:116096)
  • disorganized long bone epiphyseal plate
    • growth plate shows slightly disorganized columns of proliferating chondrocytes with focal areas of cell drop-out   (MGI Ref ID J:116096)
  • kyphoscoliosis
    • mice develop progressive and severe kyphoscoliosis over the first 6 months of age   (MGI Ref ID J:116096)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Developmental Biology Research
Growth Defects
      Growth Defects (homozygous)
Perinatal Lethality
      Homozygous
Skin and Hair Texture Defects

Internal/Organ Research
Skeleton
      Bone

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Crtaptm1Brle
Allele Name targeted mutation 1, Brendan Lee
Allele Type Targeted (Null/Knockout)
Common Name(s) Crtap-;
Mutation Made By Brendan Lee,   Baylor College of Medicine
Strain of Origin129S7/SvEvBrd-Hprt
Gene Symbol and Name Crtap, cartilage associated protein
Chromosome 9
Gene Common Name(s) 5730529N23Rik; CASP; LEPREL3; OI7; RIKEN cDNA 5730529N23 gene;
Molecular Note A neomycin resistance cassette replaced part of exon 1 and all of exon 2. Absence of transcript was confirmed by Northern blot and RT-PCR analyses. [MGI Ref ID J:116096]

Genotyping

Genotyping Information

Genotyping Protocols

Crtaptm1Brle, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Morello R; Bertin TK; Chen Y; Hicks J; Tonachini L; Monticone M; Castagnola P; Rauch F; Glorieux FH; Vranka J; Bachinger HP; Pace JM; Schwarze U; Byers PH; Weis M; Fernandes RJ; Eyre DR; Yao Z; Boyce BF; Lee B. 2006. CRTAP is required for prolyl 3- hydroxylation and mutations cause recessive osteogenesis imperfecta. Cell 127(2):291-304. [PubMed: 17055431]  [MGI Ref ID J:116096]

Additional References

Crtaptm1Brle related

Baldridge D; Lennington J; Weis M; Homan EP; Jiang MM; Munivez E; Keene DR; Hogue WR; Pyott S; Byers PH; Krakow D; Cohn DH; Eyre DR; Lee B; Morello R. 2010. Generalized connective tissue disease in Crtap-/- mouse. PLoS One 5(5):e10560. [PubMed: 20485499]  [MGI Ref ID J:160834]

Massaad CA; Amin SK; Hu L; Mei Y; Klann E; Pautler RG. 2010. Mitochondrial superoxide contributes to blood flow and axonal transport deficits in the Tg2576 mouse model of Alzheimer's disease. PLoS One 5(5):e10561. [PubMed: 20479943]  [MGI Ref ID J:160835]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryHomozygous knockout mice show some perinatal lethality and surviving homozygous males are not generally good breeders. The donating laboratory maintains the line through heterozygote x heterozygote crosses.
Mating SystemWild-type x Heterozygote         (Female x Male)   25-OCT-12
Heterozygote x Wild-type         (Female x Male)   25-OCT-12

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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