Strain Name:

STOCK Dchs1tm1.1Irv/J

Stock Number:

018851

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Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
These Dchs1 mice possess loxP sites flanking exon 2 of the dachsous 1 (Drosophila) gene. They are useful for generating conditional mutations for studies related to the development of multiple organs/tissues including the kidney, heart, lung and skeleton.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
 
Donating Investigator Kenneth Irvine,   Waksman Institute, Rutgers University

Description
These mice possess loxP sites on either side of exon 2 of the Dchs1 gene. The flanked region involves the first six cadherin domains as well as the initiator methionine and signal peptide.When these mice are bred to mice that express Cre recombinase, the resulting offspring will have the flanked sequence deleted in cre-expressing tissues.

Widespread knockout of Dchs1 (e.g. through crosses with the Tg(Sox2-cre)1Amc strain (Stock No. 004783)) causes defects in multiple internal organs, including kidney, heart, lung and skeleton. Cochleas of homozygous knockouts are shorter than those of wildtype mice. The kidneys are small and cystic. At birth, the lungs are smaller and the intestinal length is reduced. The skeleton exhibits abnormal ossification patterns in the sternum. Although born at predicted Mendelian ratios, homozygous mutants exhibit a variable neonatal lethality, sometimes surviving for two weeks. Homozygous knockout pups are similar in size to their wildtype siblings, but they often have a bent spine and curly tails. They show some signs of postnatal development, such as hair formation, but fail to grow.

Development
Exon 2 was flanked by loxP sites and preceded by a 5' FRT-flanked PGK-neomycin cassette. The mutation was introduced to AB2.2 129S7/SvEvBrd-Hprt1+-derived embryonic stem (ES) cells. Chimeric mice were crossed to C57BL/6J to produce conditional targeting mice. The PGK-neomycin marker was deleted by crossing to 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J (see Stock No. 003946). This strain was maintained on a mixed 129, C57BL/6 and FVB genetic background by the donating laboratory.

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Van Maldergem Syndrome 1; VMLDS1   (DCHS1)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Dchs1tm1.1Irv/Dchs1+

        involves: 129S4/SvJaeSor * 129S7/SvEvBrd * C57BL/6J
  • no phenotypic analysis
  • *normal* no phenotypic analysis   (MGI Ref ID J:169135)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Research Tools
Cardiovascular Research
Cre-lox System
      loxP-flanked Sequences
Developmental Biology Research
      Cre-lox System
Internal/Organ Research

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Dchs1tm1.1Irv
Allele Name targeted mutation 1.1, Kenneth Irvine
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Mutation Made By Kenneth Irvine,   Waksman Institute, Rutgers University
Strain of Origin129S7/SvEvBrd-Hprt
Gene Symbol and Name Dchs1, dachsous 1 (Drosophila)
Chromosome 7
Gene Common Name(s) 3110041P15Rik; C130033F22Rik; CDH19; CDH25; CDHR6; FIB1; Gm165; PCDH16; RIKEN cDNA 3110041P15 gene; RIKEN cDNA C130033F22 gene; VMLDS1; gene model 165, (NCBI);
Molecular Note Exon 2 was flanked by loxP sites and preceded by a 5' FRT-flanked PGK-neomycin cassette. Flp-mediated recombination removed the neo cassette and left exon 2 floxed. [MGI Ref ID J:169135]

Genotyping

Genotyping Information

Genotyping Protocols

Dchs1tm1.1Irv, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Mao Y; Mulvaney J; Zakaria S; Yu T; Morgan KM; Allen S; Basson MA; Francis-West P; Irvine KD. 2011. Characterization of a Dchs1 mutant mouse reveals requirements for Dchs1-Fat4 signaling during mammalian development. Development 138(5):947-57. [PubMed: 21303848]  [MGI Ref ID J:169135]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintained as a live colony, heterozygotes or homozygotes may be bred.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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