Strain Name:

C.129S4(Cg)-Il13tm2.1Lky/J

Stock Number:

018869

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Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
These Smart13 knock-in mice express a modified human CD4 marker from the mouse Il13 gene locus. They are suitable for use in applications where tracking Il13-expressing hematopoietic cells is desired.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names C.Cg-Ptprca Il13tm2.1Lky/J    (Changed: 15-MAR-13 )
CBy.129S4(Cg)-Ptprca Il13tm2(CD4*)Lky/J    (Changed: 24-SEP-12 )
Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Mating SystemHomozygote x Homozygote         (Female x Male)   10-JAN-13
Specieslaboratory mouse
GenerationN10pN1
Generation Definitions
 
Donating Investigator Richard M. Locksley,   Univ of California San Francisco-HHMI

Description
The Smart13 or Il13Smart allele has a bi-cistronic IRES::CD4*F43I cassette inserted between the translational stop codon and the 3' UTR of the interleukin 13 (Il13) gene. These mice express endogenous Il13 and a cell surface membrane-anchored, non-signaling human CD4 marker. Homozygous Smart13 mice are viable and fertile. Expression of the human CD4 marker is observed in Il13-expressing cells including innate type 2 helper cells (Ih2 cells) and type 2 helper T cells (Th2). These Ih2 cells are lineage-negative innate cells that arise during type 2 immunity or in response to the epithelial cytokines IL-25 and IL-33. Ih2 cells are widely distributed in mouse tissues and are particularly prevalent in mesenteric lymph nodes, spleen, and liver.

Development
A targeting vector was designed to insert a neomycin (neo) resistance cassette, and an internal ribosome entry site (IRES), followed by truncated human CD4 cDNA, between the stop codon and the 3' untranslated region of the interleukin 13 (Il13) gene. The truncated CD4 fragment lacking intracellular signaling capacity, also contained a point mutation resulting in an I to F substitution at position 43 of the protein, rendering the human CD4 marker incapable of interacting with mouse major histocompatibility complex class II (MHCII). The construct was electroporated into 129S4/SvJae-derived PrmCre embryonic stem (ES) cells. These PrmCre (or PC3) ES cells contain the Tg(Prm-cre)70Og transgene with the protamine promoter directing cre expression to the male germline. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric males were bred to C57BL/6 females to generate a colony of Il13Smart mice lacking the neo cassette. These mice were backcrossed to BALB/cJ mice (Stock No. 000651) for at least 10 generations. They were then backcrossed with CBy.SJL(B6)-Ptprca/J (Stock No. 006584) to generate Smart13 Ly5.1/5.1 homozygotes. Upon arrival at The Jackson Laboratory, mice were bred to BALB/cJ inbred mice for at least one generation to establish a colony.

Control Information

  Control
   000651 BALB/cJ (approximate)
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying   Ptprca     (7 strains)

Strains carrying other alleles of Il13
015859   C.129P2(Cg)-Il4/Il13tm1.1Lky/J
017353   C.129S4(B6)-Il13tm1(YFP/cre)Lky/J
View Strains carrying other alleles of Il13     (2 strains)

View Strains carrying other alleles of Ptprc     (10 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Allergic Rhinitis   (IL13)
Asthma, Susceptibility to   (IL13)
Hepatitis C Virus, Susceptibility to   (PTPRC)
Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-Negative, B Cell-Positive, Nk Cell-Positive   (PTPRC)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Il13tm2.1Lky/Il13+

        involves: 129S4/SvJae
  • no phenotypic analysis
  • *normal* no phenotypic analysis   (MGI Ref ID J:178986)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Research Tools
Genetics Research
      Tissue/Cell Markers: T cell specific surface marker
Immunology, Inflammation and Autoimmunity Research
      T cell specific surface marker

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Il13tm2.1Lky
Allele Name targeted mutation 2.1, Richard M Locksley
Allele Type Targeted (Reporter)
Common Name(s) IL-13Smart; Il13Smart; Il13tm2.1(CD4)Lky; Smart13;
Strain of Origin129S4/SvJae-Tg(Prm-cre)70Og
Gene Symbol and Name Il13, interleukin 13
Chromosome 11
Gene Common Name(s) IL-13; Il-13; P600;
Molecular Note A targeting vector was designed to insert a neomycin (neo) resistance cassette, and an internal ribosome entry site (IRES), followed by truncated human CD4 cDNA, between the stop codon and the 3' untranslated region. The truncated CD4 fragment lacking intracellular signaling capacity, alsocontained a point mutation resulting in an I to F substitution at position 43 of the protein, rendering the human CD4 marker incapable of interacting with mouse major histocompatibility complex class II (MHCII) and useful as a marker of cells expressing IL13. Cre-mediated recombination removed the neo cassette. [MGI Ref ID J:178986]
 
Allele Symbol Ptprca
Allele Name a variant
Allele Type Not Applicable
Common Name(s) CD45.1; Ly5a; PtprcSJL;
Site of ExpressionWidely expressed on all adaptive and innate immune cells.
Gene Symbol and Name Ptprc, protein tyrosine phosphatase, receptor type, C
Chromosome 1
Gene Common Name(s) B220; CD45; CD45 antigen; CD45R; Cd45; GP180; L-CA; LCA; LY5; Ly-5; Lyt-4; RT7; T-lymphocyte antigen 4; T200; lymphocyte antigen 5;
Molecular Note Ptprca is found in strains SJL/J, STS/A, and DA. Ptprcb is found in strains C57BL/6, C3H/An, DBA/2, AKR, and many others (J:13367, J:12054, J:12077, J:8603). Twelve nucleotide differences between the a and b alleles have been identified. These base substitutions correspond to five amino-acid changes within the extracellular domain of the encoded protein. These amino-acid differences are clustered in a region that also contains the greatest divergence between mouse and rat sequences (J:22485).

Note that the allele designations originally described were reversed in 1987 (J:8603); all publications prior to 1987 show SJL/J, STS/A, and DA as having the b allele and the C57BL/6J group as having the a allele (J:22341). [MGI Ref ID J:12548] [MGI Ref ID J:22485] [MGI Ref ID J:8080]

Genotyping

Genotyping Information

Genotyping Protocols

Il13tm2.1Lky,

SEPARATED MELT


Ptprcab, Pyrosequencing
Il13tm2.1Lky, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Liang HE; Reinhardt RL; Bando JK; Sullivan BM; Ho IC; Locksley RM. 2011. Divergent expression patterns of IL-4 and IL-13 define unique functions in allergic immunity. Nat Immunol 13(1):58-66. [PubMed: 22138715]  [MGI Ref ID J:178986]

Additional References

Il13tm2.1Lky related

Nussbaum JC; Van Dyken SJ; von Moltke J; Cheng LE; Mohapatra A; Molofsky AB; Thornton EE; Krummel MF; Chawla A; Liang HE; Locksley RM. 2013. Type 2 innate lymphoid cells control eosinophil homeostasis. Nature 502(7470):245-8. [PubMed: 24037376]  [MGI Ref ID J:206097]

Ptprca related

Ashbaugh JJ; Brambilla R; Karmally SA; Cabello C; Malek TR; Bethea JR. 2013. IL7Ralpha Contributes to Experimental Autoimmune Encephalomyelitis through Altered T Cell Responses and Nonhematopoietic Cell Lineages. J Immunol 190(9):4525-34. [PubMed: 23530149]  [MGI Ref ID J:195519]

Ataera H; Simkins HM; Hyde E; Yang J; Hermans IF; Petersen TR; Ronchese F. 2013. The control of CD8+ T cell responses is preserved in perforin-deficient mice and released by depletion of CD4+CD25+ regulatory T cells. J Leukoc Biol 94(4):825-33. [PubMed: 23883515]  [MGI Ref ID J:205311]

Ball EA; Sambo MR; Martins M; Trovoada MJ; Benchimol C; Costa J; Antunes Goncalves L; Coutinho A; Penha-Goncalves C. 2013. IFNAR1 controls progression to cerebral malaria in children and CD8+ T cell brain pathology in Plasmodium berghei-infected mice. J Immunol 190(10):5118-27. [PubMed: 23585679]  [MGI Ref ID J:202560]

Basu S; Ray A; Dittel BN. 2011. Cannabinoid receptor 2 is critical for the homing and retention of marginal zone B lineage cells and for efficient T-independent immune responses. J Immunol 187(11):5720-32. [PubMed: 22048769]  [MGI Ref ID J:179699]

Cho RH; Sieburg HB; Muller-Sieburg CE. 2008. A new mechanism for the aging of hematopoietic stem cells: aging changes the clonal composition of the stem cell compartment but not individual stem cells. Blood 111(12):5553-61. [PubMed: 18413859]  [MGI Ref ID J:162992]

Coleman MA; Bridge JA; Lane SW; Dixon CM; Hill GR; Wells JW; Thomas R; Steptoe RJ. 2013. Tolerance induction with gene-modified stem cells and immune-preserving conditioning in primed mice: restricting antigen to differentiated antigen-presenting cells permits efficacy. Blood 121(6):1049-58. [PubMed: 23233664]  [MGI Ref ID J:194614]

De Santo C; Arscott R; Booth S; Karydis I; Jones M; Asher R; Salio M; Middleton M; Cerundolo V. 2010. Invariant NKT cells modulate the suppressive activity of IL-10-secreting neutrophils differentiated with serum amyloid A. Nat Immunol 11(11):1039-46. [PubMed: 20890286]  [MGI Ref ID J:166540]

Del Cid N; Shen L; Belleisle J; Raghavan M. 2012. Assessment of roles for calreticulin in the cross-presentation of soluble and bead-associated antigens. PLoS One 7(7):e41727. [PubMed: 22848581]  [MGI Ref ID J:189698]

Ertelt JM; Buyukbasaran EZ; Jiang TT; Rowe JH; Xin L; Way SS. 2013. B7-1/B7-2 blockade overrides the activation of protective CD8 T cells stimulated in the absence of Foxp3+ regulatory T cells. J Leukoc Biol 94(2):367-76. [PubMed: 23744647]  [MGI Ref ID J:204423]

Ewald SJ; McKenzie IFC. 1979. Immunochemical characterization of the Ly-5 alloantigens on thymocytes. Immunogenetics 8:583-587.  [MGI Ref ID J:13367]

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Gardam S; Turner VM; Anderton H; Limaye S; Basten A; Koentgen F; Vaux DL; Silke J; Brink R. 2011. Deletion of cIAP1 and cIAP2 in murine B lymphocytes constitutively activates cell survival pathways and inactivates the germinal center response. Blood 117(15):4041-51. [PubMed: 21300983]  [MGI Ref ID J:172843]

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Hirahara K; Ghoreschi K; Yang XP; Takahashi H; Laurence A; Vahedi G; Sciume G; Hall AO; Dupont CD; Francisco LM; Chen Q; Tanaka M; Kanno Y; Sun HW; Sharpe AH; Hunter CA; O'Shea JJ. 2012. Interleukin-27 priming of T cells controls IL-17 production in trans via induction of the ligand PD-L1. Immunity 36(6):1017-30. [PubMed: 22726954]  [MGI Ref ID J:187415]

Huber S; Hoffmann R; Muskens F; Voehringer D. 2010. Alternatively activated macrophages inhibit T-cell proliferation by Stat6-dependent expression of PD-L2. Blood 116(17):3311-20. [PubMed: 20625006]  [MGI Ref ID J:165869]

Iijima N; Mattei LM; Iwasaki A. 2011. Recruited inflammatory monocytes stimulate antiviral Th1 immunity in infected tissue. Proc Natl Acad Sci U S A 108(1):284-9. [PubMed: 21173243]  [MGI Ref ID J:169008]

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Jiang S; Alberich-Jorda M; Zagozdzon R; Parmar K; Fu Y; Mauch P; Banu N; Makriyannis A; Tenen DG; Avraham S; Groopman JE; Avraham HK. 2011. Cannabinoid receptor 2 and its agonists mediate hematopoiesis and hematopoietic stem and progenitor cell mobilization. Blood 117(3):827-38. [PubMed: 21063029]  [MGI Ref ID J:168507]

Kasai M; Leclerc JC; Shen FW; Cantor H. 1979. Identification of Ly 5 on the surface of `natural killer' cells in normal and athymic inbred mouse strains. Immunogenetics 8:153-159.  [MGI Ref ID J:12054]

Kitajima M; Ziegler SF. 2013. Cutting edge: identification of the thymic stromal lymphopoietin-responsive dendritic cell subset critical for initiation of type 2 contact hypersensitivity. J Immunol 191(10):4903-7. [PubMed: 24123684]  [MGI Ref ID J:206328]

Kitano M; Moriyama S; Ando Y; Hikida M; Mori Y; Kurosaki T; Okada T. 2011. Bcl6 protein expression shapes pre-germinal center B cell dynamics and follicular helper T cell heterogeneity. Immunity 34(6):961-72. [PubMed: 21636294]  [MGI Ref ID J:174014]

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Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice may be bred together.
Mating SystemHomozygote x Homozygote         (Female x Male)   10-JAN-13

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3175.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $4127.50
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000651 BALB/cJ (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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