Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names C.Cg-Ptprca Il13tm2.1Lky/J    (Changed: 15-MAR-13 ) CBy.129S4(Cg)-Ptprca Il13tm2(CD4*)Lky/J    (Changed: 24-SEP-12 ) Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote         (Female x Male)   10-JAN-13 Species laboratory mouse Generation N10pN1 Generation Definitions   Donating Investigator Richard M. Locksley,   Univ of California San Francisco-HHMI

Description
The Smart13 or Il13^Smart allele has a bi-cistronic IRES::CD4*F43I cassette inserted between the translational stop codon and the 3' UTR of the interleukin 13 (Il13) gene. These mice express endogenous Il13 and a cell surface membrane-anchored, non-signaling human CD4 marker. Homozygous Smart13 mice are viable and fertile. Expression of the human CD4 marker is observed in Il13-expressing cells including innate type 2 helper cells (Ih2 cells) and type 2 helper T cells (Th2). These Ih2 cells are lineage-negative innate cells that arise during type 2 immunity or in response to the epithelial cytokines IL-25 and IL-33. Ih2 cells are widely distributed in mouse tissues and are particularly prevalent in mesenteric lymph nodes, spleen, and liver.

Development
A targeting vector was designed to insert a neomycin (neo) resistance cassette, and an internal ribosome entry site (IRES), followed by truncated human CD4 cDNA, between the stop codon and the 3' untranslated region of the interleukin 13 (Il13) gene. The truncated CD4 fragment lacking intracellular signaling capacity, also contained a point mutation resulting in an I to F substitution at position 43 of the protein, rendering the human CD4 marker incapable of interacting with mouse major histocompatibility complex class II (MHCII). The construct was electroporated into 129S4/SvJae-derived PrmCre embryonic stem (ES) cells. These PrmCre (or PC3) ES cells contain the Tg(Prm-cre)70Og transgene with the protamine promoter directing cre expression to the male germline. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric males were bred to C57BL/6 females to generate a colony of Il13^Smart mice lacking the neo cassette. These mice were backcrossed to BALB/cJ mice (Stock No. 000651) for at least 10 generations. They were then backcrossed with CBy.SJL(B6)-Ptprc^a/J (Stock No. 006584) to generate Smart13 Ly5.1/5.1 homozygotes. Upon arrival at The Jackson Laboratory, mice were bred to BALB/cJ inbred mice for at least one generation to establish a colony.

Control Information

	Control
	000651 BALB/cJ	(approximate)
Considerations for Choosing Controls

Related Strains

Strains carrying   Ptprc^a allele

008451	B6.129P(Cg)-Ptprca Cx3cr1tm1Litt/LittJ
007594	B6.129P2-Ptrpca Ightm1Mnz/J
006259	B6.Cg-Pepcb Ptprca Tg(UBC-scFv)2Nemz/J
013198	B6.Cg-Ptprca Mir223tm1Fcam/J
002014	B6.SJL-Ptprca Pepcb/BoyJ
006584	CBy.SJL(B6)-Ptprca/J

View Strains carrying   Ptprc^a     (6 strains)

Strains carrying other alleles of Il13

015859	C.129P2(Cg)-Il4/Il13tm1.1Lky/J
017353	C.129S4(B6)-Il13tm1(YFP/cre)Lky/J

View Strains carrying other alleles of Il13     (2 strains)

Strains carrying other alleles of Ptprc

007172	129S6.129X1(B6)-Ptprctm1Weis/J
002725	B6.129-Ptprctm1Holm/J
007171	B6.129X1-Ptprctm1Weis/J
017351	BKa.Cg-Ptprcb Bmi1tm1Ilw Thy1a/J
007687	BKa.Cg-Sox17tm1Sjm Ptprcb Thy1a/J
007686	BKa.Cg-Sox17tm2Sjm Ptprcb Thy1a/J
007173	C.129X1(B6)-Ptprctm1Weis/J
016091	C57BL/6-Ptprcltng/J
014149	NOD.B6-Ptprcb/6908MrkTacJ
005616	NOD.C-(Ptprc-D1Mit262)/WehiJ

View Strains carrying other alleles of Ptprc     (10 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI

- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Allergic Rhinitis   (IL13) Asthma, Susceptibility to   (IL13) Hepatitis C Virus, Susceptibility to   (PTPRC) Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-Negative, B Cell-Positive, Nk Cell-Positive   (PTPRC)

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Il13^tm2.1Lky/Il13⁺

        involves: 129S4/SvJae

• no phenotypic analysis
• *normal* no phenotypic analysis   (MGI Ref ID J:178986)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Research Tools
Genetics Research
      Tissue/Cell Markers: T cell specific surface marker
Immunology and Inflammation Research
      T cell specific surface marker

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Il13tm2.1Lky
Allele Name		targeted mutation 2.1, Richard M Locksley
Allele Type		Targeted (Reporter)
Common Name(s)		IL-13Smart; Il13Smart; Il13tm2.1(CD4)Lky; Smart13;
Strain of Origin		129S4/SvJae-Tg(Prm-cre)70Og
Gene Symbol and Name		Il13, interleukin 13
Chromosome		11
Gene Common Name(s)		IL-13; Il-13; P600;
Molecular Note		A targeting vector was designed to insert a neomycin (neo) resistance cassette, and an internal ribosome entry site (IRES), followed by truncated human CD4 cDNA, between the stop codon and the 3' untranslated region. The truncated CD4 fragment lacking intracellular signaling capacity, alsocontained a point mutation resulting in an I to F substitution at position 43 of the protein, rendering the human CD4 marker incapable of interacting with mouse major histocompatibility complex class II (MHCII) and useful as a marker of cells expressing IL13. Cre-mediated recombination removed the neo cassette. [MGI Ref ID J:178986]
Allele Symbol		Ptprca
Allele Name		a variant
Allele Type		Not Applicable
Common Name(s)		CD45.1; Ly5a; PtprcSJL;
Gene Symbol and Name		Ptprc, protein tyrosine phosphatase, receptor type, C
Chromosome		1
Gene Common Name(s)		B220; CD45; CD45 antigen; CD45R; Cd45; GP180; L-CA; LCA; LY5; Ly-5; Lyt-4; RT7; T-lymphocyte antigen 4; T200; lymphocyte antigen 5;
Molecular Note		Ptprca is found in strains SJL/J, STS/A, and DA. Ptprcb is found in strains C57BL/6, C3H/An, DBA/2, AKR, and many others (J:13367, J:12054, J:12077, J:8603). Twelve nucleotide differences between the a and b alleles have been identified. These base substitutions correspond to five amino-acid changes within the extracellular domain of the encoded protein. These amino-acid differences are clustered in a region that also contains the greatest divergence between mouse and rat sequences (J:22485). Note that the allele designations originally described were reversed in 1987 (J:8603); all publications prior to 1987 show SJL/J, STS/A, and DA as having the b allele and the C57BL/6J group as having the a allele (J:22341). [MGI Ref ID J:12548] [MGI Ref ID J:22485] [MGI Ref ID J:8080]

Genotyping

Genotyping Information

Genotyping Protocols

Il13^tm2.1Lky,

SEPARATED MELT

Ptprc^ab, Pyrosequencing
Il13^tm2.1Lky, Separated PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Liang HE; Reinhardt RL; Bando JK; Sullivan BM; Ho IC; Locksley RM. 2011. Divergent expression patterns of IL-4 and IL-13 define unique functions in allergic immunity. Nat Immunol 13(1):58-66. [PubMed: 22138715]  [MGI Ref ID J:178986]

Additional References

Ptprc^a related

Ashbaugh JJ; Brambilla R; Karmally SA; Cabello C; Malek TR; Bethea JR. 2013. IL7Ralpha Contributes to Experimental Autoimmune Encephalomyelitis through Altered T Cell Responses and Nonhematopoietic Cell Lineages. J Immunol 190(9):4525-34. [PubMed: 23530149]  [MGI Ref ID J:195519]

Basu S; Ray A; Dittel BN. 2011. Cannabinoid receptor 2 is critical for the homing and retention of marginal zone B lineage cells and for efficient T-independent immune responses. J Immunol 187(11):5720-32. [PubMed: 22048769]  [MGI Ref ID J:179699]

Cho RH; Sieburg HB; Muller-Sieburg CE. 2008. A new mechanism for the aging of hematopoietic stem cells: aging changes the clonal composition of the stem cell compartment but not individual stem cells. Blood 111(12):5553-61. [PubMed: 18413859]  [MGI Ref ID J:162992]

Coleman MA; Bridge JA; Lane SW; Dixon CM; Hill GR; Wells JW; Thomas R; Steptoe RJ. 2013. Tolerance induction with gene-modified stem cells and immune-preserving conditioning in primed mice: restricting antigen to differentiated antigen-presenting cells permits efficacy. Blood 121(6):1049-58. [PubMed: 23233664]  [MGI Ref ID J:194614]

De Santo C; Arscott R; Booth S; Karydis I; Jones M; Asher R; Salio M; Middleton M; Cerundolo V. 2010. Invariant NKT cells modulate the suppressive activity of IL-10-secreting neutrophils differentiated with serum amyloid A. Nat Immunol 11(11):1039-46. [PubMed: 20890286]  [MGI Ref ID J:166540]

Del Cid N; Shen L; Belleisle J; Raghavan M. 2012. Assessment of roles for calreticulin in the cross-presentation of soluble and bead-associated antigens. PLoS One 7(7):e41727. [PubMed: 22848581]  [MGI Ref ID J:189698]

Ewald SJ; McKenzie IFC. 1979. Immunochemical characterization of the Ly-5 alloantigens on thymocytes. Immunogenetics 8:583-587.  [MGI Ref ID J:13367]

Gardam S; Turner VM; Anderton H; Limaye S; Basten A; Koentgen F; Vaux DL; Silke J; Brink R. 2011. Deletion of cIAP1 and cIAP2 in murine B lymphocytes constitutively activates cell survival pathways and inactivates the germinal center response. Blood 117(15):4041-51. [PubMed: 21300983]  [MGI Ref ID J:172843]

Gerloff J; Korshunov VA. 2012. Immune modulation of vascular resident cells by axl orchestrates carotid intima-media thickening. Am J Pathol 180(5):2134-43. [PubMed: 22538191]  [MGI Ref ID J:183990]

Glass AM; Wolf BJ; Schneider KM; Princiotta MF; Taffet SM. 2013. Connexin43 is dispensable for phagocytosis. J Immunol 190(9):4830-5. [PubMed: 23554311]  [MGI Ref ID J:195494]

Harp JA; Davis BS; Ewald SJ. 1984. Inhibition of T cell responses to alloantigens and polyclonal mitogens by Ly-5 antisera. J Immunol 133(1):10-5. [PubMed: 6233370]  [MGI Ref ID J:7453]

Held W; Clevers H; Grosschedl R. 2003. Redundant functions of TCF-1 and LEF-1 during T and NK cell development, but unique role of TCF-1 for Ly49 NK cell receptor acquisition. Eur J Immunol 33(5):1393-8. [PubMed: 12731066]  [MGI Ref ID J:85394]

Hirahara K; Ghoreschi K; Yang XP; Takahashi H; Laurence A; Vahedi G; Sciume G; Hall AO; Dupont CD; Francisco LM; Chen Q; Tanaka M; Kanno Y; Sun HW; Sharpe AH; Hunter CA; O'Shea JJ. 2012. Interleukin-27 priming of T cells controls IL-17 production in trans via induction of the ligand PD-L1. Immunity 36(6):1017-30. [PubMed: 22726954]  [MGI Ref ID J:187415]

Huber S; Hoffmann R; Muskens F; Voehringer D. 2010. Alternatively activated macrophages inhibit T-cell proliferation by Stat6-dependent expression of PD-L2. Blood 116(17):3311-20. [PubMed: 20625006]  [MGI Ref ID J:165869]

Iijima N; Mattei LM; Iwasaki A. 2011. Recruited inflammatory monocytes stimulate antiviral Th1 immunity in infected tissue. Proc Natl Acad Sci U S A 108(1):284-9. [PubMed: 21173243]  [MGI Ref ID J:169008]

Janowska-Wieczorek A; Majka M; Kijowski J; Baj-Krzyworzeka M; Reca R; Turner AR; Ratajczak J; Emerson SG; Kowalska MA; Ratajczak MZ. 2001. Platelet-derived microparticles bind to hematopoietic stem/progenitor cells and enhance their engraftment. Blood 98(10):3143-9. [PubMed: 11698303]  [MGI Ref ID J:109863]

Jiang S; Alberich-Jorda M; Zagozdzon R; Parmar K; Fu Y; Mauch P; Banu N; Makriyannis A; Tenen DG; Avraham S; Groopman JE; Avraham HK. 2011. Cannabinoid receptor 2 and its agonists mediate hematopoiesis and hematopoietic stem and progenitor cell mobilization. Blood 117(3):827-38. [PubMed: 21063029]  [MGI Ref ID J:168507]

Kasai M; Leclerc JC; Shen FW; Cantor H. 1979. Identification of Ly 5 on the surface of `natural killer' cells in normal and athymic inbred mouse strains. Immunogenetics 8:153-159.  [MGI Ref ID J:12054]

Kitano M; Moriyama S; Ando Y; Hikida M; Mori Y; Kurosaki T; Okada T. 2011. Bcl6 protein expression shapes pre-germinal center B cell dynamics and follicular helper T cell heterogeneity. Immunity 34(6):961-72. [PubMed: 21636294]  [MGI Ref ID J:174014]

Komuro K ; Itakura K ; Boyse EA ; John M. 1975. Ly-5: a new lymphocyte antigen system. Immunogenetics 1:452-456.  [MGI Ref ID J:12077]

Leskov IL; Whitsett J; Vasquez-Vivar J; Stokes KY. 2011. NAD(P)H oxidase and eNOS play differential roles in cytomegalovirus infection-induced microvascular dysfunction. Free Radic Biol Med 51(12):2300-8. [PubMed: 22033010]  [MGI Ref ID J:179407]

Masson F; Minnich M; Olshansky M; Bilic I; Mount AM; Kallies A; Speed TP; Busslinger M; Nutt SL; Belz GT. 2013. Id2-Mediated Inhibition of E2A Represses Memory CD8+ T Cell Differentiation. J Immunol 190(9):4585-94. [PubMed: 23536629]  [MGI Ref ID J:195516]

McAleer JP; Liu B; Li Z; Ngoi SM; Dai J; Oft M; Vella AT. 2010. Potent intestinal Th17 priming through peripheral lipopolysaccharide-based immunization. J Leukoc Biol 88(1):21-31. [PubMed: 20130220]  [MGI Ref ID J:162449]

McNally A; Hill GR; Sparwasser T; Thomas R; Steptoe RJ. 2011. CD4+CD25+ regulatory T cells control CD8+ T-cell effector differentiation by modulating IL-2 homeostasis. Proc Natl Acad Sci U S A 108(18):7529-34. [PubMed: 21502514]  [MGI Ref ID J:172217]

Meisner JK; Song J; Price RJ. 2012. Arteriolar and venular remodeling are differentially regulated by bone marrow-derived cell-specific CX3CR1 and CCR2 expression. PLoS One 7(9):e46312. [PubMed: 23029475]  [MGI Ref ID J:191962]

Mobraaten LE. 1994. Ly5 gene nomenclature, C57BL/6J and SJL/J - A history of change JAX Notes Summer(458):2.  [MGI Ref ID J:22341]

Moebius J; van den Broek M; Groettrup M; Basler M. 2010. Immunoproteasomes are essential for survival and expansion of T cells in virus-infected mice. Eur J Immunol 40(12):3439-49. [PubMed: 21108466]  [MGI Ref ID J:174578]

Morse HC 3rd; Shen FW; Hammerling U. 1987. Genetic nomenclature for loci controlling mouse lymphocyte antigens. Immunogenetics 25(2):71-8. [PubMed: 3817907]  [MGI Ref ID J:8603]

Muller AJ; Filipe-Santos O; Eberl G; Aebischer T; Spath GF; Bousso P. 2012. CD4+ T cells rely on a cytokine gradient to control intracellular pathogens beyond sites of antigen presentation. Immunity 37(1):147-57. [PubMed: 22727490]  [MGI Ref ID J:187413]

Nobrega C; Nunes-Alves C; Cerqueira-Rodrigues B; Roque S; Barreira-Silva P; Behar SM; Correia-Neves M. 2013. T cells home to the thymus and control infection. J Immunol 190(4):1646-58. [PubMed: 23315077]  [MGI Ref ID J:193323]

Pepper M; Pagan AJ; Igyarto BZ; Taylor JJ; Jenkins MK. 2011. Opposing signals from the bcl6 transcription factor and the interleukin-2 receptor generate T helper 1 central and effector memory cells. Immunity 35(4):583-95. [PubMed: 22018468]  [MGI Ref ID J:177635]

Pulliam-Leath AC; Ciccone SL; Nalepa G; Li X; Si Y; Miravalle L; Smith D; Yuan J; Li J; Anur P; Orazi A; Vance GH; Yang FC; Hanenberg H; Bagby GC; Clapp DW. 2010. Genetic disruption of both Fancc and Fancg in mice recapitulates the hematopoietic manifestations of Fanconi anemia. Blood 116(16):2915-20. [PubMed: 20606166]  [MGI Ref ID J:165874]

Raschke WC; Hendricks M; Chen CM. 1995. Genetic basis of antigenic differences between three alleles of Ly5 (CD45) in mice. Immunogenetics 41(2-3):144-7. [PubMed: 7806287]  [MGI Ref ID J:22485]

Robbins CS; Chudnovskiy A; Rauch PJ; Figueiredo JL; Iwamoto Y; Gorbatov R; Etzrodt M; Weber GF; Ueno T; van Rooijen N; Mulligan-Kehoe MJ; Libby P; Nahrendorf M; Pittet MJ; Weissleder R; Swirski FK. 2012. Extramedullary hematopoiesis generates Ly-6C(high) monocytes that infiltrate atherosclerotic lesions. Circulation 125(2):364-74. [PubMed: 22144566]  [MGI Ref ID J:193795]

Roman E; Shino H; Qin FX; Liu YJ. 2010. Cutting edge: Hematopoietic-derived APCs select regulatory T cells in thymus. J Immunol 185(7):3819-23. [PubMed: 20802149]  [MGI Ref ID J:164214]

Schluns KS; Williams K; Ma A; Zheng XX; Lefrancois L. 2002. Cutting edge: requirement for IL-15 in the generation of primary and memory antigen-specific CD8 T cells. J Immunol 168(10):4827-31. [PubMed: 11994430]  [MGI Ref ID J:109854]

Seldin MF; D'Hoostelaere LA; Steinberg AD; Saga Y; Morse HC 3rd. 1987. Allelic variants of Ly-5 in inbred and natural populations of mice. Immunogenetics 26(1-2):74-8. [PubMed: 2886425]  [MGI Ref ID J:8805]

Shang X; Cancelas JA; Li L; Guo F; Liu W; Johnson JF; Ficker A; Daria D; Geiger H; Ratner N; Zheng Y. 2011. R-Ras and Rac GTPase cross-talk regulates hematopoietic progenitor cell migration, homing, and mobilization. J Biol Chem 286(27):24068-78. [PubMed: 21572048]  [MGI Ref ID J:175297]

Shen FW; Saga Y; Litman G; Freeman G; Tung JS; Cantor H; Boyse EA. 1985. Cloning of Ly-5 cDNA. Proc Natl Acad Sci U S A 82(21):7360-3. [PubMed: 3864163]  [MGI Ref ID J:8080]

Shen FW; Tung JS; Boyse EA. 1986. Further definition of the Ly-5 system. Immunogenetics 24(3):146-9. [PubMed: 3489673]  [MGI Ref ID J:8430]

Shulzhenko N; Morgun A; Matzinger P. 2009. Spontaneous mutation in the Cd79b gene leads to a block in B-lymphocyte development at the C' (early pre-B) stage. Genes Immun 10(8):722-6. [PubMed: 19727123]  [MGI Ref ID J:157450]

Siggs OM; Arnold CN; Huber C; Pirie E; Xia Y; Lin P; Nemazee D; Beutler B. 2011. The P4-type ATPase ATP11C is essential for B lymphopoiesis in adult bone marrow. Nat Immunol 12(5):434-40. [PubMed: 21423172]  [MGI Ref ID J:171925]

Silva DG; Daley SR; Hogan J; Lee SK; Teh CE; Hu DY; Lam KP; Goodnow CC; Vinuesa CG. 2011. Anti-islet autoantibodies trigger autoimmune diabetes in the presence of an increased frequency of islet-reactive CD4 T cells. Diabetes 60(8):2102-11. [PubMed: 21788582]  [MGI Ref ID J:186792]

So T; Soroosh P; Eun SY; Altman A; Croft M. 2011. Antigen-independent signalosome of CARMA1, PKC{theta}, and TNF receptor-associated factor 2 (TRAF2) determines NF-{kappa}B signaling in T cells. Proc Natl Acad Sci U S A 108(7):2903-8. [PubMed: 21282629]  [MGI Ref ID J:169068]

Tadagavadi RK; Reeves WB. 2010. Renal dendritic cells ameliorate nephrotoxic acute kidney injury. J Am Soc Nephrol 21(1):53-63. [PubMed: 19875815]  [MGI Ref ID J:185867]

Teng EC; Racioppi L; Means AR. 2011. A cell-intrinsic role for CaMKK2 in granulocyte lineage commitment and differentiation. J Leukoc Biol 90(5):897-909. [PubMed: 21816924]  [MGI Ref ID J:178260]

Waterstrat A; Liang Y; Swiderski CF; Shelton BJ; Van Zant G. 2010. Congenic interval of CD45/Ly-5 congenic mice contains multiple genes that may influence hematopoietic stem cell engraftment. Blood 115(2):408-17. [PubMed: 19901263]  [MGI Ref ID J:155980]

Westmuckett AD; Thacker KM; Moore KL. 2011. Tyrosine sulfation of native mouse psgl-1 is required for optimal leukocyte rolling on p-selectin in vivo. PLoS One 6(5):e20406. [PubMed: 21633705]  [MGI Ref ID J:172569]

Wingender G; Hiss M; Engel I; Peukert K; Ley K; Haller H; Kronenberg M; von Vietinghoff S. 2012. Neutrophilic granulocytes modulate invariant NKT cell function in mice and humans. J Immunol 188(7):3000-8. [PubMed: 22387552]  [MGI Ref ID J:183092]

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de Andres-Aguayo L; Varas F; Kallin EM; Infante JF; Wurst W; Floss T; Graf T. 2011. Musashi 2 is a regulator of the HSC compartment identified by a retroviral insertion screen and knockout mice. Blood 118(3):554-64. [PubMed: 21613258]  [MGI Ref ID J:174870]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, homozygous mice may be bred together.
Mating System	Homozygote x Homozygote         (Female x Male)   10-JAN-13

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Control Information

	Control
	000651 BALB/cJ	(approximate)
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.