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| This strain is useful for transient labeling a rare and transient subpopulation of mouse embryonic stem (ES) cells that lack the typical inner cell mass pluripotency markers found in two-cell (2C) embryos but can contribute to the development of both embryonic and extraembryonic tissues. | |||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Former Names B6(C)-Tg(MERVL-tdTomato)449Slp/J (Changed: 16-AUG-12 ) Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Inbred x Hemizygote (Female x Male) 30-NOV-12 Mating System +/+ sibling x Hemizygote (Female x Male) 30-NOV-12 Species laboratory mouse Generation ?+pN1+F1 (28-MAR-13)
Generation DefinitionsDonating Investigator Samuel Pfaff, The Salk Institute Description
A rare and transient subpopulation of mouse embryonic stem (ES) cells can express high levels of transcripts that are found in two-cell (2C) embryos. These cells lack the typical inner cell mass pluripotency markers Oct4 (Pou5f1), Sox2, and Nanog but can contribute to the development of both embryonic and extraembryonic tissues.The most highly activated sequence repeat in two-cell (2C) embryos is the MuERV-L (murine endogenous retrovirus with leucine tRNA primer) family of retroviruses and their corresponding long terminal repeat (LTR) promoters (Mt2_mm).
In order to label 2C-like ES cells, this transgenic strain places MuERV-L 5' LTR and a portion of the Gag gene upstream of the red fluorescent protein tandem dimeric Tomato (tdTomato).
When paternally inherited, the transgene is expressed in vivo during zygote genome activation at the 2C stage. Fluorescence is weak and can be enhanced using antibodies recognizing the tdTomato transgene.
In vitro, the transgene is expressed in a small population of mouse ES cultures drived from transgene positive blastocysts. tdTomato expression is highest in arrested zygotes and 2C embryos and becomes downregulated at the morula stage.
It was found that these labeled ES cells cycle in and out of the pluripotent 2C-like state. In order to indelibly mark these cells and follow their fate, a transgenic mouse line incorporating MuERV-L regulatory elements driving expression of a tamoxifen-inducible Cre recombinase was created (2C::ERT2-Cre-ERT2) (see Stock No. 018937).
Development
The 5' LTR of murine endogenous retrovirus with leucine tRNA primer (MuERV-L, also called MERVL and Erv4), the primer binding site, and a portion of the Gag gene were placed upstream of red fluorescent protein tandem dimeric Tomato (tdTomato). The transgenic vector was injected into CB6 (BALB/c and C57BL/6) embryos and the resultant strain was subsequently maintained on the same mixed genetic background by the donating laboratory. This is founder line 449.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Erv4
018937 B6;C-Tg(Erv4-cre/ERT2*)657Slp/J View Strains carrying other alleles of Erv4 (1 strain)
Additional Web Information
Use of MICE by companies or for-profit entities requires a license.
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Developmental Biology Research
Research Tools
Developmental Biology Research
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Erv4-tdTomato)449Slp | ||
|---|---|---|---|
| Allele Name | transgene insertion 449, Samuel L Pfaff | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | 2C::tdtomato; MERVL::tdtomato; Tg(MERVL-tdTomato)449Slp; | ||
| Strain of Origin | BALB/c and C57BL/6 | ||
| Site of Expression | The transgene is expressed in a small population of mouse ES cultures drived from transgene positive blastocysts. tdTomato expression is highest in arrested zygotes and 2-cell embryos and becomes downregulated at the morula stage. | ||
| Expressed Gene | RFP, Red Fluorescent Protein, coral | ||
| Red Fluorescent Protein (RFP), derived from marine invertebrate organisms such as the soft coral Discosoma spp and reef coral, Heteractis crispa, is a versatile reporter molecule which has found use in many biological applications. The wild type protein, which is an obligate tetramer, is not well tolerated in mammalian systems. The original molecule has been modified in order to optimize expression to mammalian physiology (examples include monomeric RFP, mRFP1, DsRed, etc). | |||
| Promoter | Erv4, endogenous retroviral sequence 4 (with leucine t-RNA primer), mouse, laboratory | ||
| Molecular Note | The transgene contains the 5' LTR of murine endogenous retrovirus with leucine tRNA primer (MuERV-L, also called MERVL and Erv4), the primer binding site, and a portion of the Gag gene upstream of the red fluorescent protein tandem dimeric Tomato (tdTomato). Line 449 was generated. [MGI Ref ID J:186302] | ||
| Gene Symbol and Name | Tg(Erv4-tdTomato)449Slp, transgene insertion 449, Samuel L Pfaff | ||
| Chromosome | UN | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic tdTomato,SEPARATED MELT
Generic tdTomato, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Macfarlan TS; Gifford WD; Driscoll S; Lettieri K; Rowe HM; Bonanomi D; Firth A; Singer O; Trono D; Pfaff SL. 2012. Embryonic stem cell potency fluctuates with endogenous retrovirus activity. Nature 487(7405):57-63. [PubMed: 22722858] [MGI Ref ID J:186302]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number MGL375
Colony Maintenance
Breeding & Husbandry Hemizygotes are viable, fertile, and have no overt phenotype. It is not yet known whether homozygotes are viable and fertile. When paternally inherited, the transgene is expressed in vivo during zygote genome activation at the embryonic two-cell (2C) stage. Mating System Inbred x Hemizygote (Female x Male) 30-NOV-12 +/+ sibling x Hemizygote (Female x Male) 30-NOV-12
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Hemizygous for Tg(Erv4-tdTomato)449Slp
Price per Pair (US dollars $) Pair Genotype $296.00 Noncarrier x Hemizygous for Tg(Erv4-tdTomato)449Slp Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
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Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Hemizygous for Tg(Erv4-tdTomato)449Slp
Price per Pair (US dollars $) Pair Genotype $384.80 Noncarrier x Hemizygous for Tg(Erv4-tdTomato)449Slp Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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