Strain Name:

B6;C-Tg(Erv4-tdTomato)449Slp/J

Stock Number:

018936

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Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
This strain is useful for transient labeling a rare and transient subpopulation of mouse embryonic stem (ES) cells that lack the typical inner cell mass pluripotency markers found in two-cell (2C) embryos but can contribute to the development of both embryonic and extraembryonic tissues.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6(C)-Tg(MERVL-tdTomato)449Slp/J    (Changed: 16-AUG-12 )
Type Mutant Stock; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
Generation+pN1
Generation Definitions
 
Donating Investigator Samuel Pfaff,   The Salk Institute

Description
A rare and transient subpopulation of mouse embryonic stem (ES) cells can express high levels of transcripts that are found in two-cell (2C) embryos. These cells lack the typical inner cell mass pluripotency markers Oct4 (Pou5f1), Sox2, and Nanog but can contribute to the development of both embryonic and extraembryonic tissues.

The most highly activated sequence repeat in two-cell (2C) embryos is the MuERV-L (murine endogenous retrovirus with leucine tRNA primer) family of retroviruses and their corresponding long terminal repeat (LTR) promoters (Mt2_mm).

In order to label 2C-like ES cells, this transgenic strain places MuERV-L 5' LTR and a portion of the Gag gene upstream of the red fluorescent protein tandem dimeric Tomato (tdTomato).

When paternally inherited, the transgene is expressed in vivo during zygote genome activation at the 2C stage. Fluorescence is weak and can be enhanced using antibodies recognizing the tdTomato transgene.

In vitro, the transgene is expressed in a small population of mouse ES cultures drived from transgene positive blastocysts. tdTomato expression is highest in arrested zygotes and 2C embryos and becomes downregulated at the morula stage.

It was found that these labeled ES cells cycle in and out of the pluripotent 2C-like state. In order to indelibly mark these cells and follow their fate, a transgenic mouse line incorporating MuERV-L regulatory elements driving expression of a tamoxifen-inducible Cre recombinase was created (2C::ERT2-Cre-ERT2) (see Stock No. 018937).

Development
The 5' LTR of murine endogenous retrovirus with leucine tRNA primer (MuERV-L, also called MERVL and Erv4), the primer binding site, and a portion of the Gag gene were placed upstream of red fluorescent protein tandem dimeric Tomato (tdTomato). The transgenic vector was injected into CB6 (BALB/c and C57BL/6) embryos and the resultant strain was subsequently maintained on the same mixed genetic background by the donating laboratory. This is founder line 449.

Control Information

  Control
   Noncarrier
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Erv4
018937   B6;C-Tg(Erv4-cre/ERT2*)657Slp/J
View Strains carrying other alleles of Erv4     (1 strain)

Strains carrying other alleles of RFP
006067   129-Gt(ROSA)26Sortm2(CAG-Dsred2/EGFP)Luo/J
006041   129-Gt(ROSA)26Sortm3(CAG-EGFP/Dsred2)Luo/J
012687   B6(129S4)-Tg(SYN1-icre/mRFP1)9934Rdav/J
017456   B6(C)-Tg(CAG-mCherry,-GAL4)769Gsn/J
017457   B6(C)-Tg(CAG-mCherry,-GAL4)774Gsn/J
017614   B6(Cg)-Tyrc-2J Tg(UBC-mCherry)1Phbs/J
021011   B6(D2)-Tg(CAG-Brainbow1.0)2Eggn/J
021012   B6(D2)-Tg(CAG-Brainbow1.0)3Eggn/J
007676   B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J
006080   B6.129-Gt(ROSA)26Sortm2(CAG-Dsred2/EGFP)Luo/J
006075   B6.129-Gt(ROSA)26Sortm3(CAG-EGFP/Dsred2)Luo/J
007914   B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J
007909   B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J
017863   B6.Cg-Tg(Adora2a-Chrm3*,-mCherry)AD6Blr/J
005884   B6.Cg-Tg(CAG-mRFP1)1F1Hadj/J
016204   B6.Cg-Tg(Drd1a-tdTomato)6Calak/J
018410   B6.Cg-Tg(Pnkd*A7V*A9V,-DsRed)671Ljp/J
022146   B6.Cg-Tg(Pnkd*A7V*A9V,-DsRed)704Ljp/J
022147   B6.Cg-Tg(Pnkd,-DsRed)445Ljp/J
007901   B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J
007911   B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J
007921   B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J
010984   B6;129P-Upk1btm1Pzg/J
013139   B6;129P2-Ifitm3tm1(RFP)Pzg/J
006720   B6;129P2-Olfr124tm4Mom/MomJ
010983   B6;129S-Id3tm1Pzg/J
010986   B6;129S-Osr2tm1Pzg/J
018128   B6;129S-Tg(Prox1-tdTomato)12Nrud/J
007908   B6;129S6-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J
007905   B6;129S6-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J
017954   B6;C-Tg(CAG-Epha4/Efna5,-mCherry)1Slp/J
007984   B6;CBA-Tg(H/Olfr16-taumCherry,-tauGFP)11Mom/MomJ
007985   B6;CBA-Tg(H/Olfr16-taumCherry,-tauGFP)13Mom/MomJ
007982   B6;CBA-Tg(H/Olfr16-taumRFP,-tauGFP)8Mom/MomJ
007983   B6;CBA-Tg(H/Olfr16-taumRFP,-tauGFP)9Mom/MomJ
007910   B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J
017496   B6;D-Tg(Lfng-TagRFP/cre/ERT2)8Amc/J
018682   B6;D-Tg(Meox1-TagRFP/cre/ERT2)30Amc/J
018683   B6;D-Tg(Wnt11-TagRFP/cre/ERT2)28Amc/J
013137   B6;D2-Tg(Akr1b7-RFP)9Amc/J
021577   B6;D2-Tg(Myh6*-mCherry)2Mik/J
015853   B6;DBA-Tg(Cited1-TagRFP)26Amc/J
008374   C57BL/6-Foxp3tm1Flv/J
024046   C57BL/6-Tg(Csf1r-HBEGF/mCherry)1Mnz/J
022766   C57BL/6-Tg(Prox1-tdTomato)12Nrud/J
021119   C57BL/6J-Tg(Dlx2-cre,-mCherry)4Grsr/GrsrJ
021423   C57BL/6J-Tg(Dlx2-cre,-mCherry)9Grsr/GrsrJ
018754   C57BL/6J-Tg(Tbx22,-cre,-mCherry)1Grsr/GrsrJ
018542   FVB-Tg(Kdr-mCherry)1Medi/J
021853   FVB-Tg(Myh6-mCherry/Map1lc3a)1Rag/J
018067   FVB-Tg(Prism)1849Htz/J
018071   FVB-Tg(Prism)1861Htz/J
018068   FVB-Tg(Prism)1989Htz/J
007576   STOCK Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J
013749   STOCK Iis2tm1(ACTB-EGFP,-tdTomato)Luo/J
017530   STOCK Iis2tm2(ACTB-tdTomato,-EGFP)Luo Trp53tm1Tyj Nf1tm1Par/J
013751   STOCK Iis2tm2(ACTB-tdTomato,-EGFP)Luo/J
017923   STOCK Iis3tm2.1(ACTB-EGFP*,-tdTomato)Luo/J
017472   STOCK Tg(Acp5-CFP,Ibsp-YFP,Dmp1-RFP)1Pmay/J
014177   STOCK Tg(Afp-mCherry)1Hadj/J
013753   STOCK Tg(CAG-KikGR)33Hadj/J
013754   STOCK Tg(CAG-KikGR)75Hadj/J
005645   STOCK Tg(CAG-mRFP1)1F1Hadj/J
017465   STOCK Tg(Col10a1-mCherry)3Pmay/J
016921   STOCK Tg(Myh2-DsRed2)1Jrs/J
021226   STOCK Tg(Thy1-Brainbow3.1)18Jrs/J
021225   STOCK Tg(Thy1-Brainbow3.1)3Jrs/J
021227   STOCK Tg(Thy1-Brainbow3.2)7Jrs/J
017470   STOCK Tg(Tnc-mCherry)2Pmay/J
011108   STOCK Tg(Ttr-RFP)1Hadj/J
016981   STOCK Tg(Uchl1-HIST2H2BE/mCherry/EGFP*)FSout/J
017906   STOCK Tg(tetO-hop/EGFP,-COP4/mCherry)6Kftnk/J
View Strains carrying other alleles of RFP     (72 strains)

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Developmental Biology Research

Research Tools
Developmental Biology Research
Fluorescent Proteins

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(Erv4-tdTomato)449Slp
Allele Name transgene insertion 449, Samuel L Pfaff
Allele Type Transgenic (Reporter)
Common Name(s) 2C::tdtomato; MERVL::tdtomato; Tg(MERVL-tdTomato)449Slp;
Strain of OriginBALB/c and C57BL/6
Site of ExpressionThe transgene is expressed in a small population of mouse ES cultures drived from transgene positive blastocysts. tdTomato expression is highest in arrested zygotes and 2-cell embryos and becomes downregulated at the morula stage.
Expressed Gene RFP, Red Fluorescent Protein, coral
Red Fluorescent Protein (RFP), derived from marine invertebrate organisms such as the soft coral Discosoma spp and reef coral, Heteractis crispa, is a versatile reporter molecule which has found use in many biological applications. The wild type protein, which is an obligate tetramer, is not well tolerated in mammalian systems. The original molecule has been modified in order to optimize expression to mammalian physiology (examples include monomeric RFP, mRFP1, DsRed, etc).
Promoter Erv4, endogenous retroviral sequence 4 (with leucine t-RNA primer), mouse, laboratory
Molecular Note The transgene contains the 5' LTR of murine endogenous retrovirus with leucine tRNA primer (MuERV-L, also called MERVL and Erv4), the primer binding site, and a portion of the Gag gene upstream of the red fluorescent protein tandem dimeric Tomato (tdTomato). Line 449 was generated. [MGI Ref ID J:186302]
 
 
Gene Symbol and Name Tg(Erv4-tdTomato)449Slp, transgene insertion 449, Samuel L Pfaff
Chromosome UN

Genotyping

Genotyping Information

Genotyping Protocols

Generic tdTomato,

MELT


Generic tdTomato, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Macfarlan TS; Gifford WD; Driscoll S; Lettieri K; Rowe HM; Bonanomi D; Firth A; Singer O; Trono D; Pfaff SL. 2012. Embryonic stem cell potency fluctuates with endogenous retrovirus activity. Nature 487(7405):57-63. [PubMed: 22722858]  [MGI Ref ID J:186302]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryHemizygotes are viable, fertile, and have no overt phenotype. It is not yet known whether homozygotes are viable and fertile. When paternally inherited, the transgene is expressed in vivo during zygote genome activation at the embryonic two-cell (2C) stage.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2085.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2710.50
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Noncarrier
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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