Strain Name:

B6.Cg-Prkchtm1.2Gasc/J

Stock Number:

018988

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Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
PKCeta-/- mice may be useful for studying T cell homeostasis and activation.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Generation+pN1
Generation Definitions
 
Donating Investigator Nicholas Gascoigne,   The Scripps Research Institute

Description
Exon 2 of the protein kinase C, eta (Prkch) gene was removed, abolishing gene function. These PKCeta-/- homozygotes are viable and fertile. Prkch-encoded PKCeta is a serine/threonine kinase abundant in T cells which is recruited during positive selection of thymocytes. T-cells from these PKCeta-/- mice showed poor proliferation in response to antigen stimulation and exhibited defective homeostatic proliferation. These mice also have a higher ratio of CD4+ to CD8+ T cells compared to that of wild-type mice.

Development
A targeting vector was designed to insert a loxP site, followed by a frt-flanked neomycin resistance (neo) cassette, upstream of exon 2 and a second loxP site downstream of exon 2 of the protein kinase C, eta (Prkch) gene. The construct was electroporated into C57BL/6-derived Brunce-4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric males were bred to C57BL/6J females. Offspring were bred with B6.Cg-Tg(ACTFLPe)9205Dym/J (Stock No. 005703) to delete the neo cassette, then the progeny were bred with B6.FVB-Tg(EIIa-cre)C5379Lmgd/J (Stock No. 003724) transgenic mice to delete exon 2. progeny were crossed to remove the cre- and Flp-expressing transgenes. These mice were then backcrossed to C57BL/6J mice for at least 4 generations. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Control Information

  Control
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Stroke, Ischemic   (PRKCH)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Prkchtm1.2Gasc/Prkchtm1.2Gasc

        involves: C57BL/6 * FVB/N * SJL
  • immune system phenotype
  • abnormal effector T cell morphology
    • the ratio of CD4 to CD8 T cells is higher than in wild-type mice   (MGI Ref ID J:186080)
    • increased CD4-positive, alpha beta T cell number
      • in the lymph node   (MGI Ref ID J:186080)
    • increased CD8-positive, alpha-beta T cell number
      • in the lymph node   (MGI Ref ID J:186080)
  • abnormal lymph node morphology
    • increased lymph node cell number   (MGI Ref ID J:186080)
    • abnormal brachial lymph node morphology
    • abnormal cervical lymph node morphology
      • enlarged and increased in number   (MGI Ref ID J:186080)
      • enlarged cervical lymph nodes   (MGI Ref ID J:186080)
    • enlarged axillary lymph nodes   (MGI Ref ID J:186080)
    • enlarged inguinal lymph nodes   (MGI Ref ID J:186080)
    • enlarged lymph nodes
      • cervical, axillar, brachial and inguinal lymph nodes   (MGI Ref ID J:186080)
  • decreased T cell proliferation
    • when stimulated by anti-CD3 antibodies or antigens (in vitro and in vivo)   (MGI Ref ID J:186080)
  • decreased lymphocyte cell number
    • in the spleen   (MGI Ref ID J:186080)
    • decreased B cell number
      • in the spleen   (MGI Ref ID J:186080)
    • decreased T cell number
      • in the spleen   (MGI Ref ID J:186080)
      • decreased CD4-positive, alpha beta T cell number
        • in the spleen   (MGI Ref ID J:186080)
      • decreased CD8-positive, alpha-beta T cell number
        • in the spleen   (MGI Ref ID J:186080)
      • decreased DN1 thymic pro-T cell number   (MGI Ref ID J:186080)
      • decreased DN4 thymocyte number   (MGI Ref ID J:186080)
      • decreased double-positive T cell number
  • increased B cell number
    • in the lymph node   (MGI Ref ID J:186080)
  • increased T cell number
    • in the lymph node   (MGI Ref ID J:186080)
    • increased CD4-positive, alpha beta T cell number
      • in the lymph node   (MGI Ref ID J:186080)
    • increased CD8-positive, alpha-beta T cell number
      • in the lymph node   (MGI Ref ID J:186080)
    • increased memory T cell number   (MGI Ref ID J:186080)
  • thymus hypoplasia   (MGI Ref ID J:186080)
  • hematopoietic system phenotype
  • abnormal effector T cell morphology
    • the ratio of CD4 to CD8 T cells is higher than in wild-type mice   (MGI Ref ID J:186080)
    • increased CD4-positive, alpha beta T cell number
      • in the lymph node   (MGI Ref ID J:186080)
    • increased CD8-positive, alpha-beta T cell number
      • in the lymph node   (MGI Ref ID J:186080)
  • decreased T cell proliferation
    • when stimulated by anti-CD3 antibodies or antigens (in vitro and in vivo)   (MGI Ref ID J:186080)
  • decreased lymphocyte cell number
    • in the spleen   (MGI Ref ID J:186080)
    • decreased B cell number
      • in the spleen   (MGI Ref ID J:186080)
    • decreased T cell number
      • in the spleen   (MGI Ref ID J:186080)
      • decreased CD4-positive, alpha beta T cell number
        • in the spleen   (MGI Ref ID J:186080)
      • decreased CD8-positive, alpha-beta T cell number
        • in the spleen   (MGI Ref ID J:186080)
      • decreased DN1 thymic pro-T cell number   (MGI Ref ID J:186080)
      • decreased DN4 thymocyte number   (MGI Ref ID J:186080)
      • decreased double-positive T cell number
  • increased B cell number
    • in the lymph node   (MGI Ref ID J:186080)
  • increased T cell number
    • in the lymph node   (MGI Ref ID J:186080)
    • increased CD4-positive, alpha beta T cell number
      • in the lymph node   (MGI Ref ID J:186080)
    • increased CD8-positive, alpha-beta T cell number
      • in the lymph node   (MGI Ref ID J:186080)
    • increased memory T cell number   (MGI Ref ID J:186080)
  • thymus hypoplasia   (MGI Ref ID J:186080)
  • endocrine/exocrine gland phenotype
  • decreased DN1 thymic pro-T cell number   (MGI Ref ID J:186080)
  • decreased DN4 thymocyte number   (MGI Ref ID J:186080)
  • thymus hypoplasia   (MGI Ref ID J:186080)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
T Cell Receptor Signaling Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Prkchtm1.2Gasc
Allele Name targeted mutation 1.2, Nicholas Gascoigne
Allele Type Targeted (Null/Knockout)
Strain of OriginB6.Cg-Thy1
Gene Symbol and Name Prkch, protein kinase C, eta
Chromosome 12
Gene Common Name(s) PKC-L; PKCL; PRKCL; Pkch; nPKC-eta;
Molecular Note A targeting vector was designed to insert a loxP site, followed by a frt-flanked neomycin resistance (neo) cassette, upstream of exon 2 and a second loxP site downstream of exon 2. Flp-mediated recombination removed the neo cassette while cre-mediated recombination remvoed exon 2. [MGI Ref ID J:186080]

Genotyping

Genotyping Information

Genotyping Protocols

Prkchtm1.2Gasc,

SEPARATED MELT


Prkchtm1.2Gasc, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Prkchtm1.2Gasc related

Fu G; Hu J; Niederberger-Magnenat N; Rybakin V; Casas J; Yachi PP; Feldstein S; Ma B; Hoerter JA; Ampudia J; Rigaud S; Lambolez F; Gavin AL; Sauer K; Cheroutre H; Gascoigne NR. 2011. Protein kinase C eta is required for T cell activation and homeostatic proliferation. Sci Signal 4(202):ra84. [PubMed: 22155788]  [MGI Ref ID J:186080]

Kong KF; Fu G; Zhang Y; Yokosuka T; Casas J; Canonigo-Balancio AJ; Becart S; Kim G; Yates JR 3rd; Kronenberg M; Saito T; Gascoigne NR; Altman A. 2014. Protein kinase C-eta controls CTLA-4-mediated regulatory T cell function. Nat Immunol 15(5):465-72. [PubMed: 24705298]  [MGI Ref ID J:209959]

Nabekura T; Kanaya M; Shibuya A; Fu G; Gascoigne NR; Lanier LL. 2014. Costimulatory molecule DNAM-1 is essential for optimal differentiation of memory natural killer cells during mouse cytomegalovirus infection. Immunity 40(2):225-34. [PubMed: 24440149]  [MGI Ref ID J:209371]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice may be bred together.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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