Strain Name:

B6N.129-Gria1tm2Rsp/J

Stock Number:

019012

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Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
GluA1 floxed exon 11 mice allow deletion of the sequences encoding the glutamate receptor transmembrane domain ion channel pore in cells/tissues expressing Cre-recombinase. These mice are useful in applications related to the study of behavioral, social and cognitive abnormalities, hippocampal synaptic transmission/plasticity, nociception, as well as neuropsychiatric disorders such as schizophrenia and depression/mania.

Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Mating SystemHomozygote x Homozygote         (Female x Male)   13-NOV-13
Specieslaboratory mouse
GenerationN7pN1+N1 (01-MAR-13)
Generation Definitions
 
Donating Investigator Rolf Sprengel,   Max Planck Institute for Medical Res

Description
The GluA1fl floxed allele (also called GluR1flox, GluR-A2lox, or AQ-2lox allele) has loxP sites flanking exon 11. Homozygous mice are viable and fertile. When bred to mice that express Cre recombinase, the resulting offspring will have the glutamate receptor transmembrane domain ion channel pore deleted in cre-expressing tissues. These GluA1fl mice may be useful in generating tissue-specific AMPA-type glutamate receptor deletions. Specific examples are described below.

When GluA1fl mice are bred to a strain expressing Cre recombinase in germ-line or embryonic tissues, the resulting mice are useful in studying the pan deletion of glutamate receptor function. GluA1 knockout mice are also distributed from The Jackson Laboratory Repository as Stock No. 019011.

When GluA1fl mice are bred to a strain with Cre recombinase in parvalbumin-expressing cells (see Stock Nos. 012358, 010777, 008069), the resulting mice allow studying interneurons and hippocampus function.

When GluA1fl mice are bred to a strain with Cre recombinase in dopamine neurotransmitter transporter-expressing cells (see Stock Nos. 016583 or 006660), the resulting mice allow studying dopaminergic neurons.

When GluA1fl mice are bred to a strain with Cre recombinase in Mnx1-expressing cells (HB9cre; Stock No 006600), the resulting mice allow neurodevelopmental studies of homeobox genes, motor neurons, and a subpopulation of spinal cord interneurons.

When GluA1fl mice are bred to a strain with tamoxifen-inducible Cre recombinase expression in glial high affinity glutamate transporter-expressing cells (see GLAST-CreER; Stock No 012586), the resulting mice allow neurodevelopmental studies of glia and neural progenitor cells.

Development
A targeting vector was designed to insert a loxP site upstream of exon 11, and a loxP-flanked neo cassette (from ploxpneo3) downstream of exon 11 of the glutamate receptor, ionotropic, AMPA1 [alpha 1] gene (Gria1; GluR-A, GluA1). This loxP::exon11::loxP::neo::loxP construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells with the GluR-Aneo allele (also called 3loxP allele) were then transiently transfected with a Cre recombinase-expressing plasmid. The resulting ES cells with the GluR-Afl genotype (exon 11 flanked by loxP sites and the neo selection cassette removed) were injected into recipient blastocysts. Chimeric mice were bred with C57BL/6 mice to establish the GluR-Afl colony. The donating investigator reports that GluR-Afl mice were subsequently backcrossed to C57BL/6NCrl mice for seven generations prior to sending to The Jackson Laboratory Repository in 2012. Upon arrival, mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for at least one generation.

Control Information

  Control
   005304 C57BL/6NJ
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Gria1
008892   B6.129-Gria1tm1Rlh/J
012614   B6.129-Gria1tm2Rlh/J
012612   B6.129-Gria1tm5Rlh/J
012613   B6.129-Gria1tm6Rlh/J
019011   B6N.129-Gria1tm1Rsp/J
View Strains carrying other alleles of Gria1     (5 strains)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Gria1tm2Rsp/Gria1tm2Rsp

        involves: 129S1/Sv * 129X1/SvJ   (conditional)
  • nervous system phenotype
  • abnormal miniature excitatory postsynaptic currents
    • reduced amplitude with faster decay in cre-infected neurons   (MGI Ref ID J:197912)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Behavioral and Learning Defects
      high anxiety
Channel and Transporter Defects
      calcium: glutamate receptor
Cortical Defects
Cre-lox System
      loxP-flanked Sequences
Neurotransmitter Receptor and Synaptic Vesicle Defects
Receptor Defects
      glutamate receptor: ionotropic

Research Tools
Cardiovascular Research
      Cre-lox System
Cre-lox System
      loxP-flanked Sequences
Developmental Biology Research
      Cre-lox System
Diabetes and Obesity Research
      loxP
Genetics Research
      Mutagenesis and Transgenesis: Cre-lox System
Neurobiology Research
Reproductive Biology Research
      Cre-lox System

Sensorineural Research
Nociception

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Gria1tm2Rsp
Allele Name targeted mutation 2, Rolf Sprengel
Allele Type Targeted (Floxed/Frt)
Common Name(s) GRIA1fl; GluR-A2lox; GluR1flox;
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name Gria1, glutamate receptor, ionotropic, AMPA1 (alpha 1)
Chromosome 11
Gene Common Name(s) 2900051M01Rik; AI853806; GLUH1; GLUR1; GLURA; Glr-1; Glr1; GluA1; GluR-A; Glur-1; Glur1; HBGR1; HIPA1; RIKEN cDNA 2900051M01 gene; expressed sequence AI853806; glutamate receptor 1; glutamate receptor 1 (alpha 1);
Molecular Note A floxed neo cassette was inserted 700 bp into exon 11 along with an additional loxP site inserted upstream of exon 10. This allele was used to generate Gria1tm1Rsp by transfecting targeted ES cells with a cre-expressing vector (pMC-cre). [MGI Ref ID J:55695]

Genotyping

Genotyping Information

Genotyping Protocols

Gria1tm2Rsp, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Emamian ES; Hall D; Birnbaum MJ; Karayiorgou M; Gogos JA. 2004. Convergent evidence for impaired AKT1-GSK3beta signaling in schizophrenia. Nat Genet 36(2):131-7. [PubMed: 14745448]  [MGI Ref ID J:185487]

Engblom D; Bilbao A; Sanchis-Segura C; Dahan L; Perreau-Lenz S; Balland B; Parkitna JR; Lujan R; Halbout B; Mameli M; Parlato R; Sprengel R; Luscher C; Schutz G; Spanagel R. 2008. Glutamate receptors on dopamine neurons control the persistence of cocaine seeking. Neuron 59(3):497-508. [PubMed: 18701074]  [MGI Ref ID J:139693]

Fuchs EC; Zivkovic AR; Cunningham MO; Middleton S; Lebeau FE; Bannerman DM; Rozov A; Whittington MA; Traub RD; Rawlins JN; Monyer H. 2007. Recruitment of parvalbumin-positive interneurons determines hippocampal function and associated behavior. Neuron 53(4):591-604. [PubMed: 17296559]  [MGI Ref ID J:136763]

Zamanillo D; Sprengel R; Hvalby O; Jensen V; Burnashev N; Rozov A; Kaiser KM; Koster HJ; Borchardt T; Worley P; Lubke J; Frotscher M; Kelly PH; Sommer B; Andersen P; Seeburg PH; Sakmann B. 1999. Importance of AMPA receptors for hippocampal synaptic plasticity but not for spatial learning [see comments] Science 284(5421):1805-11. [PubMed: 10364547]  [MGI Ref ID J:55695]

Zhang L; Schessl J; Werner M; Bonnemann C; Xiong G; Mojsilovic-Petrovic J; Zhou W; Cohen A; Seeburg P; Misawa H; Jayaram A; Personius K; Hollmann M; Sprengel R; Kalb R. 2008. Role of GluR1 in activity-dependent motor system development. J Neurosci 28(40):9953-68. [PubMed: 18829953]  [MGI Ref ID J:141815]

Additional References

Gria1tm2Rsp related

Freudenberg F; Marx V; Mack V; Layer LE; Klugmann M; Seeburg PH; Sprengel R; Celikel T. 2013. GluA1 and its PDZ-interaction: a role in experience-dependent behavioral plasticity in the forced swim test. Neurobiol Dis 52:160-7. [PubMed: 23262314]  [MGI Ref ID J:197650]

Gangadharan V; Wang R; Ulzhofer B; Luo C; Bardoni R; Bali KK; Agarwal N; Tegeder I; Hildebrandt U; Nagy GG; Todd AJ; Ghirri A; Haussler A; Sprengel R; Seeburg PH; MacDermott AB; Lewin GR; Kuner R. 2011. Peripheral calcium-permeable AMPA receptors regulate chronic inflammatory pain in mice. J Clin Invest 121(4):1608-23. [PubMed: 21383497]  [MGI Ref ID J:172020]

Herring BE; Shi Y; Suh YH; Zheng CY; Blankenship SM; Roche KW; Nicoll RA. 2013. Cornichon proteins determine the subunit composition of synaptic AMPA receptors. Neuron 77(6):1083-96. [PubMed: 23522044]  [MGI Ref ID J:197912]

Lu W; Isozaki K; Roche KW; Nicoll RA. 2010. Synaptic targeting of AMPA receptors is regulated by a CaMKII site in the first intracellular loop of GluA1. Proc Natl Acad Sci U S A 107(51):22266-71. [PubMed: 21135237]  [MGI Ref ID J:167298]

Lu W; Shi Y; Jackson AC; Bjorgan K; During MJ; Sprengel R; Seeburg PH; Nicoll RA. 2009. Subunit composition of synaptic AMPA receptors revealed by a single-cell genetic approach. Neuron 62(2):254-68. [PubMed: 19409270]  [MGI Ref ID J:155070]

Racz A; Ponomarenko AA; Fuchs EC; Monyer H. 2009. Augmented hippocampal ripple oscillations in mice with reduced fast excitation onto parvalbumin-positive cells. J Neurosci 29(8):2563-8. [PubMed: 19244531]  [MGI Ref ID J:145942]

Saab AS; Neumeyer A; Jahn HM; Cupido A; Simek AA; Boele HJ; Scheller A; Le Meur K; Gotz M; Monyer H; Sprengel R; Rubio ME; Deitmer JW; De Zeeuw CI; Kirchhoff F. 2012. Bergmann glial AMPA receptors are required for fine motor coordination. Science 337(6095):749-53. [PubMed: 22767895]  [MGI Ref ID J:186487]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           FGB27

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice may be bred together.
Mating SystemHomozygote x Homozygote         (Female x Male)   13-NOV-13
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHomozygous for Gria1tm2Rsp  
Price per Pair (US dollars $)Pair Genotype
$464.00Homozygous for Gria1tm2Rsp x Homozygous for Gria1tm2Rsp  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHomozygous for Gria1tm2Rsp  
Price per Pair (US dollars $)Pair Genotype
$603.20Homozygous for Gria1tm2Rsp x Homozygous for Gria1tm2Rsp  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Control Information

  Control
   005304 C57BL/6NJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

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