|The GluA1fl floxed exon 11 allele allows deletion of the sequences encoding the glutamate receptor transmembrane domain ion channel pore in cells/tissues expressing Cre-recombinase. These mice are useful in applications related to the study of behavioral, social and cognitive abnormalities, hippocampal synaptic transmission/plasticity, nociception, as well as neuropsychiatric disorders such as schizophrenia and depression/mania.|
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation +pN1
Donating Investigator Rolf Sprengel, Max Planck Institute for Medical Res
The GluA1fl floxed allele (also called GluR1flox, GluR-A2lox, or AQ-2lox allele) has loxP sites flanking exon 11. Homozygous mice are viable and fertile. When bred to mice that express Cre recombinase, the resulting offspring will have the glutamate receptor transmembrane domain ion channel pore deleted in cre-expressing tissues. These GluA1fl mice may be useful in generating tissue-specific AMPA-type glutamate receptor deletions. Specific examples are described below.
When GluA1fl mice are bred to a strain expressing Cre recombinase in germ-line or embryonic tissues, the resulting mice are useful in studying the pan deletion of glutamate receptor function. GluA1 knockout mice are also distributed from The Jackson Laboratory Repository as Stock No. 019011.
When GluA1fl mice are bred to a strain with Cre recombinase in parvalbumin-expressing cells (see Stock Nos. 012358, 010777, 008069), the resulting mice allow studying interneurons and hippocampus function.
When GluA1fl mice are bred to a strain with Cre recombinase in dopamine neurotransmitter transporter-expressing cells (see Stock Nos. 016583 or 006660), the resulting mice allow studying dopaminergic neurons.
When GluA1fl mice are bred to a strain with Cre recombinase in Mnx1-expressing cells (HB9cre; Stock No 006600), the resulting mice allow neurodevelopmental studies of homeobox genes, motor neurons, and a subpopulation of spinal cord interneurons.
When GluA1fl mice are bred to a strain with tamoxifen-inducible Cre recombinase expression in glial high affinity glutamate transporter-expressing cells (see GLAST-CreER; Stock No 012586), the resulting mice allow neurodevelopmental studies of glia and neural progenitor cells.
A targeting vector was designed to insert a loxP site upstream of exon 11, and a loxP-flanked neo cassette (from ploxpneo3) downstream of exon 11 of the glutamate receptor, ionotropic, AMPA1 [alpha 1] gene (Gria1; GluR-A, GluA1). This loxP::exon11::loxP::neo::loxP construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells with the GluR-Aneo allele (also called 3loxP allele) were then transiently transfected with a Cre recombinase-expressing plasmid. The resulting ES cells with the GluR-Afl genotype (exon 11 flanked by loxP sites and the neo selection cassette removed) were injected into recipient blastocysts. Chimeric mice were bred with C57BL/6 mice to establish the GluR-Afl colony. The donating investigator reports that GluR-Afl mice were subsequently backcrossed to C57BL/6NCrl mice for seven generations prior to sending to The Jackson Laboratory Repository in 2012. Upon arrival, mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for at least one generation.
|Considerations for Choosing Controls|
Strains carrying other alleles of Gria1
024420 B6.129(Cg)-Gria1tm4Rlh/J 008892 B6.129-Gria1tm1Rlh/J 012614 B6.129-Gria1tm2Rlh/J 012612 B6.129-Gria1tm5Rlh/J 012613 B6.129-Gria1tm6Rlh/J 024418 B6.129S6-Gria1tm7Rlh/J 019011 B6N.129-Gria1tm1Rsp/J 024422 C57BL/6-Gria1tm3Rlh/JView Strains carrying other alleles of Gria1 (8 strains)
View Mammalian Phenotype TermsMammalian Phenotype Terms provided by MGIassigned by genotype
The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.
Gria1tm2Rsp/Gria1tm2Rspinvolves: 129S1/Sv * 129X1/SvJ (conditional)
- nervous system phenotype
- abnormal miniature excitatory postsynaptic currents
- reduced amplitude with faster decay in cre-infected neurons (MGI Ref ID J:197912)View Research ApplicationsResearch ApplicationsThis mouse can be used to support research in many areas including:
Behavioral and Learning Defects
Channel and Transporter Defects
calcium: glutamate receptor
Neurotransmitter Receptor and Synaptic Vesicle Defects
glutamate receptor: ionotropic
Developmental Biology Research
Diabetes and Obesity Research
Mutagenesis and Transgenesis: Cre-lox System
Reproductive Biology Research
|Allele Name||targeted mutation 2, Rolf Sprengel|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Common Name(s)||GRIA1fl; GluR-A2lox; GluR1flox;|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl<+>|
|Gene Symbol and Name||Gria1, glutamate receptor, ionotropic, AMPA1 (alpha 1)|
|Gene Common Name(s)||2900051M01Rik; AI853806; GLUH1; GLUR1; GLURA; Glr-1; Glr1; GluA1; GluR-A; Glur-1; Glur1; HBGR1; HIPA1; RIKEN cDNA 2900051M01 gene; expressed sequence AI853806; glutamate receptor 1; glutamate receptor 1 (alpha 1);|
|Molecular Note||A floxed neo cassette was inserted 700 bp into exon 11 along with an additional loxP site inserted upstream of exon 10. This allele was used to generate Gria1tm1Rsp by transfecting targeted ES cells with a cre-expressing vector (pMC-cre). [MGI Ref ID J:55695]|
Emamian ES; Hall D; Birnbaum MJ; Karayiorgou M; Gogos JA. 2004. Convergent evidence for impaired AKT1-GSK3beta signaling in schizophrenia. Nat Genet 36(2):131-7. [PubMed: 14745448] [MGI Ref ID J:185487]
Engblom D; Bilbao A; Sanchis-Segura C; Dahan L; Perreau-Lenz S; Balland B; Parkitna JR; Lujan R; Halbout B; Mameli M; Parlato R; Sprengel R; Luscher C; Schutz G; Spanagel R. 2008. Glutamate receptors on dopamine neurons control the persistence of cocaine seeking. Neuron 59(3):497-508. [PubMed: 18701074] [MGI Ref ID J:139693]
Fuchs EC; Zivkovic AR; Cunningham MO; Middleton S; Lebeau FE; Bannerman DM; Rozov A; Whittington MA; Traub RD; Rawlins JN; Monyer H. 2007. Recruitment of parvalbumin-positive interneurons determines hippocampal function and associated behavior. Neuron 53(4):591-604. [PubMed: 17296559] [MGI Ref ID J:136763]
Zamanillo D; Sprengel R; Hvalby O; Jensen V; Burnashev N; Rozov A; Kaiser KM; Koster HJ; Borchardt T; Worley P; Lubke J; Frotscher M; Kelly PH; Sommer B; Andersen P; Seeburg PH; Sakmann B. 1999. Importance of AMPA receptors for hippocampal synaptic plasticity but not for spatial learning [see comments] Science 284(5421):1805-11. [PubMed: 10364547] [MGI Ref ID J:55695]
Zhang L; Schessl J; Werner M; Bonnemann C; Xiong G; Mojsilovic-Petrovic J; Zhou W; Cohen A; Seeburg P; Misawa H; Jayaram A; Personius K; Hollmann M; Sprengel R; Kalb R. 2008. Role of GluR1 in activity-dependent motor system development. J Neurosci 28(40):9953-68. [PubMed: 18829953] [MGI Ref ID J:141815]
Freudenberg F; Marx V; Mack V; Layer LE; Klugmann M; Seeburg PH; Sprengel R; Celikel T. 2013. GluA1 and its PDZ-interaction: a role in experience-dependent behavioral plasticity in the forced swim test. Neurobiol Dis 52:160-7. [PubMed: 23262314] [MGI Ref ID J:197650]
Gangadharan V; Wang R; Ulzhofer B; Luo C; Bardoni R; Bali KK; Agarwal N; Tegeder I; Hildebrandt U; Nagy GG; Todd AJ; Ghirri A; Haussler A; Sprengel R; Seeburg PH; MacDermott AB; Lewin GR; Kuner R. 2011. Peripheral calcium-permeable AMPA receptors regulate chronic inflammatory pain in mice. J Clin Invest 121(4):1608-23. [PubMed: 21383497] [MGI Ref ID J:172020]
Herring BE; Shi Y; Suh YH; Zheng CY; Blankenship SM; Roche KW; Nicoll RA. 2013. Cornichon proteins determine the subunit composition of synaptic AMPA receptors. Neuron 77(6):1083-96. [PubMed: 23522044] [MGI Ref ID J:197912]
Lu W; Isozaki K; Roche KW; Nicoll RA. 2010. Synaptic targeting of AMPA receptors is regulated by a CaMKII site in the first intracellular loop of GluA1. Proc Natl Acad Sci U S A 107(51):22266-71. [PubMed: 21135237] [MGI Ref ID J:167298]
Lu W; Shi Y; Jackson AC; Bjorgan K; During MJ; Sprengel R; Seeburg PH; Nicoll RA. 2009. Subunit composition of synaptic AMPA receptors revealed by a single-cell genetic approach. Neuron 62(2):254-68. [PubMed: 19409270] [MGI Ref ID J:155070]
Racz A; Ponomarenko AA; Fuchs EC; Monyer H. 2009. Augmented hippocampal ripple oscillations in mice with reduced fast excitation onto parvalbumin-positive cells. J Neurosci 29(8):2563-8. [PubMed: 19244531] [MGI Ref ID J:145942]
Saab AS; Neumeyer A; Jahn HM; Cupido A; Simek AA; Boele HJ; Scheller A; Le Meur K; Gotz M; Monyer H; Sprengel R; Rubio ME; Deitmer JW; De Zeeuw CI; Kirchhoff F. 2012. Bergmann glial AMPA receptors are required for fine motor coordination. Science 337(6095):749-53. [PubMed: 22767895] [MGI Ref ID J:186487]
Animal Health ReportsProduction of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.
Breeding & Husbandry When maintaining a live colony, homozygous mice may be bred together.
|Pricing for USA, Canada and Mexico shipping destinations|
Cryopreserved Mice - Ready for Recovery
Price (US dollars $) Cryorecovery* $2140.00
At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
|Pricing for International shipping destinations|
Cryopreserved Mice - Ready for Recovery
Price (US dollars $) Cryorecovery* $2782.00
Cryorecovery - Standard.
Progeny testing is not required.
|Considerations for Choosing Controls|
|Control Pricing Information for Genetically Engineered Mutant Strains.|
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