Strain Name:

FVB.Cg-Tg(tetO-KLF4)32831Rup/Mmjax

Availability:

Repository- Live     Available at the JAX MMRRC

Please refer to the Mutant Mouse Regional Resource Center (MMRRC) for information about FVB.Cg-Tg(tetO-KLF4)32831Rup/Mmjax Stock Number 036730.
These transgenic mice express human KLF4, Kruppel-like factor 4, under the direction of the tetracycline operator promoter (tetO). Following DOX induction, bitransgenic mice exhibit hyperkeratosis, atrophy of sebaceous glands, cystic degeneration of hair follicles and hyperplasia. This strain may be useful for studying squamous cell carcinoma (SCC).

Description

Strain Information

Type Congenic; Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
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Mating SystemNoncarrier x Hemizygote         (Female x Male)   19-SEP-13
Mating SystemHemizygote x Noncarrier         (Female x Male)   19-SEP-13
Specieslaboratory mouse
 
Donating Investigator Michael Ruppert,   West Virginia University

Description
These transgenic mice express human KLF4, Kruppel-like factor 4, under the direction of the tetracycline operator promoter (tetO). Mice hemizygous for the transgenic insert are viable and fertile. It is not known if homozygotes are viable. When mated to mice carrying K14-rtTA (also known as Tg(KRT14-rtTA)208Jek)), KLF4 expression is directed to basal keratinocytes and regulated with tetracycline or its analog doxycycline (DOX). Following DOX induction, bitransgenic mice exhibit hyperkeratosis, atrophy of sebaceous glands, cystic degeneration of hair follicles and hyperplasia. This strain may be useful for studying squamous cell carcinoma (SCC).

Development
A 4.4 kB transgenic construct containing a tetracycline responsive element (TRE or tetO), the minimal human cytomegalovirus promoter, and the protein coding region of the wildtype human KLF4, Kruppel-like factor 4 was injected into fertilized (C57BL/6 x SJL)F2 mouse eggs. Founder line 32831 was subsequently established. Founders were bred to (C57BL/6 x SJL)F2 mice and then to FVB/NJ for at least 8 generations. Upon arrival at The Jackson Laboratory Repository, the mice were bred with FVB/NJ mice for at least one generation to establish the colony.

Control Information

  Control
   001800 FVB/NJ
 
  Considerations for Choosing Controls

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View Strains carrying other alleles of tetO     (137 strains)

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Research Tools
Cancer Research
      Tetop Tet System

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(tetO-KLF4)32831Rup
Allele Name transgene insertion 32831, J Michael Ruppert
Allele Type Transgenic (Inducible, Inserted expressed sequence)
Common Name(s) TRE-KLF4; TRE-KLF432831;
Mutation Made By Michael Ruppert,   West Virginia University
Strain of Origin(C57BL/6 x SJL)F2
Expressed Gene KLF4, Kruppel-like factor 4 (gut), human
Promoter tetO, tet operator,
Molecular Note The protein-coding region of the human Kruppel-like factor gene KLF4 was ligated to a vector fragment containing a tetracycline-responsive element and the minimal human cytomegalovirus promoter to produce the transgene construct. The 4.4 kb fragment was injected into (C57BL/6 x SJL)F2 ova. [MGI Ref ID J:96886]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(tetO-KLF4)32831Rup, Melt Curve Analysis
Tg(tetO-KLF4)32831Rup, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Foster KW; Liu Z; Nail CD; Li X; Fitzgerald TJ; Bailey SK; Frost AR; Louro ID; Townes TM; Paterson AJ; Kudlow JE; Lobo-Ruppert SM; Ruppert JM. 2005. Induction of KLF4 in basal keratinocytes blocks the proliferation-differentiation switch and initiates squamous epithelial dysplasia. Oncogene 24(9):1491-500. [PubMed: 15674344]  [MGI Ref ID J:96886]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & HusbandryWhile maintaining a live colony, these mice are bred as hemizygotes. The donating investigator has not attempted to make the colony homozygous.
Mating SystemNoncarrier x Hemizygote         (Female x Male)   19-SEP-13
Hemizygote x Noncarrier         (Female x Male)   19-SEP-13

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