Description

Strain Information

Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Noncarrier x Hemizygote         (Female x Male)   27-DEC-12 Mating System Hemizygote x Noncarrier         (Female x Male)   27-DEC-12 Species laboratory mouse Generation ?pN1+ (08-FEB-13) Generation Definitions   Donating Investigator Jeffrey W. Pollard,   Albert Einstein College of Medicine

Description
These Tg(Csf1r-Mer-iCre-Mer)1Jwp transgenic mice express a Cre recombinase/mutant murine estrogen receptor double-fusion protein under the direction of the mouse Csf1r, colony stimulating factor 1 receptor, promoter. Tamoxifen-inducible cre activity is detected in bone-marrow-derived macrophages and yolk sac macrophages. When these Tg(Csf1r-Mer-iCre-Mer)1Jwp mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in Csf1r-expressing cells; making them useful for studying inflammation, metastasis and tracking myeloid lineage cells.

The MerCreMer double fusion protein consists of Cre recombinase flanked on each end with a mutated murine estrogen receptor (mer) ligand binding domain (amino acids 281-599, G525R); which does not bind its natural ligand (17'-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT or tamoxifen) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, MerCreMer can only gain access to the nuclear compartment after exposure to tamoxifen. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be co-administered.

Development
A transgenic construct containing tandem copies of icre/Esr1*, or Mer-icre-Mer, under the control of the mouse Csf1r, colony stimulating factor 1 receptor, promoter, was injected into fertilized FVB/N mouse eggs. Founder line 1 was subsequently established. Upon arrival at The Jackson Laboratory, the mice were crossed to FVB/NJ (Stock No. 001800) at least once to establish the colony.

Control Information

	Control
	Noncarrier
	001800 FVB/NJ
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Csf1r

021212	B6.Cg-Csf1rtm1Jwp/J
018549	B6N.Cg-Tg(Csf1r-EGFP)1Hume/J
005070	C57BL/6-Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck/J
021024	FVB-Tg(Csf1r-icre)1Jwp/J

View Strains carrying other alleles of Csf1r     (4 strains)

Phenotype

Phenotype Information

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Research Tools
Cre-lox System
      Cre Recombinase Expression: Inducible

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Tg(Csf1r-cre/Esr1*)1Jwp
Allele Name		transgene insertion 1, Jeffrey W Pollard
Allele Type		Transgenic (Cre/Flp)
Common Name(s)		tg(Csf1r-Mer-iCre-Mer)1Jwp;
Strain of Origin		FVB/N
Site of Expression		Tamoxifen-inducible cre activity is detected in bone-marrow-derived macrophages and yolk sac macrophages.
Expressed Gene		cre/Esr1, Cre recombinase and estrogen receptor 1 fusion gene,
Promoter		Csf1r, colony stimulating factor 1 receptor, mouse, laboratory
Driver Note		Csf1r
Inducible Note		induced by tamoxifen
Molecular Note		A transgene construct was generated in which the Csf1r promoter was used to drive expression of icre fused to 2 tamoxifen-inducible mutated estrogen receptors (Mer-icre-Mer or icre/Esr1*). Line 1 was used in the primary reference. [MGI Ref ID J:186831]
Gene Symbol and Name		Tg(Csf1r-cre/Esr1*)1Jwp, transgene insertion 1, Jeffrey W Pollard
Chromosome		UN

Genotyping

Genotyping Information

Genotyping Protocols

Generic iCre, Melt Curve Analysis
Generic iCre, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Qian BZ; Li J; Zhang H; Kitamura T; Zhang J; Campion LR; Kaiser EA; Snyder LA; Pollard JW. 2011. CCL2 recruits inflammatory monocytes to facilitate breast-tumour metastasis. Nature 475(7355):222-5. [PubMed: 21654748]  [MGI Ref ID J:186831]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX18

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, hemizygous mice may be bred together, to wildtype siblings, or to FVB/NJ inbred mice (Stock No. 001800). The Donating Investigator reports that homozygotes are viable.
Mating System	Noncarrier x Hemizygote         (Female x Male)   27-DEC-12
	Hemizygote x Noncarrier         (Female x Male)   27-DEC-12
Diet Information	LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Control Information

	Control
	Noncarrier
	001800 FVB/NJ
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering Information
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Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Mice are subject to US Patent 6040430.

Contact information

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phone:	207-288-6470
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JAX^® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

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