Strain Name:

FVB-Tg(Csf1r-cre/Esr1*)1Jwp/J

Stock Number:

019098

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Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
These Tg(Csf1r-Mer-iCre-Mer)1Jwp transgenic mice express the tamoxifen-inducible MerCreMer fusion protein under control of the macrophage specific mouse Csf1r, colony stimulating factor 1 receptor, promoter.

Description

Strain Information

Type Coisogenic; Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
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Mating SystemNoncarrier x Hemizygote         (Female x Male)   27-DEC-12
Mating SystemHemizygote x Noncarrier         (Female x Male)   27-DEC-12
Specieslaboratory mouse
Generation?pN1+ (08-FEB-13)
Generation Definitions
 
Donating Investigator Jeffrey W. Pollard,   Albert Einstein College of Medicine

Description
These Tg(Csf1r-Mer-iCre-Mer)1Jwp transgenic mice express a Cre recombinase/mutant murine estrogen receptor double-fusion protein under the direction of the mouse Csf1r, colony stimulating factor 1 receptor, promoter. Tamoxifen-inducible cre activity is detected in bone-marrow-derived macrophages and yolk sac macrophages. When these Tg(Csf1r-Mer-iCre-Mer)1Jwp mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in Csf1r-expressing cells; making them useful for studying inflammation, metastasis and tracking myeloid lineage cells.

The MerCreMer double fusion protein consists of Cre recombinase flanked on each end with a mutated murine estrogen receptor (mer) ligand binding domain (amino acids 281-599, G525R); which does not bind its natural ligand (17'-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT or tamoxifen) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, MerCreMer can only gain access to the nuclear compartment after exposure to tamoxifen. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be co-administered.

Development
A transgenic construct containing tandem copies of icre/Esr1*, or Mer-icre-Mer, under the control of the mouse Csf1r, colony stimulating factor 1 receptor, promoter, was injected into fertilized FVB/N mouse eggs. Founder line 1 was subsequently established. Upon arrival at The Jackson Laboratory, the mice were crossed to FVB/NJ (Stock No. 001800) at least once to establish the colony.

Control Information

  Control
   Noncarrier
   001800 FVB/NJ
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of Csf1r     (5 strains)

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Research Tools
Cre-lox System
      Cre Recombinase Expression: Inducible

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(Csf1r-cre/Esr1*)1Jwp
Allele Name transgene insertion 1, Jeffrey W Pollard
Allele Type Transgenic (Cre/Flp)
Common Name(s) tg(Csf1r-Mer-iCre-Mer)1Jwp;
Strain of OriginFVB/N
Site of ExpressionTamoxifen-inducible cre activity is detected in bone-marrow-derived macrophages and yolk sac macrophages.
Expressed Gene cre/Esr1, Cre recombinase and estrogen receptor 1 fusion gene,
Promoter Csf1r, colony stimulating factor 1 receptor, mouse, laboratory
Driver Note Csf1r
Inducible Note induced by tamoxifen
Molecular Note A transgene construct was generated in which the Csf1r promoter was used to drive expression of icre fused to 2 tamoxifen-inducible mutated estrogen receptors (Mer-icre-Mer or icre/Esr1*). Line 1 was used in the primary reference. [MGI Ref ID J:186831]
 
Gene Symbol and Name Tg(Csf1r-cre/Esr1*)1Jwp, transgene insertion 1, Jeffrey W Pollard
Chromosome UN
 

Genotyping

Genotyping Information

Genotyping Protocols

Generic iCre, Melt Curve Analysis
iCre QPCR, QPCR
Generic iCre, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Qian BZ; Li J; Zhang H; Kitamura T; Zhang J; Campion LR; Kaiser EA; Snyder LA; Pollard JW. 2011. CCL2 recruits inflammatory monocytes to facilitate breast-tumour metastasis. Nature 475(7355):222-5. [PubMed: 21654748]  [MGI Ref ID J:186831]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX18

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, hemizygous mice may be bred together, to wildtype siblings, or to FVB/NJ inbred mice (Stock No. 001800). The Donating Investigator reports that homozygotes are viable.
Mating SystemNoncarrier x Hemizygote         (Female x Male)   27-DEC-12
Hemizygote x Noncarrier         (Female x Male)   27-DEC-12
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHemizygous for Tg(Csf1r-cre/Esr1*)1Jwp  
Price per Pair (US dollars $)Pair Genotype
$302.00Hemizygous for Tg(Csf1r-cre/Esr1*)1Jwp x Noncarrier  
$302.00Noncarrier x Hemizygous for Tg(Csf1r-cre/Esr1*)1Jwp  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHemizygous for Tg(Csf1r-cre/Esr1*)1Jwp  
Price per Pair (US dollars $)Pair Genotype
$392.60Hemizygous for Tg(Csf1r-cre/Esr1*)1Jwp x Noncarrier  
$392.60Noncarrier x Hemizygous for Tg(Csf1r-cre/Esr1*)1Jwp  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Control Information

  Control
   Noncarrier
   001800 FVB/NJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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- Mice are subject to US Patent 6040430.

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