Strain Name:

B6.129X1-Spo11tm1Mjn/J

Stock Number:

019117

Order this mouse

Availability:

Cryopreserved - Ready for recovery

Disruption of Spo11 (SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae)) leads to severe gonadal abnormalities in both males and females due to defective meiosis.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Generation30pN1
Generation Definitions
 
Donating Investigator Maria Jasin,   Memorial Sloan-Kettering Cancer Center
Donating Investigator Scott Keeney,   Memorial Sloan-Kettering Cancer Center

Description
Disruption of Spo11 (SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae)) leads to severe gonadal abnormalities in both males and females due to defective meiosis.

These mice carry a targeted deletion of exons 4-6, removing a putative catalytic tyrosine encoded in exon 5. Northern blot analysis indicates that the null allele does not express transcripts.

Gonads from homozygous adult males are significantly reduced in weight. Spermatocytes undergo apoptotic death during early prophase. Oocytes from homozygous females progress to the diplotene/dictyate stage in nearly normal numbers, but most die soon after birth. Homozygotes also demonstrate defects in meiocyte homologous chromosomes and are unable to initiate meiotic recombination. Axial elements appear to be normal, but homologous synapsis is impaired.

Heterozygotes are fully fertile, but homozygous males and females are not. Homozygotes are born at predicted Mendelian ratios, indicating their viability is not affected.

Development
A targeting vector containing a neomycin resistance cassette was used to replace exons 4 to 6. The construct was electroporated into 129X1/SvJ-derived embryonic stem (ES) cells (Genome Systems). Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to C57BL/6J for 29 generations by the donating lab.

Control Information

  Control
   +/+ from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Spo11tm1Mjn/Spo11tm1Mjn

        involves: 129X1/SvJ * C57BL/6
  • reproductive system phenotype
  • abnormal oogenesis
    • at E15.5 most oocytes appear to be in the leptotene or early zygotene stage   (MGI Ref ID J:65880)
    • at E16.5 most oocytes still have an early zygotene morphology with a few reaching an abnormal zygotene like stage with long axial elements in synapsis but with partner exchanges   (MGI Ref ID J:65880)
    • at E17.5 most nuclei are either in an abnormal zygotene like stage or have appear to be fully synapsed but lack Scp1 expression   (MGI Ref ID J:65880)
    • abnormal female meiosis
      • Dcm1/Rad51 foci are nearly absent suggesting mice are unable to initiate meiotic recombination   (MGI Ref ID J:65880)
      • oocytes display defects in synapsis   (MGI Ref ID J:65880)
    • decreased oocyte number
      • normal numbers of oogonia are present at birth but oocyte numbers decline rapidly after birth   (MGI Ref ID J:65880)
      • at P4 and P8 only 10% - 20% of the normal number of c-kit positive oocytes are seen   (MGI Ref ID J:65880)
      • at 8 weeks of age oocyte depletion is more pronounced   (MGI Ref ID J:65880)
  • abnormal ovary morphology
    • at 8 weeks of age ovarian dysgenesis is apparent   (MGI Ref ID J:65880)
    • abnormal primordial ovarian follicle morphology
      • at P13 the cortex of the ovary is nearly devoid of primordial follicles   (MGI Ref ID J:65880)
    • decreased mature ovarian follicle number
      • at 10 weeks of age only a few intermediate stage follicles and a single preovulatory follicle are seen   (MGI Ref ID J:65880)
    • impaired ovarian folliculogenesis
      • at P13, after the first cohort of oocytes is normally recruited to grow, the number of growing follicles is reduced 2 fold   (MGI Ref ID J:65880)
      • at 10 weeks of age only a few intermediate stage follicles and a single preovulatory follicle are seen   (MGI Ref ID J:65880)
    • small ovary
      • slightly smaller compared to wild-type mice   (MGI Ref ID J:65880)
  • abnormal spermatogenesis
    • increase in the incidence of apoptotic cells in the tubules   (MGI Ref ID J:65880)
    • abnormal male meiosis
      • few or no cells progress through meiosis   (MGI Ref ID J:65880)
      • Dcm1/Rad51 foci are nearly absent suggesting mice are unable to initiate meiotic recombination   (MGI Ref ID J:65880)
      • spermatocytes are seen with much longer axial elements and short synaptonemal complexes, with longer synaptonemal complexes but axial elements involved in synapsis with several partners, or without synaptonemal complexes   (MGI Ref ID J:65880)
      • spermatocyte nuclei with complete synapsis are not seen   (MGI Ref ID J:65880)
    • abnormal spermatid morphology
    • arrest of spermatogenesis
      • seminiferous tubules generally contain only spermatogonia and Sertoli cells or spermatogonia and Sertoli cells plus either early prophase spermatocytes or cells with highly condensed chromatin fragments   (MGI Ref ID J:65880)
      • no spermatocytes with pachytene or later morphology are seen   (MGI Ref ID J:65880)
    • azoospermia
      • absence of epididymal sperm   (MGI Ref ID J:65880)
  • decreased testis weight
    • at 10 and 14 weeks of age testis weights are 20% - 50% of wild-type or heterozygous littermates   (MGI Ref ID J:65880)
  • female infertility   (MGI Ref ID J:65880)
  • male infertility   (MGI Ref ID J:65880)
  • small seminiferous tubules   (MGI Ref ID J:65880)
  • immune system phenotype
  • *normal* immune system phenotype
    • despite expression patterns and the known role in double strand breaks in meiosis, no abnormalities in somatic hypermutation or class switch recombination are detected   (MGI Ref ID J:74754)
  • endocrine/exocrine gland phenotype
  • abnormal ovary morphology
    • at 8 weeks of age ovarian dysgenesis is apparent   (MGI Ref ID J:65880)
    • abnormal primordial ovarian follicle morphology
      • at P13 the cortex of the ovary is nearly devoid of primordial follicles   (MGI Ref ID J:65880)
    • decreased mature ovarian follicle number
      • at 10 weeks of age only a few intermediate stage follicles and a single preovulatory follicle are seen   (MGI Ref ID J:65880)
    • impaired ovarian folliculogenesis
      • at P13, after the first cohort of oocytes is normally recruited to grow, the number of growing follicles is reduced 2 fold   (MGI Ref ID J:65880)
      • at 10 weeks of age only a few intermediate stage follicles and a single preovulatory follicle are seen   (MGI Ref ID J:65880)
    • small ovary
      • slightly smaller compared to wild-type mice   (MGI Ref ID J:65880)
  • decreased testis weight
    • at 10 and 14 weeks of age testis weights are 20% - 50% of wild-type or heterozygous littermates   (MGI Ref ID J:65880)
  • small seminiferous tubules   (MGI Ref ID J:65880)

Spo11tm1Mjn/Spo11tm1Mjn

        involves: 129X1/SvJ * C57BL/6JOlaHsd
  • reproductive system phenotype
  • abnormal female meiosis
    • pseudo-sex-body-like formation is impaired compared to in wild-type cells   (MGI Ref ID J:173089)
  • arrest of spermatogenesis
    • spermatocytes are eliminated in stage-IV tubules unlike in wild-type mice   (MGI Ref ID J:173089)
  • decreased oocyte number
    • very few oocytes survive until 6 weeks unlike in wild-type mice   (MGI Ref ID J:173089)
  • oocyte degeneration
    • at P1, oocyte apoptosis rates are higher than in wild-type cells   (MGI Ref ID J:173089)

Spo11tm1Mjn/Spo11tm1Mjn

        involves: 129X1/SvJ
  • reproductive system phenotype
  • abnormal meiosis
    • a subset of unsynapsed chromosome axes are surrounded by H2AFX rich chromatin domains forming pseudo-sex bodies   (MGI Ref ID J:183982)
    • abnormal female meiosis
      • pseudo-sex body formation in oocytes is impaired   (MGI Ref ID J:192634)
  • decreased oocyte number   (MGI Ref ID J:192634)
    • much lower numbers at 6 weeks of age   (MGI Ref ID J:183982)
  • small ovary   (MGI Ref ID J:192634)
    • decreased ovary weight   (MGI Ref ID J:192634)
  • endocrine/exocrine gland phenotype
  • small ovary   (MGI Ref ID J:192634)
    • decreased ovary weight   (MGI Ref ID J:192634)

Spo11tm1Mjn/Spo11tm1Mjn

        involves: 129X1/SvJ * C57BL/6J
  • reproductive system phenotype
  • abnormal male meiosis
    • spermocyte exhibit neither early centromere-associations nor preferential initiation of synaptonemal complex formation from centromeres   (MGI Ref ID J:187173)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Reproductive Biology Research
Developmental Defects Affecting Gonads
Fertility Defects
Meiotic Nondisjunction Studies

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Spo11tm1Mjn
Allele Name targeted mutation 1, Maria Jasin
Allele Type Targeted (knock-out)
Common Name(s) Spo11-; Spo11tm1Sky; Spotm1M;
Strain of Origin129X1/SvJ
Gene Symbol and Name Spo11, SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae)
Chromosome 2
Gene Common Name(s) AI449549; CT35; SPATA43; TOPVIA; expressed sequence AI449549;
Molecular Note Exons 4-6 of this gene were replaced with a neomycin resistance cassette via homologous recombination thus deleting the putative catalytic tyrosine in exon 5. Northern blot assay failed to detect gene transcripts in testes of homozygous mutant males. [MGI Ref ID J:65880]

Genotyping

Genotyping Information

Genotyping Protocols

Spo11tm1Mjn ,

SEPARATED MELT


Spo11tm1Mjn, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Baudat F; Manova K; Yuen JP; Jasin M; Keeney S. 2000. Chromosome synapsis defects and sexually dimorphic meiotic progression in mice lacking spo11 Mol Cell 6(5):989-98. [PubMed: 11106739]  [MGI Ref ID J:65880]

Additional References

Spo11tm1Mjn related

Barchi M; Mahadevaiah S; Di Giacomo M; Baudat F; de Rooij DG; Burgoyne PS; Jasin M; Keeney S. 2005. Surveillance of different recombination defects in mouse spermatocytes yields distinct responses despite elimination at an identical developmental stage. Mol Cell Biol 25(16):7203-15. [PubMed: 16055729]  [MGI Ref ID J:133053]

Barchi M; Roig I; Di Giacomo M; de Rooij DG; Keeney S; Jasin M. 2008. ATM promotes the obligate XY crossover and both crossover control and chromosome axis integrity on autosomes. PLoS Genet 4(5):e1000076. [PubMed: 18497861]  [MGI Ref ID J:137071]

Biswas U; Wetzker C; Lange J; Christodoulou EG; Seifert M; Beyer A; Jessberger R. 2013. Meiotic cohesin SMC1beta provides prophase I centromeric cohesion and is required for multiple synapsis-associated functions. PLoS Genet 9(12):e1003985. [PubMed: 24385917]  [MGI Ref ID J:207501]

Blanco-Rodriguez J. 2012. Programmed phosphorylation of histone H2AX precedes a phase of DNA double-strand break-independent synapsis in mouse meiosis. Reproduction 144(6):699-712. [PubMed: 23035256]  [MGI Ref ID J:195900]

Bolcun-Filas E; Bannister LA; Barash A; Schimenti KJ; Hartford SA; Eppig JJ; Handel MA; Shen L; Schimenti JC. 2011. A-MYB (MYBL1) transcription factor is a master regulator of male meiosis. Development 138(15):3319-30. [PubMed: 21750041]  [MGI Ref ID J:175539]

Chicheportiche A; Bernardino-Sgherri J; de Massy B; Dutrillaux B. 2007. Characterization of Spo11-dependent and independent phospho-H2AX foci during meiotic prophase I in the male mouse. J Cell Sci 120(Pt 10):1733-42. [PubMed: 17456548]  [MGI Ref ID J:124198]

Cole F; Keeney S; Jasin M. 2010. Evolutionary conservation of meiotic DSB proteins: more than just Spo11. Genes Dev 24(12):1201-7. [PubMed: 20551169]  [MGI Ref ID J:161204]

Daniel K; Lange J; Hached K; Fu J; Anastassiadis K; Roig I; Cooke HJ; Stewart AF; Wassmann K; Jasin M; Keeney S; Toth A. 2011. Meiotic homologue alignment and its quality surveillance are controlled by mouse HORMAD1. Nat Cell Biol 13(5):599-610. [PubMed: 21478856]  [MGI Ref ID J:173089]

Di Giacomo M; Barchi M; Baudat F; Edelmann W; Keeney S; Jasin M. 2005. Distinct DNA-damage-dependent and -independent responses drive the loss of oocytes in recombination-defective mouse mutants. Proc Natl Acad Sci U S A 102(3):737-42. [PubMed: 15640358]  [MGI Ref ID J:96250]

Guillon H; Baudat F; Grey C; Liskay RM; de Massy B. 2005. Crossover and noncrossover pathways in mouse meiosis. Mol Cell 20(4):563-73. [PubMed: 16307920]  [MGI Ref ID J:104590]

Kauppi L; Barchi M; Baudat F; Romanienko PJ; Keeney S; Jasin M. 2011. Distinct properties of the XY pseudoautosomal region crucial for male meiosis. Science 331(6019):916-20. [PubMed: 21330546]  [MGI Ref ID J:169057]

Kauppi L; Barchi M; Lange J; Baudat F; Jasin M; Keeney S. 2013. Numerical constraints and feedback control of double-strand breaks in mouse meiosis. Genes Dev 27(8):873-86. [PubMed: 23599345]  [MGI Ref ID J:198453]

Klein U; Esposito G; Baudat F; Keeney S; Jasin M. 2002. Mice deficient for the type II topoisomerase-like DNA transesterase Spo11 show normal immunoglobulin somatic hypermutation and class switching. Eur J Immunol 32(2):316-21. [PubMed: 11807770]  [MGI Ref ID J:74754]

Kogo H; Tsutsumi M; Inagaki H; Ohye T; Kiyonari H; Kurahashi H. 2012. HORMAD2 is essential for synapsis surveillance during meiotic prophase via the recruitment of ATR activity. Genes Cells 17(11):897-912. [PubMed: 23039116]  [MGI Ref ID J:192634]

Kumar R; Bourbon HM; de Massy B. 2010. Functional conservation of Mei4 for meiotic DNA double-strand break formation from yeasts to mice. Genes Dev 24(12):1266-80. [PubMed: 20551173]  [MGI Ref ID J:161203]

Lange J; Pan J; Cole F; Thelen MP; Jasin M; Keeney S. 2011. ATM controls meiotic double-strand-break formation. Nature 479(7372):237-40. [PubMed: 22002603]  [MGI Ref ID J:178306]

Li X; Schimenti JC. 2007. Mouse Pachytene Checkpoint 2 (Trip13) Is Required for Completing Meiotic Recombination but Not Synapsis. PLoS Genet 3(8):e130. [PubMed: 17696610]  [MGI Ref ID J:123677]

Li XZ; Roy CK; Dong X; Bolcun-Filas E; Wang J; Han BW; Xu J; Moore MJ; Schimenti JC; Weng Z; Zamore PD. 2013. An ancient transcription factor initiates the burst of piRNA production during early meiosis in mouse testes. Mol Cell 50(1):67-81. [PubMed: 23523368]  [MGI Ref ID J:198228]

Liebe B; Petukhova G; Barchi M; Bellani M; Braselmann H; Nakano T; Pandita TK; Jasin M; Fornace A; Meistrich ML; Baarends WM; Schimenti J; de Lange T; Keeney S; Camerini-Otero RD; Scherthan H. 2006. Mutations that affect meiosis in male mice influence the dynamics of the mid-preleptotene and bouquet stages. Exp Cell Res 312(19):3768-81. [PubMed: 17010969]  [MGI Ref ID J:147391]

Mahadevaiah SK; Bourc'his D; de Rooij DG; Bestor TH; Turner JM; Burgoyne PS. 2008. Extensive meiotic asynapsis in mice antagonises meiotic silencing of unsynapsed chromatin and consequently disrupts meiotic sex chromosome inactivation. J Cell Biol 182(2):263-76. [PubMed: 18663141]  [MGI Ref ID J:139518]

Mahadevaiah SK; Turner JM; Baudat F; Rogakou EP; de Boer P; Blanco-Rodriguez J; Jasin M; Keeney S; Bonner WM; Burgoyne PS. 2001. Recombinational DNA double-strand breaks in mice precede synapsis. Nat Genet 27(3):271-6. [PubMed: 11242108]  [MGI Ref ID J:126641]

Paronetto MP; Messina V; Barchi M; Geremia R; Richard S; Sette C. 2011. Sam68 marks the transcriptionally active stages of spermatogenesis and modulates alternative splicing in male germ cells. Nucleic Acids Res 39(12):4961-74. [PubMed: 21355037]  [MGI Ref ID J:173800]

Qiao H; Chen JK; Reynolds A; Hoog C; Paddy M; Hunter N. 2012. Interplay between synaptonemal complex, homologous recombination, and centromeres during mammalian meiosis. PLoS Genet 8(6):e1002790. [PubMed: 22761591]  [MGI Ref ID J:187173]

Qiao H; Prasada Rao HB; Yang Y; Fong JH; Cloutier JM; Deacon DC; Nagel KE; Swartz RK; Strong E; Holloway JK; Cohen PE; Schimenti J; Ward J; Hunter N. 2014. Antagonistic roles of ubiquitin ligase HEI10 and SUMO ligase RNF212 regulate meiotic recombination. Nat Genet 46(2):194-9. [PubMed: 24390283]  [MGI Ref ID J:205585]

Reynolds A; Qiao H; Yang Y; Chen JK; Jackson N; Biswas K; Holloway JK; Baudat F; de Massy B; Wang J; Hoog C; Cohen PE; Hunter N. 2013. RNF212 is a dosage-sensitive regulator of crossing-over during mammalian meiosis. Nat Genet 45(3):269-78. [PubMed: 23396135]  [MGI Ref ID J:196206]

Saferali A; Berlivet S; Schimenti J; Bartolomei MS; Taketo T; Naumova AK. 2010. Defective imprint resetting in carriers of Robertsonian translocation Rb (8.12). Mamm Genome 21(7-8):377-87. [PubMed: 20577743]  [MGI Ref ID J:163018]

Souquet B; Abby E; Herve R; Finsterbusch F; Tourpin S; Le Bouffant R; Duquenne C; Messiaen S; Martini E; Bernardino-Sgherri J; Toth A; Habert R; Livera G. 2013. MEIOB targets single-strand DNA and is necessary for meiotic recombination. PLoS Genet 9(9):e1003784. [PubMed: 24068956]  [MGI Ref ID J:203426]

Wojtasz L; Cloutier JM; Baumann M; Daniel K; Varga J; Fu J; Anastassiadis K; Stewart AF; Remenyi A; Turner JM; Toth A. 2012. Meiotic DNA double-strand breaks and chromosome asynapsis in mice are monitored by distinct HORMAD2-independent and -dependent mechanisms. Genes Dev 26(9):958-73. [PubMed: 22549958]  [MGI Ref ID J:183982]

Zenvirth D; Richler C; Bardhan A; Baudat F; Barzilai A; Wahrman J; Simchen G. 2003. Mammalian meiosis involves DNA double-strand breaks with 3' overhangs. Chromosoma 111(6):369-76. [PubMed: 12644952]  [MGI Ref ID J:83201]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as heterozygotes. Homozygotes are viable but infertile.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2450.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3185.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   +/+ from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.5)