Strain Name:

B6.129S6(FVB)-S1pr1tm2.1Rlp/J

Stock Number:

019141

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Availability:

Repository- Live

These S1pr1loxP/loxP mice possess loxP sites flanking exon 2. They may have applications in studies related to adaptive immune responses and vascular development.

Description

Strain Information

Former Names B6N.129S6(FVB)-S1pr1tm2.1Rlp/J    (Changed: 03-SEP-13 )
B6N.Cg-S1pr1tm2.1Rlp/J    (Changed: 30-JAN-13 )
Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Mating SystemHomozygote x Homozygote         (Female x Male)   30-AUG-13
Specieslaboratory mouse
GenerationN7+N1 (30-APR-13)
Generation Definitions
 
Donating Investigator Richard L Proia,   National Institutes of Health

Description
These S1pr1loxP/loxP mice possess loxP sites flanking exon 2 of the sphingosine-1-phosphate receptor 1 (S1pr1) gene. S1PR1 is a G protein-coupled receptor for lysophospholipid sphingosine-1-phosphate (S1P) and is highly expressed in endothelial cells. S1PR1 is essential for vascular maturation during embryonic development and is also involved in cell survival, migration, adhesion, and proliferation. This receptor plays a role in the regulation of innate and adaptive immune responses by controlling lymphocyte egress from the thymus, spleen, bone marrow, and lymph nodes. It has also been implicated in the regulation of vascular function. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in cre-expressing tissues.

For example, when crossed to B6.Cg-Tg(Tek-cre)1Ywa/J (Stock No. 008863) Mice expressing Cre recombinase in vascular endothelial cells during embryogenesis and adulthood, the resulting offspring die by E14.5 due to muscle defects including heart, vascular smooth muscle, and skeletal muscle defects.

Development
A targeting vector was designed to insert a single loxP site upstream of exon 2, and a loxP-flanked neomycin resistance (neo) cassette downstream of exon 2 of the sphingosine-1-phosphate receptor 1 (S1pr1) gene. The construct was electroporated into 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric males were bred with C57BL/6NCrl females. Offspring, heterozygous for this S1pr1loxP allele, were bred with B6.FVB-Tg(EIIa-cre)C5379Lmgd/J transgenic mice (Stock No. 003724) to delete the neo cassette. Resulting mice contained multiple gene rearrangments; intact floxed-exon 2, intact floxed-neo cassette, or excision of both exon 2 and the neo cassette. Progeny, containing only the floxed-exon 2, were crossed to remove the Cre-expressing transgene. The donating investigator reported that these mice were bred to C57BL/6NCrl mice for at least 7 generations (see SNP note below). Upon arrival, mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for at least one generation to establish the colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 3 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6J genetic background.

Control Information

  Control
   005304 C57BL/6NJ
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of S1pr1
021017   B6.129P2-S1pr1tm1.1Cys/J
013063   NOD.129S6(B6N)-S1pr1tm2Rlp/JbsJ
View Strains carrying other alleles of S1pr1     (2 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

S1pr1tm2.1Rlp/S1pr1+

        involves: 129S6/SvEvTac
  • no phenotypic analysis
  • *normal* no phenotypic analysis   (MGI Ref ID J:86450)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cancer Research
Genes Regulating Growth and Proliferation

Cardiovascular Research
Heart Abnormalities
Vascular Defects

Developmental Biology Research
Internal/Organ Defects
      heart: vasculature
      vasculature

Immunology, Inflammation and Autoimmunity Research
Lymphoid Tissue Defects
      Lymphocyte Homing

Research Tools
Cancer Research
      endothelial cell marker for neovascularization
Cardiovascular Research
      endothelial cell marker for neovascularization
Cre-lox System
      loxP-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol S1pr1tm2.1Rlp
Allele Name targeted mutation 2.1, Richard L Proia
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) S1pr1loxP;
Strain of Origin129S6/SvEvTac
Gene Symbol and Name S1pr1, sphingosine-1-phosphate receptor 1
Chromosome 3
Gene Common Name(s) AI849002; CD363; CHEDG1; D1S3362; ECGF1; EDG-1; EDG1; Edg1; S1P; S1P1; endothelial differentiation sphingolipid G-protein-coupled receptor 1; endothelial differentiation spingolipid G-protein-coupled receptor 1; expressed sequence AI849002;
Molecular Note A targeting vector was designed to insert a single loxP site upstream of exon 2, and a loxP-flanked neomycin resistance (neo) cassette downstream of exon 2. Cre-medaited recombination removed the neo cassette and left exon 2 floxed. [MGI Ref ID J:86450]

Genotyping

Genotyping Information

Genotyping Protocols

S1pr1tm2Rlp, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Allende ML; Yamashita T; Proia RL. 2003. G-protein-coupled receptor S1P1 acts within endothelial cells to regulate vascular maturation. Blood 102(10):3665-7. [PubMed: 12869509]  [MGI Ref ID J:86450]

Additional References

S1pr1tm2.1Rlp related

Arnon TI; Horton RM; Grigorova IL; Cyster JG. 2013. Visualization of splenic marginal zone B-cell shuttling and follicular B-cell egress. Nature 493(7434):684-8. [PubMed: 23263181]  [MGI Ref ID J:194402]

Park SW; Kim M; Kim JY; Brown KM; Haase VH; D'Agati VD; Lee HT. 2012. Proximal tubule sphingosine kinase-1 has a critical role in A1 adenosine receptor-mediated renal protection from ischemia. Kidney Int 82(8):878-91. [PubMed: 22695326]  [MGI Ref ID J:198171]

Priceman SJ; Shen S; Wang L; Deng J; Yue C; Kujawski M; Yu H. 2014. S1PR1 is crucial for accumulation of regulatory T cells in tumors via STAT3. Cell Rep 6(6):992-9. [PubMed: 24630990]  [MGI Ref ID J:211829]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX18

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice may be bred together.
Mating SystemHomozygote x Homozygote         (Female x Male)   30-AUG-13
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHomozygous for S1pr1tm2.1Rlp  
Price per Pair (US dollars $)Pair Genotype
$464.00Homozygous for S1pr1tm2.1Rlp x Homozygous for S1pr1tm2.1Rlp  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHomozygous for S1pr1tm2.1Rlp  
Price per Pair (US dollars $)Pair Genotype
$603.20Homozygous for S1pr1tm2.1Rlp x Homozygous for S1pr1tm2.1Rlp  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   005304 C57BL/6NJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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