|These PTIP-floxed mice possess loxP sites flanking exon 1 of the PAX interacting (with transcription-activation domain) protein 1 (Paxip1) gene and have applications in studies related to early embryonic development and transcriptional regulation.|
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Gregory R Dressler, University of Michigan
Paxip1, PAX interacting (with transcription-activation domain) protein 1, (PTIP) associates with histone H3K4 methyltransferases and is involved in DNA damage response, transcriptional regulation and progression through mitosis. These mice possess loxP sites on either side of exon 1 of the targeted gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 1 deleted in the cre-expressing tissues.
When bred to a strain with Cre recombinase expression in the renal inner medulla, this mutant mouse strain may be useful in studies of renal physiology, urine concentration and osmotic tolerance.
A targeting vector containing a FRT site flanked NEO cassette was utilized in the construction of this mutant. This selection cassette was inserted upstream of exon 1 of the targeted gene, and another loxP site was inserted downstream of exon 1. This construct was electroporated into 129 derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 X (C57BL/6 X DBA/2)F1 blastocysts. Resulting chimeric male animals were backcrossed to wildtype C57BL/6 mice. The mice were then crossed to transgenic mice (on the C57BL/6 genetic background) expressing FLP1 recombinase to remove the selection cassette. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
|Wild-type from the colony|
|Considerations for Choosing Controls|
View Related Disease (OMIM) TermsRelated Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.Alzheimer Disease; AD (PAXIP1)
View Mammalian Phenotype TermsMammalian Phenotype Terms provided by MGIassigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Paxip1tm2Gdr/Paxip1tm2Gdrinvolves: 129S1/Sv * 129X1/SvJ
- normal phenotype
- no abnormal phenotype detected
- mice have no distinguishable phenotype (MGI Ref ID J:128440)View Research Applications
|Allele Name||targeted mutation 1, Gregory R Dressler|
|Allele Type||Targeted (Floxed/Frt)|
|Common Name(s)||PTIPflox; ptipfl; ptiploxP;|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl<+>|
|Gene Symbol and Name||Paxip1, PAX interacting (with transcription-activation domain) protein 1|
|Gene Common Name(s)||CAGF29; D5Ertd149e; DNA segment, Chr 5, ERATO Doi 149, expressed; PACIP1; PAXIP1L; PTIP; TNRC2;|
|Molecular Note||A neo cassette flanked by FRT sites was inserted upstream of exon 1 and an additional loxP site was inserted downstream of exon 1. The neo cassette was subsequently removed by flp-mediated recombination. [MGI Ref ID J:128440] [MGI Ref ID J:148599]|
Patel SR; Kim D; Levitan I; Dressler GR. 2007. The BRCT-domain containing protein PTIP links PAX2 to a histone H3, lysine 4 methyltransferase complex. Dev Cell 13(4):580-92. [PubMed: 17925232] [MGI Ref ID J:128440]
Callen E; Faryabi RB; Luckey M; Hao B; Daniel JA; Yang W; Sun HW; Dressler G; Peng W; Chi H; Ge K; Krangel MS; Park JH; Nussenzweig A. 2012. The DNA damage- and transcription-associated protein paxip1 controls thymocyte development and emigration. Immunity 37(6):971-85. [PubMed: 23159437] [MGI Ref ID J:191076]
Cho YW; Hong S; Jin Q; Wang L; Lee JE; Gavrilova O; Ge K. 2009. Histone methylation regulator PTIP is required for PPARgamma and C/EBPalpha expression and adipogenesis. Cell Metab 10(1):27-39. [PubMed: 19583951] [MGI Ref ID J:151619]
Kim D; Wang M; Cai Q; Brooks H; Dressler GR. 2007. Pax transactivation-domain interacting protein is required for urine concentration and osmotolerance in collecting duct epithelia. J Am Soc Nephrol 18(5):1458-65. [PubMed: 17429055] [MGI Ref ID J:148599]
Lefevre GM; Patel SR; Kim D; Tessarollo L; Dressler GR. 2010. Altering a histone H3K4 methylation pathway in glomerular podocytes promotes a chronic disease phenotype. PLoS Genet 6(10):e1001142. [PubMed: 21060806] [MGI Ref ID J:167530]
Schwab KR; Patel SR; Dressler GR. 2011. Role of PTIP in Class Switch Recombination and Long-Range Chromatin Interactions at the Immunoglobulin Heavy Chain Locus. Mol Cell Biol 31(7):1503-11. [PubMed: 21282469] [MGI Ref ID J:170100]
Stein AB; Jones TA; Herron TJ; Patel SR; Day SM; Noujaim SF; Milstein ML; Klos M; Furspan PB; Jalife J; Dressler GR. 2011. Loss of H3K4 methylation destabilizes gene expression patterns and physiological functions in adult murine cardiomyocytes. J Clin Invest 121(7):2641-50. [PubMed: 21646717] [MGI Ref ID J:175659]
Wang P; Lin C; Smith ER; Guo H; Sanderson BW; Wu M; Gogol M; Alexander T; Seidel C; Wiedemann LM; Ge K; Krumlauf R; Shilatifard A. 2009. Global analysis of H3K4 methylation defines MLL family member targets and points to a role for MLL1-mediated H3K4 methylation in the regulation of transcriptional initiation by RNA polymerase II. Mol Cell Biol 29(22):6074-85. [PubMed: 19703992] [MGI Ref ID J:154938]
Breeding & Husbandry When maintaining a live colony, these mice can be bred as homozygotes. Diet Information LabDiet® 5K52/5K67
This strain is currently Under Development for Cryo.
|Wild-type from the colony|
|Considerations for Choosing Controls|
|Control Pricing Information for Genetically Engineered Mutant Strains.|
For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.