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| In this strain an IRES-lacZ cassette replaces exon 7 of the Tmc2 (transmembrane channel-like gene family 2) gene. These mice exhibit altered inner ear hair cell mechanotransduction and may be useful in studies of deafness and physiology of the inner ear. | |||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Donating Investigator Dr. Andrew J. Griffith, NIDCD/NIH Description
Tmc2 (transmembrane channel-like gene family 2) is expressed in the inner ear. These mice carry a targeted mutation in which IRES-lacZ replaces exon 7 (encoding the first transmembrane domain). Mice that are homozygous for the targeted mutation are viable and fertile. Mutant gene product (mRNA) lacking the lacZ sequence is detected by RT-PCR analysis of total RNA from inner ear tissue of P2 homozygotes. The Donating Investigator reports that beta-galactosidase activity is difficult to detect.
Double mutant mice that are homozygous for the Tmc1tm1.1Ajg (Stock No. 019146) and Tmc2tm1.1Ajg (Stock No. 019147) targeted mutation alleles are deaf, exhibit altered vestibulo-ocular reflex and lack mechanotransduction currents in the hair cells.Development
A targeting vector containing IRES-lacZ sequence and a floxed PGK-NEO cassette was used to disrupt exon 7. The construct was electroporated into 129 derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were crossed to C57BL/6-Tg(Zp3-cre)93Knw/J (Stock No. 003651) mice to remove the PKG-NEO cassette. The mice were then backcrossed to C57BL/6J for 15 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Phenotype
Phenotype Information
View Mammalian Phenotype Terms
Mammalian Phenotype Terms provided by MGI
assigned by genotype
Tmc2tm1.1Ajg/Tmc2tm1.1Ajg
B6.129-Tmc2tm1.1Ajg
- hearing/vestibular/ear phenotype
- *normal* hearing/vestibular/ear phenotype
- normal hearing (MGI Ref ID J:184419)
- abnormal cochlear hair cell physiology
- partially resistant to gentamicin induced toxicity with hair cell loss confined to the basal end of the cochlea (MGI Ref ID J:184419)
- behavior/neurological phenotype
- *normal* behavior/neurological phenotype
- normal motor behavior (MGI Ref ID J:184419)
- nervous system phenotype
- abnormal cochlear hair cell physiology
- partially resistant to gentamicin induced toxicity with hair cell loss confined to the basal end of the cochlea (MGI Ref ID J:184419)
This mouse can be used to support research in many areas including:
Neurobiology Research
Hearing Defects
Research Tools
lacZ Expression
Genetics Research
Tissue/Cell Markers
Sensorineural Research
Sensorineural Research
Hearing Defects
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tmc2tm1.1Ajg | ||
|---|---|---|---|
| Allele Name | targeted mutation 1.1, Andrew J Griffith | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | Tmc2delta; | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | Tmc2 is normally expressed in mouse vestibular and cochlear hair cells and GFP-tagged TMC proteins localize near stereocilia tips. Tmc2 expression is transient in early postnatal mouse cochlear hair cells but persists in vestibular hair cells. | ||
| Gene Symbol and Name | Tmc2, transmembrane channel-like gene family 2 | ||
| Chromosome | 2 | ||
| Gene Common Name(s) | C20orf145; dJ686C3.3; | ||
| Molecular Note | Exon 7 was replaced with an IRES-lacZ cassette and a floxed pgk-neo cassette via homologous recombination. Cre mediated recombination removed the neo cassette. At P28 beta-galactosidase activity is detected in hair cells of the cristae ampullaris and very faintly in in hair cells of the saccular and utricular maculae. [MGI Ref ID J:184419] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tmc2tm1.1Ajg, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Kawashima Y; Geleoc GS; Kurima K; Labay V; Lelli A; Asai Y; Makishima T; Wu DK; Della Santina CC; Holt JR; Griffith AJ. 2011. Mechanotransduction in mouse inner ear hair cells requires transmembrane channel-like genes. J Clin Invest 121(12):4796-809. [PubMed: 22105175] [MGI Ref ID J:184419]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as homozygotes.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
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