Strain Name:

B6.129P2-Acsl1tm1Rcol/J

Stock Number:

019360

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Availability:

Cryopreserved - Ready for recovery

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These mice possess loxP sites flanking exon 2 of the acyl-CoA synthetase long-chain family member 1 (Acsl1) gene and have applications in studies related to lipid metabolism and signaling, energy metabolism, and cardiac function.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Mating SystemHeterozygote x Heterozygote         (Female x Male)   10-JUL-13
Specieslaboratory mouse
 
Donating Investigator Rosalind Coleman,   University of North Carolina

Description
The isozyme encoded by the acyl-CoA synthetase long-chain family member 1 (Acsl1) gene catalyzes the synthesis of acyl-CoA from fatty acids that are between 10 and 22 carbons in size. These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues.

When bred to a strain with Cre recombinase expression in the liver (see Stock No. 003574 for example), the resulting mutant mouse strain exhibits a 50% decrease in total hepatic acyl-CoA synthetase enzyme activity and may be useful in studies of lipid metabolism and liver physiology.

When bred to a strain with Cre recombinase expression in adipose tissue (see Stock No. 005069 for example), the mutant mice exhibit a 80% decrease of total acyl-CoA synthetase activity in adipose tissue, marked deficiency of adipose tissue to oxidize fatty acids, and impaired thermogenesis.

When bred to a strain with tamoxifen-mediated Cre recombinase expression in heart (see Stock No. 005657 and Stock No. 005650, for examples), the mutant mice have a 90% decrease of total acyl-CoA synthetase activity in heart ventricles, and exhibit cardiac hypertrophy and diastolic dysfunction.

Development
A targeting vector containing FRT site flanked MC1-Neo selection cassette was utilized in the construction of this mutant. This selection cassette was inserted upstream of exon 2 of the targeted gene, and another loxP site was inserted downstream of exon 2. This construct was electroporated into 129P2/OlaHsd derived E14TG2a embryonic stem (ES) cells which were transiently transfected with a FLP1 (flpE) recombinase vector to remove the selection cassette. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to C57BL/6 for 7 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
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Acsl1tm1Rcol/Acsl1tm1Rcol Tg(Alb-cre)21Mgn/0

        involves: 129P2/OlaHsd * C57BL/6 * DBA   (conditional)
  • growth/size/body phenotype
  • *normal* growth/size/body phenotype
    • normal body weight and organ weights   (MGI Ref ID J:155714)
  • adipose tissue phenotype
  • *normal* adipose tissue phenotype
    • normal adiposity   (MGI Ref ID J:155714)
  • homeostasis/metabolism phenotype
  • *normal* homeostasis/metabolism phenotype
    • normal plasma levels of metabolites   (MGI Ref ID J:155714)
    • normal golucose tolerance tests and insulin tolerance tests   (MGI Ref ID J:155714)
    • abnormal lipid homeostasis
      • liver acyl-CoA levels are 35% lower than controls on a normal diet and 25% lower than controls on a high fat diet   (MGI Ref ID J:155714)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Research Tools
Cre-lox System
      loxP-flanked Sequences
Diabetes and Obesity Research
      loxP

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Acsl1tm1Rcol
Allele Name targeted mutation 1, Rosalind Coleman
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) Acsl1flox;
Strain of Origin129P2/OlaHsd
Gene Symbol and Name Acsl1, acyl-CoA synthetase long-chain family member 1
Chromosome 8
Gene Common Name(s) ACS; ACS1; Acas; Acas1; COAA; FACL1; FACL2; Facl2; LACS; LACS1; LACS2; fatty acid Coenzyme A ligase, long chain 2;
Molecular Note A loxP site and FRT flanked MC1-neo cassette were inserted 5' to exon 2 and an additional loxP site was placed immediately 3' of exon 2. The neomycin cassette was then excised by transient expression of Flpe recombinase. Cre expression in the liver of homozygous mice eliminates most mRNA for this gene. [MGI Ref ID J:155714]

Genotyping

Genotyping Information

Genotyping Protocols

Acsl1tm1Rcol, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Li LO; Ellis JM; Paich HA; Wang S; Gong N; Altshuller G; Thresher RJ; Koves TR; Watkins SM; Muoio DM; Cline GW; Shulman GI; Coleman RA. 2009. Liver-specific loss of long chain acyl-CoA synthetase-1 decreases triacylglycerol synthesis and beta-oxidation and alters phospholipid fatty acid composition. J Biol Chem 284(41):27816-26. [PubMed: 19648649]  [MGI Ref ID J:155714]

Additional References

Acsl1tm1Rcol related

Ellis JM; Li LO; Wu PC; Koves TR; Ilkayeva O; Stevens RD; Watkins SM; Muoio DM; Coleman RA. 2010. Adipose acyl-CoA synthetase-1 directs fatty acids toward beta-oxidation and is required for cold thermogenesis. Cell Metab 12(1):53-64. [PubMed: 20620995]  [MGI Ref ID J:162996]

Kanter JE; Kramer F; Barnhart S; Averill MM; Vivekanandan-Giri A; Vickery T; Li LO; Becker L; Yuan W; Chait A; Braun KR; Potter-Perigo S; Sanda S; Wight TN; Pennathur S; Serhan CN; Heinecke JW; Coleman RA; Bornfeldt KE. 2012. Diabetes promotes an inflammatory macrophage phenotype and atherosclerosis through acyl-CoA synthetase 1. Proc Natl Acad Sci U S A 109(12):E715-24. [PubMed: 22308341]  [MGI Ref ID J:182631]

Kanter JE; Tang C; Oram JF; Bornfeldt KE. 2012. Acyl-CoA synthetase 1 is required for oleate and linoleate mediated inhibition of cholesterol efflux through ATP-binding cassette transporter A1 in macrophages. Biochim Biophys Acta 1821(3):358-64. [PubMed: 22020260]  [MGI Ref ID J:182368]

Li S; Sun Y; Liang CP; Thorp EB; Han S; Jehle AW; Saraswathi V; Pridgen B; Kanter JE; Li R; Welch CL; Hasty AH; Bornfeldt KE; Breslow JL; Tabas I; Tall AR. 2009. Defective phagocytosis of apoptotic cells by macrophages in atherosclerotic lesions of ob/ob mice and reversal by a fish oil diet. Circ Res 105(11):1072-82. [PubMed: 19834009]  [MGI Ref ID J:170144]

Paul DS; Grevengoed TJ; Pascual F; Ellis JM; Willis MS; Coleman RA. 2014. Deficiency of cardiac Acyl-CoA synthetase-1 induces diastolic dysfunction, but pathologic hypertrophy is reversed by rapamycin. Biochim Biophys Acta 1841(6):880-7. [PubMed: 24631848]  [MGI Ref ID J:211553]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as homozygotes.
Mating SystemHeterozygote x Heterozygote         (Female x Male)   10-JUL-13

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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