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| Zfp423GFP BAC transgenic mice express GFP in the preadipocytes. | |||||||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Noncarrier x Hemizygote (Female x Male) 16-APR-13 Mating System Hemizygote x Noncarrier (Female x Male) 16-APR-13 Species laboratory mouse Donating Investigator Rana Gupta, UT Southwestern Medical Center Description
Zfp423GFP BAC transgenic mice are viable and fertile, with enhanced green fluorescent protein (EGFP) expression under control of the zinc finger protein 423 (Zfp423) promoter/enhancer regions within the BAC transgene. ZFP423 is involved in regulating neuronal and adipocyte differentiation. These mice express GFP in preadipocytes in the subcutaneous and visceral stromal-vasculature within white and brown fat.Development
The RP23-102G4 bacterial artificial chromosome (BAC) containing exons 1-2 of the zinc finger protein 423 (Zfp423) locus and 150kb of upstream regulatory sequence was obtained. This BAC was modified by targeting an enhanced green fluorescent protein (EGFP) and a polyadenylation sequence into the first exon of Zfp423. No other genes are encoded by this BAC. This modified ~200 kb BAC transgene was microinjected into the pronuclei of fertilized FVB oocytes. Transgenic mice from founder line 7 were identified and bred to C57BL/6J mice. The donating investigator reports that the transgene inserted in the X chromosome. These Zfp423GFP mice were subsequently maintained by breeding transgenic mice with wildtype male C57BL/6J or wildtype male siblings. Upon arrival at The Jackson Laboratory Repository, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of GFP
View Strains carrying other alleles of GFP (307 strains)
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
GFP relatedResearch Tools
Cardiovascular Research
endothelial cell marker for neovascularization
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Zfp423-EGFP)7Brsp | ||
|---|---|---|---|
| Allele Name | transgene insertion 7, Bruce M Spiegelman | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | Zfp423GFP; | ||
| Mutation Made By | Rana Gupta, UT Southwestern Medical Center | ||
| Site of Expression | These mice express GFP in preadipocytes. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Zfp423, zinc finger protein 423, mouse, laboratory | ||
| Molecular Note | Coding sequence of EGFP followed by a polyadenylationsignal was inserted into the initiation codon of Zfp423 located in exon 1 from bacterial artificial chromosome RP23-102G4. The modified BAC does not contain any functional domains of Zfp423 or contain full-length coding sequence of any other annotated genes. Five founder lines were produced, two of which were greatly enriched for Zfp423 mRNA in cells positive for EGFP. Data for one of these two lines was published. [MGI Ref ID J:182285] | ||
| Gene Symbol and Name | Tg(Zfp423-EGFP)7Brsp, transgene insertion 7, Bruce M Spiegelman | ||
| Chromosome | UN | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Zfp423-EGFP)7Brsp, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Gupta RK; Mepani RJ; Kleiner S; Lo JC; Khandekar MJ; Cohen P; Frontini A; Bhowmick DC; Ye L; Cinti S; Spiegelman BM. 2012. Zfp423 expression identifies committed preadipocytes and localizes to adipose endothelial and perivascular cells. Cell Metab 15(2):230-9. [PubMed: 22326224] [MGI Ref ID J:182285]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous females may be bred to wildtype (non-carrier) males from the colony. The donating investigator reports that the transgene inserted in the X chromosome. Mating System Noncarrier x Hemizygote (Female x Male) 16-APR-13 Hemizygote x Noncarrier (Female x Male) 16-APR-13
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
This strain is currently Under Development - Now Accepting Orders.
Estimated Available for Distribution Date:
12-AUG-13
Please note: You may now place orders for this strain although it is not yet ready for distribution. Estimated available for distribution dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for distribution depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain.
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Hemizygous for Tg(Zfp423-EGFP)7Brsp
Price per Pair (US dollars $) Pair Genotype $296.00 Hemizygous for Tg(Zfp423-EGFP)7Brsp x Noncarrier $296.00 Noncarrier x Hemizygous for Tg(Zfp423-EGFP)7Brsp Standard Supply
Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.
| Pricing for International shipping destinations |
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Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Hemizygous for Tg(Zfp423-EGFP)7Brsp
Price per Pair (US dollars $) Pair Genotype $384.80 Hemizygous for Tg(Zfp423-EGFP)7Brsp x Noncarrier $384.80 Noncarrier x Hemizygous for Tg(Zfp423-EGFP)7Brsp Standard Supply
Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.
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Standard Supply
Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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