Strain Name:

FVB.129-Ets2tm3Rgo/J

Stock Number:

020455

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Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
The Ets2flox allele has loxP sites flanking the C-terminal portion of the E26 avian leukemia oncogene 2 DNA-binding domain (floxed exons 9-10). These Ets2flox mice may be useful in studying both tumor repressive and tumor supportive roles in different types of cancer, epithelial tumor microenvironments, and endothelial cell function/survival during embryonic angiogenesis.

Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Mating SystemHomozygote x Homozygote         (Female x Male)   19-JUL-13
Specieslaboratory mouse
GenerationN9pN1+F1 (14-MAY-13)
Generation Definitions
 
Donating Investigator Robert G Oshima,   Sanford-Burnham Medical Research Institute

Description
Homozygous Ets2flox mice are viable and fertile. The Ets2flox allele has loxP sites flanking exons 9-10 of the E26 avian leukemia oncogene 2 (Ets2) gene. When bred to mice that express Cre recombinase, the resulting offspring will have the sequences encoding the C-terminal portion of the Ets2 DNA-binding domain deleted in the cre-expressing tissues. This is designed to result in an inactive Ets2 protein lacking a functional DNA-binding domain (the deleted allele is called Ets2db2).

When Ets2flox mice are bred to mice with Cre recombinase expression in embryonic/germ cells (MORE-Cre; Stock No. 003755), the resulting homozygous mice with pan deletion of the Ets2 DNA binding domain exhibit embryonic lethality similar to Ets2 knockout mice. Furthermore, such mice may be used to study mammary epithelial tumor microenvironment when bred to also harbor transgenes expressing either Polyoma virus middle T antigen (see MMTV-PyVT mice [Stock No. 002374]) or activated Neu/ErbB2 (see MMTV-Neu/ErbB2 mice [Stock No. 005038]).

In addition, when Ets2flox mice are bred to a strain expressing Cre recombinase in intestinal epithelial tissues (Villin-Cre; Stock No. 004586), the resulting mice are useful in studying intestinal stem cells, colon crypt development, and chemical carcinogen-induced colon tumorigenesis.

Development
A targeting vector was designed to insert an frt-flanked PGK-neo cassette and a loxP site upstream of exon 9, and a second loxP site downstream of exon 10 of the E26 avian leukemia oncogene 2 (Ets2) gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with FVB/NJ mice to establish the Ets2flox-neo colony. To remove the frt-flanked PGK-neo cassette, embryos from Ets2flox-neo mice were isolated and then microinjected with a pCAGGS-Flpe plasmid. The resulting floxed exons 9-10 allele (a single frt site and first loxP site remaining upstream of exon 9, and a second loxP site downstream of exon 10) is called Ets2flox. These Ets2flox mice were subsequently backcrossed to FVB/NJ mice for at least nine generations prior to sending to The Jackson Laboratory Repository. Upon arrival, mice were bred to FVB/NJ inbred mice (Stock No. 001800) for at least one generation to establish The Jackson Laboratory Repository colony.

Control Information

  Control
   001800 FVB/NJ
 
  Considerations for Choosing Controls

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Ets2tm3Rgo/Ets2tm3Rgo

        involves: 129S1/Sv * 129X1/SvJ
  • normal phenotype
  • no abnormal phenotype detected
    • mice have normal development and lifespan   (MGI Ref ID J:128952)

Ets2tm3Rgo/Ets2tm3Rgo

        involves: 129S1/Sv * 129X1/SvJ   (conditional)
  • embryogenesis phenotype
  • abnormal trophoblast layer morphology
    • when transfected with an adenovirus Cre, trophoblast stem cells exhibit reduced growth and self-renewal   (MGI Ref ID J:128952)
    • however, trophoblast cells transfected with an adenovirus Cre differentiate normally   (MGI Ref ID J:128952)

The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.

Ets2tm3Rgo/Ets2tm3Rgo Meox2tm1(cre)Sor/Meox2+

        involves: 129S1/Sv * 129S4/SvJaeSor * 129X1/SvJ   (conditional)
  • mortality/aging
  • complete embryonic lethality between somite formation and embryo turning
    • mice die around E8   (MGI Ref ID J:128952)
  • embryogenesis phenotype
  • abnormal visceral yolk sac morphology
    • at E7.5, yolk sacs are cone-shaped   (MGI Ref ID J:128952)
  • decreased embryo size
  • growth/size/body phenotype
  • decreased embryo size
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cancer Research
Genes Regulating Growth and Proliferation
Growth Factors/Receptors/Cytokines
Oncogenes
Other
Tumor Resistance
Tumor Suppressor Genes

Cell Biology Research
Signal Transduction
Transcriptional Regulation

Immunology, Inflammation and Autoimmunity Research
Intracellular Signaling Molecules

Research Tools
Cancer Research
      anti-tumor activity
Cre-lox System
      loxP-flanked Sequences
Genetics Research
      Mutagenesis and Transgenesis: Cre-lox System
      Mutagenesis and Transgenesis: transcriptional activation

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Ets2tm3Rgo
Allele Name targeted mutation 3, Robert G Oshima
Allele Type Targeted (Floxed/Frt)
Common Name(s) Ets2flox; Ets2tm4Rgo;
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name Ets2, E26 avian leukemia oncogene 2, 3' domain
Chromosome 16
Gene Common Name(s) AU022856; ETS2IT1; Ets-2; expressed sequence AU022856;
Molecular Note An frt-flanked neo cassette with a 3' loxP site was inserted into intron 8 and an additional loxP site was inserted into the 3' UTR. The neo cassette was removed by flp-mediated recombination using a transiently expressing vector. [MGI Ref ID J:128952]

Genotyping

Genotyping Information

Genotyping Protocols

Ets2tm3Rgo, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Munera J; Cecena G; Jedlicka P; Wankell M; Oshima RG. 2011. Ets2 regulates colonic stem cells and sensitivity to tumorigenesis. Stem Cells 29(3):430-9. [PubMed: 21425406]  [MGI Ref ID J:171112]

Tynan JA; Wen F; Muller WJ; Oshima RG. 2005. Ets2-dependent microenvironmental support of mouse mammary tumors. Oncogene 24(46):6870-6. [PubMed: 16007139]  [MGI Ref ID J:102368]

Wen F; Tynan JA; Cecena G; Williams R; Munera J; Mavrothalassitis G; Oshima RG. 2007. Ets2 is required for trophoblast stem cell self-renewal. Dev Biol 312(1):284-99. [PubMed: 17977525]  [MGI Ref ID J:128952]

Additional References

Ets2tm3Rgo related

Morioka Y; Isotani A; Oshima RG; Okabe M; Ikawa M. 2009. Placenta-specific gene activation and inactivation using integrase-defective lentiviral vectors with the Cre/LoxP system. Genesis 47(12):793-8. [PubMed: 19830817]  [MGI Ref ID J:156029]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX18

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice may be bred together.
Mating SystemHomozygote x Homozygote         (Female x Male)   19-JUL-13
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHomozygous for Ets2tm3Rgo  
Price per Pair (US dollars $)Pair Genotype
$464.00Homozygous for Ets2tm3Rgo x Homozygous for Ets2tm3Rgo  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHomozygous for Ets2tm3Rgo  
Price per Pair (US dollars $)Pair Genotype
$603.20Homozygous for Ets2tm3Rgo x Homozygous for Ets2tm3Rgo  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Control Information

  Control
   001800 FVB/NJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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