Description

Strain Information

Former Names STOCK Rictortm1Klg/SjmJ    (Changed: 14-JAN-13 ) Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Heterozygote x Heterozygote         (Female x Male)   04-MAR-13 Species laboratory mouse Generation ?+pN1 (17-MAY-13) Generation Definitions   Donating Investigator Sean J Morrison,   UT Southwestern

Description
These Rictor^F/F mutant mice possess loxP sites flanking exon 11 of the RPTOR independent companion of MTOR, complex 2 (Rictor) gene. Mice that are homozygous for this allele are viable and fertile. Rictor is part of a complex mTORC2, which regulates cell growth and survival. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 11 deleted in the cre-expressing tissues.

For example, when bred to C.Cg-Tg(Mx1-cre)1Cgn/J mice (Stock No. 005673) expressing cre in interferon-responsive cells, Rictor deletion in thymocytes in both bone marrow and thymus, after induction with polyinosinic-polycytidylic acid (pIpC), results in reduced proliferation of immature thymocytes.

Development
A targeting vector was designed to insert a loxP site followed by a frt-flanked neomycin resistance (neo) cassette upstream of exon 11, and a second loxP site downstream of exon 11 of the RPTOR independent companion of MTOR, complex 2 (Rictor) gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl⁺-dervied R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric males were bred to C57BL/6J females. Offspring were bred with Flp transgenic mice to delete the neo cassette, and progeny were crossed to remove the Flp-expressing transgene. These Rictor^F/F mice were bred to C57BL/6J mice for at least 6 generations, and were then backcrossed to C57BL/Ka CD45.2:Thy1.1 (Ptprc^b Thy1^a) mice for 3 generations. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation, and the CD45.2:Thy1.1 alleles were bred out of the colony.

Control Information

	Control
	Wild-type from the colony
	000664 C57BL/6J	(approximate)
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Rictor^tm1.1Klg/Rictor^tm1.1Klg Tg(Mx1-cre)1Cgn/0

        B6.Cg-Rictor^tm1.1Klg Tg(Mx1-cre)1Cgn   (conditional)

• immune system phenotype
• *normal* immune system phenotype
  • mice exhibit the normal frequency of mature T cells   (MGI Ref ID J:189736)
• • abnormal double-negative T cell morphology
    • the percentage of DN3 is increased while the percentage of DN1 and DN4 cells is reduced   (MGI Ref ID J:189736)
  • • decreased DN1 thymic pro-T cell number   (MGI Ref ID J:189736)
    • decreased DN2 thymocyte number   (MGI Ref ID J:189736)
    • decreased DN3 thymocyte number   (MGI Ref ID J:189736)
    • decreased DN4 thymocyte number   (MGI Ref ID J:189736)
    • increased double-negative T cell number   (MGI Ref ID J:189736)
  • decreased T cell proliferation
    • cell-intrinsic reduction of DN3 and DN4 thymocyte proliferation without an increase in apoptosis   (MGI Ref ID J:189736)
  • decreased double-positive T cell number   (MGI Ref ID J:189736)
  • decreased single-positive T cell number
    • of mature T cells in the thymus   (MGI Ref ID J:189736)
  • decreased thymus weight   (MGI Ref ID J:189736)
  • thymus hypoplasia   (MGI Ref ID J:189736)
• hematopoietic system phenotype
• *normal* hematopoietic system phenotype
  • mice exhibit normal adult hematopoiesis   (MGI Ref ID J:189736)
• • abnormal double-negative T cell morphology
    • the percentage of DN3 is increased while the percentage of DN1 and DN4 cells is reduced   (MGI Ref ID J:189736)
  • • decreased DN1 thymic pro-T cell number   (MGI Ref ID J:189736)
    • decreased DN2 thymocyte number   (MGI Ref ID J:189736)
    • decreased DN3 thymocyte number   (MGI Ref ID J:189736)
    • decreased DN4 thymocyte number   (MGI Ref ID J:189736)
    • increased double-negative T cell number   (MGI Ref ID J:189736)
  • decreased T cell proliferation
    • cell-intrinsic reduction of DN3 and DN4 thymocyte proliferation without an increase in apoptosis   (MGI Ref ID J:189736)
  • decreased double-positive T cell number   (MGI Ref ID J:189736)
  • decreased single-positive T cell number
    • of mature T cells in the thymus   (MGI Ref ID J:189736)
  • decreased thymus weight   (MGI Ref ID J:189736)
  • thymus hypoplasia   (MGI Ref ID J:189736)
• cellular phenotype
• abnormal double-negative T cell morphology
  • the percentage of DN3 is increased while the percentage of DN1 and DN4 cells is reduced   (MGI Ref ID J:189736)
• • decreased DN1 thymic pro-T cell number   (MGI Ref ID J:189736)
  • decreased DN2 thymocyte number   (MGI Ref ID J:189736)
  • decreased DN3 thymocyte number   (MGI Ref ID J:189736)
  • decreased DN4 thymocyte number   (MGI Ref ID J:189736)
  • increased double-negative T cell number   (MGI Ref ID J:189736)
• decreased T cell proliferation
  • cell-intrinsic reduction of DN3 and DN4 thymocyte proliferation without an increase in apoptosis   (MGI Ref ID J:189736)
• decreased double-positive T cell number   (MGI Ref ID J:189736)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cell Biology Research
Genes Regulating Growth and Proliferation

Research Tools
Cre-lox System
      loxP-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Rictortm1.1Klg
Allele Name		targeted mutation 1.1, Kun-Liang Guan
Allele Type		Targeted (Floxed/Frt)
Common Name(s)		RictorF;
Strain of Origin		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name		Rictor, RPTOR independent companion of MTOR, complex 2
Chromosome		15
Gene Common Name(s)		4921505C17Rik; 6030405M08Rik; AW492497; D530039E11Rik; Fyb; PIA; RIKEN cDNA 4921505C17 gene; RIKEN cDNA 6030405M08 gene; RIKEN cDNA D530039E11 gene; expressed sequence AW492497; mAVO3;
Molecular Note		A targeting vector was designed to insert a loxP site followed by a frt-flanked neomycin resistance (neo) cassette upstream of exon 11, and a second loxP site downstream of exon 11. Flp-mediated recombination removed the neo cassette and left exon 11 floxed. [MGI Ref ID J:189736] [MGI Ref ID J:191342]

Genotyping

Genotyping Information

Genotyping Protocols

Rictor^tm1Klg, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Magee JA; Ikenoue T; Nakada D; Lee JY; Guan KL; Morrison SJ. 2012. Temporal changes in PTEN and mTORC2 regulation of hematopoietic stem cell self-renewal and leukemia suppression. Cell Stem Cell 11(3):415-28. [PubMed: 22958933]  [MGI Ref ID J:191342]

Tang F; Wu Q; Ikenoue T; Guan KL; Liu Y; Zheng P. 2012. A critical role for Rictor in T lymphopoiesis. J Immunol 189(4):1850-7. [PubMed: 22815285]  [MGI Ref ID J:189736]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX18

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, homozygous mice may be bred together.
Mating System	Heterozygote x Heterozygote         (Female x Male)   04-MAR-13

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

 

This strain is currently Under Development - Now Accepting Orders.
Estimated Available for Distribution Date: 24-JUN-13

Please note: You may now place orders for this strain although it is not yet ready for distribution. Estimated available for distribution dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for distribution depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain.

Control Information

	Control
	Wild-type from the colony
	000664 C57BL/6J	(approximate)
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering Information
JAX^® Mice
Surgical and Preconditioning Services
JAX^® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
- Use of MICE by companies or for-profit entities requires a license.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.