Strain Name:

STOCK Rictortm1.1Klg/SjmJ

Stock Number:

020649

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Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
This RictorF/F conditional deletion strain may be useful for studying the role of mTORC2 complex in growth and development.

Description

Strain Information

Former Names STOCK Rictortm1Klg/SjmJ    (Changed: 14-JAN-13 )
Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Mating SystemHomozygote x Homozygote         (Female x Male)   03-MAR-14
Specieslaboratory mouse
Generation?+pN2F3 (10-APR-14)
Generation Definitions
 
Donating Investigator Sean J Morrison,   UT Southwestern

Description
These RictorF/F mutant mice possess loxP sites flanking exon 11 of the RPTOR independent companion of MTOR, complex 2 (Rictor) gene. Mice that are homozygous for this allele are viable and fertile. Rictor is part of a complex mTORC2, which regulates cell growth and survival. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 11 deleted in the cre-expressing tissues.

For example, when bred to C.Cg-Tg(Mx1-cre)1Cgn/J mice (Stock No. 005673) expressing cre in interferon-responsive cells, Rictor deletion in thymocytes in both bone marrow and thymus, after induction with polyinosinic-polycytidylic acid (pIpC), results in reduced proliferation of immature thymocytes.

Development
A targeting vector was designed to insert a loxP site followed by a frt-flanked neomycin resistance (neo) cassette upstream of exon 11, and a second loxP site downstream of exon 11 of the RPTOR independent companion of MTOR, complex 2 (Rictor) gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-dervied R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric males were bred to C57BL/6J females. Offspring were bred with Flp transgenic mice to delete the neo cassette, and progeny were crossed to remove the Flp-expressing transgene. These RictorF/F mice were bred to C57BL/6J mice for at least six generations, and were then backcrossed to C57BL/Ka CD45.2:Thy1.1 (Ptprcb Thy1a) mice for three generations before sending to The Jackson Laboratory Repository. Upon arrival, sperm was cryopreserved. To generate the living colony, an aliquot of the frozen sperm was used to fertilize oocytes from C57BL/6J female mice (Stock No. 000664). The living colony will be maintained as homozygous for the RictorF/F allele, and homozygous for the Ptprcb (CD45.2) and Thy1b (Thy1.2) surface marker alleles normally present for C57BL/6 mice.

Control Information

  Control
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
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Rictortm1.1Klg/Rictortm1.1Klg Tg(Mx1-cre)1Cgn/0

        B6.Cg-Rictortm1.1Klg Tg(Mx1-cre)1Cgn   (conditional)
  • immune system phenotype
  • *normal* immune system phenotype
    • mice exhibit the normal frequency of mature T cells   (MGI Ref ID J:189736)
    • abnormal double-negative T cell morphology
      • the percentage of DN3 is increased while the percentage of DN1 and DN4 cells is reduced   (MGI Ref ID J:189736)
      • decreased DN1 thymic pro-T cell number   (MGI Ref ID J:189736)
      • decreased DN2 thymocyte number   (MGI Ref ID J:189736)
      • decreased DN3 thymocyte number   (MGI Ref ID J:189736)
      • decreased DN4 thymocyte number   (MGI Ref ID J:189736)
      • increased double-negative T cell number   (MGI Ref ID J:189736)
    • decreased T cell proliferation
      • cell-intrinsic reduction of DN3 and DN4 thymocyte proliferation without an increase in apoptosis   (MGI Ref ID J:189736)
    • decreased double-positive T cell number   (MGI Ref ID J:189736)
    • decreased single-positive T cell number
      • of mature T cells in the thymus   (MGI Ref ID J:189736)
    • decreased thymus weight   (MGI Ref ID J:189736)
    • thymus hypoplasia   (MGI Ref ID J:189736)
  • hematopoietic system phenotype
  • *normal* hematopoietic system phenotype
    • mice exhibit normal adult hematopoiesis   (MGI Ref ID J:189736)
    • abnormal double-negative T cell morphology
      • the percentage of DN3 is increased while the percentage of DN1 and DN4 cells is reduced   (MGI Ref ID J:189736)
      • decreased DN1 thymic pro-T cell number   (MGI Ref ID J:189736)
      • decreased DN2 thymocyte number   (MGI Ref ID J:189736)
      • decreased DN3 thymocyte number   (MGI Ref ID J:189736)
      • decreased DN4 thymocyte number   (MGI Ref ID J:189736)
      • increased double-negative T cell number   (MGI Ref ID J:189736)
    • decreased T cell proliferation
      • cell-intrinsic reduction of DN3 and DN4 thymocyte proliferation without an increase in apoptosis   (MGI Ref ID J:189736)
    • decreased double-positive T cell number   (MGI Ref ID J:189736)
    • decreased single-positive T cell number
      • of mature T cells in the thymus   (MGI Ref ID J:189736)
    • decreased thymus weight   (MGI Ref ID J:189736)
    • thymus hypoplasia   (MGI Ref ID J:189736)
  • endocrine/exocrine gland phenotype
  • decreased DN1 thymic pro-T cell number   (MGI Ref ID J:189736)
  • decreased DN2 thymocyte number   (MGI Ref ID J:189736)
  • decreased DN3 thymocyte number   (MGI Ref ID J:189736)
  • decreased DN4 thymocyte number   (MGI Ref ID J:189736)
  • decreased thymus weight   (MGI Ref ID J:189736)
  • thymus hypoplasia   (MGI Ref ID J:189736)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cell Biology Research
Genes Regulating Growth and Proliferation

Research Tools
Cre-lox System
      loxP-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Rictortm1.1Klg
Allele Name targeted mutation 1.1, Kun-Liang Guan
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) RictorF;
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name Rictor, RPTOR independent companion of MTOR, complex 2
Chromosome 15
Gene Common Name(s) 4921505C17Rik; 6030405M08Rik; AVO3; AW492497; D530039E11Rik; Fyb; PIA; RIKEN cDNA 4921505C17 gene; RIKEN cDNA 6030405M08 gene; RIKEN cDNA D530039E11 gene; expressed sequence AW492497; hAVO3;
Molecular Note A targeting vector was designed to insert a loxP site followed by a frt-flanked neomycin resistance (neo) cassette upstream of exon 11, and a second loxP site downstream of exon 11. Flp-mediated recombination removed the neo cassette and left exon 11 floxed. [MGI Ref ID J:189736] [MGI Ref ID J:191342]

Genotyping

Genotyping Information

Genotyping Protocols

Rictortm1Klg, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Magee JA; Ikenoue T; Nakada D; Lee JY; Guan KL; Morrison SJ. 2012. Temporal changes in PTEN and mTORC2 regulation of hematopoietic stem cell self-renewal and leukemia suppression. Cell Stem Cell 11(3):415-28. [PubMed: 22958933]  [MGI Ref ID J:191342]

Tang F; Wu Q; Ikenoue T; Guan KL; Liu Y; Zheng P. 2012. A critical role for Rictor in T lymphopoiesis. J Immunol 189(4):1850-7. [PubMed: 22815285]  [MGI Ref ID J:189736]

Additional References

Rictortm1.1Klg related

Signer RA; Magee JA; Salic A; Morrison SJ. 2014. Haematopoietic stem cells require a highly regulated protein synthesis rate. Nature 509(7498):49-54. [PubMed: 24670665]  [MGI Ref ID J:210464]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX18

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice may be bred together.
Mating SystemHomozygote x Homozygote         (Female x Male)   03-MAR-14
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHomozygous for Rictortm1.1Klg  
Price per Pair (US dollars $)Pair Genotype
$464.00Homozygous for Rictortm1.1Klg x Homozygous for Rictortm1.1Klg  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHomozygous for Rictortm1.1Klg  
Price per Pair (US dollars $)Pair Genotype
$603.20Homozygous for Rictortm1.1Klg x Homozygous for Rictortm1.1Klg  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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