|These transgenic mice express mouse Mif (macrophage migration inhibitory factor)under the control of a tetracycline operator (tetO). When combined with a rat Clara-cell secretory protein (CCSP) promoter/rtTA allele, the lung-directed overexpression of MIF is sufficient to promote Lewis lung carcinoma (LLC)-initiated tumor growth after two weeks of doxycycline treatment.|
Former Names B6(SJL)-Tg(tetO-Mif)279Aren/J (Changed: 07-MAR-13 ) Type Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation +pN1
Donating Investigator Dr. Douglas Arenberg, University of Michigan
MIF (macrophage migration inhibitory factor, murine) is a cytokine that is expressed during tissue repair and tumor growth and has been shown to inhibit apoptosis. It is expressed in the lung in response to induced bleomycin and naphthalene damage of the epithelium.
These transgenic mice express mouse Mif under the control of a tetracycline operator (tetO). When bred with another mouse expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), MIF expression can be regulated with the tetracycline analog doxycycline (dox) in the resulting double mutant offspring.
When combined with a rat Clara-cell secretory protein (CCSP) promoter/rtTA allele (see Stock No. 006232), the lung-directed overexpression of MIF is sufficient to promote Lewis lung carcinoma (LLC)-initiated tumor growth after two weeks of doxycycline treatment.
The coat color of hemizygous and wildtype mice may be either black or agouti.
Mouse Mif cDNA isolated from C57BL/6-derived Lewis lung carcinoma (LLC) cells was inserted into an expression cassette consisting of seven tetO repeats, a cytomegalovirus minimal promoter, and a bovine growth hormone polyadenylation sequence. The transgenic vector was introduced to B6SJL hybrid embryos. The donating investigator reported the mice were backcrossed to C57BL/6 mice for at least seven generations (see SNP results below) prior to sending males to The Jackson Laboratory Repository in 2013. Upon arrival, males were used to cryopreserve sperm. To establish the living mouse colony, an aliquot of the frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
In 2013, a 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the first generation rederived living colony at The Jackson Laboratory Repository. While 26 of 27 markers were fixed for C57BL/6 allele-type, one marker on chromosome 1 was not C57BL/6 allele-type in some mice tested (e.g., still segregating for SJL allele-type). In addition, 4 of 5 markers that determine C57BL/6J from C57BL/6N were also found to be segregating on chromosomes 8, 13, 15 and 19 (note that these markers are the same for C57BL/6N and SJL). These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N or a mixed C57BL/6N;C57BL/6J genetic background.
|Considerations for Choosing Controls|
View Strains carrying other alleles of Mif (2 strains)Strains carrying other alleles of tetO
008079 129S-Ppargtm2Yba/J 016178 B6(Cg)-Tg(tetO-Cry2)3Jt/J 016176 B6(Cg)-Tg(tetO-Per2)2Jt/J 009602 B6.129S4(Cg)-Kcnn2tm2Jpad/J 009603 B6.129S4-Kcnn3tm1Jpad/J 023910 B6.Cg-Col1a1tm1(tetO-Lin28a)Gqda/J 023911 B6.Cg-Col1a1tm2(tetO-LIN28B)Gqda/J 023912 B6.Cg-Col1a1tm3(tetO-Mirlet7g/Mir21)Gqda/J 017983 B6.Cg-Col1a1tm9(tetO-Dnmt3b_i1)Jae Gt(ROSA)26Sortm1(rtTA*M2)Jae/J 014588 B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm6(tetO-MSI2)Jae/J 023749 B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae Tg(tetO-Pou5f1,-Sox2,-Klf4,-Myc)1Srn/J 014648 B6.Cg-Gt(ROSA)26Sortm37(H1/tetO-RNAi:Taz)Arte/ZkhuJ 006361 B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J 016998 B6.Cg-Tg(TetO-Axin1,EGFP)TA6Cos/J 003762 B6.Cg-Tg(tetFosb)4468Nes/J 007051 B6.Cg-Tg(tetO-APPSwInd)102Dbo/Mmjax 007052 B6.Cg-Tg(tetO-APPSwInd)107Dbo/Mmjax 007049 B6.Cg-Tg(tetO-APPSwInd)885Dbo/Mmjax 007618 B6.Cg-Tg(tetO-Arntl)1Jt/J 017555 B6.Cg-Tg(tetO-CALY)5Cber/J 024114 B6.Cg-Tg(tetO-CHRM4*)2Blr/J 008277 B6.Cg-Tg(tetO-Clockm1Jt)CL57Jt/J 008468 B6.Cg-Tg(tetO-DTA)1Gfi/J 017791 B6.Cg-Tg(tetO-Hamp)2181Nca/J 009344 B6.Cg-Tg(tetO-Ifng)184Pop/J 009136 B6.Cg-Tg(tetO-Kcnj2,lacZ)1Gogo/J 013583 B6.Cg-Tg(tetO-LRRK2)C7874Cai/J 017331 B6.Cg-Tg(tetO-Ppp3ca*)11255Kndl/J 017332 B6.Cg-Tg(tetO-Ppp3ca*)13967Kndl/J 017330 B6.Cg-Tg(tetO-TAg*)175Kndl/J 006234 B6.Cg-Tg(tetO-cre)1Jaw/J 023757 B6.Cg-Tg(tetO-tetX,lacZ)1Gogo/UmriJ 005738 B6.FVB-Tg(tetO-EGFP,-Tgfbr2)8Mcle/J 021025 B6;129-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm1(tetO-cre)Haho/J 006911 B6;129-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm2(tetO-Pou5f1)Jae/J 011001 B6;129S4-Col1a1tm1(tetO-Pou5f1,-Klf4,-Sox2,-Myc)Hoch/J 016836 B6;129S4-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm7(tetO-HIST1H2BJ/GFP)Jae/J 012433 B6;C3-Tg(ACTA1-rtTA,tetO-cre)102Monk/J 002709 B6;C3-Tg(TettTALuc)1Dgs/J 023598 B6;C3-Tg(tetO-AIMP2)630Tmd/J 023642 B6;C3-Tg(tetO-AIMP2)634Tmd/J 016841 B6;C3-Tg(tetO-TARDBP)12Vle/J 014650 B6;C3-Tg(tetO-TARDBP*)4Vle/J 012450 B6;D2-Tg(tetO-SNCA)1Cai/J 008344 B6;DBA-Tg(Fos-tTA,Fos-EGFP*)1Mmay Tg(tetO-lacZ,tTA*)1Mmay/J 024742 B6;DBA-Tg(tetO-GCaMP6s)2Niell/J 024088 B6;FVB-Tg(tetO-AML1/ETO)8Dzh/J 008082 B6;SJL-Tg(Tagln-tTA)1Mrab Tg(tetO-Mcpt1)1Mrab/J 010575 B6;SJL-Tg(tetO-Egfr*)2-9Jek/J 010577 B6;SJL-Tg(tetO-Erbb2*)8-4Jek/J 002621 B6;SJL-Tg(tetop-lacZ)2Mam/J 006004 B6C3-Tg(tetO-APPSwInd)885Dbo/Mmjax 016976 B6C3-Tg(tetO-SNCA*A53T)33Vle/J 018913 B6N.Cg-Tg(tetO-GFP,-lacZ)G3Rsp/J 006244 C.Cg-Tg(tetO-cre)1Jaw/J 017719 C3HeB/FeJ-Tg(tetO-TAg)1Efr/J 017955 C57BL/6-Tg(Gfap-rtTA,tetO-MAOB,-lacZ)1Jkan/J 025487 C57BL/6-Tg(tetO-Aimp1)29872Mcla/J 005706 C57BL/6-Tg(tetO-CDK5R1/GFP)337Lht/J 006618 C57BL/6-Tg(tetO-COX8A/EYFP)1Ksn/J 017613 C57BL/6-Tg(tetO-Cdkn1b)1Scpr/J 013729 C57BL/6-Tg(tetO-EDN1,-lacZ)9Mhus/J 016260 C57BL/6-Tg(tetO-Fbxl21)38Jt/J 016179 C57BL/6-Tg(tetO-Fbxl21*)11Jt/J 010713 C57BL/6-Tg(tetO-GFP/tetX)5696Stl/J 013728 C57BL/6-Tg(tetO-NOS2,-lacZ)240iMhus/J 016181 C57BL/6-Tg(tetO-Nr1d1)1Schb/J 016581 C57BL/6J-Tg(tetO-Btrc*)1Jt/J 008278 C57BL/6J-Tg(tetO-Clock)1Jt/J 024898 C57BL/6J-Tg(tetO-EGFP/Rpl10a)5aReij/J 016580 C57BL/6J-Tg(tetO-Usf1)2Jt/J 021065 FVB(C)-Tg(tetO-Npc1/YFP)1Mps/J 017542 FVB-Tg(Myh6/tetO-ATP2B4)1Jmol/J 016571 FVB-Tg(Myh6/tetO-Gata6)2Jmol/J 014155 FVB-Tg(Myh6/tetO-Itpr1)22.3Jmol/J 014153 FVB-Tg(Myh6/tetO-Itpr2)3.11Jmol/J 014154 FVB-Tg(Myh6/tetO-Itpr2)4.9Jmol/J 012684 FVB-Tg(Myh6/tetO-POSTN)22.1Jmol/J 010580 FVB-Tg(Myh6/tetO-PRKCA*)1Jmk/J 013156 FVB-Tg(tetO-CDK5R1*)1Vln/J 013777 FVB-Tg(tetO-Cacna1g)1Jmol/J 013778 FVB-Tg(tetO-Cacnb2)1Jmol/J 013779 FVB-Tg(tetO-Cacnb2)2Jmol/J 013780 FVB-Tg(tetO-Cib1)1Jmol/J 010578 FVB-Tg(tetO-Dusp6)1Jmol/J 017333 FVB-Tg(tetO-Gnai2*,-lacZ)382Kndl/J 008685 FVB-Tg(tetO-Kdr*)4377.5Rwng/J 023397 FVB-Tg(tetO-Lmnb1)AF1Yfu/J 015815 FVB-Tg(tetO-MAPT*P301L)#Kha/JlwsJ 008695 FVB-Tg(tetO-MET)23Rwng/J 012387 FVB-Tg(tetO-Ppargc1a)1Dpk/J 012385 FVB-Tg(tetO-Ppargc1b)7Dpk/J 022979 FVB-Tg(tetO-Thbs4)17.7Jmol/J 006439 FVB-Tg(tetO/CMV-KRAS*G12C)9.1Msmi/J 019038 FVB.Cg-Tg(tetO-GLI1)10Rup/Mmjax 019039 FVB.Cg-Tg(tetO-KLF4)32831Rup/Mmjax 008244 FVB.Cg-Tg(tetO-cre)1Jaw/J 012459 FVB/N-Tg(Myh6*/tetO-Capn1)L2Gwd/J 005941 FVB/N-Tg(tetO-Aurkb,lacZ)41Kra/J 006202 FVB/N-Tg(tetO-BCR/ABL1)2Dgt/J 014547 FVB/N-Tg(tetO-Fasl)BDepa/J 025672 FVB/N-Tg(tetO-Fgfr2b/Igh)1.3Jaw/J 019376 FVB/N-Tg(tetO-MYC)36aBop/J 023410 FVB/N-Tg(tetO-Nog,-EGFP)53Hda/J 026278 FVB/N-Tg(tetO-Ube3a*1)1Svd/J 026279 FVB/N-Tg(tetO-Ube3a*2)884Svd/J 022938 FVB/N-Tg(tetO-Wnt5a)17Rva/J 025104 FVB/N-Tg(tetO/Prnp-MAPT*P301L,-luc)Y74Dbo/J 003315 FVB/N-Tg(tetORo1-lacZ)3Conk/J 005076 NOD.Cg-Tg(tetO-EGFP/FADD)1Doi/DoiJ 006999 STOCK Dbttm1Geh Tg(Cebpb-tTA)5Bjd Tg(tetO-DBT)A1Geh/J 011004 STOCK Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm3(tetO-Pou5f1,-Sox2,-Klf4,-Myc)Jae/J 011011 STOCK Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm4(tetO-Pou5f1,-Sox2,-Klf4,-Myc)Jae/J 011013 STOCK Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm5(tetO-Pou5f1,-Klf4,-Myc)Jae/J 017596 STOCK Gt(ROSA)26Sortm1.1(rtTA,EGFP)Nagy Tg(SMN2)89Ahmb Smn1tm1Msd Tg(SMN2*delta7)4299Ahmb Tg(tetO-SMN2,-luc)#aAhmb/J 017597 STOCK Gt(ROSA)26Sortm1.1(rtTA,EGFP)Nagy Tg(SMN2)89Ahmb Smn1tm1Msd Tg(SMN2*delta7)4299Ahmb Tg(tetO-SMN2,-luc)#bAhmb/J 025671 STOCK Gt(ROSA)26Sortm1.1(rtTA,EGFP)Nagy Tg(tetO-Fgf10)1Jaw/SpdlJ 024854 STOCK Tg(Camk2a-tTA)1Mmay Tg(tetO-MAPT*P301L)#Kha/J 008755 STOCK Tg(Ins2-rtTA)2Efr Tg(teto-DTA)1Gfi/J 012477 STOCK Tg(Myh6*/tetO-GCaMP2)1Mik/J 016572 STOCK Tg(Myh6/tetO-Gata4)1Jmol/J 014544 STOCK Tg(tetO-ABL1*P242E*P249E)CPdav/J 014093 STOCK Tg(tetO-CHRM3*)1Blr/J 008790 STOCK Tg(tetO-DISC1*)1001Plet/J 008168 STOCK Tg(tetO-DTA)1Gfi/J 017755 STOCK Tg(tetO-GCAMP2)12iRyu/J 024509 STOCK Tg(tetO-Gata6)1Abl/J 016970 STOCK Tg(tetO-HCV)1Mlch/Mmjax 005104 STOCK Tg(tetO-HIST1H2BJ/GFP)47Efu/J 005699 STOCK Tg(tetO-Ipf1,EGFP)956.6Macd/J 005728 STOCK Tg(tetO-Ipf1,lacZ)958.1Macd/J 012441 STOCK Tg(tetO-LRRK2*G2019S)E3Cai/J 017918 STOCK Tg(tetO-MAML1*/EGFP)2Akar/J 017599 STOCK Tg(tetO-SMN2,-luc)#aAhmb/J 017600 STOCK Tg(tetO-SMN2,-luc)#bAhmb/J 012442 STOCK Tg(tetO-SNCA*A53T)E2Cai/J 006224 STOCK Tg(tetO-cre)1Jaw/J 017906 STOCK Tg(tetO-hop/EGFP,-COP4/mCherry)6Kftnk/J 012345 STOCK Tg(tetO-tdTomato,-Syp/EGFP*)1.1Luo/J 012449 STOCK Tg(teto-LRRK2)C7874Cai/JView Strains carrying other alleles of tetO (140 strains)
View Related Disease (OMIM) TermsRelated Disease (OMIM) Terms provided by MGI
- Potential model based on transgenic expression of an ortholog of a human gene that is associated with this disease. Phenotypic similarity to the human disease has not been tested.View Research ApplicationsResearch ApplicationsThis mouse can be used to support research in many areas including:
Tetop Tet System
Tet Expression Systems
tTA/rtTA Responsive Strains
|Allele Name||transgene insertion 279, Douglas Arenberg|
|Allele Type||Transgenic (Inducible, Inserted expressed sequence)|
|Strain of Origin||C57BL/6 x SJL|
|Expressed Gene||Mif, macrophage migration inhibitory factor, mouse, laboratory|
|Promoter||tetO, tet operator,|
|Molecular Note||Mouse Mif cDNA isolated from C57BL/6-derived Lewis lung carcinoma (LLC) cells was inserted into an expression cassette consisting of seven tetO repeats, a cytomegalovirus minimal promoter, and a bovine growth hormone polyadenylation sequence. Line 279 was generated. [MGI Ref ID J:191115]|
Animal Health ReportsProduction of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.
Breeding & Husbandry Hemizygotes and homozygotes are viable and fertile. The coat color of hemizygous and wildtype mice may be either black or agouti.
|Pricing for USA, Canada and Mexico shipping destinations|
Cryopreserved Mice - Ready for Recovery
Price (US dollars $) Cryorecovery* $2140.00
At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
|Pricing for International shipping destinations|
Cryopreserved Mice - Ready for Recovery
Price (US dollars $) Cryorecovery* $2782.00
Cryorecovery - Standard.
Progeny testing is not required.
|Considerations for Choosing Controls|
|Control Pricing Information for Genetically Engineered Mutant Strains.|
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