Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Heterozygote x Heterozygote         (Female x Male)   27-MAR-13 Species laboratory mouse Generation N4pN1+ (18-APR-13) Generation Definitions   Donating Investigator Caius Radu,   University of California, Los Angeles

Description
These Dck^flox/flox mutant mice possess loxP sites flanking exon 3, encoding the catalytic domain, of the deoxycytidine kinase (Dck) gene. Deoxycytidine kinase is a gene involved in deoxyribonucleoside salvage metabolism for use in DNA replication. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissues.

For example, when bred to B6.C-Tg(CMV-cre)1Cgn/J mice (Stock No. 006054), resulting Dck deficient offspring exhibit severe defects in T cell, B cell, and erythroid development resulting from acute cell cycle arrest in early S-phase in rapidly proliferating progenitor populations.

Development
A targeting vector was designed to insert a loxP sites flanking exon 3 of the deoxycytidine kinase (Dck) gene, followed by a frt-flanked neomycin resistance (neo) cassette. The construct was electroporated into 129S4/SvJae-derived LW-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to C57BL/6NTac mice. Offspring were bred with B6.Cg-Tg(ACTFLPe)9205Dym/J transgenic mice (Stock No. 005703) to delete the neo cassette, and progeny were crossed to remove the Flp-expressing transgene. These mice were bred to C57BL/6NTac mice for at least 4 more generations. Upon arrival, mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for at least one generation to establish the colony.

Control Information

	Control
	Heterozygote from the colony
	Wild-type from the colony
	005304 C57BL/6NJ	(approximate)
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Dck^tm1.2Radu/Dck^tm1.2Radu

        involves: 129S4/SvJae * C57BL/6 * C57BL/6N * SJL

• normal phenotype
• no abnormal phenotype detected
  • mice are viable and fertile   (MGI Ref ID J:101977)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cell Biology Research
Cell Cycle Regulation

Research Tools
Cre-lox System
      loxP-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Dcktm1.2Radu
Allele Name		targeted mutation 1.2, Caius G Radu
Allele Type		Targeted (Floxed/Frt)
Common Name(s)		Dckflox;
Strain of Origin		129S4/SvJae
Gene Symbol and Name		Dck, deoxycytidine kinase
Chromosome		5
Molecular Note		A targeting vector was designed to insert a loxP sites flanking exon 3, followed by a frt-flanked neomycin resistance (neo) cassette. Flp-mediated recombination remvoed the neo cassette and left exon 3 floxed. [MGI Ref ID J:158373]

Genotyping

Genotyping Information

Genotyping Protocols

Dck^tm1.2Radu, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Toy G; Austin WR; Liao HI; Cheng D; Singh A; Campbell DO; Ishikawa TO; Lehmann LW; Satyamurthy N; Phelps ME; Herschman HR; Czernin J; Witte ON; Radu CG. 2010. Requirement for deoxycytidine kinase in T and B lymphocyte development. Proc Natl Acad Sci U S A 107(12):5551-6. [PubMed: 20080663]  [MGI Ref ID J:158373]

Additional References

Dck^tm1.2Radu related

The Jackson Laboratory. 2005. Information obtained from The Jackson Laboratory, Bar Harbor, ME Unpublished :.  [MGI Ref ID J:101977]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           FGB29

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, homozygous mice may be bred together.
Mating System	Heterozygote x Heterozygote         (Female x Male)   27-MAR-13
Diet Information	LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

 

This strain is currently Under Development - Now Accepting Orders.
Estimated Available for Distribution Date: 15-JUL-13

Please note: You may now place orders for this strain although it is not yet ready for distribution. Estimated available for distribution dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for distribution depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain.

Control Information

	Control
	Heterozygote from the colony
	Wild-type from the colony
	005304 C57BL/6NJ	(approximate)
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Terms of Use

Terms of Use

General Terms and Conditions

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Contact information

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