Strain Name:

B6;129-Nrltm1Asw/J

Stock Number:

021152

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Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
Nrl (neural retina leucine zipper gene) plays an essential role in rod photoreceptor differentiation and homeostasis. These animals carry a targeted allele in which the entire coding region of the mouse gene has been deleted. This strain may be useful in studies of retinal and macular degenerative diseases.

Description

Strain Information

Former Names B6.129-Nrltm1Asw/J    (Changed: 30-JAN-14 )
Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Mating SystemHeterozygote x Heterozygote         (Female x Male)   10-JUL-13
Specieslaboratory mouse
GenerationN4+pN1 (10-OCT-13)
Generation Definitions
 
Donating Investigator Anand Swaroop,   NIH/NEI

Description
Nrl (neural retina leucine zipper gene) encodes a retinal transcription factor that plays an essential role in rod photoreceptor differentiation and homeostasis. Dysfunction or death of rod photoreceptors precedes cone loss in many retinal and macular degenerative diseases.

These animals carry a targeted allele in which the entire coding region of the mouse gene has been deleted. Homozyous knockouts are morphologically normal, viable and fertile, but show a complete loss of rod function and super-normal cone function. The photoreceptors have cone-like nuclear morphology and short, sparse outer segments with abnormal disks. Retinas undergo a rapid but transient period of degeneration in early adulthood, with cone apoptosis, retinal detachment, alterations in retinal vessel structure, and activation and translocation of retinal microglia. Cone degeneration stabilizes by 4 months of age, resulting in a thinner but intact outer nuclear layer with residual cones expressing S- and M-opsins and preserved photopic electroretinogram.

Homozyous Nrl mutant mice show no Nr2e3 (nuclear receptor subfamily 2, group E, member 3) expression in the retina, demonstrating that Nrl is upstream in pathways leading the development of photoreceptors.

Development
The entire coding region (exons 2 and 3) was replaced with a neomycin resistance cassette. R1 embryonic stem (ES) cells derived from (129X1/SvJ x 129S1/Sv)F1- Kitl+ were used to create the mutation. The donating investigator reported that this strain was backcrossed to C57BL/6J for at least 4-5 generations prior to arrival at The Jackson Laboratory (see SNP note below).

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. Tweleve markers throughout the genome are segregating for 129 suggesting an incomplete backcross.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Nrl
021232   B6.Cg-Tg(Nrl-EGFP)1Asw/J
View Strains carrying other alleles of Nrl     (1 strain)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Neural Retina Leucine Zipper; NRL   (NRL)
Retinitis Pigmentosa 27; RP27   (NRL)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Nrltm1Asw/Nrltm1Asw

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
  • vision/eye phenotype
  • abnormal eye physiology
    • overexpress S opsin with an abnormal distribution   (MGI Ref ID J:174592)
    • abnormal cone electrophysiology
      • in light-adapted conditions, the b-wave threshold was the same for wild-type and mutant mice, indicating a functional cone pathway in the mutant retina   (MGI Ref ID J:78692)
      • notably, the amplitude of the maximum light-adapted ERG b-wave for mutant mice was 2-3-fold larger relative to wild-type, indicating enhanced cone-mediated activity   (MGI Ref ID J:78692)
      • ERGs using monochromatic stimuli revealed that the amplitude of the S cone-mediated response was >6 times larger for mutant mice than for wild-type, suggesting a super-normal S-cone function; in contrast, the M-cone response was relatively unaffected   (MGI Ref ID J:78692)
    • abnormal rod electrophysiology
      • ERGs from dark-adapted mutant mice revealed a complete absence of rod function ("pure-cone" retinas)   (MGI Ref ID J:78692)
      • lack scotopic ERG responses   (MGI Ref ID J:174592)
  • abnormal retinal outer nuclear layer morphology
    • at 5 weeks, the outer nuclear layer (ONL) of the mutant retina had a normal thickness and number of nuclei but appeared to be disrupted with whorls and rosettes   (MGI Ref ID J:78692)
    • by 31 weeks, the rosettes and whorls were no longer detectable and thinning of the ONL had occurred   (MGI Ref ID J:78692)
  • abnormal retinal photoreceptor morphology
    • analysis of "pure-cone" retinas demonstrated that both S and M cone opsins were phosphorylated after light exposure and that cone arrestin selectively bound to light-activated, phosphorylated cone opsins   (MGI Ref ID J:84455)
    • most mutant photoreceptor nuclei were ellipsoid and showed a distributed pattern of heterochromatin, characteristic of cones   (MGI Ref ID J:78692)
    • immunohistochemistry revealed normal M-opsin distribution but no rhodopsin immunoreactivity; S-opsin was detected throughout the outer segments, indicating a loss of rod and 'gain' of S- but not M-cone activity   (MGI Ref ID J:78692)
    • abnormal photoreceptor outer segment morphology
      • the outer segments were significantly fewer and shorter and showed abnormal disk morphology; also, the outer segment disks were often misaligned and abnormally associated with the retinal pigment epithelium   (MGI Ref ID J:78692)
  • nervous system phenotype
  • abnormal retinal photoreceptor morphology
    • analysis of "pure-cone" retinas demonstrated that both S and M cone opsins were phosphorylated after light exposure and that cone arrestin selectively bound to light-activated, phosphorylated cone opsins   (MGI Ref ID J:84455)
    • most mutant photoreceptor nuclei were ellipsoid and showed a distributed pattern of heterochromatin, characteristic of cones   (MGI Ref ID J:78692)
    • immunohistochemistry revealed normal M-opsin distribution but no rhodopsin immunoreactivity; S-opsin was detected throughout the outer segments, indicating a loss of rod and 'gain' of S- but not M-cone activity   (MGI Ref ID J:78692)
    • abnormal photoreceptor outer segment morphology
      • the outer segments were significantly fewer and shorter and showed abnormal disk morphology; also, the outer segment disks were often misaligned and abnormally associated with the retinal pigment epithelium   (MGI Ref ID J:78692)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Nrltm1Asw/Nrltm1Asw

        involves: 129S1/Sv * 129X1/SvJ
  • vision/eye phenotype
  • abnormal retinal photoreceptor morphology
    • rods are reprogrammed into cones   (MGI Ref ID J:193697)
  • nervous system phenotype
  • abnormal retinal photoreceptor morphology
    • rods are reprogrammed into cones   (MGI Ref ID J:193697)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Sensorineural Research
Eye Defects
Retinal Degeneration

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Nrltm1Asw
Allele Name targeted mutation 1, Anand Swaroop
Allele Type Targeted (knock-out)
Common Name(s) Nrl-; cone-full;
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name Nrl, neural retina leucine zipper gene
Chromosome 14
Gene Common Name(s) AW492574; D14H14S46E; D14S46E; DNA segment, Chr 14, human D14S46E; NRL-MAF; RP27; expressed sequence AW492574;
Molecular Note A PGK-neomycin resistance cassette replaced the entire coding region (exons 2 and 3). Immunoblot analysis did not detect protein in retina from 10 day old homozygous mutant mice. RT-PCR studies of retina from 10 day old homozygous mutant mice did not detect mRNA. [MGI Ref ID J:78692]

Genotyping

Genotyping Information

Genotyping Protocols

Nrltm1Aswalternate2, Standard PCR
Ptprcab End Point, End Point Analysis


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Swain PK; Hicks D; Mears AJ; Apel IJ; Smith JE; John SK; Hendrickson A; Milam AH; Swaroop A. 2001. Multiple phosphorylated isoforms of NRL are expressed in rod photoreceptors. J Biol Chem 276(39):36824-30. [PubMed: 11477108]  [MGI Ref ID J:71808]

Additional References

Nrltm1Asw related

Akimoto M; Cheng H; Zhu D; Brzezinski JA; Khanna R; Filippova E; Oh EC; Jing Y; Linares JL; Brooks M; Zareparsi S; Mears AJ; Hero A; Glaser T; Swaroop A. 2006. Targeting of GFP to newborn rods by Nrl promoter and temporal expression profiling of flow-sorted photoreceptors. Proc Natl Acad Sci U S A 103(10):3890-5. [PubMed: 16505381]  [MGI Ref ID J:107155]

Chakraborty D; Conley SM; Stuck MW; Naash MI. 2010. Differences in RDS trafficking, assembly and function in cones versus rods: insights from studies of C150S-RDS. Hum Mol Genet 19(24):4799-812. [PubMed: 20858597]  [MGI Ref ID J:166363]

Chen C; Blakeley LR; Koutalos Y. 2009. Formation of all-trans retinol after visual pigment bleaching in mouse photoreceptors. Invest Ophthalmol Vis Sci 50(8):3589-95. [PubMed: 19264891]  [MGI Ref ID J:154568]

Cheng H; Aleman TS; Cideciyan AV; Khanna R; Jacobson SG; Swaroop A. 2006. In vivo function of the orphan nuclear receptor NR2E3 in establishing photoreceptor identity during mammalian retinal development. Hum Mol Genet 15(17):2588-602. [PubMed: 16868010]  [MGI Ref ID J:114905]

Cheng H; Khan NW; Roger JE; Swaroop A. 2011. Excess cones in the retinal degeneration rd7 mouse, caused by the loss of function of orphan nuclear receptor Nr2e3, originate from early-born photoreceptor precursors. Hum Mol Genet 20(21):4102-15. [PubMed: 21813656]  [MGI Ref ID J:176686]

Chrispell JD; Feathers KL; Kane MA; Kim CY; Brooks M; Khanna R; Kurth I; Hubner CA; Gal A; Mears AJ; Swaroop A; Napoli JL; Sparrow JR; Thompson DA. 2009. Rdh12 activity and effects on retinoid processing in the murine retina. J Biol Chem 284(32):21468-77. [PubMed: 19506076]  [MGI Ref ID J:153181]

Cideciyan AV; Rachel RA; Aleman TS; Swider M; Schwartz SB; Sumaroka A; Roman AJ; Stone EM; Jacobson SG; Swaroop A. 2011. Cone photoreceptors are the main targets for gene therapy of NPHP5 (IQCB1) or NPHP6 (CEP290) blindness: generation of an all-cone Nphp6 hypomorph mouse that mimics the human retinal ciliopathy. Hum Mol Genet 20(7):1411-23. [PubMed: 21245082]  [MGI Ref ID J:169232]

Conley SM; Cai X; Makkia R; Wu Y; Sparrow JR; Naash MI. 2012. Increased cone sensitivity to ABCA4 deficiency provides insight into macular vision loss in Stargardt's dystrophy. Biochim Biophys Acta 1822(7):1169-79. [PubMed: 22033104]  [MGI Ref ID J:185019]

Corbo JC; Myers CA; Lawrence KA; Jadhav AP; Cepko CL. 2007. A typology of photoreceptor gene expression patterns in the mouse. Proc Natl Acad Sci U S A 104(29):12069-74. [PubMed: 17620597]  [MGI Ref ID J:123149]

Dang L; Pulukuri S; Mears AJ; Swaroop A; Reese BE; Sitaramayya A. 2004. Connexin 36 in photoreceptor cells: studies on transgenic rod-less and cone-less mouse retinas. Mol Vis 10:323-7. [PubMed: 15152186]  [MGI Ref ID J:90715]

Daniele LL; Adams RH; Durante DE; Pugh EN Jr; Philp NJ. 2007. Novel distribution of junctional adhesion molecule-C in the neural retina and retinal pigment epithelium. J Comp Neurol 505(2):166-76. [PubMed: 17853450]  [MGI Ref ID J:137255]

Daniele LL; Lillo C; Lyubarsky AL; Nikonov SS; Philp N; Mears AJ; Swaroop A; Williams DS; Pugh EN Jr. 2005. Cone-like morphological, molecular, and electrophysiological features of the photoreceptors of the Nrl knockout mouse. Invest Ophthalmol Vis Sci 46(6):2156-67. [PubMed: 15914637]  [MGI Ref ID J:99413]

Ding XQ; Fitzgerald JB; Matveev AV; McClellan ME; Elliott MH. 2008. Functional activity of photoreceptor cyclic nucleotide-gated channels is dependent on the integrity of cholesterol- and sphingolipid-enriched membrane domains. Biochemistry 47(12):3677-87. [PubMed: 18303857]  [MGI Ref ID J:133081]

Ding XQ; Harry CS; Umino Y; Matveev AV; Fliesler SJ; Barlow RB. 2009. Impaired cone function and cone degeneration resulting from CNGB3 deficiency: down-regulation of CNGA3 biosynthesis as a potential mechanism. Hum Mol Genet 18(24):4770-4780. [PubMed: 19767295]  [MGI Ref ID J:154304]

Egger A; Samardzija M; Sothilingam V; Tanimoto N; Lange C; Salatino S; Fang L; Garcia-Garrido M; Beck S; Okoniewski MJ; Neutzner A; Seeliger MW; Grimm C; Handschin C. 2012. PGC-1alpha determines light damage susceptibility of the murine retina. PLoS One 7(2):e31272. [PubMed: 22348062]  [MGI Ref ID J:185229]

Farjo R; Fliesler SJ; Naash MI. 2007. Effect of Rds abundance on cone outer segment morphogenesis, photoreceptor gene expression, and outer limiting membrane integrity. J Comp Neurol 504(6):619-30. [PubMed: 17722028]  [MGI Ref ID J:131880]

Farjo R; Skaggs JS; Nagel BA; Quiambao AB; Nash ZA; Fliesler SJ; Naash MI. 2006. Retention of function without normal disc morphogenesis occurs in cone but not rod photoreceptors. J Cell Biol 173(1):59-68. [PubMed: 16585269]  [MGI Ref ID J:107832]

Feathers KL; Lyubarsky AL; Khan NW; Teofilo K; Swaroop A; Williams DS; Pugh EN Jr; Thompson DA. 2008. Nrl-knockout mice deficient in Rpe65 fail to synthesize 11-cis retinal and cone outer segments. Invest Ophthalmol Vis Sci 49(3):1126-35. [PubMed: 18326740]  [MGI Ref ID J:133023]

Fischer MD; Huber G; Beck SC; Tanimoto N; Muehlfriedel R; Fahl E; Grimm C; Wenzel A; Reme CE; van de Pavert SA; Wijnholds J; Pacal M; Bremner R; Seeliger MW. 2009. Noninvasive, in vivo assessment of mouse retinal structure using optical coherence tomography. PLoS One 4(10):e7507. [PubMed: 19838301]  [MGI Ref ID J:154049]

Hackler L Jr; Wan J; Swaroop A; Qian J; Zack DJ. 2010. MicroRNA profile of the developing mouse retina. Invest Ophthalmol Vis Sci 51(4):1823-31. [PubMed: 19933188]  [MGI Ref ID J:160406]

Kanan Y; Kasus-Jacobi A; Moiseyev G; Sawyer K; Ma JX; Al-Ubaidi MR. 2008. Retinoid processing in cone and Muller cell lines. Exp Eye Res 86(2):344-54. [PubMed: 18163989]  [MGI Ref ID J:132505]

Kolandaivelu S; Chang B; Ramamurthy V. 2011. Rod Phosphodiesterase-6 (PDE6) Catalytic Subunits Restore Cone Function in a Mouse Model Lacking Cone PDE6 Catalytic Subunit. J Biol Chem 286(38):33252-9. [PubMed: 21799013]  [MGI Ref ID J:176734]

Kolandaivelu S; Huang J; Hurley JB; Ramamurthy V. 2009. AIPL1, a protein associated with childhood blindness, interacts with alpha-subunit of rod phosphodiesterase (PDE6) and is essential for its proper assembly. J Biol Chem 284(45):30853-61. [PubMed: 19758987]  [MGI Ref ID J:156330]

Kolandaivelu S; Singh RK; Ramamurthy V. 2014. AIPL1, A protein linked to blindness, is essential for the stability of enzymes mediating cGMP metabolism in cone photoreceptor cells. Hum Mol Genet 23(4):1002-12. [PubMed: 24108108]  [MGI Ref ID J:205988]

Krigel A; Felder-Schmittbuhl MP; Hicks D. 2010. Circadian-clock driven cone-like photoreceptor phagocytosis in the neural retina leucine zipper gene knockout mouse. Mol Vis 16:2873-81. [PubMed: 21203345]  [MGI Ref ID J:168341]

Kunchithapautham K; Coughlin B; Crouch RK; Rohrer B. 2009. Cone outer segment morphology and cone function in the Rpe65-/- Nrl-/- mouse retina are amenable to retinoid replacement. Invest Ophthalmol Vis Sci 50(10):4858-64. [PubMed: 19407011]  [MGI Ref ID J:154550]

Liu C; Lin C; Whitaker DT; Bakeri H; Bulgakov OV; Liu P; Lei J; Dong L; Li T; Swaroop A. 2013. Prickle1 is expressed in distinct cell populations of the central nervous system and contributes to neuronal morphogenesis. Hum Mol Genet 22(11):2234-46. [PubMed: 23420014]  [MGI Ref ID J:198157]

Lobanova ES; Herrmann R; Finkelstein S; Reidel B; Skiba NP; Deng WT; Jo R; Weiss ER; Hauswirth WW; Arshavsky VY. 2010. Mechanistic basis for the failure of cone transducin to translocate: why cones are never blinded by light. J Neurosci 30(20):6815-24. [PubMed: 20484624]  [MGI Ref ID J:160517]

Mears AJ; Kondo M; Swain PK; Takada Y; Bush RA; Saunders TL; Sieving PA; Swaroop A. 2001. Nrl is required for rod photoreceptor development. Nat Genet 29(4):447-52. [PubMed: 11694879]  [MGI Ref ID J:78692]

Montana CL; Kolesnikov AV; Shen SQ; Myers CA; Kefalov VJ; Corbo JC. 2013. Reprogramming of adult rod photoreceptors prevents retinal degeneration. Proc Natl Acad Sci U S A 110(5):1732-7. [PubMed: 23319618]  [MGI Ref ID J:193697]

Mustafi D; Kevany BM; Genoud C; Okano K; Cideciyan AV; Sumaroka A; Roman AJ; Jacobson SG; Engel A; Adams MD; Palczewski K. 2011. Defective photoreceptor phagocytosis in a mouse model of enhanced S-cone syndrome causes progressive retinal degeneration. FASEB J 25(9):3157-76. [PubMed: 21659555]  [MGI Ref ID J:175566]

Nasonkin IO; Lazo K; Hambright D; Brooks M; Fariss R; Swaroop A. 2011. Distinct nuclear localization patterns of DNA methyltransferases in developing and mature mammalian retina. J Comp Neurol 519(10):1914-30. [PubMed: 21452232]  [MGI Ref ID J:180117]

Ng L; Lu A; Swaroop A; Sharlin DS; Swaroop A; Forrest D. 2011. Two transcription factors can direct three photoreceptor outcomes from rod precursor cells in mouse retinal development. J Neurosci 31(31):11118-25. [PubMed: 21813673]  [MGI Ref ID J:174592]

Nikonov SS; Daniele LL; Zhu X; Craft CM; Swaroop A; Pugh EN Jr. 2005. Photoreceptors of Nrl -/- mice coexpress functional S- and M-cone opsins having distinct inactivation mechanisms. J Gen Physiol 125(3):287-304. [PubMed: 15738050]  [MGI Ref ID J:112480]

Oh EC; Khan N; Novelli E; Khanna H; Strettoi E; Swaroop A. 2007. Transformation of cone precursors to functional rod photoreceptors by bZIP transcription factor NRL. Proc Natl Acad Sci U S A 104(5):1679-84. [PubMed: 17242361]  [MGI Ref ID J:119504]

Peng GH; Chen S. 2011. Active opsin loci adopt intrachromosomal loops that depend on the photoreceptor transcription factor network. Proc Natl Acad Sci U S A 108(43):17821-6. [PubMed: 22006320]  [MGI Ref ID J:177494]

Peng GH; Chen S. 2007. Crx activates opsin transcription by recruiting HAT-containing co-activators and promoting histone acetylation. Hum Mol Genet 16(20):3433-52. [PubMed: 17656371]  [MGI Ref ID J:129889]

Rajala A; Dighe R; Agbaga MP; Anderson RE; Rajala RV. 2013. Insulin receptor signaling in cones. J Biol Chem 288(27):19503-15. [PubMed: 23673657]  [MGI Ref ID J:199655]

Raven MA; Oh EC; Swaroop A; Reese BE. 2007. Afferent control of horizontal cell morphology revealed by genetic respecification of rods and cones. J Neurosci 27(13):3540-7. [PubMed: 17392470]  [MGI Ref ID J:120129]

Roger JE; Hiriyanna A; Gotoh N; Hao H; Cheng DF; Ratnapriya R; Kautzmann MA; Chang B; Swaroop A. 2014. OTX2 loss causes rod differentiation defect in CRX-associated congenital blindness. J Clin Invest :. [PubMed: 24382353]  [MGI Ref ID J:203337]

Roger JE; Ranganath K; Zhao L; Cojocaru RI; Brooks M; Gotoh N; Veleri S; Hiriyanna A; Rachel RA; Campos MM; Fariss RN; Wong WT; Swaroop A. 2012. Preservation of Cone Photoreceptors after a Rapid yet Transient Degeneration and Remodeling in Cone-Only Nrl-/- Mouse Retina. J Neurosci 32(2):528-41. [PubMed: 22238088]  [MGI Ref ID J:179907]

Strettoi E; Mears AJ; Swaroop A. 2004. Recruitment of the rod pathway by cones in the absence of rods. J Neurosci 24(34):7576-82. [PubMed: 15329405]  [MGI Ref ID J:97262]

Stuck MW; Conley SM; Naash MI. 2012. Defects in the outer limiting membrane are associated with rosette development in the Nrl-/- retina. PLoS One 7(3):e32484. [PubMed: 22427845]  [MGI Ref ID J:186920]

Thapa A; Morris L; Xu J; Ma H; Michalakis S; Biel M; Ding XQ. 2012. Endoplasmic reticulum stress-associated cone photoreceptor degeneration in cyclic nucleotide-gated channel deficiency. J Biol Chem 287(22):18018-29. [PubMed: 22493484]  [MGI Ref ID J:185617]

Wenzel A; von Lintig J; Oberhauser V; Tanimoto N; Grimm C; Seeliger MW. 2007. RPE65 is essential for the function of cone photoreceptors in NRL-deficient mice. Invest Ophthalmol Vis Sci 48(2):534-42. [PubMed: 17251447]  [MGI Ref ID J:123283]

Xu J; Morris L; Thapa A; Ma H; Michalakis S; Biel M; Baehr W; Peshenko IV; Dizhoor AM; Ding XQ. 2013. cGMP accumulation causes photoreceptor degeneration in CNG channel deficiency: evidence of cGMP cytotoxicity independently of enhanced CNG channel function. J Neurosci 33(37):14939-48. [PubMed: 24027293]  [MGI Ref ID J:202332]

Yao J; Feathers KL; Khanna H; Thompson D; Tsilfidis C; Hauswirth WW; Heckenlively JR; Swaroop A; Zacks DN. 2011. XIAP therapy increases survival of transplanted rod precursors in a degenerating host retina. Invest Ophthalmol Vis Sci 52(3):1567-72. [PubMed: 20926819]  [MGI Ref ID J:171553]

Yetemian RM; Brown BM; Craft CM. 2010. Neovascularization, enhanced inflammatory response, and age-related cone dystrophy in the Nrl-/-Grk1-/- mouse retina. Invest Ophthalmol Vis Sci 51(12):6196-206. [PubMed: 20688726]  [MGI Ref ID J:171388]

Yu J; He S; Friedman JS; Akimoto M; Ghosh D; Mears AJ; Hicks D; Swaroop A. 2004. Altered expression of genes of the Bmp/Smad and Wnt/calcium signaling pathways in the cone-only Nrl-/- mouse retina, revealed by gene profiling using custom cDNA microarrays. J Biol Chem 279(40):42211-20. [PubMed: 15292180]  [MGI Ref ID J:118580]

Zhu X; Brown B; Li A; Mears AJ; Swaroop A; Craft CM. 2003. GRK1-dependent phosphorylation of S and M opsins and their binding to cone arrestin during cone phototransduction in the mouse retina. J Neurosci 23(14):6152-60. [PubMed: 12853434]  [MGI Ref ID J:84455]

Zuniga FI; Craft CM. 2010. Deciphering the structure and function of als2cr4 in the mouse retina. Invest Ophthalmol Vis Sci 51(9):4407-15. [PubMed: 20375344]  [MGI Ref ID J:164092]

de Melo J; Peng GH; Chen S; Blackshaw S. 2011. The Spalt family transcription factor Sall3 regulates the development of cone photoreceptors and retinal horizontal interneurons. Development 138(11):2325-36. [PubMed: 21558380]  [MGI Ref ID J:173617]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX18

Colony Maintenance

Breeding & HusbandryHomozygotes and heterozygotes are viable and fertile.
Mating SystemHeterozygote x Heterozygote         (Female x Male)   10-JUL-13

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHeterozygous for Nrltm1Asw  
$232.00Female or MaleHomozygous for Nrltm1Asw  
Price per Pair (US dollars $)Pair Genotype
$464.00Heterozygous for Nrltm1Asw x Heterozygous for Nrltm1Asw  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHeterozygous for Nrltm1Asw  
$301.60Female or MaleHomozygous for Nrltm1Asw  
Price per Pair (US dollars $)Pair Genotype
$603.20Heterozygous for Nrltm1Asw x Heterozygous for Nrltm1Asw  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

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phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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