|Tau-lacZ targeting of the Opn4 (opsin 4 (melanopsin)) gene enables X-gal labeling of axons, cell bodies, and proximal dendrites of retinal ganglion cells that photoentrain light signals to the primary circadian pacemaker in the suprachiasmatic nucleus (SCN).|
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator King-Wai Yau, Johns Hopkins University School of Medicine
Opn4 (opsin 4 (melanopsin)) encodes a G-protein coupled receptor/photopigment found in mammalian retinal ganglion cells (RGCs) that photoentrain light signals from the eyes through the retinohypothalamic tract to the primary circadian pacemaker in the suprachiasmatic nucleus (SCN).
In this Opn4 knockout mutation, most of exons 1-9 and the intervening sequence are replaced by tau-lacZ. Tau-lacZ codes for a protein composed of the β-galactosidase enzyme fused to a signal sequence from tau (an actin-associated protein), which allows the fusion protein to be preferentially transported down axons to presynaptic terminals. In retinas from heterozygous animals, β-galactosidase and OPN4 immunoreactivities colocalize in ganglion cells. X-gal staining of β-galactosidase activity marks axons, cell bodies, and proximal dendrites are labeled.
Deletion of activity in homozygotes eliminates the intrinsic photosensitivity of retinal ganglion cells originally expressing melanopsin, leading to defects in various non-image-forming visual functions such as pupillary light reflex and circadian photoentrainment. The homozygotes are viable and fertile.
Tau-lacZ and a loxP-flanked pgk-neomycin cassette replace fragments of exons 1 and 9 and the intervening sequence. The mutation was created in 129.1 embryonic stem (ES) cells. Resulting chimeric animals were bred with C57BL/6 mice. The neomycin cassette has been excised. This strain was maintained on a unknown, mixed genetic background by the donating lab.
View Mammalian Phenotype TermsMammalian Phenotype Terms provided by MGIassigned by genotype
The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.
Opn4tm1.1Yau/Opn4tm1.1Yauinvolves: 129 * C57BL/6
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:101977)
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|Allele Name||targeted mutation 1.1, King-Wai Yau|
|Allele Type||Targeted (Null/Knockout, Reporter)|
|Strain of Origin||129|
|Gene Symbol and Name||Opn4, opsin 4 (melanopsin)|
|Gene Common Name(s)||1110007J02Rik; Gm533; MOP; RIKEN cDNA 1110007J02 gene; gene model 533, (NCBI);|
|Molecular Note||Tau-lacZ and a loxP-flanked pgk-neomycin cassette replace fragments of exons 1 and 9 and the intervening sequence. Cre-mediated recombination removed the neomycin cassette. [MGI Ref ID J:101977] [MGI Ref ID J:81604]|
Hattar S; Liao HW; Takao M; Berson DM; Yau KW. 2002. Melanopsin-containing retinal ganglion cells: architecture, projections, and intrinsic photosensitivity. Science 295(5557):1065-70. [PubMed: 11834834] [MGI Ref ID J:81604]
Jagannath A; Butler R; Godinho SI; Couch Y; Brown LA; Vasudevan SR; Flanagan KC; Anthony D; Churchill GC; Wood MJ; Steiner G; Ebeling M; Hossbach M; Wettstein JG; Duffield GE; Gatti S; Hankins MW; Foster RG; Peirson SN. 2013. The CRTC1-SIK1 pathway regulates entrainment of the circadian clock. Cell 154(5):1100-11. [PubMed: 23993098] [MGI Ref ID J:204540]
The Jackson Laboratory. 2005. Information obtained from The Jackson Laboratory, Bar Harbor, ME Unpublished :. [MGI Ref ID J:101977]
Breeding & Husbandry Homozygotes and heterozygotes are viable and fertile.
This strain is currently In Progress.View All Strains Awaiting Transfer from the Donor, In Progress and On Hold
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