Description

Strain Information

Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse   Donating Investigator Tej Pandita,   UTSouthwestern Medical Center

Description
These conditional mMof mice possess loxP sites flanking exons 1-3 of the K(lysine) acetyltransferase 8 (Kat8) gene. Kat8, also called mMOF (murine, males absent on the first), encodes a histone H4 lysine 16 (H4K16)-specific acetyltransferase. Acetylation of H4K16 is required for cellular growth and proliferation during embryogenesis and organogenesis. Overexpression of MOF results in increased H4K16ac levels, increased cellular proliferation, oncogenic transformation, and tumor growth. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 1-3 deleted in cre-expressing tissues. Complete mMOF deficiency results in embryonic lethality and cell death.

For example, when crossed to B6.129-Tg(Pcp2-cre)2Mpin/J mice (Stock No. 004146) expressing Cre recombinase in Purkinje cells, mMof-deficient mice display impaired motor coordination, ataxia, a backward-walking phenotype, and a reduced life span.

Development
A targeting vector was designed to insert a frt-flanked neomycin resistance (neo) cassette with a 5' loxP site upstream of exon 1, and a single loxP site downstream of exon 3 of the K(lysine) acetyltransferase 8 (Kat8) gene. The construct was electroporated into 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺-derived W9.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts and resulting chimeric males were bred with C57BL/6J females. These mMof mice were maintained on a mixed background. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Control Information

	Control
	101045 B6129SF2/J	(approximate)
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cell Biology Research
Genes Regulating Growth and Proliferation

Developmental Biology Research
Internal/Organ Defects

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Kat8tm1Thl
Allele Name		targeted mutation 1, Thomas Ludwig
Allele Type		Targeted (Floxed/Frt)
Common Name(s)		Mofflox; mMofflox;
Strain of Origin		129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
Gene Symbol and Name		Kat8, K(lysine) acetyltransferase 8
Chromosome		7
Gene Common Name(s)		2010203C02Rik; 5830450F21Rik; D7Ertd629e; DNA segment, Chr 7, ERATO Doi 629, expressed; MOF; MYST histone acetyltransferase 1; MYST1; Myst1; RIKEN cDNA 2010203C02 gene; RIKEN cDNA 5830450F21 gene; ZC2HC8; hMOF;
Molecular Note		An frt-flanked neo cassette with a 5' loxP site was inserted upstream of intron 1 and an additional loxP site was inserted downstream of exon 3. [MGI Ref ID J:128936]

Genotyping

Genotyping Information

Genotyping Protocols

Tg(ACTFLPe), High Resolution Melting

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Gupta A; Guerin-Peyrou TG; Sharma GG; Park C; Agarwal M; Ganju RK; Pandita S; Choi K; Sukumar S; Pandita RK; Ludwig T; Pandita TK. 2008. The mammalian ortholog of Drosophila MOF that acetylates histone H4 lysine 16 is essential for embryogenesis and oncogenesis. Mol Cell Biol 28(1):397-409. [PubMed: 17967868]  [MGI Ref ID J:128936]

Additional References

Kat8^tm1Thl related

Kumar R; Hunt CR; Gupta A; Nannepaga S; Pandita RK; Shay JW; Bachoo R; Ludwig T; Burns DK; Pandita TK. 2011. Purkinje cell-specific males absent on the first (mMof) gene deletion results in an ataxia-telangiectasia-like neurological phenotype and backward walking in mice. Proc Natl Acad Sci U S A 108(9):3636-41. [PubMed: 21321203]  [MGI Ref ID J:170483]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, mice homozygous for the floxed allele may be bred together.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

 

This strain is currently Awaiting Transfer from the Donor.

This strain was accepted into the JAX^® Repository on  13-FEB-13.

View All Strains Awaiting Transfer from the Donor, In Progress and On Hold

Control Information

	Control
	101045 B6129SF2/J	(approximate)
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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