Strain Name:

B6;129S1-Kat8tm1Thl/J

Stock Number:

021209

Availability:

On Hold

Use Restrictions Apply, see Terms of Use
Register Interest
These mMof floxed mutant mice possess loxP sites flanking exons 1-3 of the K(lysine) acetyltransferase 8 (Kat8) gene. This strain may be useful for studying the role of histone H4 acetylation of lysine 16 on embryogenesis and organogenesis.

Description

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Mating SystemHeterozygote x Heterozygote         (Female x Male)   10-JUL-13
Specieslaboratory mouse
 
Donating Investigator Tej Pandita,   UTSouthwestern Medical Center

Description
These conditional mMof mice possess loxP sites flanking exons 1-3 of the K(lysine) acetyltransferase 8 (Kat8) gene. Kat8, also called mMOF (murine, males absent on the first), encodes a histone H4 lysine 16 (H4K16)-specific acetyltransferase. Acetylation of H4K16 is required for cellular growth and proliferation during embryogenesis and organogenesis. Overexpression of MOF results in increased H4K16ac levels, increased cellular proliferation, oncogenic transformation, and tumor growth. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 1-3 deleted in cre-expressing tissues. Complete mMOF deficiency results in embryonic lethality and cell death.

For example, when crossed to B6.129-Tg(Pcp2-cre)2Mpin/J mice (Stock No. 004146) expressing Cre recombinase in Purkinje cells, mMof-deficient mice display impaired motor coordination, ataxia, a backward-walking phenotype, and a reduced life span.

Development
A targeting vector was designed to insert a frt-flanked neomycin resistance (neo) cassette with a 5' loxP site upstream of exon 1, and a single loxP site downstream of exon 3 of the K(lysine) acetyltransferase 8 (Kat8) gene. The construct was electroporated into 129S1/Sv-Oca2+ Tyr+ Kitl+-derived W9.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts and resulting chimeric males were bred with C57BL/6J females. These mMof mice were maintained on a mixed background. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Control Information

  Control
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cell Biology Research
Genes Regulating Growth and Proliferation

Developmental Biology Research
Internal/Organ Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Kat8tm1Thl
Allele Name targeted mutation 1, Thomas Ludwig
Allele Type Targeted (Floxed/Frt)
Common Name(s) Mofflox; mMofflox;
Strain of Origin129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
Gene Symbol and Name Kat8, K(lysine) acetyltransferase 8
Chromosome 7
Gene Common Name(s) 2010203C02Rik; 5830450F21Rik; D7Ertd629e; DNA segment, Chr 7, ERATO Doi 629, expressed; MOF; MYST histone acetyltransferase 1; MYST1; Myst1; RIKEN cDNA 2010203C02 gene; RIKEN cDNA 5830450F21 gene; ZC2HC8; hMOF;
Molecular Note An frt-flanked neo cassette with a 5' loxP site was inserted upstream of intron 1 and an additional loxP site was inserted downstream of exon 3. [MGI Ref ID J:128936]

Genotyping

Genotyping Information

Genotyping Protocols

Generic Neo Melt Curve Analysis, Melt Curve Analysis
Generic Neo Quantitative PCR-QPCR- 1.2, QPCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Gupta A; Guerin-Peyrou TG; Sharma GG; Park C; Agarwal M; Ganju RK; Pandita S; Choi K; Sukumar S; Pandita RK; Ludwig T; Pandita TK. 2008. The mammalian ortholog of Drosophila MOF that acetylates histone H4 lysine 16 is essential for embryogenesis and oncogenesis. Mol Cell Biol 28(1):397-409. [PubMed: 17967868]  [MGI Ref ID J:128936]

Additional References

Kat8tm1Thl related

Kumar R; Hunt CR; Gupta A; Nannepaga S; Pandita RK; Shay JW; Bachoo R; Ludwig T; Burns DK; Pandita TK. 2011. Purkinje cell-specific males absent on the first (mMof) gene deletion results in an ataxia-telangiectasia-like neurological phenotype and backward walking in mice. Proc Natl Acad Sci U S A 108(9):3636-41. [PubMed: 21321203]  [MGI Ref ID J:170483]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, mice homozygous for the floxed allele may be bred together.
Mating SystemHeterozygote x Heterozygote         (Female x Male)   10-JUL-13
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


 

This strain is currently On Hold.
Register Interest

To request more information use the Customer Support Contact Form or call 1-800-422-6423 or 1-207-288-5845

View All Strains Awaiting Transfer from the Donor, In Progress and On Hold

Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHeterozygous for Kat8tm1Thl  
$232.00Female or MaleHomozygous for Kat8tm1Thl  
Price per Pair (US dollars $)Pair Genotype
$302.00Heterozygous for Kat8tm1Thl x Heterozygous for Kat8tm1Thl  

Standard Supply

This strain is currently on HOLD - Contact Customer Service for more information.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHeterozygous for Kat8tm1Thl  
$301.60Female or MaleHomozygous for Kat8tm1Thl  
Price per Pair (US dollars $)Pair Genotype
$392.60Heterozygous for Kat8tm1Thl x Heterozygous for Kat8tm1Thl  

Standard Supply

This strain is currently on HOLD - Contact Customer Service for more information.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

This strain is currently on HOLD - Contact Customer Service for more information.

General Supply Notes

  • 4/14/14: This strain is currently on hold for approximately 10 months. We do not have a working allele specific protocol to distinguish carriers from noncarriers. No open orders will be filled during this time.

Control Information

  Control
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
- Use of MICE by companies or for-profit entities requires a license.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.5)