|These mMof floxed mutant mice possess loxP sites flanking exons 1-3 of the K(lysine) acetyltransferase 8 (Kat8) gene. This strain may be useful for studying the role of histone H4 acetylation of lysine 16 on embryogenesis and organogenesis.|
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Tej Pandita, UTSouthwestern Medical Center
These conditional mMof mice possess loxP sites flanking exons 1-3 of the K(lysine) acetyltransferase 8 (Kat8) gene. Kat8, also called mMOF (murine, males absent on the first), encodes a histone H4 lysine 16 (H4K16)-specific acetyltransferase. Acetylation of H4K16 is required for cellular growth and proliferation during embryogenesis and organogenesis. Overexpression of MOF results in increased H4K16ac levels, increased cellular proliferation, oncogenic transformation, and tumor growth. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 1-3 deleted in cre-expressing tissues. Complete mMOF deficiency results in embryonic lethality and cell death.
For example, when crossed to B6.129-Tg(Pcp2-cre)2Mpin/J mice (Stock No. 004146) expressing Cre recombinase in Purkinje cells, mMof-deficient mice display impaired motor coordination, ataxia, a backward-walking phenotype, and a reduced life span.
A targeting vector was designed to insert a frt-flanked neomycin resistance (neo) cassette with a 5' loxP site upstream of exon 1, and a single loxP site downstream of exon 3 of the K(lysine) acetyltransferase 8 (Kat8) gene. The construct was electroporated into 129S1/Sv-Oca2+ Tyr+ Kitl+-derived W9.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts and resulting chimeric males were bred with C57BL/6J females. These mMof mice were maintained on a mixed background. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
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|Allele Name||targeted mutation 1, Thomas Ludwig|
|Allele Type||Targeted (Floxed/Frt)|
|Common Name(s)||Mofflox; mMofflox;|
|Strain of Origin||129S1/Sv-Oca2<+> Tyr<+> Kitl<+>|
|Gene Symbol and Name||Kat8, K(lysine) acetyltransferase 8|
|Gene Common Name(s)||2010203C02Rik; 5830450F21Rik; D7Ertd629e; DNA segment, Chr 7, ERATO Doi 629, expressed; MOF; MYST histone acetyltransferase 1; MYST1; Myst1; RIKEN cDNA 2010203C02 gene; RIKEN cDNA 5830450F21 gene; ZC2HC8; hMOF;|
|Molecular Note||An frt-flanked neo cassette with a 5' loxP site was inserted upstream of intron 1 and an additional loxP site was inserted downstream of exon 3. [MGI Ref ID J:128936]|
Gupta A; Guerin-Peyrou TG; Sharma GG; Park C; Agarwal M; Ganju RK; Pandita S; Choi K; Sukumar S; Pandita RK; Ludwig T; Pandita TK. 2008. The mammalian ortholog of Drosophila MOF that acetylates histone H4 lysine 16 is essential for embryogenesis and oncogenesis. Mol Cell Biol 28(1):397-409. [PubMed: 17967868] [MGI Ref ID J:128936]
Kumar R; Hunt CR; Gupta A; Nannepaga S; Pandita RK; Shay JW; Bachoo R; Ludwig T; Burns DK; Pandita TK. 2011. Purkinje cell-specific males absent on the first (mMof) gene deletion results in an ataxia-telangiectasia-like neurological phenotype and backward walking in mice. Proc Natl Acad Sci U S A 108(9):3636-41. [PubMed: 21321203] [MGI Ref ID J:170483]
Breeding & Husbandry When maintaining a live colony, mice homozygous for the floxed allele may be bred together.
This strain is currently Awaiting Transfer from the Donor.This strain was accepted into the JAX® Repository on 13-FEB-13. View All Strains Awaiting Transfer from the Donor, In Progress and On Hold
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|Control Pricing Information for Genetically Engineered Mutant Strains.|
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