Strain Name:

B6.129X1-Tasp1tm1.1Jjdh/J

Stock Number:

021298

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Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
These Taspase1-/- mice may be useful for studying TASP1 and MLL gene regulation during embryogenesis.

Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Additional information on Congenic nomenclature.
Mating SystemWild-type x Heterozygote         (Female x Male)   06-SEP-13
Mating SystemHeterozygote x Wild-type         (Female x Male)   06-SEP-13
Specieslaboratory mouse
GenerationN6+pN1F1 (30-DEC-13)
Generation Definitions
 
Donating Investigator James J. Hsieh,   Memorial Sloan-Kettering Cancer Center

Description
These Taspase1-/- mice lack exon 9 of the taspase 1, threonine aspartase 1 (Tasp1) gene, abolishing gene function. Taspase1 is an endopeptidase that cleaves mixed-lineage leukemia (MLL) for proper Homeobox (Hox) gene expression. MLL is a methyltransferase required for sustaining the expression of genes early in embryogenesis. Taspase1 is also required for craniofacial development, fetal hematopoiesis, and has been found overexpressed in some cancer cell lines. Homozygotes are runted and are born at 89% of the expected Mendelian ratio with only 13% surviving to 3 weeks of age due to a feeding defect. They exhibit homeotic transformations in axial skeleton, abnormal craniofacial development, and abnormal outgrowth of cranial nerves. They also show a reduction in hematopoietic stem cell populations and thymocytes. Embryonic fibroblasts (MEFs) exhibit impaired proliferation. Heterozygous Taspase1+/- mice display an intermediate skeletal phenotype.

Development
A targeting vector was designed to insert a single loxP site upstream of exon 9, and a loxP-flanked neomycin resistance (neo) cassette downstream of exon 9 of the taspase, threonine aspartase 1 (Tasp1) gene. The construct was electroporated into 129X1/SvJ-derived RW4 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a CMV-Cre expression plasmid. Resulting ES cells contained multiple gene rearrangments; intact floxed-exon 9, intact floxed-neo cassette, or excision of both exon 9 and the neo cassette. Correctly targeted ES cells, lacking both exon 9 and the neo, were injected into C57BL/6 blastocysts and resulting chimeric males were bred with C57BL/6 females. Resulting offspring were bred to C57BL/6 mice for at least 6 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Tasp1tm1.1Jjdh/Tasp1tm1.1Jjdh

        B6.129X1-Tasp1tm1.1Jjdh
  • nervous system phenotype
  • glossopharyngeal nerve hypoplasia   (MGI Ref ID J:201612)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Tasp1tm1.1Jjdh/Tasp1tm1.1Jjdh

        involves: 129X1/SvJ
  • mortality/aging
  • partial neonatal lethality
    • most die during day 1 or day 2 postnatally   (MGI Ref ID J:112159)
    • only about 13% survive to weaning   (MGI Ref ID J:112159)
  • partial prenatal lethality
    • only about 89% of expected homozygotes are born   (MGI Ref ID J:112159)
  • behavior/neurological phenotype
  • abnormal suckling behavior
    • no indication that pups dying within the first 2 days of life ever suckle   (MGI Ref ID J:112159)
  • growth/size/body phenotype
  • decreased body size
    • markedly smaller than normal immediately after birth   (MGI Ref ID J:112159)
  • decreased embryo size
    • first noticeable at E14.5   (MGI Ref ID J:112159)
    • significantly smaller than normal at E18.5   (MGI Ref ID J:112159)
  • skeleton phenotype
  • abnormal sternebra morphology
    • incomplete segmentation between sternebrae 3 and 4   (MGI Ref ID J:112159)
  • abnormal vertebrae morphology   (MGI Ref ID J:112159)
    • abnormal cervical vertebrae morphology   (MGI Ref ID J:112159)
      • abnormal arcus anterior morphology
        • anterior arch of the atlas is broadened and tilted anteriorly   (MGI Ref ID J:112159)
      • abnormal cervical axis morphology
        • split neural arch of the axis   (MGI Ref ID J:112159)
      • cervical vertebral transformation
        • posterior transformation of C7 to T1 with attached aberrant ribs   (MGI Ref ID J:112159)
    • vertebral transformation   (MGI Ref ID J:112159)
      • cervical vertebral transformation
        • posterior transformation of C7 to T1 with attached aberrant ribs   (MGI Ref ID J:112159)
      • lumbar vertebral transformation
        • anterior transformation of L1 to T13   (MGI Ref ID J:112159)
      • thoracic vertebral transformation
        • anterior transformation of T8 to T7 with resulting additional sternal ribs   (MGI Ref ID J:112159)
  • increased rib number
    • 14 pairs of ribs; additional pair of sternal ribs   (MGI Ref ID J:112159)
  • cellular phenotype
  • decreased cell proliferation
    • delayed entry of embryonic fibroblasts into the S phase of the cell cycle   (MGI Ref ID J:112159)
  • embryogenesis phenotype
  • decreased embryo size
    • first noticeable at E14.5   (MGI Ref ID J:112159)
    • significantly smaller than normal at E18.5   (MGI Ref ID J:112159)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cancer Research
Genes Regulating Growth and Proliferation

Cell Biology Research
Cell Cycle Regulation
Genes Regulating Growth and Proliferation

Developmental Biology Research
Craniofacial and Palate Defects
Internal/Organ Defects
      hematopoietic defects
Lymphoid Tissue Defects
      hematopoietic defects
Postnatal Lethality
      Homozygous
Skeletal Defects

Internal/Organ Research
Skeleton
      Bone

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tasp1tm1.1Jjdh
Allele Name targeted mutation 1.1, James JD Hsieh
Allele Type Targeted (knock-out)
Common Name(s) Tasp1-;
Strain of Origin129X1/SvJ
Gene Symbol and Name Tasp1, taspase, threonine aspartase 1
Chromosome 2
Gene Common Name(s) 4930485D02Rik; AW986064; C20orf13; RGD1308591; RIKEN cDNA 4930485D02 gene; dJ585I14.2; expressed sequence AW986064;
Molecular Note A loxP site was inserted upstream of exon 9 and a floxed PGK neo cassette was inserted into intron 9. Successfully targeted ES cells were subsequently transiently transfected with CMV-cre to excise both exon9 9 and the PGKneo. Protein was not detected in mutant MEFs. [MGI Ref ID J:112159]

Genotyping

Genotyping Information

Genotyping Protocols

Tasp1tm1.1Jjdh, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Takeda S; Chen DY; Westergard TD; Fisher JK; Rubens JA; Sasagawa S; Kan JT; Korsmeyer SJ; Cheng EH; Hsieh JJ. 2006. Proteolysis of MLL family proteins is essential for Taspase1-orchestrated cell cycle progression. Genes Dev 20(17):2397-409. [PubMed: 16951254]  [MGI Ref ID J:112159]

Additional References

Tasp1tm1.1Jjdh related

Chen DY; Lee Y; Van Tine BA; Searleman AC; Westergard TD; Liu H; Tu HC; Takeda S; Dong Y; Piwnica-Worms DR; Oh KJ; Korsmeyer SJ; Hermone A; Gussio R; Shoemaker RH; Cheng EH; Hsieh JJ. 2012. A pharmacologic inhibitor of the protease Taspase1 effectively inhibits breast and brain tumor growth. Cancer Res 72(3):736-46. [PubMed: 22166309]  [MGI Ref ID J:181107]

Takeda S; Liu H; Sasagawa S; Dong Y; Trainor PA; Cheng EH; Hsieh JJ. 2013. HGF-MET signals via the MLL-ETS2 complex in hepatocellular carcinoma. J Clin Invest 123(7):3154-65. [PubMed: 23934123]  [MGI Ref ID J:201612]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           FGB27

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony or to C57BL/6J inbred mice (Stock No. 000664).
Mating SystemWild-type x Heterozygote         (Female x Male)   06-SEP-13
Heterozygote x Wild-type         (Female x Male)   06-SEP-13
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHeterozygous for Tasp1tm1.1Jjdh  
Price per Pair (US dollars $)Pair Genotype
$302.00Heterozygous for Tasp1tm1.1Jjdh x Wild-type for Tasp1tm1.1Jjdh  
$302.00Wild-type for Tasp1tm1.1Jjdh x Heterozygous for Tasp1tm1.1Jjdh  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHeterozygous for Tasp1tm1.1Jjdh  
Price per Pair (US dollars $)Pair Genotype
$392.60Heterozygous for Tasp1tm1.1Jjdh x Wild-type for Tasp1tm1.1Jjdh  
$392.60Wild-type for Tasp1tm1.1Jjdh x Heterozygous for Tasp1tm1.1Jjdh  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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