Strain Name:

STOCK Iis5tm2.1(ACTB-EGFP,-tdTomato)Luo/J

Stock Number:

021457

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Availability:

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Use Restrictions Apply, see Terms of Use
The MADM-7GT (Hipp7GT) allele has the CMV enhancer/chicken beta-actin core promoter, the N-terminal portion of mut4-EGFP, a beta-globin intronic sequence (containing frt and lox sites), and the MYC-tagged C-terminal portion of tdTomato all inserted into the Hipp7 locus on chromosome 7 (~2.13 cM; ~0.7 kbp downstream of exon 5 of the Rps9 gene). These MADM-7GT mutants are designed for MADM (mosaic analysis with double markers), and must be crossed to MADM-7TG mice harboring a reciprocal mutation at the same locus (Stock No. 021458). This MADM system allows Cre recombinase-induced fluorescent labeling of daughter cells to ascertain lineal relationships and pleiotropic gene function in multicellular organisms. These mice may also be useful in studies of cell differentiation, mitosis, and imprinting.

Description

Strain Information

Former Names STOCK Iis5tm2(ACTB-EGFP,-tdTomato)Luo/J    (Changed: 03-JUL-13 )
Type Mutant Stock; Targeted Mutation;
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Mating SystemHomozygote x Homozygote         (Female x Male)   25-FEB-14
Specieslaboratory mouse
Generation?+pN1 (08-OCT-13)
Generation Definitions
 
Donating InvestigatorDr. Liqun Luo,   Stanford University, HHMI
Donating Investigator Simon Hippenmeyer,   IST Austria (Institute of Science and Technology Austria)

Description
Mice homozygous for the MADM-7GT (Hipp7GT) allele are viable and fertile with no reported abnormalities. The MADM-7GT allele has the CMV enhancer/chicken beta-actin core promoter, the N-terminal portion of mut4-EGFP, a beta-globin intronic sequence (containing a frt-flanked region of two lox site variants [lox2272 and lox5171] and then a single loxP site), and the MYC-tagged C-terminal portion of tdTomato all inserted into the Hipp7 locus on chromosome 7 (~2.13 cM; ~0.7 kbp downstream of exon 5 of the Rps9 gene). These MADM-7GT mutants are designed for MADM (mosaic analysis with double markers), and must be crossed to MADM-7TG mice harboring a reciprocal mutation at the same locus (Stock No. 021458). The resulting GT/TG offspring have one copy of each reciprocal mutation on homologous chromosomes ("trans-heterozygous"), and must also be bred to harbor a Cre- or FLP-recombinase to induce fluorescent protein expression. Prior to Cre- or FLP-recombination, trans-heterozygous mutant mice do not have colored cells: the chimeric genes do not produce functional proteins because their coding sequences are interrupted by the beta-globin intron in different reading frames. After DNA replication (G2 phase) in double mutant mice, introduction of Cre- or FLP-recombinase that facilitates inter-chromosomal recombination aligns the respective N- and C-terminal coding sequences for each of the reporter genes on the same chromosome. The subsequent chromatid segregation (X or Z) determines daughter cell phenotype: recombinant sister chromatids into the same daughter cell (a G2-Z event) leads to double reporter expression or no reporter expression, while independent segregation into separate daughter cells (a G2-X event) leads to expression of either EGFP or tdTomato-MYC. If an additional targeted mutation of interest is introduced distal to the Hipp7 locus on chromosome 7, only homozygous cells will be singly labeled following G2 cre or FLP introduction. The homozygous mutant and wildtype cells can then be distinguished by which single reporter they express. Most heterozygous cells will be unlabeled, but some heterozygous cells will be yellow (both markers expressed). Reporter protein tissue specificity, expression levels, and frequency of recombination are thus determined by the promoter controlling Cre- or FLP-recombinase expression. Using this MADM system, a researcher can generate genetic mosaics in which an individual organism contains somatic cells of different genotypes. This allows the researcher to ascertain lineal relationships and pleiotropic gene function in multicellular organisms. These mice may also be useful in studies of cell differentiation and mitosis.

Other important features of the MADM-7 system are listed below. Because of its placement ~3.6 Mbp from the centromere, MADM-7 allows almost all of the genes on chromosome 7 to be subjected to MADM-based mosaic analyses. Cre- or FLP-recombinase introduction in cell phase G0 or G1 results in double reporter expression. The donating investigator also reports the MADM-7 design has several advantages compared to the original MADM(-6) mice. Specifically, MADM-7 allows direct fluorescent visualization of both EGFP and tdTomato in live animals/cells: permitting genotypes of distinctly labeled cells in mosaic animals to be unequivocally determined prior to fixation and/or immunostaining. Also, MADM-7 contains both loxP and frt sites; allowing the induction of MADM-labeling by either Cre recombinase or FLP recombinase.

The same donating investigator has several mice with MADM applications on different chromosomes:
On chromosome 6, the Gt(ROSA)26Sor knockin mutations include MADM-6GR (Stock Nos. 006041 / 006075), MADM-6RG (Stock Nos. 006067 / 006080), MADM-6GG (Stock Nos. 006053 / 006071), R26GT (Stock No. 017912), R26TG (Stock No. 017921), R26TT (Stock No. 017922), and R26G-tTA2 (Stock No. 017909).
On chromosome 7, the centromeric insertions are MADM-7GT (Hipp7GT; Stock No. 021457) and MADM-7TG (Hipp7TG; Stock No. 021458).
On chromosome 10, the centromeric insertions are MADM-10GT (Miya10GT; Stock No. 017923) and MADM-10TG (Miya10TG; Stock No. 017932).
On chromosome 11, the centromeric insertions are MADM-11GT (Hipp11GT; Stock No. 013749) and MADM-11TG (Hipp11TG; Stock No. 013751).
On chromosome 12, the centromeric insertions are MADM-12GT (John12GT; Stock No. 021460) and MADM-12TG (John12TG; Stock No. 021461).

Development
A targeting vector was created with a CMV enhancer/chicken beta-actin core promoter (pCA) upstream of the "GT MADM" cassette. The "GT MADM" cassette used here was designed with the N-terminal portion of a mutant enhanced green fluorescent protein (mut4-EGFP; aa 1-273), a beta-globin intronic sequence (containing a frt-flanked region of two lox site variants [lox2272 and lox5171] and then a loxP-flanked neomycin resistance gene), the C-terminal portion of a red fluorescent protein (tdTomato; aa 4-1544) tagged with three copies of the Myc epitope at its C-terminus, and an SV40 T-antigen poly(A) signal. This entire "GT MADM" construct was inserted into the Hipp7 locus on chromosome 7 (~2.13 cM; ~0.7 kbp downstream of exon 5 of the Rps9 gene) via electroporation of (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were microinjected into recipient blastocysts. Chimeric males were bred to CD1 females to establish the MADM-7GT colony. The donating investigator reports that MADM-7GT mice were previously bred to Hprt-Cre mice (on an undisclosed genetic background; see Stock No. 004302), and the floxed neomycin resistance gene was removed. The donating investigator also reports that the MADM-7GT mice no longer have the Cre recombinase gene. MADM-7GT mice on a mixed CD1;129;C57BL/6 genetic background (white coat color) were sent to The Jackson Laboratory Repository in 2013. Upon arrival, sperm was cryopreserved. To generate the living colony, an aliquot of the frozen sperm was used to fertilize oocytes from C57BL/6J female mice (Stock No. 000664). The MADM-7GT mice were then bred together, to wildtype mice from the colony, or to C57BL/6J mice.

Control Information

  Control
   101045 B6129SF2/J (approximate)
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls

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006043   B6;SJL-Tg(Oxt/EGFP)AI03Wsy/J
012355   B6;SJL-Tg(Pvalb-COP4*H134R/EYFP)15Gfng/J
012341   B6;SJL-Tg(Thy1-COP3/EYFP)1Gfng/J
012344   B6;SJL-Tg(Thy1-COP3/EYFP)4Gfng/J
012348   B6;SJL-Tg(Thy1-COP3/EYFP)8Gfng/J
012350   B6;SJL-Tg(Thy1-COP4*H134R/EYFP)20Gfng/J
008004   B6;SJL-Tg(Thy1-ECFP/VAMP2)1Sud/J
007610   B6;SJL-Tg(Thy1-cre/ERT2,-EYFP)VGfng/J
012332   B6;SJL-Tg(Thy1-hop/EYFP)2Gfng/J
012334   B6;SJL-Tg(Thy1-hop/EYFP)4Gfng/J
014555   B6;SJL-Tg(Tph2-COP4*H134R/EYFP)5Gfng/J
019013   B6N.129S6(Cg)-Gt(ROSA)26Sortm2(EGFP/cre)Alj/J
023537   B6N.129S6-Gt(ROSA)26Sortm1(CAG-tdTomato*,-EGFP*)Ees/J
018974   B6N.B6-Tg(Nr4a1-EGFP/cre)820Khog/J
018913   B6N.Cg-Tg(tetO-GFP,-lacZ)G3Rsp/J
016532   B6N.FVB(Cg)-Tg(CAG-rtTA3)4288Slowe/J
007880   B6SJL-Tg(Thy1-Stx1a/EYFP)1Sud/J
007856   B6SJL-Tg(Thy1-Syt1/ECFP)1Sud/J
004190   C.129-Il4tm1Lky/J
005700   C.129P2-Cxcr6tm1Litt/J
017580   C.129S4(B6)-Ifngtm3.1Lky/J
015864   C.129S4(B6)-Il12btm1Lky/J
017353   C.129S4(B6)-Il13tm1(YFP/cre)Lky/J
006769   C.Cg-Foxp3tm2Tch/J
010545   C.FVB-Tg(CAG-luc,-GFP)L2G85Chco/FathJ
004512   C.FVB-Tg(Itgax-DTR/EGFP)57Lan/J
008591   C57BL/6-Ackr3tm1Litt/J
008374   C57BL/6-Foxp3tm1Flv/J
008517   C57BL/6-Gt(ROSA)26Sortm3(CAG-MIR17-92,-EGFP)Rsky/J
012343   C57BL/6-Gt(ROSA)26Sortm7(Pik3ca*,EGFP)Rsky/J
012352   C57BL/6-Gt(ROSA)26Sortm8(Map2k1*,EGFP)Rsky/J
012361   C57BL/6-Gt(ROSA)26Sortm9(Rac1*,EGFP)Rsky/J
010724   C57BL/6-Trim21tm1Hm/J
006567   C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ
003291   C57BL/6-Tg(CAG-EGFP)1Osb/J
005070   C57BL/6-Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck/J
012943   C57BL/6-Tg(Ins2-luc/EGFP/TK)300Kauf/J
016617   C57BL/6-Tg(Nr4a1-EGFP/cre)820Khog/J
012890   C57BL/6-Tg(Scgb1a1-Il17f,GFP)1Cdon/J
004353   C57BL/6-Tg(UBC-GFP)30Scha/J
005706   C57BL/6-Tg(tetO-CDK5R1/GFP)337Lht/J
006618   C57BL/6-Tg(tetO-COX8A/EYFP)1Ksn/J
006362   C57BL/6J-Tg(CMV-Cox8a/EYFP)17J/J
009655   C57BL/6J-Tg(Dcx-DsRed)14Qlu/J
007857   C57BL/6J-Tg(Eno2-YFP/Cox8a)YRwb/J
007860   C57BL/6J-Tg(Eno2-YFP/Cox8a)ZRwb/J
007567   C57BL/6J-Tg(Itgax-cre,-EGFP)4097Ach/J
009593   C57BL/6J-Tg(Pomc-EGFP)1Low/J
003927   C57BL/6J-Tg(Sry-EGFP)92Ei/EiJ
008234   CB6-Tg(CAG-EGFP/CETN2)3-4Jgg/J
007677   CB6-Tg(Gad1-EGFP)G42Zjh/J
007898   CBy.Cg-Tg(Gt(ROSA)26Sor-EGFP)I1Able/J
007075   CByJ.B6-Tg(CAG-EGFP)1Osb/J
007076   CByJ.B6-Tg(UBC-GFP)30Scha/J
010548   D1.FVB(Cg)-Tg(CAG-luc,-GFP)L2G85Chco/FathJ
008450   FVB-Tg(CAG-luc,-GFP)L2G85Chco/J
003718   FVB-Tg(GadGFP)45704Swn/J
010947   FVB-Tg(Gstm5-EGFP)1Ilis/J
005515   FVB-Tg(ITGAM-DTR/EGFP)34Lan/J
010588   FVB-Tg(Myh6/NFAT-luc)1Jmol/J
006421   FVB-Tg(Pomc1-hrGFP)1Lowl/J
005688   FVB-Tg(Rag2-EGFP)1Mnz/J
005125   FVB.129S6(B6)-Gt(ROSA)26Sortm1(Luc)Kael/J
006206   FVB.129S6-Gt(ROSA)26Sortm2(HIF1A/luc)Kael/J
012429   FVB.Cg-Gt(ROSA)26Sortm1(CAG-lacZ,-EGFP)Glh/J
003516   FVB.Cg-Tg(CAG-EGFP)B5Nagy/J
016573   FVB.Cg-Tg(SMN2)89Ahmb Smn1tm1Msd Tg(S100B-EGFP)1Wjt Tg(SMN2*delta7)4299Ahmb/J
007483   FVB.Cg-Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ
008200   FVB/N-Tg(CAG-EGFP,-ALPP)2.6Ggc/J
009354   FVB/N-Tg(Dazl-EGFP)10Rarp/J
003257   FVB/N-Tg(GFAPGFP)14Mes/J
007800   FVB/N-Tg(Ins1-luc)VUPwrs/J
012370   FVB/NJ-Tg(Hspa1a-luc,-EGFP)2Chco/J
009618   NOD.129(B6)-Il12btm1Lky/JbsJ
013116   NOD.B6-Tg(Ins2-luc/EGFP/TK)300Kauf/J
013233   NOD.B6-Tg(Itgax-cre,-EGFP)4097Ach/J
006698   NOD.Cg-Il4tm1Lky/JbsJ
008173   NOD.Cg-Tg(Ins1-EGFP)1Hara/QtngJ
009422   NOD.Cg-Tg(Itgax-Venus)1Mnz/QtngJ
005076   NOD.Cg-Tg(tetO-EGFP/FADD)1Doi/DoiJ
010542   NOD.FVB-Tg(CAG-luc,-GFP)L2G85Chco/FathJ
008547   NOD.FVB-Tg(ITGAM-DTR/EGFP)34Lan/JdkJ
008549   NOD.FVB-Tg(Itgax-DTR/EGFP)57Lan/JdkJ
005082   NOD/ShiLt-Tg(ACTB-Ica1/EGFP)18Mdos/MdosJ
005328   NOD/ShiLt-Tg(Cd4-DsRed)4Lt/J
005334   NOD/ShiLt-Tg(Cd4-EGFP)1Lt/J
008694   NOD/ShiLt-Tg(Foxp3-EGFP/cre)1cJbs/J
005282   NOD/ShiLtJ-Tg(Ins1-EGFP/GH1)14Hara/HaraJ
012881   STOCK Ascl1tm1Reed/J
008666   STOCK Fmn1tm1Made/J
013731   STOCK Gt(ROSA)26Sortm1(CAG-Brainbow2.1)Cle/J
006331   STOCK Gt(ROSA)26Sortm1(DTA)Jpmb/J
005130   STOCK Gt(ROSA)26Sortm1(Smo/EYFP)Amc/J
005572   STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
017922   STOCK Gt(ROSA)26Sortm10(ACTB-tdTomato)Luo/J
018903   STOCK Gt(ROSA)26Sortm2(EGFP/cre)Alj/J
007576   STOCK Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J
017912   STOCK Gt(ROSA)26Sortm6(ACTB-EGFP*,-tdTomato)Luo/J
017921   STOCK Gt(ROSA)26Sortm7(ACTB-EGFP*)Luo/J
017909   STOCK Gt(ROSA)26Sortm8(ACTB-EGFP*,-tTA2)Luo/J
008876   STOCK Hprttm11(Ple176-EGFP/cre)Ems/Mmjax
009349   STOCK Hprttm31(Ple67-EGFP)Ems/Mmjax
009594   STOCK Hprttm32(Ple112-EGFP)Ems/Mmjax
022976   STOCK Igs2tm1(ACTB-EGFP,-tdTomato)Zng/J
022977   STOCK Igs2tm2(ACTB-tdTomato,-EGFP)Zng/J
013749   STOCK Iis2tm1(ACTB-EGFP,-tdTomato)Luo/J
013751   STOCK Iis2tm2(ACTB-tdTomato,-EGFP)Luo/J
017932   STOCK Iis3tm1.1(ACTB-EGFP*)Luo/J
017923   STOCK Iis3tm2.1(ACTB-EGFP*,-tdTomato)Luo/J
021458   STOCK Iis5tm1(ACTB-tdTomato,-EGFP)Luo/J
021461   STOCK Iis6tm1.1(ACTB-tdTomato,-EFGP)Luo/J
021460   STOCK Iis6tm2.1(ACTB-EFGP,-tdTomato)Luo/J
004808   STOCK Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J
004779   STOCK Mapttm1(EGFP)Klt/J
005692   STOCK Nphs1tm1Rkl/J
006741   STOCK Olfr160tm1(Olfr151)Mom Tg(Olfr151,taulacZ)BMom/MomJ
006678   STOCK Olfr160tm6Mom/MomJ
006669   STOCK Olfr17tm7Mom/MomJ
009061   STOCK Osr1tm1(EGFP/cre/ERT2)Amc/J
007879   STOCK Stx1atm2Sud/J
014581   STOCK Trpm8tm1Apat/J
010911   STOCK Wt1tm1(EGFP/cre)Wtp/J
005438   STOCK Tg(CAG-Bgeo,-DsRed*MST)1Nagy/J
006850   STOCK Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe/J
006876   STOCK Tg(CAG-Bgeo,-TEL/AML1,-EGFP)A6Lbe/J
003920   STOCK Tg(CAG-Bgeo/GFP)21Lbe/J
005441   STOCK Tg(CAG-DsRed*MST)1Nagy/J
003773   STOCK Tg(CAG-ECFP)CK6Nagy/J
003115   STOCK Tg(CAG-EGFP)B5Nagy/J
003116   STOCK Tg(CAG-EGFP)D4Nagy/J
011106   STOCK Tg(CAG-GFP*)1Hadj/J
013754   STOCK Tg(CAG-KikGR)75Hadj/J
011107   STOCK Tg(CAG-Venus)1Hadj/J
005645   STOCK Tg(CAG-mRFP1)1F1Hadj/J
005105   STOCK Tg(Chx10-EGFP/cre,-ALPP)2Clc/J
018322   STOCK Tg(Cp-EGFP)25Gaia/ReyaJ
008241   STOCK Tg(Cspg4-DsRed.T1)1Akik/J
006334   STOCK Tg(Gad1-EGFP)94Agmo/J
006340   STOCK Tg(Gad1-EGFP)98Agmo/J
007896   STOCK Tg(Gt(ROSA)26Sor-EGFP)I1Able/J
016252   STOCK Tg(Hoxb7-Venus*)17Cos/J
006784   STOCK Tg(Ins1-Cerulean)24Hara/J
006866   STOCK Tg(Ins1-DsRed*T4)32Hara/J
016921   STOCK Tg(Myh2-DsRed2)1Jrs/J
012477   STOCK Tg(Myh6*/tetO-GCaMP2)1Mik/J
016922   STOCK Tg(Myh7-CFP)1Jrs/J
008579   STOCK Tg(PSCA-EGFP)1Witt/J
012452   STOCK Tg(Rr5-GFP/cre)1Sapc/J
006570   STOCK Tg(SMN2)89Ahmb Smn1tm1Msd Tg(Hlxb9-GFP)1Tmj/J
009606   STOCK Tg(Six2-EGFP/cre)1Amc/J
003658   STOCK Tg(TIE2GFP)287Sato/J
013162   STOCK Tg(Thy1-Clomeleon)12Gjau/J
013163   STOCK Tg(Thy1-Clomeleon)13Gjau/J
007788   STOCK Tg(Thy1-EGFP)MJrs/J
012708   STOCK Tg(Thy1-cre/ERT2,-EYFP)HGfng/PyngJ
011108   STOCK Tg(Ttr-RFP)1Hadj/J
016981   STOCK Tg(Uchl1-HIST2H2BE/mCherry/EGFP*)FSout/J
006129   STOCK Tg(Zp3-EGFP)1Dean/J
003274   STOCK Tg(tetNZL)2Bjd/J
005104   STOCK Tg(tetO-HIST1H2BJ/GFP)47Efu/J
005699   STOCK Tg(tetO-Ipf1,EGFP)956.6Macd/J
017918   STOCK Tg(tetO-MAML1*/EGFP)2Akar/J
012345   STOCK Tg(tetO-tdTomato,-Syp/EGFP*)1.1Luo/J
View Fluorescent Protein Strains     (375 strains)

Strains carrying other alleles of Igs5
021458   STOCK Iis5tm1(ACTB-tdTomato,-EGFP)Luo/J
View Strains carrying other alleles of Igs5     (1 strain)

Additional Web Information

FLP-FRT Systems

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Igs5tm2.1(ACTB-EGFP,-tdTomato)Luo/Igs5tm2.1(ACTB-EGFP,-tdTomato)Luo

        involves: 129S1/Sv * 129X1/SvJ
  • normal phenotype
  • no abnormal phenotype detected
    • born at Mendelian frequencies   (MGI Ref ID J:194079)
    • have a normal lifespan   (MGI Ref ID J:194079)
    • are fertile   (MGI Ref ID J:194079)
    • no obvious averse phenotype   (MGI Ref ID J:194079)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cell Biology Research
Cell Cycle Regulation
Genes Regulating Growth and Proliferation

Developmental Biology Research
Imprinting

Neurobiology Research
Cre-lox System
      loxP-flanked Sequences
      loxP-flanked Sequences: Test/Reporter
Fluorescent protein expression in neural tissue

Research Tools
Cardiovascular Research
      Cre-lox System
Cell Biology Research
Cre-lox System
      loxP-flanked Sequences
      loxP-flanked Sequences: Test/Reporter
Developmental Biology Research
      Cre-lox System
Diabetes and Obesity Research
      loxP
FLP-FRT System
      FRT-flanked Sequences
      FRT-flanked Sequences: Test/Reporter
Fluorescent Proteins
Genetics Research
      Mutagenesis and Transgenesis
      Mutagenesis and Transgenesis: Cre-lox System
      Tissue/Cell Markers
      Tissue/Cell Markers: Cre-lox System
Neurobiology Research
      cell marker
Reproductive Biology Research
      Cre-lox System

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Igs5tm2.1(ACTB-EGFP,-tdTomato)Luo
Allele Name targeted mutation 2.1, Liqun Luo
Allele Type Targeted (Floxed/Frt)
Common Name(s) Iis5tm2.1(ACTB-EGFP,-tdTomato)Luo; MADM-7GT;
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name Igs5, intergenic site 5
Chromosome 7
Gene Common Name(s) Hipp7; Iis5; MADM-7;
Molecular Note The GT MADM targeting construct was designed with a CMV enhancer/chicken beta-actin core promoter (pCA), the N-terminal portion of a mutant enhanced green fluorescent protein (mut4-EGFP; aa 1-273), a beta-globin intronic sequence containing an frt site and loxP-flanked neomycin resistance gene, the C-terminal portion of a red fluorescent protein (tdTomato; aa 4-1544) tagged with three copies of the Myc epitope at its C-terminus, and an SV40 T-antigen poly(A) signal. This entire GT MADM construct was inserted into the Iis5 locus locate at about 0.7 kb downstream of exon 5 of the Rps9 gene at 2.13cM. Mice were bred to Hprt-Cre mice (on an undisclosed genetic background) and the floxed neomycin resistance gene was removed. [MGI Ref ID J:194079]

Genotyping

Genotyping Information

Genotyping Protocols

Iis (GT vs TG), Separated PCR
Iis5tm2.1(ACTB-EGFP,-tdTomato)Luo,

SEPARATED MELT



Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Hippenmeyer S; Johnson RL; Luo L. 2013. Mosaic Analysis with Double Markers Reveals Cell-Type-Specific Paternal Growth Dominance. Cell Rep :. [PubMed: 23453967]  [MGI Ref ID J:194079]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           FGB27

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice may be bred together.
Mating SystemHomozygote x Homozygote         (Female x Male)   25-FEB-14
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHomozygous for Iis5tm2(ACTB-EGFP,-tdTomato)Luo  
Price per Pair (US dollars $)Pair Genotype
$464.00Homozygous for Iis5tm2(ACTB-EGFP,-tdTomato)Luo x Homozygous for Iis5tm2(ACTB-EGFP,-tdTomato)Luo  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHomozygous for Iis5tm2(ACTB-EGFP,-tdTomato)Luo  
Price per Pair (US dollars $)Pair Genotype
$603.20Homozygous for Iis5tm2(ACTB-EGFP,-tdTomato)Luo x Homozygous for Iis5tm2(ACTB-EGFP,-tdTomato)Luo  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Control Information

  Control
   101045 B6129SF2/J (approximate)
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
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Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
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Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
- Use of MICE by companies or for-profit entities requires a license.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

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In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

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In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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