Strain Name:

STOCK Mir146tm1Mtm/Mmjax

Availability:

Cryopreserved - Ready for recovery     Available at the JAX MMRRC

Please refer to the Mutant Mouse Regional Resource Center (MMRRC) for information about STOCK Mir146tm1Mtm/Mmjax MMRRC Stock Number 036799.
These microRNA 146 conditional mutant mice are designed to generate a null allele or a lacZ tagged null allele when combined with Flp or Cre recombinase expressing strains. This mutant mouse strain may be useful in studies of microRNA biology.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Mating SystemHomozygote x Homozygote         (Female x Male)   04-NOV-13
Specieslaboratory mouse
 
Donating Investigator Mike McManus,   University of California, San Francisco

Description
Mice that are homozygous for the targeted mutation are viable and fertile. The Donating Investigator has not generated homozygous animals. When combined with a Flp recombinase-expressing strain, the lacZ and neomycin genes are removed leaving an FRT site and the loxP-flanked miR146 stem loop. A further cross to a Cre-recombinase-expressing strain generates the null allele. When combined with a Cre-recombinase-expressing strain, the neomycin cassette and miR146 stem loop are removed leaving a lacZ tagged null allele (FRT-lacZ-loxP). LacZ expression is not observed in whole mount stains at E11.5. Differential expression of lacZ has been observed in T cells. This mutant mouse strain may be useful in studies of microRNA biology. Additional information about this mirKO mouse can be found here.

Development
A targeting vector was designed to insert an FRT site followed by a lacZ gene, a loxP site, a neomycin cassette, an FRT site and a loxP site (FRT-lacZ-loxP-Neo-FRT-loxP) upstream of the microRNA stem loop and one loxP site immediately downstream of the microRNA stem loop. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Chimeric mice were crossed with a mouse of mixed genetic background (which incorporated FVB), then crossed to inbred C57BL/6. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.

Control Information

  Control
   None Available
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Mir146
016239   B6(FVB)-Mir146tm1.1Bal/J
View Strains carrying other alleles of Mir146     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Mir146tm1Mtm/Mir146tm1Mtm

        involves: 129P2/OlaHsd * C57BL/6
  • no phenotypic analysis
  • *normal* no phenotypic analysis   (MGI Ref ID J:181831)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cell Biology Research
microRNA
      loxP-flanked microRNA sequence

Neurobiology Research
lacZ expression in neural tissue
Cre-lox System
      loxP-flanked Sequences
      loxP-flanked Sequences: Test/Reporter

Research Tools
lacZ Expression
Cre-lox System
      loxP-flanked Sequences: Test/Reporter
Diabetes and Obesity Research
      loxP

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Mir146tm1Mtm
Allele Name targeted mutation 1, Michael McManus
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Strain of Origin129P2/OlaHsd
Site of ExpressionNo expression at E11.5
Gene Symbol and Name Mir146, microRNA 146
Chromosome 11
Gene Common Name(s) Mirn146; miR-146a; mmu-mir-146; mmu-mir-146a;
Molecular Note vA targeting vector was designed to insert an FRT site followed by a lacZ gene, a loxP site, a neomycin cassette, an FRT site and a loxP site (FRT-lacZ-loxP-Neo-FRT-loxP) upstream of the microRNA stem loop and one loxP site immediately downstream of the microRNA stem loop. [MGI Ref ID J:181752]

Genotyping

Genotyping Information

Genotyping Protocols

Mir146tm1Mtm/Mmjax alternate1, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Park CY; Jeker LT; Carver-Moore K; Oh A; Liu HJ; Cameron R; Richards H; Li Z; Adler D; Yoshinaga Y; Martinez M; Nefadov M; Abbas AK; Weiss A; Lanier LL; de Jong PJ; Bluestone JA; Srivastava D; McManus MT. 2012. A resource for the conditional ablation of microRNAs in the mouse Cell Rep 1(4):385-91. [PubMed: 22570807]  [MGI Ref ID J:181752]

W.M. Keck Center for Noncoding RNAs. 2012. Information obtained from the W.M. Keck Center for Noncoding RNAs Unpublished :.  [MGI Ref ID J:181831]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhile maintaining a live colony, these mice are bred as homozygotes.
Mating SystemHomozygote x Homozygote         (Female x Male)   04-NOV-13

Pricing and Purchasing

Supply Notes


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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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