|Please refer to the Mutant Mouse Regional Resource Center (MMRRC) for information about STOCK Mir325tm1Mtm/Mmjax MMRRC Stock Number 036800.|
|These microRNA 325 conditional mutant mice are designed to generate a null allele or a lacZ tagged null allele when combined with Flp or Cre recombinase expressing strains. lacZ expression is observed in the central nervous system, brain, spinal cord and visual sensory system.. This mutant mouse strain may be useful in studies of microRNA biology.|
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Mike McManus, University of California, San Francisco
Mice that are homozygous for the targeted mutation are viable and fertile. When combined with a Flp recombinase-expressing strain, the lacZ and neomycin genes are removed leaving an FRT site and the loxP-flanked miR325 stem loop. A further cross to a Cre-recombinase-expressing strain generates the null allele. When combined with a Cre-recombinase-expressing strain, the neomycin cassette and miR325 stem loop are removed leaving a lacZ tagged null allele (FRT-lacZ-loxP). LacZ expression is observed in the central nervous system, brain, spinal cord and visual sensory system. This mutant mouse strain may be useful in studies of microRNA biology. Additional information about this mirKO mouse can be found here.
A targeting vector was designed to insert an FRT site followed by a lacZ gene, a loxP site, a neomycin cassette, an FRT site and a loxP site (FRT-lacZ-loxP-Neo-FRT-loxP) upstream of the microRNA stem loop and one loxP site immediately downstream of the microRNA stem loop. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Chimeric mice were crossed with a mouse of mixed genetic background (which incorporated FVB), then crossed to inbred C57BL/6. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
|Wild-type from the colony|
|Considerations for Choosing Controls|
View Mammalian Phenotype TermsMammalian Phenotype Terms provided by MGIassigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Mir325tm1Mtm/Mir325tm1Mtminvolves: 129P2/OlaHsd * C57BL/6
- no phenotypic analysis
- *normal* no phenotypic analysis (MGI Ref ID J:181831)
View Research ApplicationsResearch ApplicationsThis mouse can be used to support research in many areas including:
Cell Biology Research
loxP-flanked microRNA sequence
lacZ expression in neural tissue
loxP-flanked Sequences: Test/Reporter
loxP-flanked Sequences: Test/Reporter
|Allele Name||targeted mutation 1, Michael McManus|
|Allele Type||Targeted (Floxed/Frt)|
|Strain of Origin||129P2/OlaHsd|
|Gene Symbol and Name||Mir325, microRNA 325|
|Gene Common Name(s)||Mirn325; mir 325; mmu-mir-325;|
|Molecular Note||A targeting vector was designed to insert an FRT site followed by a lacZ gene, a loxP site, a neomycin cassette, an FRT site and a loxP site (FRT-lacZ-loxP-Neo-FRT-loxP) upstream of the microRNA stem loop and one loxP site immediately downstream of the microRNA stem loop. [MGI Ref ID J:181752]|
Park CY; Jeker LT; Carver-Moore K; Oh A; Liu HJ; Cameron R; Richards H; Li Z; Adler D; Yoshinaga Y; Martinez M; Nefadov M; Abbas AK; Weiss A; Lanier LL; de Jong PJ; Bluestone JA; Srivastava D; McManus MT. 2012. A resource for the conditional ablation of microRNAs in the mouse Cell Reports 1(4):385-91. [PubMed: 22570807] [MGI Ref ID J:181752]
W.M. Keck Center for Noncoding RNAs. 2012. Information obtained from the W.M. Keck Center for Noncoding RNAs Unpublished :. [MGI Ref ID J:181831]
Animal Health ReportsProduction of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.
Breeding & Husbandry While maintaining a live colony, these mice have been bred as heterozygotes, however, the Donating Investigator indicates that homozygotes are viable and fertile.
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