Strain Name:

B6.Cg-Uba6tm1Whpr/J

Stock Number:

021506

Order this mouse

Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
These Uba6flox/flox mutant mice possess loxP sites flanking exons 16-17 of the targeted gene. This strain may be useful for studying the regulation of protein homeostasis during development.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
 
Donating Investigator Wade Harper,   Harvard University

Description
These Uba6flox/flox mutant mice possess loxP sites flanking exons 16-17 of the ubiquitin-like modifier activating enzyme 6 (Uba6) gene. Uba6 (E1) is part of the ubiquitin proteasome system (UPS) which regulates protein homeostasis during development. Complete UBA6 deficiency leads to embryonic arrest by E5.5. Mice that are homozygous for this floxed-allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 3-6 deleted in the cre-expressing tissues.

For example, when bred to B6.Cg-Tg(Nes-cre)1Kln/J mice (Stock No. 003771), resulting mice lack neuronal UBA6 and display altered neuronal patterning in the hippocampus and the amygdala, decreased dendritic spine density, and numerous behavioral disorders. They also contain altered levels of ubiquitin protein ligase E3A (Ube3a) and SH3/ankyrin domain gene 3 (Shank3) spine proteins.

Development
A targeting vector was designed to insert a loxP site upstream of exon 16, and a second loxP site, followed by a frt-flanked neomycin resistance (neo) cassette, downstream of exon 17 of the ubiquitin-like modifier activating enzyme 6 (Uba) gene. The construct was electroporated into (C57BL/6 x 129/SvEv)F1-derived BA1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to C57BL/6 mice for at least 5 generations. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Control Information

  Control
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Uba6
008744   B6;129-Uba6tm1Zjc/J
View Strains carrying other alleles of Uba6     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Uba6tm1Whpr/Uba6tm1Whpr

        involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6NTac
  • normal phenotype
  • no abnormal phenotype detected
    • mice are viable and fertile   (MGI Ref ID J:101977)

The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.

Uba6tm1Whpr/Uba6tm1Whpr Tg(Nes-cre)1Kln/0

        B6.Cg-Uba6tm1Whpr Tg(Nes-cre)1Kln   (conditional)
  • mortality/aging
  • partial postnatal lethality
    • half of mice die between P5 and P21   (MGI Ref ID J:198126)
  • premature death
    • a quarter of mice that survive to P21 die within 2 months   (MGI Ref ID J:198126)
    • however, lethality is partially rescued by making food more accessible   (MGI Ref ID J:198126)
  • behavior/neurological phenotype
  • abnormal depression-related behavior
    • in a forced swim test, mice spend less time immobilized compared with wild-type mice   (MGI Ref ID J:198126)
  • abnormal habituation to a new environment
    • lack of habituation   (MGI Ref ID J:198126)
  • abnormal nest building behavior
    • aversion to nesting   (MGI Ref ID J:198126)
  • abnormal pilomotor reflex
    • constitutive without an alteration in body temperature   (MGI Ref ID J:198126)
  • abnormal social investigation
    • mice prefer to explore an empty chamber rather than a stranger mouse   (MGI Ref ID J:198126)
  • abnormal spatial working memory
    • in the Y-maze test   (MGI Ref ID J:198126)
  • aphagia
    • absence of stomach content in several mice at death   (MGI Ref ID J:198126)
  • decreased grip strength   (MGI Ref ID J:198126)
  • enhanced coordination
    • slightly better performance on a rotarod   (MGI Ref ID J:198126)
  • hyperactivity
    • in a novel environment with a lack of habituation   (MGI Ref ID J:198126)
    • pronounced in the dark phase   (MGI Ref ID J:198126)
  • impaired contextual conditioning behavior
    • with altered response to an aversive stimulus   (MGI Ref ID J:198126)
  • increased startle reflex   (MGI Ref ID J:198126)
  • increased thigmotaxis   (MGI Ref ID J:198126)
  • nervous system phenotype
  • *normal* nervous system phenotype
    • neuronal apoptosis at E14.5 and P5 is normal   (MGI Ref ID J:198126)
    • abnormal amygdala morphology   (MGI Ref ID J:198126)
    • abnormal dendritic spine morphology
      • reduced density in the CA3 region and amygdala   (MGI Ref ID J:198126)
    • abnormal hippocampus morphology   (MGI Ref ID J:198126)
      • abnormal hippocampus CA3 region morphology
        • scattered neurons   (MGI Ref ID J:198126)
    • decreased brain size   (MGI Ref ID J:198126)
    • decreased neuron number
      • in the CA3 region as early as P5, the basolateral amygdala and piriform cortex   (MGI Ref ID J:198126)
      • however, the number of neurons is normal in the CA1, dentate gyrus, cortex and cerebellar regions   (MGI Ref ID J:198126)
    • decreased prepulse inhibition   (MGI Ref ID J:198126)
  • homeostasis/metabolism phenotype
  • *normal* homeostasis/metabolism phenotype
    • mice exhibit normal body temperature and constant respiratory exchange ratio throughout the diurnal cycle   (MGI Ref ID J:198126)
    • abnormal metabolism
      • hypermetabolic   (MGI Ref ID J:198126)
    • increased carbon dioxide production   (MGI Ref ID J:198126)
    • increased energy expenditure   (MGI Ref ID J:198126)
    • increased oxygen consumption   (MGI Ref ID J:198126)
  • growth/size/body phenotype
  • decreased birth weight   (MGI Ref ID J:198126)
  • decreased body length
    • at birth and 11 weeks   (MGI Ref ID J:198126)
  • decreased body weight   (MGI Ref ID J:198126)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cell Biology Research
Post-translational Processing
Protein Processing
      degradation

Neurobiology Research
Cre-lox System
      loxP-flanked Sequences

Research Tools
Cre-lox System
      loxP-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Uba6tm1Whpr
Allele Name targeted mutation 1, Wade Harper
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) Uba6flox;
Strain of Origin(C57BL/6NTac x 129S6/SvEvTac)F1
Gene Symbol and Name Uba6, ubiquitin-like modifier activating enzyme 6
Chromosome 5
Gene Common Name(s) 5730469D23Rik; AU021846; AW124799; E1-L2; MOP-4; RGD1308324; RIKEN cDNA 5730469D23 gene; UBE1L2; Ube1l2; expressed sequence AU021846; expressed sequence AW124799; ubiquitin-activating enzyme E1-like 2;
Molecular Note A targeting vector was designed to insert a loxP site upstream of exon 16. A second loxP site, followed by a FRT-flanked neomycin resistance (neo) cassette, was inserted downstream of exon 17. [MGI Ref ID J:198126]

Genotyping

Genotyping Information

Genotyping Protocols

Uba6tm1.1Whpr-Alternate 1, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

The Jackson Laboratory. 2005. Information obtained from The Jackson Laboratory, Bar Harbor, ME Unpublished :.  [MGI Ref ID J:101977]

Additional References

Uba6tm1Whpr related

Lee PC; Dodart JC; Aron L; Finley LW; Bronson RT; Haigis MC; Yankner BA; Harper JW. 2013. Altered social behavior and neuronal development in mice lacking the Uba6-Use1 ubiquitin transfer system. Mol Cell 50(2):172-84. [PubMed: 23499007]  [MGI Ref ID J:198126]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice may be bred together.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
- Use of MICE by companies or for-profit entities requires a license.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.6)