Strain Name:

B6.129S6-Plin1tm1Asg/J

Stock Number:

021887

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Availability:

Repository- Live

Homozygotes for this targeted mutation of the Plin1, perilipin 1, gene exhibit decreased adipose mass and adipocyte size, and increased basal lipolysis. This mutant mouse strain may be useful in studies of lipolysis and lipid metabolism.

Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Additional information on Congenic nomenclature.
Mating SystemHeterozygote x Heterozygote         (Female x Male)   23-AUG-13
Specieslaboratory mouse
GenerationN10+pN1 (04-NOV-13)
Generation Definitions
 
Donating Investigator Andrew S. Greenberg,   Tufts University

Description
The Plin1, perilipin 1, gene encodes a protein that protectively coats lipid storage droplets in adipocytes. Mice that are homozygous for this targeted mutation, which disrupts exon 3 with a NEO cassette, are viable and fertile. No gene product (mRNA or protein) is detected by Northern or Western blot analysis. Homozygotes exhibit decreased adipocyte size and reduced adipose mass. Interscapular brown adipose tissue (IBAT) and white adipose tissue depot amount is reduced in homozygotes. In addition, IBAT depots are darker in color, have smaller lipid droplets and 47% less triacylglycerol amounts when compared to wildtype controls. Brown adipocytes isolated from homozygous mice have a higher basal glycerol and fatty acid release rate, which is not enhanced by norepinephrine.

Development
A targeting vector designed by Dr. Andrew S. Greenberg (Tufts University) containing a NEO cassette was used to disrupt exon 3. The construct was electroporated into unspecified 129/SvEvTac derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. Chimeric animals were subsequently generated. The donating investigator reported that these mice were then backcrossed to C57BL/6J for 10 generations (see SNP note below). Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 3 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Plin1
003870   B6.129-Plin1tm1Chan/J
View Strains carrying other alleles of Plin1     (1 strain)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Lipodystrophy, Familial Partial, Type 4; FPLD4   (PLIN1)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Plin1tm1Asg/Plin1tm1Asg

        B6.129S6-Plin1tm1Asg
  • adipose tissue phenotype
  • *normal* adipose tissue phenotype
    • interscapular BAT thermal profile is normal; temperature increases ~3 degrees Celsius with norepinephrine infusion   (MGI Ref ID J:121641)
    • abnormal brown adipose tissue physiology
      • isolated brown adipocytes show increased basal glycerol and fatty acid release rate compared to Plin-null Tg(Fabp4-Plin*)1Asg adipocytes and wild-type adipocytes; norepinephrine does not produce an increase like that seen in stimulated wild-type adipocytes   (MGI Ref ID J:121641)
    • abnormal white adipose tissue amount
      • white adipose tissue (WAT) depots are smaller than those in controls   (MGI Ref ID J:121641)
    • decreased brown adipose tissue amount
      • interscapular BAT depot weight is reduced relative to controls (0.10 g vs 0.15 g in controls)   (MGI Ref ID J:121641)
      • interscapular BAT depots are darker, display smaller lipid droplets, and have 47% lower triacylglycerol content than wild-type mice   (MGI Ref ID J:121641)
  • homeostasis/metabolism phenotype
  • *normal* homeostasis/metabolism phenotype
    • norepinephrine-induced thermal response is similar to that of wild-type mice; core temperature increases ~2 degrees Celsius with norepinephrine infusion   (MGI Ref ID J:121641)
    • abnormal circulating free fatty acids level
      • forskolin-stimulated lipolysis in Plin-null MEFs transfected with adenovirus expressing Plin is increased ~12-fold compared to basal level of lipolysis in null MEFs, whereas forskolin-stimulated lipolysis is in MEFs expressing Plin with mutated phosphokinase A site is increased only to level of lipolysis exhibited by null MEFs transfected with adenoviral GFP vector   (MGI Ref ID J:113735)
    • enhanced lipolysis   (MGI Ref ID J:113735)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Internal/Organ Research
Adipose Defects

Metabolism Research
Lipid Metabolism

Research Tools
Metabolism Research

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Plin1tm1Asg
Allele Name targeted mutation 1, Andrew S Greenberg
Allele Type Targeted (knock-out)
Common Name(s) PeriKO;
Strain of Origin129S6/SvEvTac
Gene Symbol and Name Plin1, perilipin 1
Chromosome 7
Gene Common Name(s) 6030432J05Rik; FPLD4; PERI; PERIA; PLIN; Plin; RIKEN cDNA 6030432J05 gene; perilipin; perilipin A; perilipin B;
Molecular Note Exon 3 (nucleotides 3515-3887) was replaced with a neomycin resistance cassette, thereby disrupting the coding of all known mRNAs. [MGI Ref ID J:113735]

Genotyping

Genotyping Information

Genotyping Protocols

Plin1tm1Asg, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Miyoshi H; Souza SC; Zhang HH; Strissel KJ; Christoffolete MA; Kovsan J; Rudich A; Kraemer FB; Bianco AC; Obin MS; Greenberg AS. 2006. Perilipin promotes hormone-sensitive lipase-mediated adipocyte lipolysis via phosphorylation-dependent and -independent mechanisms. J Biol Chem 281(23):15837-44. [PubMed: 16595669]  [MGI Ref ID J:113735]

Additional References

Plin1tm1Asg related

Souza SC; Christoffolete MA; Ribeiro MO; Miyoshi H; Strissel KJ; Stancheva ZS; Rogers NH; D'Eon TM; Perfield JW 2nd; Imachi H; Obin MS; Bianco AC; Greenberg AS. 2007. Perilipin regulates the thermogenic actions of norepinephrine in brown adipose tissue. J Lipid Res 48(6):1273-9. [PubMed: 17401109]  [MGI Ref ID J:121641]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX18

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as homozygotes.
Mating SystemHeterozygote x Heterozygote         (Female x Male)   23-AUG-13
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHeterozygous for Plin1tm1Asg  
$232.00Female or MaleHomozygous for Plin1tm1Asg  
Price per Pair (US dollars $)Pair Genotype
$464.00Heterozygous for Plin1tm1Asg x Heterozygous for Plin1tm1Asg  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHeterozygous for Plin1tm1Asg  
$301.60Female or MaleHomozygous for Plin1tm1Asg  
Price per Pair (US dollars $)Pair Genotype
$603.20Heterozygous for Plin1tm1Asg x Heterozygous for Plin1tm1Asg  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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