Strain Name:


Stock Number:



On Hold

Register Interest
FGF21 transgenic mice have the apolipoprotein E (ApoE) promoter/enhancer sequences driving expression of fibroblast growth factor 21 (Fgf21). These mice may be useful for studying the role of FGF21 in coordinating metabolic responses to fasting.


Strain Information

Type Coisogenic; Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
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Mating SystemNoncarrier x Hemizygote         (Female x Male)   07-MAY-14
Specieslaboratory mouse
GenerationN10+pN1 (31-MAR-14)
Generation Definitions
Donating Investigator Steven A Kliewer,   UTSouthwestern Medical Center

FGF21 transgenic mice have the murine apolipoprotein E (ApoE) promoter/enhancer sequences driving expression of fibroblast growth factor 21 (Fgf21) in liver. Mice from founder line 1, containing a 50 fold increase in Fgf21 mRNA levels over fasted wildtype mice, were established. FGF21 is an endocrine hormone, expressed in liver, pancreas, and adipose tissue, which stimulates glucose uptake in adipocytes. FGF21 is induced by peroxisome proliferator-activated receptor a (PPARa) in liver during fasting and induces hepatic fatty acid oxidation and ketogenesis. Hemizygotes are viable, with only male transgenic mice are fertile. Overexpression of FGF21 extends the life span of these FGF21 transgenic mice. They have significant reductions in serum cholesterol, glucose, and insulin. In the liver, they have increased oxygen consumption, beta oxidation, gluconeogenesis, and glycogen content. They have decreased serum and hepatic triglyceride concentrations. In these mice, mRNAs encoding pancreatic lipases are increased in liver and adipocyte size is decrease. These mice are more sensitive to torpor during a 24 hour fast.

The FGF21 transgene was designed with a murine fibroblast growth factor 21 (Fgf21) cDNA driven by murine apolipoprotein E (ApoE) promoter/enhancer sequences. The transgene was microinjected into fertilized C57BL/6NCrl oocytes and mice from founder line 1 were bred to C57BL/6J mice to establish a colony. These mice were bred to C57BL/6J mice for at least ten generations. Upon arrival at The Jackson Laboratory, transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) for one generation.

Control Information

   000664 C57BL/6J (approximate)
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of Apoe     (19 strains)

Strains carrying other alleles of Fgf21
022361   B6.129S6(SJL)-Fgf21tm1.2Djm/J
View Strains carrying other alleles of Fgf21     (1 strain)


Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.


        Background Not Specified
  • homeostasis/metabolism phenotype
  • abnormal gluconeogenesis
    • under fed conditions, liver gluconeogenesis is increased 60% compared to in similarly treated wild-type mice   (MGI Ref ID J:150831)
    • however, liver gluconeogenesis does not increase further under fasting conditions   (MGI Ref ID J:150831)
  • increased glycogen level
    • liver glycogen content increased 50% compared to in wild-type mice   (MGI Ref ID J:150831)
  • increased oxygen consumption
    • hepatic oxygen consumption is increased in fasting mice compared with wild-type mice   (MGI Ref ID J:150831)
    • however, hepatic oxygen consumption under fed condition is normal   (MGI Ref ID J:150831)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cardiovascular Research

Developmental Biology Research
Internal/Organ Defects

Diabetes and Obesity Research

Endocrine Deficiency Research
Adipose Defects
Pancreas Defects

Internal/Organ Research
Adipose Defects
Liver Defects

Metabolism Research
Enzyme Deficiency
      exocrine pancreatic insufficiency
Low Body Temperature

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol Tg(Apoe-Fgf21)1Sakl
Allele Name transgene insertion 1, Steven A Kliewer
Allele Type Transgenic (Inserted expressed sequence)
Common Name(s) Tg(Fgf21);
Strain of OriginC57BL/6NCrl
Expressed Gene Fgf21, fibroblast growth factor 21, mouse, laboratory
Promoter Apoe, apolipoprotein E, mouse, laboratory
Molecular Note The mouse promoter was used to drive liver-specific expression of the mouse cDNA. Two lines were created (1 and 2) that result in 50-fold, and 150-fold, respectively, higher expression of the transcript compared to in wild-type mice. The two lines exhibit virtually identical changes in fasting serum beta-hydroxybutyrate, triglyceride, total cholesterol, glucose, and insulin concentrations. Line 1 was selected as the representative line. [MGI Ref ID J:129855]


Genotyping Information

Genotyping Protocols

Tg(Apoe-Fgf21)1Sakl-Alternate1, Standard PCR

Helpful Links

Genotyping resources and troubleshooting


References provided by MGI

Selected Reference(s)

Inagaki T; Dutchak P; Zhao G; Ding X; Gautron L; Parameswara V; Li Y; Goetz R; Mohammadi M; Esser V; Elmquist JK; Gerard RD; Burgess SC; Hammer RE; Mangelsdorf DJ; Kliewer SA. 2007. Endocrine Regulation of the Fasting Response by PPARalpha-Mediated Induction of Fibroblast Growth Factor 21. Cell Metab 5(6):415-25. [PubMed: 17550777]  [MGI Ref ID J:129855]

Additional References

Tg(Apoe-Fgf21)1Sakl related

Bookout AL; de Groot MH; Owen BM; Lee S; Gautron L; Lawrence HL; Ding X; Elmquist JK; Takahashi JS; Mangelsdorf DJ; Kliewer SA. 2013. FGF21 regulates metabolism and circadian behavior by acting on the nervous system. Nat Med 19(9):1147-52. [PubMed: 23933984]  [MGI Ref ID J:201888]

Ding X; Boney-Montoya J; Owen BM; Bookout AL; Coate KC; Mangelsdorf DJ; Kliewer SA. 2012. betaKlotho is required for fibroblast growth factor 21 effects on growth and metabolism. Cell Metab 16(3):387-93. [PubMed: 22958921]  [MGI Ref ID J:189018]

Owen BM; Ding X; Morgan DA; Coate KC; Bookout AL; Rahmouni K; Kliewer SA; Mangelsdorf DJ. 2014. FGF21 acts centrally to induce sympathetic nerve activity, energy expenditure, and weight loss. Cell Metab 20(4):670-7. [PubMed: 25130400]  [MGI Ref ID J:215567]

Pashkov V; Huang J; Parameswara VK; Kedzierski W; Kurrasch DM; Tall GG; Esser V; Gerard RD; Uyeda K; Towle HC; Wilkie TM. 2011. Regulator of G Protein Signaling (RGS16) Inhibits Hepatic Fatty Acid Oxidation in a Carbohydrate Response Element-binding Protein (ChREBP)-dependent Manner. J Biol Chem 286(17):15116-25. [PubMed: 21357625]  [MGI Ref ID J:172091]

Potthoff MJ; Inagaki T; Satapati S; Ding X; He T; Goetz R; Mohammadi M; Finck BN; Mangelsdorf DJ; Kliewer SA; Burgess SC. 2009. FGF21 induces PGC-1alpha and regulates carbohydrate and fatty acid metabolism during the adaptive starvation response. Proc Natl Acad Sci U S A 106(26):10853-8. [PubMed: 19541642]  [MGI Ref ID J:150831]

Wei W; Dutchak PA; Wang X; Ding X; Wang X; Bookout AL; Goetz R; Mohammadi M; Gerard RD; Dechow PC; Mangelsdorf DJ; Kliewer SA; Wan Y. 2012. Fibroblast growth factor 21 promotes bone loss by potentiating the effects of peroxisome proliferator-activated receptor gamma. Proc Natl Acad Sci U S A 109(8):3143-8. [PubMed: 22315431]  [MGI Ref ID J:182005]

Zhang Y; Xie Y; Berglund ED; Coate KC; He TT; Katafuchi T; Xiao G; Potthoff MJ; Wei W; Wan Y; Yu RT; Evans RM; Kliewer SA; Mangelsdorf DJ. 2012. The starvation hormone, fibroblast growth factor-21, extends lifespan in mice. Elife 1:e00065. [PubMed: 23066506]  [MGI Ref ID J:197581]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX18

Colony Maintenance

Breeding & HusbandryHemizygous females are infertile. When maintaining a live colony, wildtype (noncarrier) females from the colony or C57BL/6J females may be bred with hemizygous males.
Mating SystemNoncarrier x Hemizygote         (Female x Male)   07-MAY-14

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


This strain is currently On Hold.
Register Interest

To request more information use the Customer Support Contact Form or call 1-800-422-6423 or 1-207-288-5845

View All Strains Awaiting Transfer from the Donor, In Progress and On Hold

Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00MaleHemizygous for Tg(Apoe-Fgf21)1Sakl  
Price per Pair (US dollars $)Pair Genotype
$304.00Noncarrier x Hemizygous for Tg(Apoe-Fgf21)1Sakl  

Standard Supply

This strain is currently on HOLD - Contact Customer Service for more information.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60MaleHemizygous for Tg(Apoe-Fgf21)1Sakl  
Price per Pair (US dollars $)Pair Genotype
$395.20Noncarrier x Hemizygous for Tg(Apoe-Fgf21)1Sakl  

Standard Supply

This strain is currently on HOLD - Contact Customer Service for more information.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

This strain is currently on HOLD - Contact Customer Service for more information.

General Supply Notes

  • Due to decreased productivity this strain [JR.21964] is undergoing expansion and will be on hold for approximately 3 months.

Control Information

   000664 C57BL/6J (approximate)
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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