Strain Name:

STOCK Smad2tm1.1Epb/J

Stock Number:

022074

Order this mouse

Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
These Smad2f/f mice possess loxP sites flanking exon 2 of the SMAD family member 2 gene. These mice may be useful for studying the cellular and mechanical role of TGF-β in regulating development.

Description

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Mating SystemHeterozygote x Heterozygote         (Female x Male)   28-JUL-13
Specieslaboratory mouse
Generation?+pN2 (12-DEC-13)
Generation Definitions
 
Donating Investigator Erwin P. Bottinger,   The Mount Sinai Hospital

Description
These Smad2f/f mice possess loxP sites flanking exon 2 of the SMAD family member 2 (Smad2) gene. SMAD2 is an intracellular signaling mediators involved in growth control and transcriptional regulation during development by transmitting chemical signals from the cell surface to the nucleus via the transforming growth factor β (TGFβ) signaling system. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in cre-expressing tissues.

For example, when crossed to B6.Cg-Tg(Cdh16-cre)91Igr/J mice (Stock No. 012237) expressing Cre recombinase in the kidney, TGF-β signaling is enhanced which promotes increased fibrosis.

When crossed to B6.Cg-Tg(Alb-cre)21Mgn/J mice (Stock No. 003574) expressing Cre recombinase in the liver, hepatocytes exhbit altered growth and dedifferentiation independent of TGF-beta signaling.

Development
A targeting vector was designed to insert a loxP-flanked neomycin resistance (neo) cassette upstream of exon 2, and a single loxP site downstream of exon 2 of the SMAD family member 2 (Smad2) gene. The construct was electroporated into STOCK (129/Sv and C57BL/6J and SJL)-derived WW6 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric males were bred with C57BL/6 x 129V females. Offspring were bred with CMV-cre transgenic mice to delete the neo cassette. Resulting progeny contained multiple gene rearrangments; intact floxed-exon 2, intact floxed-neo cassette, or excision of both exon 4 and the neo cassette. Mice, containing only the floxed-exon 2, were bred with C57BL/6J mice. Upon arrival, Smad2f/f mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Control Information

  Control
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.

Smad2tm1.1Epb/Smad2tm1.1Epb Tg(Alb-cre)21Mgn/0

        involves: 129/Sv * C57BL/6 * DBA * SJL   (conditional)
  • liver/biliary system phenotype
  • *normal* liver/biliary system phenotype
    • mice exhibit normal postnatal liver and body growth and function   (MGI Ref ID J:119642)
  • cellular phenotype
  • abnormal cell morphology
    • after attachment to culture dishes, hepatocytes are elongated with process formation and cell separation compared to wild-type cells which have a cobblestone epithelial morphology   (MGI Ref ID J:119642)
  • abnormal cell physiology
    • hepatocytes express much higher levels of vimentin (mesenchymal marker) compared to control or Smad3 mutant cells   (MGI Ref ID J:119642)
    • abnormal cell differentiation
      • hepatocytes dedifferentiate spontaneously to acquire mesenchymal and promigratory features in culture   (MGI Ref ID J:119642)
    • abnormal cell migration
      • compared to wild-type cells which cannot migrate to the wound area in a scratch-wound in vitro assay after 24 hours or only partial cover area when treated with TGFbeta, mutant cells cover the entire scratch area in absence of TGFbeta   (MGI Ref ID J:119642)
    • increased cell proliferation
      • in culture, untreated cells exhibit ~3-fold greater proliferation compared to controls and Smad3 hepatocytes; after TGFbeta treatement, cells show reduced proliferation compared to controls   (MGI Ref ID J:119642)
      • in CCL4-injured livers, cell proliferation is higher at earlier time points than in controls or Smad3-deficient livers   (MGI Ref ID J:119642)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cell Biology Research
Genes Regulating Growth and Proliferation
Signal Transduction
Transcriptional Regulation

Research Tools
Cre-lox System
      loxP-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Smad2tm1.1Epb
Allele Name targeted mutation 1.1, Erwin P Bottinger
Allele Type Targeted (Floxed/Frt)
Common Name(s) Smad2f;
Strain of OriginSTOCK 129/Sv and C57BL/6J and SJL
Gene Symbol and Name Smad2, SMAD family member 2
Chromosome 18
Gene Common Name(s) 7120426M23Rik; JV18; JV18-1; MAD homolog 2, (Drosophila); MADH2; MADR2; Madh2; RIKEN cDNA 7120426M23 gene; Smad 2; hMAD-2; hSMAD2;
Molecular Note Exon 2, which contains the ATG, remained flanked by loxP sites following in vivo cre-mediated recombination by crossing floxed mice with cre mice. A floxed pgk-neomycin resistance cassette was removed from the locus. [MGI Ref ID J:119642]

Genotyping

Genotyping Information

Genotyping Protocols

Smad2tm1.1EpbAlternate1, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Ju W; Ogawa A; Heyer J; Nierhof D; Yu L; Kucherlapati R; Shafritz DA; Bottinger EP. 2006. Deletion of Smad2 in mouse liver reveals novel functions in hepatocyte growth and differentiation. Mol Cell Biol 26(2):654-67. [PubMed: 16382155]  [MGI Ref ID J:119642]

Additional References

Smad2tm1.1Epb related

Chung AC; Huang XR; Meng X; Lan HY. 2010. miR-192 mediates TGF-beta/Smad3-driven renal fibrosis. J Am Soc Nephrol 21(8):1317-25. [PubMed: 20488955]  [MGI Ref ID J:185933]

El-Gohary Y; Tulachan S; Guo P; Welsh C; Wiersch J; Prasadan K; Paredes J; Shiota C; Xiao X; Wada Y; Diaz M; Gittes G. 2013. Smad signaling pathways regulate pancreatic endocrine development. Dev Biol 378(2):83-93. [PubMed: 23603491]  [MGI Ref ID J:198451]

Hoot KE; Lighthall J; Han G; Lu SL; Li A; Ju W; Kulesz-Martin M; Bottinger E; Wang XJ. 2008. Keratinocyte-specific Smad2 ablation results in increased epithelial-mesenchymal transition during skin cancer formation and progression. J Clin Invest 118(8):2722-32. [PubMed: 18618014]  [MGI Ref ID J:140425]

Hoot KE; Oka M; Han G; Bottinger E; Zhang Q; Wang XJ. 2010. HGF upregulation contributes to angiogenesis in mice with keratinocyte-specific Smad2 deletion. J Clin Invest 120(10):3606-16. [PubMed: 20852387]  [MGI Ref ID J:165261]

Meng XM; Huang XR; Chung AC; Qin W; Shao X; Igarashi P; Ju W; Bottinger EP; Lan HY. 2010. Smad2 protects against TGF-beta/Smad3-mediated renal fibrosis. J Am Soc Nephrol 21(9):1477-87. [PubMed: 20595680]  [MGI Ref ID J:185921]

Rajagopal R; Dattilo LK; Kaartinen V; Deng CX; Umans L; Zwijsen A; Roberts AB; Bottinger EP; Beebe DC. 2008. Functions of the type 1 BMP receptor Acvr1 (Alk2) in lens development: cell proliferation, terminal differentiation, and survival. Invest Ophthalmol Vis Sci 49(11):4953-60. [PubMed: 18566469]  [MGI Ref ID J:141762]

Taniguchi K; Anderson AE; Sutherland AE; Wotton D. 2012. Loss of Tgif function causes holoprosencephaly by disrupting the SHH signaling pathway. PLoS Genet 8(2):e1002524. [PubMed: 22383895]  [MGI Ref ID J:183402]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony mice homozygous for the floxed allele may be bred together.
Mating SystemHeterozygote x Heterozygote         (Female x Male)   28-JUL-13
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHeterozygous for Smad2tm1.1Epb  
$232.00Female or MaleHomozygous for Smad2tm1.1Epb  
Price per Pair (US dollars $)Pair Genotype
$464.00Heterozygous for Smad2tm1.1Epb x Heterozygous for Smad2tm1.1Epb  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHeterozygous for Smad2tm1.1Epb  
$301.60Female or MaleHomozygous for Smad2tm1.1Epb  
Price per Pair (US dollars $)Pair Genotype
$603.20Heterozygous for Smad2tm1.1Epb x Heterozygous for Smad2tm1.1Epb  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Control Information

  Control
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.5)